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Citations for GE Life Sciences :
3451 - 3500 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Transthoracic echocardiography was performed using a 10-14-MHz i13 probe and the Vivid 7 Ultrasound machine (GE Healthcare Ultrasound, Horten, Norway). Mice were lightly anesthetized with isoflurane ...
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bioRxiv - Cell Biology 2023Quote: ... Concentrated protein was further purified by size exclusion chromatography on a Superdex 200 Increase 10/300 GL (GE healthcare, GE28-9909-44) in SEC buffer ...
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bioRxiv - Biophysics 2023Quote: ... The amine modified dT nucleotides were labeled with Cy5 in a 20 µL reaction containing 10 mM (200 nmol) NHS-Cy5 (GE Healthcare, PA15100), 0.1 mM (2 nmol ...
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bioRxiv - Neuroscience 2023Quote: ... then centrifuged (400g for 10 minutes) and the resulting pellet was re-suspended in 30% Percoll (GE Healthcare BioScience AB, Uppsala, Sweden) and centrifuged at 500g for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The eluate fraction was dried using a vacuum centrifuge and resuspended in 3.6 mL ampholyte solution (pI 3-10, GE Healthcare, Chicago, IL). Offgel fractionation was performed using a 3100 OFFGEL fractionator (Agilent ...
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bioRxiv - Biochemistry 2023Quote: ... Protein samples at 12-25 mg/ml were prepared as described above for SEC-MALS and were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Hepes pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The fractions containing the desired protein were pooled together and concentrated to 2ml using a 10 kDa cutoff Vivaspin20 concentrator (GE Healthcare Biosciences). Then the protein was subjected to size-exclusion chromatography using HiLoad Superdex 200 prep-grade 16/600 column (GE Healthcare Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
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bioRxiv - Biochemistry 2023Quote: The changes in the oligomeric state of PrASNase were studied using the gel filtration column Superdex-200 (10/300 GL Increase, GE Healthcare, USA). The PrASNase was freshly purified and distributed equally in two different vials ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 2×106 CAFs were transfected with 3 nM of non-targeting control (D-001810-10-05, GE Healthcare Dharmacon, Inc., Lafayette, CO) or THY1 human siRNA (L-015337-00-0005 ...
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bioRxiv - Biophysics 2022Quote: ... The concentrated protein sample was subjected to further size-exclusion chromatography (SEC) by a Superose 6 Increase 10/300 GL gel filtration column (GE Healthcare, USA) using a flow rate of 0.3 mL/min and a running buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... then concentrated in a 10 kDa NMWL centrifugal filters (Amicon #UFC9003) to ∼500 μL and injected on a Superdex 200 10/30 GL size exclusion column (GE Healthcare #28990944) equilibrated in size exclusion buffer (SEB ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein lysates were run on 10% polyacrylamide SDS-PAGE gels and transferred to nitrocellulose membranes (Amersham Protran 0.45 µm NC - GE Healthcare Life Sciences). Membranes were blocked for 30 minutes in 5% milk in TBST buffer and incubated overnight with primary antibody (1:1000) ...
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bioRxiv - Biochemistry 2023Quote: ... 100 µL of purified proteins at 5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
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bioRxiv - Molecular Biology 2023Quote: ... the NbaAgo samples (3 mg/ml in 33 μl) were loaded onto a Superdex 200 Increase 10/300 column (GE Healthcare, USA) operated on a ProStar335 system and connected to a tandem of Prostar 335 detector (Varian ...
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bioRxiv - Bioengineering 2023Quote: ... 35 mM NaCl and 20 mM Tris HCl pH 8.3) and then purified by ion exchange using a Hi Prep Q FF 16/10 column (GE Healthcare Life Sciences) connected to an Akta Pure system (Cytiva) ...
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bioRxiv - Molecular Biology 2023Quote: ... 6xHis-3xFLAG-V1H(1-351) was further purified by gel filtration on a Superdex 200 10/300 GL column (GE Life Sciences) into the appropriate buffer for the subsequent application ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein extracts were resolved on SDS-PAGE (10% acrylamide) and transferred to a nitrocellulose Hybond ECL membrane (GE Healthcare, Pittsburgh, PA, USA) for probing with high-affinity rat monoclonal antibodies anti-HA (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was concentrated and subjected to gel-filtration chromatography using a Superdex 200 increase 10/300 column (GE Healthcare, USA), pre-equilibrated with gel-filtration buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... The processed proteins were purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Chicago, IL). Purified ...
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bioRxiv - Physiology 2023Quote: Plasma samples (200 μL) were loaded onto AKTA pure FPLC column [Superose™ 6 Increase 10/300 GL FPLC column (GE Healthcare). Lipoproteins were eluted with PBS and 0.25 mL fractions were collected at a flow rate of 0.4 mL/min.
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bioRxiv - Cancer Biology 2023Quote: ... to 20 mg/mL and purified by size exclusion chromatography on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences). Fractions containing pure Ras protein were pooled and concentrated to 20 mg/mL and stored at –78 ºC ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was diluted 1:10 v/v with 20 mM Tris 8.0 and further purified with anion exchange chromatography (HiTrapQ column, GE Healthcare Life Sciences) using a NaCl gradient of 50 mM to 500 mM in 20 mM Tris 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples at concentrations >5 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... This clarified sample was filtered (0.2 µm) and loaded onto a cation exchange chromatography SP FF 16/10 column (GE Healthcare Life Sciences) using the AKTA FPLC system ...
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bioRxiv - Pathology 2024Quote: ... Proteins were separated by SDS-PAGE (10 % polyacrylamide gel) and transferred to a PVDF membrane (RNP303F, GE Healthcare, Little Chalfont, Buckinghamshire, UK) in a semi-dry blotter (TE70X ...
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bioRxiv - Immunology 2024Quote: ... was incubated at room temperature for 20 minutes with a 1.2-fold molar excess of ADI-46143 Fab and the complex was purified by SEC on a Superdex 200 Increase 10/300 GL (GE Healthcare Biosciences) in 2 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... Complexes were incubated for 20 minutes at 23 ℃ before further purification via SEC on a Superdex 200 Increase 10/300 GL (GE Healthcare Biosciences) in 2 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was filtered with a 0.22 βM filter and loaded onto a tricorn 10/100 Capture Select C-tag XL™ resin (GE Healthcare, USA). The column was pre-equilibrated with 25 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: SEC coupled with MALS (SEC-MALS) was done using a Superdex® 75 10/300 GL column (GE Healthcare Life Sciences, USA) and a combination of a UV-Vis Prostar 335 detector (Varian ...
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bioRxiv - Molecular Biology 2024Quote: ... ALBA1 was further purified on a HiLoad Superdex™ 200 10/300 GL prep grade column (GE Healtcare) connected to an HPLC ÄKTA Purifier system (GE Healthcare). Eluates were monitored at A280 ...
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bioRxiv - Biochemistry 2024Quote: ... The concentrated and filtered sample was then finally passed through a HiLoad 10/300 Superdex 75 column (GE Healthcare, Chicago, IL, USA) equilibrated with SEC buffer [20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Bioengineering 2024Quote: ... beads were suspended in 0.22 mm filtered PBS with 5 ml (10 units) of PreScission protease (catalog # 27084301; GE healthcare Life Sciences) and incubated overnight at 4°C on a rotospin test tube rotator for on-column PreScission protease cleavage ...
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bioRxiv - Microbiology 2024Quote: ... Protein samples were concentrated using 10,000 MWCO spin columns to approximately 250 µL and injected onto a size exclusion column (Superdex 75 Increase 10/300 GL column, GE Life Sciences) preequilibrated with size exclusion buffer (25 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: Oligomerization of ClpL WT and mutants was investigated by size exclusion chromatography (SEC) using a Superose® 6 10/300 GL column (GE Healthcare) at 4°C in assay buffer supplemented with 2 mM ATP ...
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bioRxiv - Plant Biology 2020Quote: The enzymes’ buffer was changed to 10 mM MOPS (pH 7) using the PD Spintrap™ G-25 (GE Healthcare, 28-9180-04) protocol ...
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bioRxiv - Microbiology 2020Quote: ... of each peptide was serially diluted 2-fold in a 1:10 dilution of phosphate-buffered saline (PBS) (Hyclone; GE Healthcare, Pittsburgh, PA) in dH2O (10%PBS) ...
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bioRxiv - Molecular Biology 2019Quote: ... cell lysates corresponding to 2×105 cells in the case of in vivo experiments or 4×105 per sample in the case of nuclease-treated lysates were analyzed by electrophoresis on 10 or 12% SDS-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science). The proteins of interest were probed with antibodies against the following proteins ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 mM TCEP) using an AKTA pure chromatography system with a Superdex 200 10/300 GL column attached (GE Healthcare, GE17-5175-01). The in vitro interaction experiments using recombinant Ana1 fragments and commercial GST-PBD (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... The affinity tag for Munc18 was cleaved by 2 mg of TEV in PBS with 400 mM imidazole overnight at 4℃ and additional purification was performed using superdex 200 10/300 GL (GE Healthcare, Piscataway, NJ) gel filtration column in TBS buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The primary antibody solution was dumped and the membrane washed three times for 10 minutes each with 1xTBST before adding the polyclonal Anti-Rabbit IgG Peroxidase (GE Healthcare; NA9340V; 16908235) at a 1:5000 dilution in milk ...
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bioRxiv - Neuroscience 2021Quote: The SH-SY5Y cells were purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan) and maintained in MEM/F-12 mixture containing 10% fetal bovine serum (FBS) (HyClone; GE Healthcare Life Sciences) and 1% penicillin-streptomycin ...
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bioRxiv - Biophysics 2019Quote: ... expressed aSyn was purified using ion exchange chromatography (IEX) on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden). aSyn was then further purified on a HiPrep Phenyl FF 16/10 (High Sub ...
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bioRxiv - Biophysics 2019Quote: ... was used to remove the residual impurities by passing through a Superdex 200 10/300 GL gel filtration column (GE Healthcare Life Sciences). The peak fractions were collected and concentrated to 10-12 mg/ml ...
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bioRxiv - Biophysics 2019Quote: ... The fractions corresponding to pure DGRASP55/65 were then concentrated and applied into a Superdex200 10/300 GL gel filtration column (GE Healthcare Life Sciences) equilibrated in a 25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2019Quote: ... and HeLa cervical cancer cells were acquired from the American Type Culture Collection (ATCC) and cultured in phenol red-containing HyClone RPMI-1640 media with 10% (v/v)-heat-inactivated FBS (GE Healthcare Life Sciences) and 1% penicillin-streptomycin ...
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bioRxiv - Biophysics 2019Quote: ... and subsequently desalted and buffer exchanged (in to 20 mM phosphate buffer) using a HiPrep 26/10 Desalting column (GE Healthcare, Piscataway, NJ). The purity of affinity purified histidine tagged CsgF was investigated using SDS PAGE and the presence of the histidine tag was confirmed by western blot using an Anti-His (C-Term)-HRP antibody (Life Technologies ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Purified proteins were analyzed by size-exclusion chromatography on a Superdex 200 increase 10/300 GL column on an ÄKTA FPLC (GE Healthcare, Amersham Biosciences). SDS-PAGE was performed with 10% gels (Invitrogen ...
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bioRxiv - Biophysics 2019Quote: ... The mAb TS2/4-biotin was purified from an excess of NHS-LC-LC-Biotin via gel filtration (Superdex-200 10/300; GE Healthcare Life Sciences), concentrated using Amicon Ultra-4 centrifugal filters (10 kDa cut-off ...
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bioRxiv - Bioengineering 2019Quote: Purified proteins were analyzed by size-exclusion chromatography on a Superdex 200 increase 10/300 GL column on an ÄKTA FPLC (GE Healthcare, Amersham Biosciences). SDS-PAGE was performed with 10% gels (Invitrogen ...