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Citations for GE Life Sciences :
3601 - 3650 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The α-syn oligomers and free α-syn from the supernatant were separated using a Superdex 200 Increase 10/300 GL (GE Healthcare, 28990944) equilibrated with PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... 250 μl were applied to an Äkta pure chromatography system equipped with a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL, USA) and run at 6 °C ...
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bioRxiv - Microbiology 2019Quote: ... 75-90 μl of affinity-purified protein sample was loaded onto a Superdex 200 Increase 10/300 GL SEC column (GE Healthcare, Chicago, IL, USA) previously equilibrated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... diluted to 0.5 mL in GF150 buffer and further purified via size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare and Life Sciences) with GF150 buffer at 0.5 mL/min ...
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bioRxiv - Biochemistry 2019Quote: ... Excess dye was removed by size-exclusion chromatography using PD-10 desalting columns containing Sephadex G-25 resin according to the manufacturer’s instructions (GE Healthcare Life Sciences, Amersham, UK). Concentration of the conjugate and degree of labeling (DOL ...
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bioRxiv - Biochemistry 2020Quote: ... 2 mL fractions corresponding to ∼10 mL of the elution peak were pooled and applied to a 1 mL StrepTrap column (GE Healthcare, #28-9075-46) pre-equilibrated with Buffer N with 2 mM DTT ...
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bioRxiv - Immunology 2020Quote: HEK293T cells were grown at 37°C to approximately 80% confluence in 10 cm tissue culture dishes using DMEM with 5% Hyclone Fetal Clone II (GE Healthcare Lifesciences, Logan, UT) and 1X antibiotic and antimycotic (Gibco ...
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bioRxiv - Microbiology 2020Quote: The content of IgG monomers was assessed by FPLC gel filtration chromatography in a Superdex 200 10/300 GL column (GE Healthcare, Pharmacia; Stockholm, Sweden), using 0.15 mol/L NaCl ...
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bioRxiv - Biochemistry 2021Quote: Three microliters of S-D614G in 1.2 mg/ml was mixed with 1.2 equivalent of RBD-chAb45 and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted protein was further purified by size-exclusion chromatography (SEC) using a sizeexclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Biochemistry 2021Quote: ... and incubated at room temperature for 1 hr before being separated by SEC using a size-exclusion column (Superose 6 increase 10/300 GL; GE Healthcare, U. S. A.) in TN buffer ...
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bioRxiv - Biophysics 2021Quote: ... The SΔTM protein was then further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL, USA) (Fig ...
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bioRxiv - Biophysics 2021Quote: ... The eluted S proteins were further purified by size-exclusion chromatography (SEC) using a Superose 6 increase 10/300 GL column (GE Healthcare, U. S. A.) in TBS buffer (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Biochemistry 2020Quote: ... and 300 μl of the collected supernatant was directly injected onto a Superdex 200 HR 10/300 column (GE Healthcare Life Science, Cat# 17517501). To avoid any aggregation artefacts due to freezing and thawing of the samples ...
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bioRxiv - Immunology 2021Quote: ... and the homogeneity and the size of the antibodies were analyzed by using an analytical Superdex 200 10/300 column (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Biophysics 2020Quote: ... Purification was performed using size exclusion chromatography (SEC) on an Äkta purifier (FPLC) system with a Superdex200 10/300 column from (GE Healthcare Life Science; S200). We found that reconstitution ratios of 1:33 for ΔH4H5:DMPC and 1:50 for ΔH5:DMPC resulted in a single and relatively narrow symmetric peak ...
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bioRxiv - Neuroscience 2020Quote: ... the supernatant was subjected to serial purification steps using Superdex 200 Increase 10/300 G size-exclusion and Hitrap Q Sepharose Fast Flow anion-exchange columns (GE Healthcare, Pittsburgh, PA, USA). Purified a-syn was applied to High capacity endotoxin removal spin columns (Pierce ...
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bioRxiv - Plant Biology 2021Quote: ... Protein was separated on the 10 % SDS PAGE and transferred on the polyvinylidene difluoride (PVDF) membranes (Hybond™-P; GE Healthcare, Piscataway, NJ, USA) using the wet transfer method (1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... diluted to 0.5 mL in GF150 buffer and further purified via size exclusion chromatography on a Superose 6 Increase 10/300 GL column (GE Healthcare and Life Sciences) with GF150 buffer at 0.5 mL/min ...
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bioRxiv - Immunology 2022Quote: ... The protein was eluted with 250 mM imidazole in HBS after which the imidazole was removed using a HiPrep™ 26/10 Desalting column (GE Healthcare, Diegem, Belgium). The Avi-His6-tag was cleaved by caspase-3 (produced in-house ...
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bioRxiv - Biophysics 2022Quote: ... The sample was then injected in an Äkta Purifier System equipped with a Superdex Peptide 10/300 GL column (GE Healthcare, Chicago, IL, USA) equilibrated with ultrapure water ...
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bioRxiv - Biophysics 2022Quote: ... The caveolin-containing eluate was concentrated and further separated by size exclusion chromatography using a Superpose 6 Increase 10/300 GL column (GE Healthcare, Boston, MA, USA) in buffer containing 200 mM NaCl ...
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bioRxiv - Bioengineering 2022Quote: ... cultured cells were fixed with 4% PFA in PBS for 20 min at room temperature (RT) and permeabilized and blocked with 0.1% Triton X-100 and 10% fetal bovine (FBS; GE Healthcare Life Science, Marlborough, MA) in PBS for 30 min at RT ...
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bioRxiv - Physiology 2020Quote: ... U2OS-ERα cells were cultured in phenol-free αMEM medium supplemented with 10% HyCloneTM charcoal/dextran stripped FBS (GE Healthcare Life Sciences, Pittsburgh, PA), 1% antibiotic/antimycotic ...
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended in 10 ml FACS wash and filtered through a nylon filter before being overlayed onto 3 ml Ficoll (GE Healthcare, 17-1440-03). Cell/Ficoll mixture was centrifuged at 931g for 1 hour at room temperature with brakes off ...
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bioRxiv - Cell Biology 2019Quote: ... and 85% of this suspension (containing membrane-cytoskeleton fraction) was loaded onto a Superdex 200 10/300 Gel Filtration column (GE Healthcare Life Sciences, UK) equilibrated with 20 mM Tris pH 7.2 ...
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bioRxiv - Physiology 2020Quote: ... blood samples were drawn and participants received 75 MBq of 18F-FDG intravenously immediately followed PET/MR scan for 50±10 minutes (SIGNA PET/MR, GE Healthcare, Waukesha, WI, USA). Skin and room temperatures were monitored with surface temperature probes ...
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bioRxiv - Physiology 2019Quote: The optical density of cell suspensions was measured at a wavelength of 600 nm using an Ultrospec 10 spectrophotometer (GE Healthcare, Chicago, IL, USA). The cell dry weight (CDW ...
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bioRxiv - Biophysics 2020Quote: ... Pure tau was finally isolated via size exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare LifeSciences, Little Chalfont, U.K.) equilibrated with the aforementioned SSPE buffer ...
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bioRxiv - Microbiology 2021Quote: ... Primers were labelled using T4 PNK in buffer A and [γ-P32]-ATP (10 mCi/mL, 3,000 Ci/mmol) and purified using G-50 Microspin columns (GE Healthcare, #27-5330-01). Reverse transcription reactions were stopped by adding 40 μL of cold 0.3 M Na-acetate ...
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bioRxiv - Physiology 2020Quote: ... Examinations were carried out with a linear array i13L probe (10-14 MHz) using a Vivid 7 Dimension ultrasound system (GE Healthcare Ultrasound, Horten, Norway). The operator was blinded to the genotype of the mice ...
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bioRxiv - Synthetic Biology 2019Quote: ... between 5 and 10 mg protein ml−1) was pipetted into the measurement cell of a MicroCal ITC200 isothermal titration calorimetry instrument (GE Healthcare Life Sciences, USA). To avoid potential further unfolding of mutant protein we directly analysed them in imidazole-containing buffer A without previous dialysis ...
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bioRxiv - Biochemistry 2021Quote: ... reaction mixture was terminated by heating and limit-digested products were recovered by doing centrifugation (10000 × g) for 10 min and then applied to Toyopearl S40 resin containing column (GE Healthcare-XK 26/100) for purification ...
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bioRxiv - Immunology 2020Quote: ... and the size and purity of each antibody were determined by size exclusion chromatography using a Superdex 200 10/300 column (GE Healthcare, Chicago, IL, USA).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The pancreas tissue was washed three times in HBSS+CaCl2 and resuspended in Ham’s F-10 (#SH30025.01, HyClone, GE Healthcare Bio-sciences, Pittsburgh, PA, USA). Pancreas tissue was filtered through a 70 μm cell strainer and islets were handpicked under a dissecting scope to >95% purity.
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bioRxiv - Plant Biology 2022Quote: ... The fractions containing the protein were pooled and the imidazole removed on a PD-10 (sephadex G-25) column (GE Healthcare, Chicago, IL, USA), and the protein was concentrated in a centrifugal concentrator (Amicon MWCO 3 kDa ...
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bioRxiv - Microbiology 2022Quote: ... of this fraction was conducted using a Superdex® 200 Increase 10/300 GL on the ÄKTA pure protein purification system (GE Healthcare, Uppsala, Sweden). The proteins were eluted with PBS (pH 7.4 ...
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bioRxiv - Plant Biology 2022Quote: ... 3 mL of the centrifuged supernatant was applied to a PD-10 column filled with Sephadex G-25 resin (GE Healthcare, Chicago, IL, USA) equilibrated with the homogenizing buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (30 μg) were resolved by electrophoresis in a 10% or 12% gel and transferred to nitrocellulose membranes (GE Healthcare, Westborough, MA, #88018). The membranes were then incubated with specific antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... The concentrated sample was then subjected to size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), in buffer containing 150 mM NaCl ...
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bioRxiv - Bioengineering 2024Quote: ... The oligomeric state and purity were determined at 25°C by size-exclusion chromatography on a Superdex 200 10/300GL column (GE Healthcare Technologies, Chicago, IL).
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bioRxiv - Microbiology 2023Quote: ... The parasite were grown until late schizonts and layered over a 67% Percoll density gradient buffer in cRPMI (Percoll, 10 mM NaH2PO4, 143 mM NaCl (GE Healthcare Bio-Sciences, Sweden)) and centrifuged (1500 g / 15 mins ...
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bioRxiv - Molecular Biology 2023Quote: Mouse cortical and hippocampal brain homogenates were filtered through a cellulose acetate membrane (10 µg per dot) with a pore size of 0.2 µm (GE Healthcare Life Sciences, Munich, Germany). Per cavity of filtration unit ...
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bioRxiv - Cell Biology 2023Quote: ... estimated by Bradford assays) were separated in a 10 % (w/v) polyacrylamide gel and then transferred on PVDF membranes (GE Healthcare Life Sciences, Amersham). Immunodetection was performed with an anti-HA monoclonal antibody [HA Epitope Tag (YPYDVPDYA ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants from all 10 replicates of a species were pooled and mixed before filter sterilizing them with a 0.2 µm filter (GE Healthcare Life Sciences, Whatman filter) and aliquoting and storing them in 2 mL Eppendorf tubes at -20° C.
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bioRxiv - Cell Biology 2024Quote: ... Reduction of disulfide bonds and alkylation of cysteine residues was performed by incubating gel fragments in 10 mM 1,4-Dithio-D-threitol (GE Healthcare; cat. #17-1318-02) at 50°C for 1 hour followed by a light-protected incubation with 55 mM iodoacetamide (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... estimated by Bradford assays) were separated in a 10 % (w/v) polyacrylamide gel and were transferred on PVDF membranes (GE Healthcare Life Sciences Amersham). Immunodetection was performed with an anti-FLAG antibody (Agrisera ...
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bioRxiv - Bioengineering 2024Quote: ... eluted fractions from the affinity chromatography column were subjected to size exclusion chromatography (SEC) using an Enrich 70 10/300 column (GE Healthcare Biosciences, Uppsala, Sweden), connected to the NGC Chromatography System (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: Recombinant TopBP1 and GINS proteins were mixed in an equimolar ratio to give a final concentration of 20 µM of each component and incubated on ice for at least 30 minutes prior to application to a Superdex200increase 10 300 column (GE Healthcare, Little Chalfont, UK) equilibrated in 10 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... thawed ASK1 TBD-CRR-KD was subjected to size exclusion chromatography on a Superdex 200 10/300 GL column (GE Healthcare, Chicago, IL, USA) in a buffer containing 20 mM Tris-HCl (pH 7.5) ...