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Citations for GE Life Sciences :
3501 - 3550 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... was finally purified in PBS 1X by size exclusion chromatography (SEC) on a Superdex 75 10/300 HP column (GE Healthcare; Milan, Italy) and purity assessed by 15% SDS-PAGE using Bio Rad Precision Plus Protein All Blue Standards (10-250 kDa ...
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bioRxiv - Neuroscience 2020Quote: The human neuroblastoma SH-SY5Y cells purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan) were maintained in MEM/F-12 mixture containing 10% fetal bovine serum (HyClone; GE Healthcare Life Sciences) and 1% penicillin-streptomycin ...
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bioRxiv - Biophysics 2020Quote: ... concentrated again (10kDa Amicon®Ultra-4 centrifugal filters) and further purified via gel filtration (Superdex-200 10/300, GE Healthcare Life Sciences). After removal of the poly-E tag with the 3C protease (MERCK) ...
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bioRxiv - Biophysics 2020Quote: ... the AF555- or AS635P-conjugated and biotinylated H57-scFVs were purified by gel filtration using Superdex 75 (10/300 GL, GE Healthcare Life Sciences). Fractions containing monomeric ...
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bioRxiv - Biophysics 2020Quote: ... AF555-conjugated I-Ek/MCC complexes (either C-terminally extended with 2xHis6 or biotin) were subjected to gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) to remove an excess of unreacted MCC-AF555 ...
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bioRxiv - Biophysics 2020Quote: ... concentrated again (10kDa Amicon®Ultra-4 centrifugal filters) and further purified via gel filtration (Superdex-200 10/300, GE Healthcare Life Sciences). After removal of the poly-E tag located on the “alive” subunit with 3C protease (MERCK ...
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bioRxiv - Biochemistry 2020Quote: ... the reaction mixture was applied to a Superdex 200 Increase 10/300 GL gel filtration column (GE Healthcare Life Sciences #28-9909-44) to remove excess glutaryl-CoA/succinyl-CoA ...
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bioRxiv - Microbiology 2021Quote: Analytic SEC was adopted to investigate the oligomeric state of AmnC and its variants using a Superdex 200 10/300 GL column (GE Healthcare, United States). The column was pre-equilibrated with analysis buffer (50 mM Tris-Cl ...
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bioRxiv - Biochemistry 2021Quote: ... Selected fractions containing the N protein were concentrated and loaded on two connected Superdex 200 10/300 GL size exclusion columns (GE Healthcare Life Sciences) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... The self-assembled crude octamers were purified by size-exclusion chromatography on a Superdex-200 10/300 GL column attached to an AKTA FPLC (GE Healthcare, Chicago, IL). Pure fractions were identified by 15% SDS-PAGE after coomassie staining ...
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bioRxiv - Biochemistry 2020Quote: ... a single 0.5 ml aliquot of the final protein was thawed and analyzed by analytical size-exclusion chromatography using a 10/300 Superdex200 analytical column (GE Healthcare, Chicago, IL), with a flow rate of 0.5 ml/min.
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bioRxiv - Biochemistry 2020Quote: ... The products were separated by electrophoresis on a 10% polyacrylamide gel under denaturing conditions and subjected to autoradiography for quantitation on the Typhoon imaging system (GE Healthcare Life Sciences) and were analysed using Quantity One software (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: Approximately 33 mg of CBL or 10 mg of mSLAP2 were loaded individually onto a HiLoad 26/60 Superdex 75 gel filtration column (GE Healthcare Life Sciences) equilibrated with GF buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... connected to a Shimadzu HPLC unit (Shimadzu Corporation, Kyoto, Japan) with a Superdex 200 Increase 10/300 GL SEC column (GE Healthcare Life Sciences) at constant 10°C temperature and equilibrated with the SEC buffer with and without 2 mM CaCl2 ...
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bioRxiv - Biophysics 2021Quote: Size Exclusion Chromatography - Multi Angle Light Scattering Experiments were performed with a pre-equilibrated analytical SEC column (Superdex 200 10/300 GL; GE Healthcare Life Sciences) with protein buffer ...
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Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: ... We pooled fractions containing the purified protein, desalted the pooled fractions using PD-10 desalting columns packed with Sephadex G-25 resin (17085101, Cytiva (formerly GE Healthcare Life Sciences), Marlborough ...
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bioRxiv - Biophysics 2020Quote: ... SHP-1 was further purified by size-exclusion chromatography on an AKTA fast protein liquid chromatography system equipped with a Superdex S200 10/300 GL column (both from GE Healthcare Life Sciences) equilibrated with 10 mM Hepes (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... Peak fractions were pooled and further purified by gel filtration using a Superdex 200 Increase 10/300 GL (GE Healthcare, 28-9909-44) column equilibrated with 20 mM HEPES ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... cells using plasmid pT7-7 and purified using ion-exchange on a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Uppsala, Sweden) (75) ...
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bioRxiv - Plant Biology 2021Quote: ... the supernatant was loaded onto a HisTrap HP 1-mL affinity column and eluted using a gradient of 10 to 500 mM imidazole in buffer B using an ÄKTA start FPLC module (GE Healthcare, Uppsala, Sweden). Fractions were analyzed by SDS–polyacrylamide gel electrophoresis ...
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bioRxiv - Plant Biology 2020Quote: ... Obtained samples were fractionated on the column Superose 6 10/300 GL (50 kDa - 5 MDa range, GE Healthcare Life Sciences, Äkta FPLC) at the rate 0.5 ml/min in 20 mM Tris-HCl pH7.4 and 150 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... This was performed using an purifier system connected to a Superose 6 Increase 10/300 GL column (both from GE healthcare, Solingen, Germany) at a flow rate of 0.5 ml · min−1 in running buffer (50 mM Tris/HCl (pH 8.0) ...
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bioRxiv - Cancer Biology 2021Quote: ... The concentrated supernatant was loaded on a Tricorn 10/300 Sepharose 4 Fast Flow (S4FF) size exclusion chromatography column (GE Healthcare, Buckinghamshire, UK) connected to the ÄKTA prime system (GE Healthcare) ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 10–12 % SDS-polyacrylamide gel electrophoresis gel (SDS-PAGE; Carl Roth) and transferred to nitrocellulose membranes (GE Healthcare Life Science). Membranes were blocked for 1 h with 5 % non-fat milk in PBS-T (PBS with 0.1 % Tween-20 ...
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bioRxiv - Cell Biology 2020Quote: ... The fractions containing the eluted protein were pooled and immediately subjected to buffer exchange using HiPrep™ 26/10 desalting column on an AKTA FPLC purification system (GE Healthcare, USA) into storage buffer (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2020Quote: ... the Mono Q elutions containing desired proteins were further purified by concentration on Amicon Ultra filters followed by size-exclusion chromatograph using a Superdex 200 10/30 GL column (GE Healthcare Life Sciences) in GF150 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... Protein separation was carried out by SDS-PAGE in 10% bis-acrylamide gels and transferred to nitrocellulose membranes (Amersham Protran; pore size, 0.45 μm; GE Healthcare, Chicago, IL, USA). The membranes were blocked with 5% fat free milk in Tris-buffered saline (TBS ...
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C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stressbioRxiv - Cell Biology 2020Quote: Gel filtration was performed using fast protein liquid chromatography (AKTA-FPLC system) on a Superose 6 10/300 GL column (GE Healthcare Life Sciences) as described previously (Hořejší et al. ...
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bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample (at a concentration of 3 mg·ml−1) were loaded onto either a Superdex 75 or 200 HR 10/300 GL columns (GE Healthcare, Madison, WI, USA), equilibrated in 25 mM Hepes (pH 7.8) ...
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bioRxiv - Biochemistry 2022Quote: ... Mpro was eluted with 300 mM imidazole in the lysis buffer and then purified by size-exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL column (GE Healthcare Life Sciences) equilibrated in 50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein sample was then purified by size-exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL (GE Healthcare Life Sciences) equilibrated in 20 mM HEPES pH 7.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein sample was then purified by size- exclusion chromatography using a pre-packed Superose 6 Increase 10/300 GL (GE Healthcare Life Sciences) equilibrated in 50 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... lysates in PBS with protease and phosphatase inhibitor cocktails were injected (500 µL per injection, corresponding to 1–3 mg total protein) onto a Superose® 6 10/300GL column (GE Life Sciences) equilibriated with PBS (pH 7.2 ...
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bioRxiv - Biochemistry 2022Quote: ... the crosslinked-sample was again separated with a flow rate of 0.5 ml/min on a Superdex 200 10/300 GL column (GE Healthcare, Little Chalfont, UK), previously equilibrated with buffer S.
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bioRxiv - Biochemistry 2022Quote: ... pooled and concentrated to 10 ml and applied to a 320 ml size exclusion column (HiLoad ® 26/600 Superdex ® 200, GE Healthcare). The column was equilibrated with 30 mM PIPES pH 7.4 buffer containing 100 mM NaCl + 1 mM TCEP+ 15 μM PLP and eluted with the same buffer ...
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bioRxiv - Immunology 2019Quote: Protein samples were subjected to 10% SDS-PAGE and the proteins were then electrophoretically transferred to Hybond-ECL nitrocellulose membranes (GE Healthcare, Amersham, UK). After blocking with 1% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... Cleavage of the GST tag to induce cleaved fusion proteins was performed using PreScission protease (10 U/mg of fusion protein, GE Healthcare, Uppsala, Sweden) or thrombin protease (10 U/mg of fusion protein ...
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bioRxiv - Immunology 2022Quote: ... The spike proteins were purified from the supernatants on complete™ His-Tag Purification Resin before further purification with Superose 6 increase (S6i) 10/300 column (GE Healthcare Biosciences). The H1 HA gene derived from A/Michigan/045/15 H1 was cloned into pcDNA3.4 vector and contains a GCN4 trimerization domain (folded ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were subsequently washed with 1 x TBS/T for 30 min in 10 min intervals prior to incubation in ECLTM (GE Healthcare, cat# RPN2106) and exposure by film (FUJIFILM Super RX ...
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bioRxiv - Cell Biology 2019Quote: Parasites were grown to late stage schizonts and isolated using a Percoll density gradient whereby culture was added to 60% buffered Percoll solution in supplemented RPMI (10 mM NaH2PO4, 143 mM NaCl, Percoll [GE Healthcare Bio-Sciences]). Purified schizonts at 1-2% parasitemia were added to RBCs with a final haematocrit of 1% ...
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bioRxiv - Biochemistry 2020Quote: Proteins were expressed and purified as described and then passed over an AKTA pure with an inline Superdex 200 Increase 10/300 GL column (GE Healthcare Life Sciences), miniDAWN TREOS ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing TcB-TcC were subjected to SEC using a Superdex 200 10-300 or a Superdex 200 16-60 column (GE Healthcare Life Sciences) equilibrated in gel filtration buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were isolated and used for the separation in the first dimension with Immobiline Dry Stripes (pH range 3-10 NL, 18 cm, GE Healthcare, Uppsala, Sweden) [20] ...
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bioRxiv - Biochemistry 2020Quote: ... TFF-treated culture supernatant was adjusted to 25 mM imidazole and applied to a 10 ml Ni Sepharose High Performance nickel-charged column (GE Healthcare, Chicago, IL) previously equilibrated in Buffer A + 25 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... IMR-32 (ATCC, CCL-127) and BHK-21 cells (ATCC, CCL-10) were maintained in minimal essential medium (MEM) (GE Healthcare Life Sciences) supplemented with 10% FBS (Life Technologies ...
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bioRxiv - Plant Biology 2019Quote: ... The soluble fraction and up to two additional mycelial washes were pooled and lyophilized to concentrate elicitor activity then desalted at least twice using PD-10 desalting columns (GE Healthcare, Chicago, USA) until salt and other small molecules were removed ...
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bioRxiv - Physiology 2021Quote: ... on an ÄKTA Pure Protein Purification System equipped with a Superdex 75 10/300 GL gel-filtration column and a 100 µL loop (GE Healthcare Life Sciences). Buffer was 10 mM HEPES ...
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bioRxiv - Synthetic Biology 2021Quote: ... that had been pre-equilibrated with 10 column volumes of binding buffer (1x PBS, 5mM DTT) using a AKTA FPLC (GE Healthcare, Piscataway, NJ). The column was washed with 10 column volumes of binding buffer and protein was eluted in 100% elution buffer (50 mM Tris ...
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bioRxiv - Synthetic Biology 2020Quote: ... The lysate was then subjected to nickel-immobilized metal affinity chromatography (IMAC) using a HisPrep™ Fast Flow 16/10 column (GE Healthcare, USA) equilibrated with equilibration buffer (20 mM NaH2PO4 ...
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bioRxiv - Microbiology 2020Quote: ... and same protein quantities were separated on a 10% SDS-PAGE gel under reducing conditions and transferred to 0.45 μm PVDF blotting membrane (GE healthcare, Chicago, IL, USA). Total ERK1/2 and phospho-ERK1/2 were detected using p44/42 MAPK antibody (1:1000 in 5% BSA/TBS-T ...