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Citations for GE Life Sciences :
3351 - 3400 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... The membranes were subsequently washed three times for 10 minutes each with TBST after which signal development was performed with ECL Select Western Blotting Detection Reagent (GE Healthcare). Images were acquired using the ChemiDocImager (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... The complex was desalted by a Zeba spin desalting column (7 kDa MWCO) and further purified by size exclusion chromatography using a Superose 6 10/300 column (GE Healthcare). Fluorescein-labeling efficiency was analyzed by SDS-PAGE and fluorescence gel imaging (Amersham Typhoon FLA 9500 ...
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bioRxiv - Microbiology 2020Quote: ... S protein was purified by running the supernatant through a streptactin column and then by size exclusion chromatography using a Superose 6 increase 10/300 column (GE Healthcare Biosciences). Protein fractions corresponding to the trimeric S protein were collected and concentrated.
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bioRxiv - Biophysics 2021Quote: ... Excess free dye was removed by size-exclusion chromatography using the Superdex® 200 Increase 10/300 GL (prep-grade) gel filtration column (GE Healthcare) and gel filtration buffer at a flow rate of ~0.4 mL/min for separation of free dye from labeled protein ...
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bioRxiv - Biophysics 2020Quote: ... The eluate was subjected to size-exclusion chromatography on a 20 ml Superdex 200i 10/300 GL column (GE Healthcare Life Sciences) in a buffer containing 10 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Cell Biology 2020Quote: ... Elutions with CAV1 were concentrated and further purified by size exclusion chromatography using Superpose 6 Increase 10/300 GL (GE Healthcare, Illinois) equilibrated with a buffer composed of 200 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... 150 μL GIT1-CC or β-Pix-CC protein sample at 50 μM was loaded into a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes were subsequently cultured in complete RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS; GE Healthcare Life Sciences, Logan, UT), 0.1 mg/ml penicillin-streptomycin ...
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bioRxiv - Immunology 2022Quote: ... BG505 Env trimer was purified from transfected cell supernatants as described47 by 2G12 immunoaffinity and size-exclusion chromatography (SEC) with a Superose 6 16/600 column followed by Superdex 200 Increase 10/300 GL column (GE Life Sciences).
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bioRxiv - Neuroscience 2020Quote: ... BV2 cells were seeded in 24 or 6 well plates 24 hours prior to the transfection assay in high glucose DMEM supplemented with 10% fetal bovine serum (FBS, GE Healthcare Hyclone) and 1% antibiotic-antimycotic (Gibco ...
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bioRxiv - Immunology 2019Quote: ... the rSsEno oligomeric state was analyzed by analytical size exclusion chromatography on a Superdex 200 GL 10/30 column (GE Healthcare LifeSciences) coupled to a ÄKTA Prime Plus (GE Healthcare LifeSciences ...
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bioRxiv - Cell Biology 2019Quote: ... 150 mM NaCl and 1.2% CHAPS and size fractionated on a Superdex 200 Increase 10/300 GL column (GE Healthcare, Pittsburgh, PA). Fractions were immunoblotted with anti-Bax antibody (Santa Cruz ...
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bioRxiv - Microbiology 2019Quote: ... Samples were separated on 10% SDS PAGE gels together with a high-range rainbow marker (Amersham ECL High-Range Rainbow Molecular Weight Marker, GE Healthcare, UK). Separated proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Bioengineering 2019Quote: ... 1 mM sodium pyruvate and 3.7 g/l NaHCO3) supplemented with 10% HyClone Bovine Growth Serum (GE Healthcare Life Sciences, Cat. # SH30541.03). Human HCT116 cells (ATCC CCL-247TM ...
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bioRxiv - Biochemistry 2019Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Cell Biology 2019Quote: Size exclusion chromatography experiments with HeLa cell extracts were performed with a pre-packed Superose 6 10/300 GL column (GE Healthcare ©). The column was equilibrated with buffer A (20 mM Hepes ...
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bioRxiv - Microbiology 2019Quote: ... The concentrated protein complex was further purified using size-exclusion chromatography with a Superose-6 Increase 10/300-GL (GE Life Sciences) column equilibrated in Buffer B (50 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... The LytH protein was further purified using size-exclusion chromatography with a Superdex 200 Increase 10/300 GL column (GE Life Sciences) equilibrated in Buffer C (25 mM HEPES ...
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bioRxiv - Microbiology 2019Quote: ... Virus labeling was performed with Alexa Fluor™A647 succinimidyl ester in a 1:10 molar ratio and was then purified through Nap-5 desalting column (GE Healthcare). Labeled viruses were aliquoted and stored at −80°C ...
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bioRxiv - Biophysics 2019Quote: ... The unbound protein fraction containing biotin-PS-CFP2 was again purified via gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences). The monomeric biotin-PS-CFP2 was stored in 1 x PBS at −80 °C.
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bioRxiv - Molecular Biology 2019Quote: ... Affinity-purified proteins were subjected to size-exclusion chromatography on a Superdex 200 10/300 column or on a Superose 6 5/150 column (GE Healthcare, Germany) in gel filtration buffer (50 mM Hepes pH 8.0 ...
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bioRxiv - Biochemistry 2019Quote: ... and 5 mg of β-galactosidase G5635-5KU were independently applied to a Superdex 200 10/300 GL analytical gel filtration column (GE Healthcare) equilibrated in 20 mM HEPES KOH ...
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bioRxiv - Neuroscience 2019Quote: ... α-syn was centrifuged at 12000g for 5 minutes and the supernatant loaded onto a Superdex™ 200 10/300 GL column (GE Healthcare). The column was eluted with PBS at a flow rate of 0.5 mL/minute ...
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bioRxiv - Microbiology 2019Quote: ... Prior to infection HFFs were grown to confluency in DME supplemented with 10% v/v cosmic calf serum (GE Healthcare, New Zealand) (D10) ...
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bioRxiv - Immunology 2021Quote: ... iPSC-moDC and MDM were washed with T cell expansion medium (RPMI supplemented with 10% HyClone FBS (GE Healthcare, Chicago, IL, USA), 1000 U/mL Penicillin/streptomycin ...
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bioRxiv - Cell Biology 2021Quote: ... The eluted Cavin4a-FL and Cavin4b-FL proteins were loaded on size exclusion chromatography column Superose™ 6 Increase 10/300 GL column (GE healthcare) pre-equilibrated with 0.22 μm filtered 50 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant containing biotinylated H57-scFV was further purified via gel filtration using Superdex 75 (10/300 GL, GE Healthcare Life Sciences). Finally ...
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bioRxiv - Bioengineering 2021Quote: ... The conjugated antibody was purified by SEC (Superdex 200 increase 10/300 GL, GE Healthcare, solvent: PBS, flow rate = 0.6 mL/min) to afford an antibody–linker conjugate [1.91 mg ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated and analysed by size-exclusion chromatography on a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples at >1 mg/ml (unless otherwise states) were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2020Quote: ... Small- (Superose 6 10/300 GL) or large-scale (Superose 6 pre-packed XK 26/70) SEC columns (GE Healthcare, Chicago, IL) were run with 500 µL or 15 mL of supernatant ...
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bioRxiv - Biochemistry 2020Quote: Samples contain 50 μg total protein were loaded on a 10 % or 16.5 % acrylamide gel for SDS-PAGE and then transferred to a nitrocellulose membrane (GE Life Sciences #10600002). Membranes were incubated in blocking buffer (Thermo Scientific #37539 ...
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bioRxiv - Biochemistry 2020Quote: ... equilibrated in protein buffer (10 mM Tris-HCl pH 8.0, 150 mM NaCl) and connected to an Äkta Purifier FPLC system (GE Healthcare, Boston, U.S.A.). The column was washed with five column volumes and subsequently eluted with three column volumes of protein buffer containing 5 mM d-desthiobiotin ...
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bioRxiv - Biochemistry 2020Quote: SUMOylated Hsf1 purification: After big scale SUMOylation the sample was subjected to size-exclusion on Superdex™200 10/300 GL column (GE Healthcare), equilibrated with Hsf1 SEC buffer (25 mM HEPES/NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: A 100 µL sample was loaded on to a Superdex S200 Increase 10/300 GL gel-filtration column (GE Healthcare Life Sciences) pre-equilibrated with 50 mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated by SDS-gel electrophoresis (10% Acrylamide) and transferred to nitrocellulose membrane (Amersham™ Protran™ 0,45μm NC, GE Healthcare Lifescience). Quality of transfer was confirmed by staining with Ponceau-S solution (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... Protein samples at >1 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein samples at concentrations >5 mg/ml were loaded onto a Superdex™ 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare) in 20 mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... BG505 trimer was purified from supernatant by 2G12 Fab immunoaffinity chromatography followed by SEC using a Superdex 200 Increase 10/300 GL column (GE Life Sciences) running in TBS (20mM Tris pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... The resulting pellet was resuspended in TET and complexes were further separated by Superose 6 10/300 column chromatography in TET buffer with 150 mM NaCl using an AKTA Pure system (GE Life Sciences). The sample collected from the column was used for microscopy and visualized by SDS-PAGE with silver staining (ProteoSilver™ Plus Silver Stain Kit) ...
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bioRxiv - Microbiology 2020Quote: ... 500 μL of sample at 1.5 mg/mL was passed over a SEC column (Superdex 200 Increase 10/300 GL; GE Healthcare Life Sciences) at a flow rate of 0.15 mL/min in buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Biochemistry 2021Quote: ... Cleaved topo VI fractions were pooled and concentrated using Amicon Ultra 15 mL centrifugal filter units (30 kDa cut off, MilliporeSigma) before being passed down a Superose 6 10/300 (GE Life Sciences) column in Buffer C (20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... The protein was dialyzed against PBS and subjected to size-exclusion chromatography using a Superdex 200 Increase 10/300 GL (Sigma, GE Healthcare product) column on an AktaTM Pure (GE ...
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bioRxiv - Microbiology 2021Quote: ... The resulting pellet was resuspended in TET and complexes were further separated by Superose 6 10/300 column chromatography in TET buffer with 150 mM NaCl using an AKTA Pure system (GE Life Sciences). The sample collected from the column was used for microscopy ...
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bioRxiv - Microbiology 2019Quote: ... The alkylation reactions were then quenched with 40mM DTT for 10 minutes and then samples precipitated on to SeraMag Speed Beads (GE Healthcare, USA) with ethanol (final concentration 50% v/v) ...
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bioRxiv - Cancer Biology 2020Quote: ... CRL-1555) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% HyClone cosmic calf serum (CCS; GE Healthcare Life Sciences), 2 mM L-glutamine (Life Technologies) ...
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bioRxiv - Biophysics 2021Quote: ... Proteins were purified to near homogeneity over consecutive running on MonoQ ion exchange column and Superdex 200 increase 10/300 GL pre-packed Tricorn column (GE Healthcare, Sweden) in 6 M urea ...
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bioRxiv - Biophysics 2020Quote: ... For ion exchange chromatography (IEX) the protein was loaded onto a HiPrep Q FF 16/10 anion exchange column (GE Healthcare, Sweden) and eluted against a linear gradient of 7 column volumes (CV ...
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bioRxiv - Cell Biology 2021Quote: ... 10% (v/v) glycerol and 1 mM DTT) and injected onto a MonoQ 5/50 GL column (GE Healthcare and Life Sciences) at 1 mL/min ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were separated by a 10% SDS-PAGE and transferred to a nitrocellulose membrane (Amersham Protran 0.45 μm NC; GE Healthcare Life Science). The membranes were blocked in 1x TBS-0.1% Tween-20 (TBS-T ...