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Citations for GE Life Sciences :
3401 - 3450 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The purified S2~UBC9 was concentrated and subjected to size-exclusion chromatography (SEC) with a Superdex 75 10/300 GL column (GE Life Sciences) on an ÄKTA explorer fast protein liquid chromatography (FPLC ...
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bioRxiv - Microbiology 2022Quote: ... HeLa and HEK293T/17 cells lines were purchased from ATCC (Manassas, VA) and cultured in Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal clone III (FC3; GE Healthcare Lifesciences, UT) and 1% Penicillin-Streptomycin (Pen Strep ...
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bioRxiv - Biophysics 2022Quote: ... coli upec-117 CapW at 5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in buffer GF ...
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bioRxiv - Biochemistry 2022Quote: ... Protein samples at concentrations >5 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2022Quote: Purified protein samples at a concentration of 0.5 mg ml-1 were injected onto a Superdex Increase 10/300 GL column (GE Healthcare, Diegem, Belgium) that was pre-equilibrated with HBS ...
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bioRxiv - Immunology 2022Quote: ... After being further purified by size-exclusion chromatography by Superdex 200 Increase 10/300 GL column (GE Healthcare cat.# GE28-9909-44), the protein was pooled and concentrated again for further use.
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bioRxiv - Biochemistry 2020Quote: ... 10% glycerol at pH 8.2) and loaded onto a 5 mL prepacked Q-sepharose ion exchange column (GE Healthcare Life Sciences, MA) at 1 mL/min ...
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bioRxiv - Bioengineering 2021Quote: Several DSAs were performed to guide the intervention by injecting approximately 5-10 ml of a half-strength contrast agent (CA) (50% of Omnipaque 240 (GE Healthcare, USA) and 50% of a saline solution ...
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bioRxiv - Plant Biology 2021Quote: ... and fractionated by gel permeation chromatography (GPC) using a Superdex 200 10/300 GL column (GE Healthcare, catalog no. 17-5175-01) connected to an AKTA FPLC system (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... Debris was pelleted for 10 min at 12000 x g and the supernatant incubated with PBS-washed glutathionesepharose 4FastFlow beads (GE Healthcare, 17075601) overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions were pooled and buffer-exchanged to sodium carbonate/hydrogen carbonate buffer pH 10 using a Sephadex G-25 column (GE Healthcare, UK). Recombinant protein was stored at −20 °C until required ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated protein was further purified using size-exclusion chromatography (SEC) on a Superdex 200 10/300 GL (GE Healthcare, Chicago, IL, USA) column containing 0.003% (w/v ...
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bioRxiv - Biochemistry 2019Quote: ... 100 μL of purified protein/complex at 2-5 mg/mL was injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in a buffer containing 20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... The sample (1 ml of protein with a concentration of 0.5 mg ml-1) was loaded onto a Superdex™ 75 Increase 10/300 GL (GE Healthcare Lifesciences) column at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Strep Tactin eluted protein was further purified by size exclusion chromatography using a Superdex 200 Increase 10/300 column (GE Life Sciences) in 25 mM HEPES ...
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bioRxiv - Plant Biology 2020Quote: ... Reconstituted proteins were separated from the non-reconstituted ones utilizing the size-based column chromatography technique (Sephadex G-25M columns (PD-10 column, GE Healthcare, USA) (for detailed procedure see Roell et al. ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Free proteins were labeled following the manufacturer’s instructions and purified using Sephadex G-25 in PD-10 Desalting Columns (GE Healthcare Life Sciences).
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bioRxiv - Plant Biology 2019Quote: ... An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL, GE Healthcare, www.gehealthcare.com) of an Äkta Avant System (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... Nsp12 eluates within conductivity of 19-23 mS/cm were combined and injected onto a Superdex 200 increase 10/300 GL column (GE Healthcare, USA) in a buffer containing 25 mM HEPES pH 7.5 ...
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bioRxiv - Zoology 2021Quote: ... NSF was further purified from the supernatant by size-exclusion chromatography on a Superdex 200 10/300 GL increase column (GE Healthcare, USA) with 10 mM phosphate buffer (Na2HPO4 ...
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bioRxiv - Biochemistry 2021Quote: ... The fractions near the maximum height of the peak were combined and further purified by a Mono Q 10/100 GL column (GE Healthcare, USA). Buffer exchange to buffer E ...
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bioRxiv - Cell Biology 2020Quote: ... the His6-tags were cleaved by thrombin digestion and a final size exclusion chromatography purification step using a Superdex 200 10/300 GL column linked with an AKTA FPLC (GE Healthcare, Inc.) was performed to achieve a purity of > 95%.
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bioRxiv - Molecular Biology 2021Quote: ... was transferred from the purification buffer into the labeling buffer (Na-PO4 20 mM, pH 8.3) supplemented with OG (30 mM) using PD-10 columns (GE Healthcare, Solingen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... SecYEG-loaded and empty nanodiscs were separated via size exclusion chromatography using Superdex 200 10/300 Increase GL column (Cytiva/GE Life Sciences).
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bioRxiv - Immunology 2020Quote: ... Complex samples were either diluted to 0.02 mg/mL and applied to glow discharged negative stain grids or they were SEC purified using a Superose™ 6 Increase 10/300 GL (GE Healthcare) column to remove excess Fab prior to EM grid preparation ...
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bioRxiv - Immunology 2020Quote: ... Clarified Expi293F culture supernatants were filtered and Fabs were purified by successive affinity and size-exclusion chromatography (on HiTrap Protein G and Superdex 200 Increase 10/300 GL columns, respectively, from GE Life Sciences) using an AKTA pure system.
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bioRxiv - Biochemistry 2021Quote: Protein complexes were separated according to relative molecular mass using an Äkta™ FPLC system with a Superdex 200 HR 10/30 column (GE Healthcare) and 24 × 106 cardiomyocytes per sample ...
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bioRxiv - Biochemistry 2021Quote: ... SEC-MALS runs were performed on an Agilent 1260 Infinity II Prime Bio LC System coupled with a Wyatt miniDAWN® MALS detector using a Superose6 increase 10/300 chromatography column (GE Healthcare) equilibrated with 25 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... The eluent was concentrated and further purified by using a size-exclusion chromatography (SEC) column (Superose 6 increase 10/300 GL, GE Healthcare, USA) in 50 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2020Quote: ... The next day the protein was dialyzed for an additional 3 hours in fresh storage buffer and subsequently applied to a Superdex 200 10/300 GL (GE Life Sciences) equilibrated in the same buffer ...
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bioRxiv - Synthetic Biology 2021Quote: ... Deutschland) size exclusion chromatography followed by a Superdex 200 Increase 10/300 GL column (ÄKTA Pure system, GE Healthcare, Boston, Massachusetts, USA) purification at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was centrifuged in a Beckman 45Ti rotor at 100,000 x g for 30 min at 4 °C and the supernatant applied to a HiLoad 26/10 Q Sepharose column (GE Life Sciences) equilibrated in 90 % buffer A and 10% buffer B (buffer A ...
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bioRxiv - Immunology 2021Quote: The protein was purified from filtered cell supernatants using Strep-Tactin resin (IBA) before being subjected to additional purification by gel filtration chromatography using a Superose 6 10/300 column (GE Healthcare, USA) in 1 × PBS ...
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bioRxiv - Immunology 2020Quote: ... elutions were further purified after affinity purification on an AKTA™ using a GE Superdex 200 Increase 10/300 GL column (GE HealthCare) in 1X PBS ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μL purified proteins at 2-5 mg/mL were injected onto a Superdex 200 Increase 10/300 GL column (GE Life Sciences) in gel filtration buffer ...
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bioRxiv - Biophysics 2022Quote: ... Sample homogeneity was investigated by analytical size exclusion chromatography on a Superose® 6 10/300 GL gel filtration column (GE Healthcare). Aliquots of purified EFL at 0.4 mg/mL were flash frozen in liquid nitrogen and stored at −70 °C.
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bioRxiv - Biochemistry 2022Quote: Cell lysates or mitochondria samples were separated on 10 – 18 % Tris-Tricine gels and transferred onto Amersham™ Protran™ 0.2 µM nitrocellulose membranes (NC, GE Healthcare). For immunodetection ...
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bioRxiv - Cell Biology 2022Quote: ... 300 μl plasma from WT and KO mice after 6 months of WD feeding were analyzed by FPLC column [Superose™ 6 Increase 10/300 GL FPLC column (GE Healthcare)] ...
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bioRxiv - Plant Biology 2022Quote: Total protein was extracted and subjected to fast-performance liquid chromatography (FPLC) using a Superdex Increase 200 10/300GL column (10246670, GE Healthcare, Sweden) to collect 1 mL fractions per sample ...
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bioRxiv - Biochemistry 2022Quote: ... The complex formed in the 1:2.5 mixture was purified on a Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in assay buffer and run at room temperature ...
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bioRxiv - Microbiology 2023Quote: 0.5 mg of the Ni-NTA protein eluate was analyzed by size exclusion chromatography (SEC) using a Superdex 200 10/300 column (GE Life Sciences) in 25 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... for 2 hours at 45°C and then desalted against 50 mM ammonium acetate using a PD MidiTrap G-10 column (size exclusion limit: 700 Da; GE Healthcare, Sweden) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 or 15 % 29:1 acryl:bis-acrylamide) and transferred on Amersham™ Protran™ 0.45 μm nitrocellulose membranes (GE Healthcare, Cat# 10600033) at 4°C for 1 hr at 110V ...
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bioRxiv - Microbiology 2022Quote: ... The leukocyte-depleted red blood cell pellet was then resuspended in 10 ml PBS and 5 ml were carefully layered on 6 ml of 70% (v/v) isotonic Percoll cushion (GE Healthcare, UK). After centrifugation at 1200 x g for 15 mins ...
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bioRxiv - Systems Biology 2024Quote: ... by SDS-PAGE (10 %, 15 %, 90 µg) and transferred onto nitrocellulose membranes (0.2 µm) (GE Healthcare Life Sciences, Chalfont St Giles, UK) using the semi-dry TransBlot® Turbo™ transfer system (Bio-Rad Laboratories ...
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bioRxiv - Biophysics 2023Quote: ... This clarified sample was filtered (0.2 µm) and loaded onto a cation exchange chromatography SP FF 16/10 column (GE Healthcare Life Sciences) using the AKTA FPLC system ...
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bioRxiv - Pathology 2024Quote: ... Proteins were separated by SDS-PAGE (10 % polyacrylamide gel) and transferred to a PVDF membrane (RNP303F, GE Healthcare, Little Chalfont, Buckinghamshire, UK) in a semi-dry blotter (TE70X ...
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bioRxiv - Immunology 2024Quote: ... was incubated at room temperature for 20 minutes with a 1.2-fold molar excess of ADI-46143 Fab and the complex was purified by SEC on a Superdex 200 Increase 10/300 GL (GE Healthcare Biosciences) in 2 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... Complexes were incubated for 20 minutes at 23 ℃ before further purification via SEC on a Superdex 200 Increase 10/300 GL (GE Healthcare Biosciences) in 2 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... to 20 mg/mL and purified by size exclusion chromatography on a Superdex 75 10/300 GL column (GE Healthcare Life Sciences). Fractions containing pure Ras protein were pooled and concentrated to 20 mg/mL and stored at –78 ºC ...