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Citations for GE Life Sciences :
3301 - 3350 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Peak fractions were pooled and further purified by gel filtration with a Superdex 200 increase 10/300 GL column (GE Healthcare). Peak fractions were pooled and concentrated to 11 mg/ml.
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... The cleaved protein was concentrated and loaded onto a Superose 6 Increase size exclusion chromatography column (10/300 prep grade, GE Healthcare). Elution was carried out with 150 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... The peak associated with pure ACBP was concentrated and further purified by SEC using a Superdex 75 (HR 10/30) column coupled to an Äkta purifier system (GE Healthcare) using Buffer A ...
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bioRxiv - Biochemistry 2023Quote: The ammonium sulfate treated Hsp10 preps were first diluted tenfold with chromatography buffer before loading into a HiPrep SP XL 16/10 column (GE Healthcare). A sodium chloride linear gradient from 0 to 1 M was used to purify Hsp10 and it eluted at a conductivity of 13.2 mS/cm ...
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bioRxiv - Biochemistry 2023Quote: ... with a 10 kDa MWCO before being subjected to size exclusion chromatography (SEC) on a Superdex 200 16/60 column (GE Healthcare) connected to an Äkta FPLC system (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing the protein of interest were concentrated and further purified by size-exclusion chromatography (Superdex 200 Increase 10/300, GE Healthcare) in a buffer containing 20 mM Tris-HCl ...
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... aVBD was then eluted in PBS and further subjected to purification using size exclusion chromatography using a Superdex 200 10/300 GL (Ge Healthcare). Samples were stored aliquoted at -80°C at concentrations ranging from 1 to 10 mg/ml.
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IpaA reveals distinct modes of vinculin activation during Shigella invasion and cell-matrix adhesionbioRxiv - Cell Biology 2023Quote: ... 200μl of the protein mixtures were analyzed by size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL (GE Healthcare) using a GE ÄKTA FPLC™ (Fast Protein Liquid Chromatograph ...
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bioRxiv - Biochemistry 2023Quote: ... and the phosphorylated kinase domain of FGFR1 was collected after size exclusion chromatography purification using Superdex 75 10/300 GL column (GE Healthcare). To prepare the dephosphorylated form ...
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bioRxiv - Biochemistry 2023Quote: ... the His6-NusA-tag was removed using the HRV3C protease and was further purified by SEC on Superdex 75 Increase 10/300 (GE Healthcare) column in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... with 500 mM NaCl in phosphate-buffered saline (PBS) on an Äkta Purifier 10 high-performance liquid chromatography system (GE Healthcare) to remove associated LPSs ...
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bioRxiv - Microbiology 2023Quote: ... The resulting supernatant was applied to a 10-mL column with 1 mL of Ni-Sepharose (GE Healthcare Life Sciences, 17531801) equilibrated with binding buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... A 2-fold molar excess of LF was mixed with 925 pmoles of hexon and incubated on ice for 5 min followed by SEC on Superose 6 increase 10/300 (GE Healthcare) equilibrated with buffer D ...
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bioRxiv - Biochemistry 2024Quote: apoGAD65 and b96.11 Fab were purified as described and complex formation performed by mixing freshly purified GAD65 with 2X molar excess of b96.11 followed by incubation on ice for 15 min before subjecting the complex to analytical size exclusion chromatography using a Superdex 200 Increase 10/300 GL (GE Healthcare). The sample was used as is for negative stain EM ...
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bioRxiv - Biochemistry 2024Quote: ... The N-terminal GFP-His10 tag was cleaved overnight with 3C protease before gel filtration chromatography by using a Sepharose 6 Increase 10/300 GL column (GE Healthcare). For the purification of apo proteins ...
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bioRxiv - Biochemistry 2024Quote: ... Bio-Beads were removed by centrifugation (250g, 10 min) and the supernatant was subjected to size-exclusion chromatography in a Superose 6 increase column (GE healthcare) equilibrated in SEC buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... The sample was then diluted with dilution buffer (50 mM HEPES (pH 7.2) and 5% glycerol) to reduce the salt concentration and loaded onto a Mono S 10/100 GL column (GE Healthcare). The column was washed with buffer C (50 mM HEPES (pH 7.2) ...
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bioRxiv - Immunology 2023Quote: ... The S proteins were then separated by chromatography using resin attached with streptavidin and further purified by size exclusion chromatography using a Superose 6 10/300(GE Healthcare) in 20 mM Tris ...
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bioRxiv - Systems Biology 2024Quote: ... SP3 magnetic beads were prepared as follows: per 10 samples 20 µL of each Sera-Mag Speed Beads A and B (GE Healthcare) were mixed 1:1 ...
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bioRxiv - Biophysics 2023Quote: ... developed with a salt gradient of 100 mM – 600 mM NaCl over 75ml followed by gel filtration on a Superdex 200 10/300 column (GE Healthcare) in buffer 20 mM HEPES ...
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bioRxiv - Biophysics 2024Quote: ... the ∼150 mM potassium phosphate buffer of the bPKA-C stock was exchanged to 10 mM potassium phosphate using a Hi-TRAP desalting column (GE Healthcare). Thus ...
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bioRxiv - Synthetic Biology 2024Quote: ... and those found to contain the purified protein were pooled and concentrated using a 10 kDa molecular weight cut-off Vivaspin spin column centrifugal concentrator (GE Healthcare) at 3894 x g and 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... was diluted to 50 μg/mL in 10 mM acetate buffer at pH 5.0 and immobilized on the surface of a CM5 sensor chip (GE Healthcare 29149604) using amine coupling chemistry ...
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bioRxiv - Biochemistry 2024Quote: ... The flow through was collected and further purified by size exclusion chromatography on the Superdex 200 Increase 10/300 GL (GE Healthcare). Peak proteins were pooled and verified by denaturing SDS PAGE ...
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bioRxiv - Neuroscience 2024Quote: ... Refolded and soluble proteins were concentrated using Vivaspin 20 or Amicon 15 centrifugal concentrators (10 kDa, 5 kDa, or 3 kDa molecular weight cutoff) and further purified on Superdex S75 columns (GE Healthcare) in SEC buffer (50 mM HEPES ...
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bioRxiv - Genomics 2024Quote: ... extension products were separated by electrophoresis on a 10% denaturing PAGE gel and visualized by a Typhoon Phosphorimager (GE Healthcare, Inc).
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bioRxiv - Biophysics 2024Quote: ... The column was washed with 40 column volumes of the same buffer (without MTSL) and further eluted with 300 mM imidazole and immediately deslated using a PD-10 desalting column (GE Healthcare) into the same buffer ...
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bioRxiv - Biophysics 2024Quote: ... Collected nanobody fractions were then fractionated by size exclusion chromatography (SEC) using a Superdex 75 Increase 10/300 GL column (GE Healthcare) in HBS buffer.
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bioRxiv - Biophysics 2024Quote: ... The protein was concentrated using a 100 kDa filter to approximately 1 mL and further purified by size-exclusion chromatography at 4°C using a Superose 6 10/300 GL column (GE Healthcare) equilibrated with buffer containing 0.06% digitonin ...
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bioRxiv - Biochemistry 2024Quote: ... Concentrated eluates were then applied to a 24-mL Superdex S200 increase 10/300 GL size exclusion column (GE Life Sciences). Fractions containing Cdc13 (Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... The membranes were washed with TBS-T three times for 10 minutes each and reacted with HRP-linked anti-rabbit or anti-mouse antibody (GE Healthcare) at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: Gel filtration was performed using concentrated IMAC purified proteins of 500µl in volume using a Superdex 75 GL column and Superdex-200 10/300 GL column preparative grade size exclusion chromatography column (GE Healthcare). The column was pre-equilibrated with SEC buffer composed of 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: Size-exclusion chromatography (SEC) was employed as the final purification step using a Superdex 200 Column (Superdex 200 Increase 10/300 GL, GE Healthcare) pre-equilibrated with three column volumes of SEC buffer (Tris-NaCl) ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was concentrated to 100 µL using a 100 kDa MWCO concentrator and the concentrate was loaded onto a size-exclusion chromatography (SEC) column (Superose 6 Increase 10/30 GL, GE Healthcare), equilibrated in a buffer composed of 20 mM Tris-Cl (pH 8) ...
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bioRxiv - Biochemistry 2024Quote: ... Here 70 µL of purified AaSiaP protein at 10 mg mL−1 was injected onto an inline Superdex S200 Increase 5/150 GL (GE Healthcare) SEC column ...
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bioRxiv - Biophysics 2024Quote: ... monomeric and tetrameric protein were separated and collected by SEC using a Superdex 75 Increase 10/300 GL column (GE Healthcare) and then used directly in fluorescence experiments (SI Appendix ...
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bioRxiv - Biochemistry 2024Quote: ... Target proteins in the HiTrap SP FF column were eluted by a linear gradient of NaCl and further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare). The purified proteins were of high purity (>95 % ...
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bioRxiv - Immunology 2024Quote: ... Complexes were generated by incubating Fab and antigen overnight at a 50:1 ratio and purified through size exclusion chromatography with a Superdex 200 increase 10/300 column (GE Healthcare).
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bioRxiv - Microbiology 2024Quote: ... and flow-through containing the Fabs was buffer exchanged to TBS using Vivaspin filters with a 10 kDa molecular weight cutoff (GE Healthcare).
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bioRxiv - Immunology 2024Quote: ... The S proteins were then separated by chromatography using resin attached with streptavidin and further purified by size exclusion chromatography using a Superose 6 10/300(GE Healthcare) in 20 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... or mixed protein and DNA were analysed by analytical gel filtration (a-SEC) using a Superdex 200 Increase 10/300 GL column (GE Healthcare) running in DNA binding buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... to get rid of the protease and further purified by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare). The purity of the recombinant protein was verified by SDS-PAGE and fractions with 90–95% purity were pooled and stored at –80°C.
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bioRxiv - Molecular Biology 2024Quote: ... Solid-phase syntheses of short oligonucleotides were performed in a 10 mL syringe equipped with frit and loaded with polystyrene support [ribo A 300 PrimerSupport 5G (299 μmol/g, GE Healthcare)] ...
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bioRxiv - Molecular Biology 2024Quote: ... This lysate was applied onto a 1 mL HisCap Smart 6FF column (Smart-Life Sciences) using an Akta Purifier 10 chromatograph (GE Healthcare). Subsequent washing steps were carried out with mix of buffer A and buffer B (buffer A + 500 mM imidazole) ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was desalted by a Zeba spin desalting column (7 kDa MWCO) and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare). Fluorescein- labeling efficiency was analyzed by SDS-PAGE and fluorescence gel imaging (Amersham Typhoon FLA 9500 ...
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bioRxiv - Molecular Biology 2024Quote: ... to get rid of the protease and further purified by size exclusion chromatography using a Superdex 200 10/300 GL column (GE Healthcare). The purity of the recombinant protein was verified by SDS-PAGE and fractions with 90–95% purity were pooled and stored at –80°C.
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bioRxiv - Microbiology 2024Quote: ... The fractions containing L–P protein were further purified using a size-exclusion column (Superose 6 Increase 10/300 GL, GE healthcare) equilibrated with size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2024Quote: ... The molecular weight of protein complexes was determined by size exclusion chromatography performed on Superdex 200 Increase 10/300 column (GE Healthcare) calibrated with High Molecular Weight calibration kit (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... The protein eluted from the HisTrap HP column was further purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Healthcare) equilibrated in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was then passed through an analytical gel filtration column called Superdex 200 Increase 10/300 GL (GE Healthcare, USA) with the running buffer (25 mM Tris-HCl ...