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Citations for GE Life Sciences :
3551 - 3600 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The entire ectodomain of IFX was used as the analyte which was first purified and resolved by size exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare, Chicago, IL) in HBS-EP (10 mM HEPES ...
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bioRxiv - Biochemistry 2020Quote: ... and the CBP eluates were further purified by gel-filtration chromatography using a Superdex 200 Increase 10/300 GL column on an ÄKTA Pure purification system (Cytiva, formerly GE Healthcare Life Sciences). The proteins in the peak fractions were collected and concentrated to 1-3 mg/mL for storage at −75°C ...
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bioRxiv - Immunology 2020Quote: ... The PBMC was isolated within 10 min after blood collection by density gradient centrifugation over Ficoll-Paque Plus (GE Healthcare, Piscataway, NJ, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... The elimination of imidazole was achieved by gel filtration on columns filled with 10 ml of Sephadex G-25 (GE Healthcare Life Sciences) previously equilibrated with ice-cold PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The elution was concentrated and loaded onto a Superdex 200 Increase 10/300 GL size exclusion column (GE Healthcare Life Sciences, Pittsburgh, PA) equilibrated with minimal buffer (25 mM HEPES-NaOH (pH 7.5) ...
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bioRxiv - Physiology 2020Quote: ... and maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fischer Scientific, Waltham, MA) supplemented with 10% Fetal Bovine Serum (FBS) (GE Healthcare HyClone Laboratories, Logan, UT), non-essential amino acids (GE Healthcare HyClone Laboratories ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... of prepared protein samples were loaded and separated on 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membrane (GE Healthcare, Buckinghamshire, UK). NZYColour Protein Marker II (NZYTech ...
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bioRxiv - Biochemistry 2021Quote: ... concentration to 1-2 mL in 10 kDa MWCO spin concentrators and applied to either a HiLoad Superdex 75 pg 16/600 or a Superdex 75 Increase 10/300 GL SEC column (GE Healthcare, IL, USA) equilibrated in 20 mM Tris-HCl pH 7.4 and 150 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was purified away from SenP1 and the N-His6-SUMO-tag by FPLC on a Mono Q 10/100 GL anion exchange column (GE Healthcare Life Sciences) using buffers containing 50 mM EPPS (pH 8.5) ...
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bioRxiv - Biophysics 2020Quote: ... buffer twice and then subjected to size-exclusion chromatography on a 20 ml Superdex 200i 10/300 GL column (GE Healthcare Life Sciences) for detergent removal.
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bioRxiv - Immunology 2020Quote: Purified CoV-S trimers were concentrated to 1 mg/mL and loaded onto a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and passed through a Wyatt DAWN coupled to a Wyatt UT-rEX differential refractive index detector (Wyatt Technology) ...
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bioRxiv - Immunology 2020Quote: Purified C105 Fab was incubated with SARS-CoV-2 S trimer at a 2:1 molar ratio per protomer on ice for 30 minutes prior to purification by SEC on a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences). Fab–S complexes were concentrated to 1.6 mg/ml in Tris-buffered saline (TBS) ...
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bioRxiv - Cell Biology 2021Quote: ... Buffer was exchanged to BRB80 (80 mM NaPIPES, 1 mM MgCl2, 1 mM EGTA, pH 6.8) by loading protein over NAP- 10 (GE Healthcare, 17-0854-01) and PD-10 (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: ... dialysed into a Tris-saline buffer (Tris 10 mM, NaCl 150 mM, pH 7.4) then affinity purified on a protein A sepharose column (GE Healthcare, Chicago, IL, USA). Bound antibody was eluted with 0.1 M Glycine pH 2.4 and neutralised with 1 M Tris pH 8.5 ...
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bioRxiv - Neuroscience 2022Quote: ... see Figure 3 for detergent content) was added to 10 μl of packed beads: Protein G Sepharose 4 Fast Flow (GE Healthcare Life Sciences) for mouse antibodies or Protein A Sepharose 4 Fast Flow (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... were subjected to size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column connected to an AKTA PURE 25 FPLC system (GE Healthcare Life Sciences) maintained at 6° C ...
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bioRxiv - Biochemistry 2022Quote: ... SEC-HPLC was carried out using an Alliance e2695 HPLC system (Waters Co., Milford, MA, USA) equipped with a Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) and a 2998 photodiode array (PDA ...
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bioRxiv - Bioengineering 2022Quote: ... The 100 mM and 250 mM fractions containing the eluted endolysins were dialyzed against PBS at 4 °C and they were further purified using gel filtration (fast protein liquid chromatography) on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences). The final step was LPS removal ...
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bioRxiv - Plant Biology 2022Quote: ... Acetonenitrile was then added to 70 % and a 10:1 ratio of magnetic beads (Hydrophilic-Part no: 45152105050250, GE Healthcare plus Hydrophobic-Part no: 65152105050250, GE Healthcare, Australia) were added and mixed with the solution ...
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bioRxiv - Microbiology 2022Quote: ... brasiliensis by co-cultivation with yeasts at a ratio of 1:10 in sterile Hybond N filters (GE Healthcare Life Science, Pittsburgh, PA) in IM solid medium (induction medium ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were further purified by HiTrap Q HP ion-exchange chromatography and Superdex 200 Increase 10/300 GL gel filtration chromatography using an ÄKTApure chromatography system (GE Healthcare Life Sciences). Proteins corresponding to the molecular weight of the tetramerized Vip3Aa were used for subsequent biochemical and bioassay analyses ...
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bioRxiv - Immunology 2022Quote: ... Protein was concentrated in 10 kDa molecular weight cutoff centrifugal concentrators and purified by size exclusion chromatography on an AKTAPURE FPLC (GE Healthcare, Chicago IL), using an S200 column followed by a S75 column ...
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bioRxiv - Immunology 2023Quote: ... The EROS-NOX2-p22phox-7G5 Fab complex was concentrated and applied onto a Superose 6 Increase 10/300 GL column (GE HealthCare, Chicago, IL) in Buffer D (20 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... were incubated (18 min shaking at 1400 rpm) with 10 µL of equal volumes of hydrophilic (Speedbead magnetic carboxylated modified particles, GE Healthcare, United Kingdom) and hydrophobic (Sera-Mag Speedbead carboxylated-modified particles ...
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bioRxiv - Biochemistry 2024Quote: ... samples were loaded onto a Superose 6 10/300 gel filtration column under the control of an ÄKTA Pure instrument (GE Healthcare Life Sciences). Proteins were eluted using 50 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The ultracentrifuged sample was purified by size-exclusion chromatography on a Superose™ 6 Increase 10/300 GL column (GE Healthcare Life Sciences), equilibrated with buffer containing 20CmM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... Free Alexa Fluor 555 was then separated from RepA-Titinx-Alexa Fluor 555 using a HiPrep 26/10 desalting column (GE Healthcare, Piscataway, NJ). The labeled protein sample was dialyzed into storage buffer (40 mM Tris ...
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bioRxiv - Biophysics 2024Quote: All size exclusion chromatography was carried out using HMK buffer with 5 mM DTT added unless stated otherwise using a Superdex 200 10/300 column (GE healthcare Life Sciences). For each experiment ...
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bioRxiv - Biochemistry 2023Quote: ... The 5 mL sample concentrated from the fractions involving the target proteins with Amicon Ultra-15 10 kDa was loaded on Resource Q (GE Healthcare Life Science) anion-exchange column equilibrated with buffer A using the FPLC systems ...
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bioRxiv - Biophysics 2024Quote: ... The flow-through was further purified by size-exclusion chromatography (Superdex 200 Increase 10/300 GL size exclusion column GE Healthcare Life Sciences) in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Biophysics 2024Quote: ... Germany) and gel filtration fast protein liquid chromatography (FPLC) was performed on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences). The sample was dialyzed against phosphate-buffered saline (PBS ...
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bioRxiv - Biophysics 2023Quote: ... The digested protein was concentrated using a 100 kDa concentrator and further purified on a Superose 6 Increase 10/300 gel filtration column (GE Healthcare Life Sciences) equilibrated in an Mg2+-containing buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: Both mFE- and tFE-labeled proteins were further purified by size exclusion chromatography (SEC) using a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) in a buffer containing 20 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: The purity of the proteins and their aggregate formation or lack thereof were assessed using a Superdex™ S200 10/300GL SEC column (Cytiva, Vancouver, Canada) and an ÄKTA FPLC™ system (GE Healthcare) in HBS-EP buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed 3 times 10 minutes each with TBS-T and then incubated with the corresponding IgG secondary antibodies horseradish peroxidase-conjugated (GE Healthcare Life Sciences) at 1:1000 for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Samples previously centrifuged and filtered were run in a Superdex 75 Increase 10/300 GL column connected to an Äkta Pure System (GE Healthcare Life Sciences) equilibrated with buffer 10 mM sodium phosphate ...
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bioRxiv - Biochemistry 2023Quote: ... concentrated and further purified by size-exclusion chromatography in purification buffer including 10 mM imidazole using the column HiLoad® 16/600 Superdex® 75 prep grade (GE healthcare). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... and digested using the Macs Neural tissue Dissociation kit (Miltenyl Biotec) and subsequent percoll gradients (30% percoll [GE healthcare], 10% fetal bovine serum) was used to purify myeloid cells and incubated to antibodies against Cd11b and CD45 ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed three times with PBS for 10 minutes and subjected to signal detection using ECL™ Prime Western Blotting System (Cytiva) and ImageQuant™ LAS 4000 mini (GE HealthCare). The primary and secondary antibodies were diluted with a PBS solution containing 1% non-fat milk.
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bioRxiv - Cancer Biology 2024Quote: ... LLC2 cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% heat-inactivated fetal bovine serum (GE Healthcare Life Sciences, HyClone Laboratories) and 1% penicillin and streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... on an Akta Pure chromatography system using a Tricorn 10/300 column with Sepharose 4 Fast Flow resin (all GE Healthcare Life Sciences). EVs were then sterilized by 0.45 μm syringe-filtration ...
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bioRxiv - Cancer Biology 2024Quote: ... A549-luc cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum (GE Healthcare Life Sciences, HyClone Laboratories) and 1% penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... CezanneCAT was eluted by cleavage of the GST tag with 15 units/ml TEV protease (SigmaT4455-1KU) in PBS buffer overnight at 4°C and desalted using a PD-10 column (GE Healthcare, cat. # 17085101) in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The dye-labeled protein was purified from unreacted free dye using a PD-10 column containing Sephadex G-25 Medium (GE Healthcare Life Sciences) and then centrifuged at 50,000 rpm for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... University of Pennsylvania School of Medicine) [61] and HuR (19F12, Thermo) 400 μL of 10% (v/v) protein A Sepharose CL-4B beads (GE Healthcare Life Sciences) in IP-2 buffer [50 Mm Tris pH 7.9 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1.5 mg of HisTrap-purified NUCKS1 was diluted to 200 μl in storage buffer and loaded onto a Superdex 200 HR 10/30 column (GE Healthcare, Little Chalfont, UK), and 0.5 ml fractions collected.
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bioRxiv - Biophysics 2020Quote: ... Undigested IgG was removed from the quenched reactions by passing the reactions over a Superdex200 Increase 10/300 column (GE Life Sciences, Cat. #28990944) in tris-buffered saline.
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... Proteins (20-30 μg) were separated on 10% SDS-PAGE gels and blotted to a 0.45-μm pore nitrocellulose filter (GE Healthcare, Europe GmbH, Freiburg, Germany) using Trans Blot Turbo system (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Chemiluminescence detection was performed using the ECL Prime Western Blotting kit (GE-healthcare, RPN2232) and developed on a ImageQuant LAS 4000 (GE healthcare, 28-9558-10). Secondary antibodies for infrared fluorescence detection were donkey anti-rabbit-IRDye® 800CW (Rockland ...
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bioRxiv - Immunology 2022Quote: ... 50 mM imidazole) before eluting the protein (HBS, 250 mM imidazole) following desalting using a HiPrep™ 26/10 Desalting column (GE Healthcare, Diegem, Belgium) to remove imidazole ...