Labshake search
Citations for Bioline :
401 - 450 of 1604 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: ... for 1 hour at 37°C and proteinase K (40 ug, Bioline BIO-37084) for 2 hours at 55°C ...
-
bioRxiv - Genomics 2023Quote: ... PCR products were purified using the ISOLATE II PCR and Gel Kit (Bioline, BIO-52060) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... and cDNA was synthesized (Bioline) using random hexamers as previously described (16,18,78) ...
-
bioRxiv - Microbiology 2023Quote: ... using the SensiFAST SYBR & Fluorescein Kit (Bioline), on a Roche Lightcycler 96 ...
-
bioRxiv - Microbiology 2023Quote: ... before synthesis of cDNA using random hexamers (Bioline; Edwards, Nawrocki et al. 2014). Quantitative reverse-transcription PCR (qRT-PCR ...
-
bioRxiv - Genomics 2023Quote: ... PCR products purified with Isolate II PCR and Gel Kit (BIOLINE) and cloned into the entry vector pCR®8/GW/TOPO (#250020 Invitrogen ...
-
bioRxiv - Cell Biology 2023Quote: ... Cas9 target sites within the AAVS1 locus were PCR amplified in a 25 or 50 μL reaction volume per sample using high-performance RANGER PCR Mix (Bioline USA, Inc., Memphis, TN, USA) or PlatinumTM SuperFiTM PCR Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was performed using the SensiFAST SYBR Lo-ROX Kit (BIO-94020, Bioline). Primers are shown in Supplementary Table 1 ...
-
bioRxiv - Genomics 2023Quote: ... 1’ 72°C) by MyTaq Red mix (Bioline) using primers that add overhangs with EcoRI (MT024 ...
-
bioRxiv - Genomics 2023Quote: ... cells were harvested and resuspended in 800 μl TRIsure (#BIO-38032; Bioline) and stored at -80 °C until further use ...
-
bioRxiv - Microbiology 2023Quote: ... 300 ng of RNA was used to generate cDNA with SensiFAST synthesis reagents (Bioline). Quantitative PCR was performed with Fast SYBR green (Applied Biosystems ...
-
bioRxiv - Microbiology 2023Quote: ... Quantitative reverse-transcription PCR (qRT-PCR) with was performed with three technical replicates using 50 ng cDNA and the SensiFAST SYBR & Fluorescein Kit (Bioline) on a Roche Lightcycler 96 instrument ...
-
bioRxiv - Genetics 2023Quote: ... An 800–base pair region surrounding the target site of the Cypor sgRNA was amplified (forward primer 5′-GTTTGCGGGTGTTAGCTCTTC-3′; reverse primer 5′-TTGGTGGGTAAATCACACCGT-3′) using MyTaq Red Mix (Bioline). The amplicon was purified using the PCR clean-up and gel extraction kit (Macherey-Nagel ...
-
bioRxiv - Genomics 2023Quote: ... Two microliters of cDNA was used for subsequent amplification using SensiFAST SYBR No-Rox Kit (Bioline) on an Eco™ Real-Time PCR System (Ilumina) ...
-
bioRxiv - Plant Biology 2023Quote: ... using SensiFAST SYBR No-ROS kit (Bioline, BIO-98020) following this program ...
-
bioRxiv - Plant Biology 2023Quote: ... purified and converted to cDNA with sensiFAST cDNA Synthesis Kit (Bioline), using the provided mix of random hexamers and anchored oligo dT primers ...
-
bioRxiv - Microbiology 2023Quote: ... with MyTaqTM HS Red Mix (Bioline), thermocycling conditions used were as follows ...
-
bioRxiv - Genetics 2023Quote: Ostrinia nubilalis Hbn.eggs were obtained from Bioline AgroSciences (France) ...
-
bioRxiv - Cancer Biology 2023Quote: ... and cDNA synthesized using SensiFAST cDNA synthesis kit (Bioline). Samples were then amplified against transcripts by qRT-PCR with SYBR green ...
-
bioRxiv - Microbiology 2023Quote: Total RNA was obtained from lyophilized mycelial samples using TRIsureTM (Meridian Bioscience, Bioline), following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... 150 ng of total RNA was converted to complementary DNA (cDNA) using SensiFASTTM cDNA synthesis kit following manufacturer instructions (Bioline) and qPCR performed using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline) ...
-
bioRxiv - Developmental Biology 2023Quote: ... 1.5 ul of DNA was used in MyTaq™ Red Mix (Bioline) PCR amplification and samples were genotyped using primers from Kobayashi et al ...
-
bioRxiv - Cell Biology 2023Quote: ... Supernatant was subsequently used for RNA extraction with the RNA isolation kit from Bioline as described above ...
-
bioRxiv - Genetics 2023Quote: ... Trisure (Bioline) was used for RNA extraction ...
-
bioRxiv - Genetics 2023Quote: ... while qPCR reactions were performed with SensiFAST™ SYBR® No-ROX Kit (Bioline). For oligonucleotide sequences ...
-
bioRxiv - Genetics 2023Quote: ... coli cells (NEB C2987 or Bioline BIO-85027). Bacterial clones were prepped with a midiprep kit ...
-
bioRxiv - Cell Biology 2023Quote: ... with the SensiFAST SYBR No-ROX Kit (Bioline) and gene-specific primers (Supp ...
-
bioRxiv - Microbiology 2023Quote: ... Indexing PCRs were created by combining 10 μL of purified DNA with 10 μL 2x MyTaq HS Mix polymerase (Bioline) and 1 μl (5 μM ...
-
bioRxiv - Immunology 2023Quote: ... The total qPCR reaction volume was 1.5 μl and consisted of 0.5 μl of cDNA (dilution 1/12) and 1 μl of SensiFAST™ SYBR® No-ROX Kit (Bioline) containing 0.4 μM of PCR primer (Eurogenetec SA)(primer list in sup ...
-
bioRxiv - Immunology 2023Quote: ... were recruited if they had a capillary haemoglobin of < 10 g/dL (i.e., moderate or severe anaemia) and a negative malaria rapid diagnostic test (mRDT, SD Bioline Malaria AG Pf/Pan ...
-
bioRxiv - Biochemistry 2023Quote: ... using TaqMan™ Gene Expression Assays (FAM-labelled) and the SensiFASTTM Probe Hi-ROX kit (Bioline). The following TaqMan™ Gene Expression Assays were used ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and 1 kb Hyperladder (Bioline). PCR products were purified using Mag-Bind RXNPurePlus beads (OMEGA Biotek ...
-
bioRxiv - Neuroscience 2023Quote: ... 1 ng of the total RNA was reverse-transcribed into cDNA using a Tetro cDNA synthesis kit (BIO-65042, Bioline, London, UK). Quantitative PCR (RT-qPCR ...
-
bioRxiv - Developmental Biology 2023Quote: ... For real-time PCR SYBR Green Master mix (Bioline BIO-94020) was used ...
-
bioRxiv - Plant Biology 2023Quote: ... and the 200 to 400 bp range was purified by gel extraction using Isolate II PCR and Gel Kit (Bioline). Libraries were sequenced (100 bp paired-end ...
-
bioRxiv - Plant Biology 2023Quote: ... A total of 1 µg of total RNA was used to initiate cDNA synthesis using the SensiFAST™ cDNA Synthesis Kit (Bioline, London, UK). The SYBR Green assay with SensiFAST™ SYBR no ROX kit was used for the quantification of transcripts in RT-PCR ...
-
bioRxiv - Plant Biology 2023Quote: ... and further purified with Isolate II Gel and PCR Clean-up Kit (Bioline). Libraries were then generated from fragmented DNA (Covaris ...
-
bioRxiv - Microbiology 2023Quote: ... 0.2 ml chloroform was added for phase separation using phase lock gel heavy tubes and RNA isolation from the aqueous phase was obtained by using ISOLATE II RNA Mini Kit (Bioline). RNA quality was verified with Agilent 2100 Bioanalyzer System using RNA Nano Chips (Agilent Technologies) ...
-
bioRxiv - Neuroscience 2023Quote: ... and reverse transcribed using the SensiFast cDNA Synthesis Kit (Bioline). Quantitative real-time PCR was performed in triplets for each sample using the LightCycler DNA Master SYBR Green I Kit in a LightCycler 480 System (Roche) ...
-
bioRxiv - Microbiology 2023Quote: ... If a band was visualised at ∼480 bp the PCR product was purified using an ISOLATE II PCR and Gel Kit (Bioline).
-
bioRxiv - Microbiology 2023Quote: ... DNA was extracted using the ISOLATE II Genomic DNA Kit (Bioline). Briefly ...
-
bioRxiv - Microbiology 2023Quote: ... PCR products that produced bands of the correct size were purified using the ISOLATE II PCR and Gel Kit (Bioline) as per manufacturer’s protocol and submitted for sequencing using an Applied Biosystems 3730xl capillary analyser (Macrogen) ...
-
bioRxiv - Microbiology 2023Quote: ... DNA was extracted from the FTATM card using an ISOLATE II Genomic DNA Kit (Bioline) with a pre-lysis in 180 µL of Lysis Buffer GL and 25 µL Proteinase K for 2 hours before completing the extraction as per manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2023Quote: ... Quantitative real-time PCR reactions were performed with different sets of primers and Sensifast SYBR (Bioline, Taunton, MA) on a CFX96 or CFX384 Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Cancer Biology 2023Quote: ... Genotyping PCR on genomic DNA was performed using the MyTaq DNA Polymerase system (Bioline, cat. no. BIO-21105). Briefly ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR was performed using MyTaq HS Red Mix (Bioline) with 66°C annealing and 15s elongation ...
-
bioRxiv - Microbiology 2023Quote: ... Indexing PCRs were created by combining 10 μl of purified DNA with 10 μl 2x MyTaq HS Mix polymerase (Bioline, Narellan, NSW, Australia) and 1 μl (5 μM ...
-
bioRxiv - Molecular Biology 2023Quote: Cells were harvested in TRIsure (Bioline #38033). RNA was extracted using Direct-zol RNA MicroPrep columns (Zymo #R2062 ...
-
bioRxiv - Microbiology 2023Quote: ... was performed in technical triplicate with 50 ng of cDNA per reaction using the SensiFAST SYBR & Fluorescein Kit from Bioline, on a Roche Lightcycler 96 ...
-
bioRxiv - Molecular Biology 2023Quote: ... coli (TOP10- or Stbl3) using Isolate II plasmid miniprep kit (Bioline).