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Citations for GE Life Sciences :
3651 - 3691 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Lysates measured at 50 μg were separated using 10% sodium dodecyl sulfate-polyacrylamide electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride membrane (10600023; GE Healthcare Life Sciences, Chalfont, UK). After blocking with 5% skim milk (232100 ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1 to 2 mL culture volume at a determined OD730 (between 8 and 10) were filtered onto pre-dried and pre-weighed glass microfiber filters (47 mm diameter, 1 µm pore size, GE Healthcare, Cat. No. 1822-047), washed twice with deionized water and dried overnight at 65 °C (until mass deviation between readings was no higher than 0.0001 g) ...
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bioRxiv - Systems Biology 2019Quote: ... using a 5-ml HiTrap Chelating HP column charged with Ni2+ ions followed by buffer-exchange chromatography on a HiPrep 26/10 desalting column (both GE Healthcare Life Sciences, Pittsburgh, PA) using an ÄKTAxpress system (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2020Quote: HESCs were grown in a six-well culture plate to 60%–70% confluence and transfected with 60 pmol of non-targeting siRNA (D-001810-10-05) or siRNAs targeting ACE2 (L-005755-00-0005) (GE Healthcare Dharmacon Inc., Lafayette, CO) in Lipofectamine 2000 reagent (Invitrogen Corporation ...
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bioRxiv - Bioengineering 2020Quote: ... at room temperature for 6 h after which the excess crosslinker was removed with a disposable PD-10 desalting column (GE Healthcare Bio-Sciences, PA, USA). DBCO-functionalized nanobody was further refined on the fast-protein liquid chromatography (FPLC ...
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bioRxiv - Systems Biology 2021Quote: ... The lysate was boiled in Laemmli SDS-PAGE sample buffer for five minutes and 50 µg of protein per lane were separated onto 10% SDS-PAGE gels and transferred onto nitrocellulose membranes (GE Healthcare Life Science, Cat# 10600001). Following Ponceau S staining ...
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bioRxiv - Biophysics 2021Quote: ... on a gravity flow column followed by size-exclusion chromatography on a Superdex® 200 Increase 10/300 GL column (GE Healthcare Life Sciences, USA) in a buffer containing 10 mM NaCl and 10 mM sodium phosphate ...
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bioRxiv - Biophysics 2022Quote: ... TIA1 LC domain was isolated from the supernatant using a Bio-Rad NGC Discover 10 chromatography system and a 5 mL Ni-affinity column (Bio-Rad Bio-scale Mini Nuvia IMAC or GE Healthcare HisTrap FF crude). The column was equilibrated in 500 mM sodium chloride ...
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bioRxiv - Microbiology 2020Quote: ... DksA was exchanged into a buffer containing 15 mM sodium phosphate and 100 mM NaCl (pH 7) over a PD-10 Sephadex column (GE Healthcare Bio-Sciences, Pittsburg, PA). DksA protein solutions (12.5 μM ...
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bioRxiv - Microbiology 2019Quote: ... The gel was exposed for 10 min to a storage phosphor screen and visualized using a Typhoon FLA 7000 phosphor imager (GE Healthcare Bio-Sciences, PA, USA). For densitometric analysis ...
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bioRxiv - Biochemistry 2020Quote: ... Flow-through and washes were pooled and concentrated for size-exclusion chromatography over a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences, Marlborough, MA) that was equilibrated in buffer A (100 mM NaCl and 20 mM HEPES pH 7.5).
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bioRxiv - Microbiology 2020Quote: ... Enrichments were performed in test tubes containing 5 mL of M63 minimal media and a 1 x 10 cm strip of Whatman #1 cellulose filter paper (GE Healthcare Life Sciences, Pittsburgh, PA) pressed against the side of the tube (30) ...
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bioRxiv - Biochemistry 2022Quote: Verification of the monomeric or dimeric state of those constructs was obtained by SEC-MALS experiments with the injection of 5 mg/mL of each protein into a Superdex 200 10/30 Increase column (GE Life Sciences, Velizy-Villacoublay, France) mounted on a HPLC Ultimate 3000 (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was centrifuged at 10,000 xg for 20 min and the His6-tagged-proteins were purified by Ni-NTA chromatography followed by size exclusion gel filtration using Superdex 200 10/300 GL column (GE Healthcare Life Sciences, Pittsburg, PA).
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bioRxiv - Immunology 2024Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...
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bioRxiv - Immunology 2023Quote: ... Tissue was centrifuged at 500g for 5 minutes and resuspended in 10 mL of 30% Percoll solution (9 mL Percoll (GE Healthcare Biosciences, #17-5445-01), 3 mL 10X PBS ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were injected and purified on SEC (size exclusion chromatography) column Superdex 200 10/300 GL (GE Healthcare Life Sciences, Piscataway, NJ, USA) against a buffer containing 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were loaded into a 125 µl sample loop and injected onto a Superdex 75 10/300 GL column (GE Healthcare Life Sciences, Pittsburg, PA) equilibrated in MBS ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were resuspended in the medium for Leydig cells (low glucose DMEM (PanEco) with 10% fetal bovine serum (FBS, HyClone, GE Healthcare Life Sciences, Chicago, IL), 1% penicillin/streptomycin (PanEco) ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was concentrated and subjected to size-exclusion chromatography (SEC) on Superdex® 200 Increase 10/300 GL column (GE Healthcare Life Sciences, USA) in a buffer containing 150 mM NaCl and 25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: Shh size distribution in the presence or absence of soluble carriers was confirmed by SEC analysis with a Superdex200 10/300 GL column (GE Healthcare, Chalfornt St. Giles, UK) equilibrated with PBS at 4°C fast protein liquid chromatography (Äkta Protein Purifier (GE Healthcare)) ...
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bioRxiv - Neuroscience 2024Quote: Pooled soluble brain extracts were injected at 0.5 ml/min through a Superdex 75 (10/300) HPLC column (GE Healthcare Life Sciences, Pittsburg PA, USA) for 50 min and 0.25 ml fractions were collected ...
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bioRxiv - Molecular Biology 2024Quote: ... Tetrahydrofuran was removed under reduced pressure and the sample was desalted by size-exclusion column chromatography (GE Healthcare, HiPrep™ 26/10 Desalting; Sephadex G25) eluting with H2O ...
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bioRxiv - Synthetic Biology 2021Quote: ... membranes were washed three times with TBST for 10 minutes each and exposed to donkey anti-rabbit secondary antibody conjugated to horse radish peroxidase (GE Healthcare; NA934V; LOT: 16976257; 1:10,000) in TBST containing 5% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... Samples (100 µL OXAVL02 or OXAVL06 at 0.5-1 mg/mL) were loaded onto a Superdex® 200 Increase 10/300 G column (GE Healthcare Bio-Sciences AB Uppsala, SW) pre-equilibrated with the “SECMALS-PBS buffer” ...
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bioRxiv - Microbiology 2020Quote: ... A second fractionation step was performed with 55% to 80% ammonium sulfate fractions loaded on a PBS-equilibrated size exclusion column (Superdex® 200 Increase 10/300 GL, GE Healthcare, Little Chalfont, United Kingdom). The protein content was eluted in 0.5 mL fractions with one column volume of PBS ...
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bioRxiv - Biochemistry 2020Quote: Microsomal protein (2 µg) was separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences, Little Chalfont, UK). The membranes were blocked with 5% skim milk (BD Biosciences ...
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bioRxiv - Biochemistry 2020Quote: ... The redissolved protein sample was desalted using a NAP10 desalting column pre-packed with Sephadex G-25 resin (NAP™-10 Columns, Sephadex™ G25, GE Healthcare Life Sciences, UK) and eluted in 20 mM sodium phosphate buffer (pH 6.0) ...
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bioRxiv - Cell Biology 2023Quote: ... as a model of pre-adipocytes [30] were cultured in DMEM (Nacalai, Osaka, Japan) supplemented with 10% fetal bovine serum (HyClone; GE Healthcare, Salt Lake City, UT, USA) and a mixture of 100 units/ml penicillin and 100 μg/ml streptomycin (Nacalai ...
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bioRxiv - Neuroscience 2023Quote: ... This fraction was then further purified by size exclusion chromatography using the Superdex® 200 Increase 10/300 GL column (GE Life Sciences, 28-9909-44) equilibrated with 1x PBS supplemented with 137 mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... Separation of aSYN monomers and PFFs was performed with fast protein liquid chromatography using a Superose 6 10/300GL column (GE Healthcare, Life Sciences, Wauwatosa, WI, USA). PFFs were briefly sonicated ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times for 10 min in TBS-T and incubated with the secondary horse radish peroxidase (HRP) coupled antibody (GE Healthcare, NXA931V; in a 1:3000 dilution) for two hours under agitation at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... PDI (in PBS, pH 7) was submitted to size exclusion chromatography on a Superose™ 12 10/300 GL column (GE Healthcare, Cat. No. 17-5173-01) at a flow rate of 0.5 ml/min with PDI eluting between 11 and 14 ml.
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bioRxiv - Microbiology 2021Quote: ... Sample was concentrated with a 10 kDa NMWL spin concentrator and passed over a HiLoad® 16/600 Superdex® 200 pg (GE Healthcare, Cat. # 28-9893-35) size-exclusion (SEC ...
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bioRxiv - Zoology 2020Quote: ... The protein pellet was redissolved in 260 μl lysis buffer (6 M urea, 2 M thiourea, 4% CHAPS, 30 mM DTT, and GE Healthcare 2% IPG buffer pH 3–10). GE Healthcare IEF strips (pH 3–10NL ...
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bioRxiv - Cell Biology 2024Quote: Cell or collagen lysates were separated by denaturing SDS-PAGE (6% gels for collagens, 10% for SMADs) and proteins blotted onto a nitrocellulose membrane (Amersham™, GE Healthcare, Chalfont St Giles, United Kingdom). Membranes were cut horizontally to detect proteins of different sizes ...
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bioRxiv - Molecular Biology 2023Quote: For Western blot analysis Neurospora whole cell lysate was analyzed by SDS/PAGE and transferred to a nitrocellulose membrane (GE Healthcare; 0.45 μm, 10 Å∼ 150 cm). The membrane stained with Ponceau S to control for gel loading prior to antibody decoration.
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bioRxiv - Cell Biology 2023Quote: ... using a series of chromatography steps (XK 50/30 cationic exchange column [GE Healthcare] packed with SP-Sepharose Fast Flow [GE Healthcare], MonoQ HR 16/10 anionic exchange column [GE Healthcare] and TSKgel G4000SWxl column). SNAP-BICD2N and BICD2N-GFP (both containing residues 1-400 of mouse BICD2 ...
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bioRxiv - Biophysics 2019Quote: ... to maximum of 10 mg/ml and further purified by size exclusion chromatography on a Superdex 200 HiLoad column (GE Healthcare; PfELC, MTIP, MTIP-S, MLC1, MLC1-S) or on a Superdex 75 HiLoad column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... in 0.5X TBE/10% glycerol for 30 min with gentle agitation and then the fluorescence was imaged with a Typhoon Trio phosphoimager (GE Healthcare; 488 nm excitation and 526 nm emission). Assays were performed in duplicate with representative gels shown ...
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bioRxiv - Molecular Biology 2020Quote: ... Soluble chromatin was pre-cleared by incubation with 10 μL of 50% RIPA-equilibrated Protein A + G sepharose bead slurry (GE Healthcare, Cat. No 17-5280-11 and 17-0618-06) for every 100 μL of chromatin for 1 h at 4°C ...