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Citations for Bioline :
1 - 50 of 1617 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... using the Sensifast SYBR no Rox kit (Bioline, BIO-98020). Data analysis and visualisation was performed using Microsoft Excel (Office 365 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 0.625 units of Immolase™ Taq polymerase (Bioline, London, UK), 2 µL genomic DNA and PCR-grade H2O ...
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bioRxiv - Genomics 2024Quote: ... in the presence of RiboSafe RNase Inhibitor (Bioline). Reverse transcription was conducted using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: ... RT-qPCR was conducted using SensiMix SYBR Hi-ROX 2x Master Mix (Bioline) on the Rotor-Gene Q (Qiagen) ...
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bioRxiv - Developmental Biology 2024Quote: Snap frozen cell pellets had total RNA extracted using ISOLATE II RNA mini kit (Bioline)
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bioRxiv - Evolutionary Biology 2024Quote: ... dNTPs (0.2 mM each) (Bioline, London UK), MgCl2 (1.5 mM ...
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bioRxiv - Evolutionary Biology 2024Quote: ... MgCl2 (1.5 mM) (Bioline, London UK), 0.5 µM each of forward and reverse primers ...
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bioRxiv - Genomics 2024Quote: ... using 10 µL SensiFAST SYBR No-ROX mix (2x) (Bioline Inc., Taunton, MA), 300 nM forward and reverse primer ...
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bioRxiv - Microbiology 2024Quote: ... Single colonies were tested (n = 24-48) for the loss of the vector (erythromycin susceptibility) and by discriminatory PCR (MyTaq HS - Bioline) with specific oligonucleotides to select mutant over WT genotypes ...
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bioRxiv - Microbiology 2024Quote: ... or Accuzyme (Bioline). All plasmids are listed in Table S4.
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bioRxiv - Immunology 2024Quote: ... Quantitative PCR was performed using 2X Taq based Master Mix (Bioline) and Taq Man gene expression assays (Applied Biosystems) ...
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bioRxiv - Immunology 2024Quote: Total RNA was isolated from mouse tissues using TRIsure reagent (BIO-38033, Bioline Gmbh, Germany) and genomic DNA was digested using RNase-free DNase (rDNase ...
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bioRxiv - Genetics 2024Quote: ... and 0.06 units of Velocity DNA Polymerase (Bioline, UK) and 50 ng of template DNA ...
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bioRxiv - Genetics 2024Quote: ... and 0.12 units of Velocity DNA Polymerase (Bioline, UK) and 0.5 μL of first round PCR product as template.
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bioRxiv - Plant Biology 2024Quote: ... 2.5 uL ImmoBuffer™ (Bioline) 103 ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was reverse transcribed with SensiFAST cDNA Synthesis Kit (Bioline) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2024Quote: ... 0.125 uL Immolase™ DNA polymerase (Bioline), 10 uL DNA for a total of 10ng input ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative Real-time PCR (qRT-PCR) was carried out using SensiFAST™ SYBR® No-ROX One-Step Kit (Bioline) and the results were analysed using the 2−ΔΔCt method (Livak & Schmittgen ...
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bioRxiv - Cell Biology 2024Quote: ... Genotyping PCRs were performed with MyTaq HS Red Mix (Bioline), using primers surrounding the genomic target sites ...
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bioRxiv - Physiology 2024Quote: ... using SensiFAST SYBR No-ROX Kit (Cat no. BIO-98050, Bioline, Froggabio, Canada). The amplification reaction contained 1 μL of diluted cDNA ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1mM dNTP Mix (Bioline), 40 units of Ribosafe RNAse Inhibitor (Bioline) ...
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bioRxiv - Plant Biology 2024Quote: ... The qRT-PCR amplification was performed with SensiMixTM SYBR NO-Rox Kit (Bioline) according to the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2024Quote: ... PCR was performed using MyTaq (Bioline) DNA Polymerase in reactions containing 30ng of DNA template ...
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bioRxiv - Synthetic Biology 2024Quote: ... The gel contained 1.95 g of agarose powder (Bioline, final concentration 1.3% w/v) in 150 mL of 0.5 x TBE buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using Random hexamers and Tetro Reverse Transcriptase (Bioline). Quantitative PCRs (qPCRs ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA from siRNA-treated cells was isolated using TRIsure (Bioline). Next ...
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bioRxiv - Plant Biology 2024Quote: ... Gels were prepared by dissolving 1.5g molecular grade agarose (Bioline Reagents Ltd., London, UK) in 150ml 0.5x Tris-borate EDTA (TBE ...
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bioRxiv - Physiology 2024Quote: ... RNA was then synthesized into cDNA Tetrokit (Bioline, UK) and subjected to qPCR analysis using SYBR green and LightCycler 480 (Roche) ...
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bioRxiv - Plant Biology 2024Quote: ... cDNAs were diluted to 50 µL and used as template for Real-Time PCR monitored by SensiFast SYBR No-Rox Kit (Bioline, Cincinatti, OH, USA). RT-PCR was performed in the Roche Light Cycler 480 using primers for CAH4 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were prepared for qPCR in technical triplicates in 10-µl reaction volumes using SensiFAST SYBR Lo-ROX 2X Master Mix (Bioline; Cat. No. BIO-94005), custom qPCR primers from Integrated DNA Technologies used at a final concentration of 0.2LµM and cDNA diluted at 1:3 ...
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bioRxiv - Neuroscience 2024Quote: ... Complementary DNA was synthesized with the SensiFAST cDNA Synthesis Kit (Bioline; Cat. No. 65054). Samples were prepared for qPCR in technical triplicates in 10-µl reaction volumes using SensiFAST SYBR Lo-ROX 2X Master Mix (Bioline ...
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bioRxiv - Evolutionary Biology 2024Quote: ... using the MasterMix qPCR No-ROX PyroTaq EvaGreen 5x for HRM (CMB-Bioline, Madrid, Spain). Two microliters of the diluted DNA sample were mixed with 0.3 μL (10 μM ...
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bioRxiv - Plant Biology 2024Quote: ... This template was then used to quantify relative mRNA abundance using the SensiMix SYBR Low-ROX kit (Bioline), a LightCycler® 480 (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: ... thermocycler using qPCR MasterMix No-ROX PyroTaq EvaGreen 5x for HRM (CMB-Bioline, Madrid, Spain). The PCR program consisted of an initial step of pre-incubation denaturation at 95°C for 15 min followed by 45 cycles of denaturation at 95°C for 15 s ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Quantitative analysis of the ERBB2 was performed using a SensiFAST™ SYBR Hi-ROX kit (Bioline, Canada). PCR amplification condition was applied as described previously [29] ...
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bioRxiv - Microbiology 2024Quote: ... 1 ng/μl of total DNA was amplified using the primer sets in SensiFAST SYBR No-ROX (Bioline). qPCR was realized in technical duplicates in the Rotor-Gene Q real-time PCR cycler (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Transcripts were quantified using SensiFAST SYBR (BioLine) on a LC480 instrument ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... We synthesized complementary DNA (cDNA) from 100 ng of RNA in a 40µl reaction containing 100 units of Tetro Reverse transcriptase (Bioline, Taunton, MA), 8µl of 5X RT buffer ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 40 units of Ribosafe RNAse Inhibitor (Bioline), and 4µl of 10X random primers (Applied Biosystems ...
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bioRxiv - Plant Biology 2024Quote: ... Quantitative PCR was carried out using 5x PyroTaq qPCR mix Plus EvaGreen (CMB Cultek Molecular Bioline) in a QuantStudio5 machine (Applied Biosystems) ...
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bioRxiv - Genetics 2024Quote: ... All genotyping PCRs were carried out using MyTaq™ Red Mix (Bioline, Cat# BIO-25043) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The qPCR was performed as described (24) using 2x SensiFast mastermix (Bioline) with primers and probes to a final concentration in 25uL reaction volume of 0.32uM (primers ...
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bioRxiv - Microbiology 2024Quote: ... 4.2 µL of extracted DNA was combined with 5 µL of MyTaq Red polymerase (Bioline, Meridian Bioscience), 0.4 µL of 10 uM forward primer (nexF-N3-6-515f for 16S or nexF-N3-6-ITS1f-KYO1 for ITS) ...
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bioRxiv - Molecular Biology 2024Quote: ... real-time PCR was conducted using the SensiFAST SYBR real-time PCR kit (Bioline). The obtained data were normalized to the human endogenous control (GAPDH ...
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bioRxiv - Molecular Biology 2024Quote: ... After selection on LB-agar kanamycin plates the resulting colonies were first screened by colony PCR using MyTaq DNA polymerase (Bioline) to check for the presence of the right size insert using T7forward and T7reverese primers ...
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bioRxiv - Molecular Biology 2024Quote: ... utilizing SensiFast Lo-ROX qRT-PCR Mastermix (Bioline) in both biological and technical triplicate ...
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bioRxiv - Molecular Biology 2024Quote: ... 2.5 µl of 1:10 diluted cDNA was added to 7.5 µl of Sensi-FAST SYBR No-ROX (Bioline) and 400 nM of forward and reverse primers in a 15 µl final volume ...
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bioRxiv - Molecular Biology 2024Quote: ... treated RNA was converted to cDNA using BioScript Reverse Transcriptase (Bioline) with (dN)6 random primers or dT18 primers as described in40 ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.25– 1 µg of RNA was reverse-transcribed into cDNA using the SensiFAST cDNA Synthesis Kit (Bioline, BIO-65054). Real-time PCR was performed on the CFX384 Touch real-time PCR detection system (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... we used the ISOLATE II RNA mini kit including on-column DNA digestion (Bioline, UK). The concentrations were measured by NanoDrop ND-1000 (Peqlab ...