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Citations for Bioline :
1 - 50 of 1604 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was generated from 1 µg of RNA using the SensiFAST cDNA synthesis kit (Bioline, #BIO65054). cDNA was then diluted (1:5 ...
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bioRxiv - Molecular Biology 2024Quote: ... real-time PCR was conducted using the SensiFAST SYBR real-time PCR kit (Bioline). The obtained data were normalized to the human endogenous control (GAPDH ...
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bioRxiv - Molecular Biology 2024Quote: ... the Methyl Indexed PCR was performed by mixing 4 µL of ligated DNA with x-Gen Dual combinatorial Indexes (IDT) (125 nM final concentration) and MyTaq RedMix (Bioline, BIO-25048) in a final volume of 40 µL ...
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bioRxiv - Molecular Biology 2024Quote: ... All three PCR reactions contain 10 µL of MyTaq HS Red mix (BIO-25048, Bioline), 2-3 µL of the crude extract and 1 µL of each primer (10 µM ...
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bioRxiv - Molecular Biology 2024Quote: ... After selection on LB-agar kanamycin plates the resulting colonies were first screened by colony PCR using MyTaq DNA polymerase (Bioline) to check for the presence of the right size insert using T7forward and T7reverese primers ...
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bioRxiv - Cell Biology 2024Quote: RNA from cell pellets treated with dTAG-13 for four hours was isolated using the Isolate II RNA Mini Kit (Bioline BIO-52072), and 1 μg was converted to cDNA using the qScript cDNA Synthesis Kit (VWR 101414) ...
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bioRxiv - Biophysics 2024Quote: ... Total RNA was extracted from both cells and tissue powder by TRIsure reagent (Bioline, Ecogen, BIO-38033) following the manufacturer’s protocol and using phenol-chloroform ...
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bioRxiv - Pathology 2024Quote: ... then incubated overnight at 37°C with staining buffer (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside [X-gal, Bioline, London ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were separated by gel electrophoresis on 2% DNA grade agarose (Bioline) gels in TAE buffer (40 mM Tris Acetate ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed using the SensiFast Sybr No-Rox Mix (Bioline) on a RealPlex 2 Thermocycler (Eppendorf).
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bioRxiv - Genetics 2024Quote: RT-qPCR was performed (in triplicate) using the Bio-Rad cycler CFX 96 and the SensiFAST SYBR Green Kit (Catalog Number BIO-98005, BIOLINE, USA). Gene expression levels were normalized to housekeeping genes pmp-3 ...
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bioRxiv - Cancer Biology 2024Quote: ... Single organoids were hand-picked with capillary pipet tips and examined for locus deletion by PCR using MyTaq™ Red Mix (Bioline, #BIO-25043) and the following cycling parameters ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantitative RT-PCR analyses were performed using SensiFAST™ SYBR® kit (Bioline) on an Applied Biosystems StepOne Plus instrument ...
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bioRxiv - Neuroscience 2024Quote: ... and cDNA synthesis was performed using a Tetro cDNA synthesis kit (Bioline) following the manufacturer’s instructions using the GS-1 G-Storm thermocycler (LabTech) ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 0.16 U/μl Ribosafe RNase inhibitors (Bioline), 2 mM PMSF and cOmplete™ EDTA-free Protease Inhibitor Cocktail and lysed for 10 min on ice before refrigerated centrifugation at 17,000g for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.16 U/μl RNase inhibitor (Bioline), 2 mM PMSF and cOmplete™ EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2024Quote: ... A volume of GRASPS buffer A1 containing 0.16 U/μl RNase inhibitor (Bioline), 2 mM PMSF and cOmplete™ EDTA-free Protease Inhibitor Cocktail corresponding to approximately three times the volume of the cell pellet (600 μl ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteinase K was added to a final concentration of 100 μg/ml (1.25 μl of stock at 20 mg/ml) with 0.16 U/μl Ribosafe RNase inhibitor (Bioline). The reactions were incubated at 37°C for 30 min after pulse vortex to digest the ribosomal proteins ...
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bioRxiv - Microbiology 2024Quote: ... 4.2 µL of extracted DNA was combined with 5 µL of MyTaq Red polymerase (Bioline, Meridian Bioscience), 0.4 µL of 10 uM forward primer (nexF-N3-6-515f for 16S or nexF-N3-6-ITS1f-KYO1 for ITS) ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.25– 1 µg of RNA was reverse-transcribed into cDNA using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054). Real-time PCR was performed on the CFX384 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: ... gondii 529 bp repeat region using the SensiFAST Probe No-ROX Kit (Bioline, BIO-86005) and CFX384 Real-Time System (Bio-Rad ...
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bioRxiv - Immunology 2024Quote: Parasite genomic DNA was isolated from mouse peritoneal exudate cells and whole brain using the Isolate II Genomic DNA Kit (Bioline, BIO-52067). Prior to isolation ...
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bioRxiv - Molecular Biology 2024Quote: The library preparation was performed as follows: genomic DNA was isolated (Bioline, bio-52067) and ∼ 500 ng were digested with DpnI (10 U ...
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bioRxiv - Plant Biology 2024Quote: ... 2.5 uL ImmoBuffer™ (Bioline) 103 ...
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bioRxiv - Plant Biology 2024Quote: ... 0.125 uL Immolase™ DNA polymerase (Bioline), 10 uL DNA for a total of 10ng input ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative Real-time PCR (qRT-PCR) was carried out using SensiFAST™ SYBR® No-ROX One-Step Kit (Bioline) and the results were analysed using the 2−ΔΔCt method (Livak & Schmittgen ...
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bioRxiv - Cell Biology 2024Quote: ... Genotyping PCRs were performed with MyTaq HS Red Mix (Bioline), using primers surrounding the genomic target sites ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1mM dNTP Mix (Bioline), 40 units of Ribosafe RNAse Inhibitor (Bioline) ...
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bioRxiv - Physiology 2024Quote: ... using SensiFAST SYBR No-ROX Kit (Cat no. BIO-98050, Bioline, Froggabio, Canada). The amplification reaction contained 1 μL of diluted cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... treated RNA was converted to cDNA using BioScript Reverse Transcriptase (Bioline) with (dN)6 random primers or dT18 primers as described in40 ...
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bioRxiv - Plant Biology 2024Quote: ... The qRT-PCR amplification was performed with SensiMixTM SYBR NO-Rox Kit (Bioline) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2024Quote: ... PCR was performed using MyTaq (Bioline) DNA Polymerase in reactions containing 30ng of DNA template ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were prepared for qPCR in technical triplicates in 10-µl reaction volumes using SensiFAST SYBR Lo-ROX 2X Master Mix (Bioline; Cat. No. BIO-94005), custom qPCR primers from Integrated DNA Technologies used at a final concentration of 0.2LµM and cDNA diluted at 1:3 ...
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bioRxiv - Neuroscience 2024Quote: ... Complementary DNA was synthesized with the SensiFAST cDNA Synthesis Kit (Bioline; Cat. No. 65054). Samples were prepared for qPCR in technical triplicates in 10-µl reaction volumes using SensiFAST SYBR Lo-ROX 2X Master Mix (Bioline ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 40 units of Ribosafe RNAse Inhibitor (Bioline), and 4µl of 10X random primers (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... we used the ISOLATE II RNA mini kit including on-column DNA digestion (Bioline, UK). The concentrations were measured by NanoDrop ND-1000 (Peqlab ...
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bioRxiv - Neuroscience 2024Quote: ... we used the SensiFAST SYBR Lo-ROX one step kit (Bioline, UK) and the ΔΔCt method with GAPDH serving as a housekeeping gene (Reinhardt et al ...
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bioRxiv - Neuroscience 2024Quote: ... and qPCR performed in technical triplicate using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline) on QuantStudioTM 5 Real-Time PCR system as previously described (34,35,37,41) ...
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bioRxiv - Neuroscience 2024Quote: ... 150 ng of total RNA was converted to complementary DNA (cDNA) using SensiFASTTM cDNA synthesis kit following manufacturer instructions (Bioline) and qPCR performed in technical triplicate using SensiFAST™ SYBR® Lo-ROX Kit following manufacturer instructions (Bioline ...
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bioRxiv - Developmental Biology 2024Quote: ... using the Sensifast SYBR no Rox kit (Bioline, BIO-98020). Data analysis and visualisation was performed using Microsoft Excel (Office 365 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 0.625 units of Immolase™ Taq polymerase (Bioline, London, UK), 2 µL genomic DNA and PCR-grade H2O ...
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bioRxiv - Evolutionary Biology 2024Quote: ... dNTPs (0.2 mM each) (Bioline, London UK), MgCl2 (1.5 mM ...
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bioRxiv - Evolutionary Biology 2024Quote: ... MgCl2 (1.5 mM) (Bioline, London UK), 0.5 µM each of forward and reverse primers ...
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bioRxiv - Genomics 2024Quote: ... using 10 µL SensiFAST SYBR No-ROX mix (2x) (Bioline Inc., Taunton, MA), 300 nM forward and reverse primer ...
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bioRxiv - Cell Biology 2024Quote: ... Real time qPCR was performed using a RotorGene 6000 (Corbett Research) instrument with SYBR (Bioline, UK) and results were analysed using beta-actin (Actb ...
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bioRxiv - Microbiology 2024Quote: ... Single colonies were tested (n = 24-48) for the loss of the vector (erythromycin susceptibility) and by discriminatory PCR (MyTaq HS - Bioline) with specific oligonucleotides to select mutant over WT genotypes ...
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bioRxiv - Microbiology 2024Quote: ... or Accuzyme (Bioline). All plasmids are listed in Table S4.
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bioRxiv - Microbiology 2024Quote: ... The qPCR was performed as described (24) using 2x SensiFast mastermix (Bioline) with primers and probes to a final concentration in 25uL reaction volume of 0.32uM (primers ...
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... 500 ng of RNA was used for reverse transcription with a Sensifast cDNA synthesis kit (Bioline). RT-qPCR was performed with LightCycler 480 SYBR Green I Master reagent with the primers listed below.
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized utilizing a gene-specific primer designed to target the 10xSunTag socRNA and Tetro Reverse Transcriptase (Bioline). The resulting cDNA was isolated via column-based purification using the GeneJet Gel Extraction Kit (Thermo Scientific) ...