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Citations for GE Life Sciences :
2651 - 2700 of 3691 citations for 8 10 Heneicosadiynoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension volume was adjusted to 33.4 mL with PBS and 10 mL of 100% isotonic Percoll (9 mL Percoll PLUS (GE Healthcare), 1 mL 10x PBS without Ca and Mg ...
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bioRxiv - Cancer Biology 2023Quote: ... Biotinylated proteins were captured from lysates (500 µg per replicate) using 10 μg streptavidin sepharose (GE healthcare, Waukesha, Wisconsin, USA) for 8 h ...
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bioRxiv - Biophysics 2023Quote: ... All proteins were further purified using size-exclusion chromatography (ÄKTA pure system, Superdex 75 Increase 10/300 GL, GE Healthcare). The purified protein was split into aliquots and stored at -80 °C prior to the measurements.
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bioRxiv - Biophysics 2023Quote: ... the sample was clarified 400,000 x g for 30 minutes and applied to a Superdex 200 Increase 10/300 GL (GE Healthcare) gel filtration column equilibrated in G-buffer containing 0.2 M ammonium acetate ...
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bioRxiv - Biochemistry 2023Quote: ... and the concentrate was loaded onto a size-exclusion chromatography (SEC) column (Superose 6 Increase 10/30 GL, GE Healthcare) connected to a Shimadzu high-pressure liquid chromatography instrument ...
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bioRxiv - Immunology 2023Quote: ... lysates and immunoprecipitates were separated by SDS-PAGE with a 10% separation gel and transferred to nitrocellulose membranes (GE Healthcare). Following transfer ...
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bioRxiv - Biophysics 2023Quote: ... The biobeads were removed and the sample was loaded onto a Superose6 Increase 200 10/300 GL column (GE Healthcare) equilibrated to cryo-EM buffer (50 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was finally purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE Healthcare) with buffer E and concentrated to 10 mg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... MEMO1 was additionally purified by size exclusion chromatography on a Superdex 75 10/300 GL Increase column (GE Life Sciences) in a buffer containing 50 mM HEPES-Na ...
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bioRxiv - Biochemistry 2023Quote: ... and loaded onto a SuperoseTM 6 Increase 10/300 GL gel-filtration column (29- 0915-96, GE Healthcare, Uppsala, Sweden) equilibrated with 20 mM HEPES ...
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bioRxiv - Bioengineering 2022Quote: ... Eluted protein was dialyzed to PBS+ (10 mM phosphate pH 7.4 + 300 mM NaCl) and further purified by size-exclusion chromatography on a Superdex 75 10/300 (GE Healthcare) in PBS+ on an Agilent infinity II system ...
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bioRxiv - Biochemistry 2022Quote: ... Column flow-through was concentrated via centrifugal concentrator (10k MWCO) and applied to a Superdex 200 10/300 (GE Healthcare) gel filtration column pre-equilibrated in 50 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare, Chicago, IL) in a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2023Quote: ... The solution was then passed over a Superose 6 Increase 10/30 size-exclusion chromatography column (GE Healthcare Life Sciences) that was preequilibrated in 50 mM phosphate buffer pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: His-purified CsoSCA-MBP-his was run on a Superose 6 Increase 10/300 GL size exclusion column (GE Healthcare) to remove MBP-his contamination ...
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bioRxiv - Biochemistry 2023Quote: ... were injected at flow rate of 0.4 ml min−1 to a Superdex 75 Increase 10/300 GL column (GE Healthcare) equilibrated with buffer containing 20 mM Tris-Cl pH 7.5 ...
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bioRxiv - Biophysics 2023Quote: ... the proteins were further purified via size exclusion chromatography using a GE Superdex200 Increase 10/300 GL column (GE Healthcare) in running buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... Monomeric forms were purified from potential oligomeric species and salt using a Superdex 75 10/300 GL column (GE Healthcare) at a flow rate of 0.5 mL min−1 ...
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bioRxiv - Biochemistry 2023Quote: Protein molecular mass at the native state was determined by gel filtration using a Superdex200 10/300 column (GE Healthcare). A gel filtration calibration kit (high molecular weight ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were separated by size exclusion chromatography on a Superose 6 3.2/300 column (Cytiva) equilibrated in SEC buffer using an ÄKTA Purifier 10 FPLC system (GE Healthcare). After the column dead volume (0.35 column volumes) ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were eluted with 500 mM imidazole followed by buffer exchange to buffer (50mM Tris, 300 mM NaCl, pH 8.0) with HiTrap 26/10 Desalting column (GE Healthcare). To obtain protein without sumo tag ...
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bioRxiv - Biochemistry 2023Quote: ... and GBP2FLK51A were analyzed by size exclusion chromatography using a Superdex 200 increase 10/300 GL column (GE Healthcare/Cytiva). 20 µM protein was incubated at 4 °C overnight with 200 µM of the corresponding nucleotide ...
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bioRxiv - Biochemistry 2023Quote: ... pH 8.0) and further purified via size exclusion chromatography (SEC) using a Superdex Increase 75 10/300 GL column (GE Healthcare) on ÄKTAxpress (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... The cells were grown and maintained in DMEM/F12 medium (ATCC 30-2006) supplemented with 10% heat-inactivated fetal bovine serum (FBS - SH30071.03, HyClone-GE healthcare), 0.5 mg/ml G-418-sulfate (10131-027 ...
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bioRxiv - Microbiology 2023Quote: ... 200 mM NaCl and 2 mM TCEP using a Superdex-200 10/300 GL size-exclusion column from GE Healthcare. All protein concentrations were diluted to 1.7 mg/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... 23S rRNA was purified from total RNA by size-exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) eluted at 0.4 mL/min with 300 mM ammonium acetate ...
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bioRxiv - Biochemistry 2023Quote: ... The untagged protein was further purified by gel filtration chromatography using a Superdex 200 10/300 GL column (GE Healthcare), eluted in buffer E (25 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... His6-MBP fused FoxP3ΔN variants were purified using Ni-NTA affinity column and Superdex 200 Increase 10/300 (GE Healthcare) SEC column in 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2023Quote: ... HepG2 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Hyclone, GE healthcare) and 1% Penicillin-Streptomycin (PS ...
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bioRxiv - Systems Biology 2023Quote: ... α-Synuclein was then purified by Anion exchange chromatography using a HiTrap® Q FF 16/10 (from GE healthcare). The fractions containing α-Synuclein were dialyzed overnight against water and dried after aliquoting 200 ug into low binding Eppendorf tubes ...
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bioRxiv - Systems Biology 2023Quote: ... α-Synuclein was further purified by Anion exchange chromatography using a HiTrap® Q FF 16/10 (from GE healthcare). Exclusively monomeric α- Synuclein was obtain by size exclusion chromatography using a HiLoad® 26/600 Superdex® 75 prep grade (from GE healthcare) ...
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bioRxiv - Cancer Biology 2023Quote: ... were resolved by SDS–polyacrylamide gel electrophoresis using a 10% acrylamide gel and transferred onto 0.2 μm nitrocellulose membranes (GE Healthcare). After a blocking step (5% non-fat milk in TBS O/N at 4°C) ...
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bioRxiv - Biophysics 2023Quote: ... CypA was purified by subtractive anion exchange chromatography using a 10 mL HiTrap Q HP column (GE Healthcare Life Science) equilibrated with 25 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... The protein sample was further purified by FPLC using a Superose® 6 Increase 10/300 GL column (GE Healthcare) equilibrated in sizing buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was finally purified by size-exclusion chromatography on a Superdex 200 Increase 10/300 GL column (GE Healthcare), pre-equilibrated in SEC buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... followed by size-exclusion chromatography to remove over-crosslinked aggregation on a Superose 6 increase 10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM Tris-HCl (pH 7.2) ...
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bioRxiv - Biochemistry 2023Quote: ... and collected by centrifugation at 15,000 g for 10 min at 20 °C followed by size exclusion chromatography using a PD10 column (GE Healthcare). Importantly ...
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bioRxiv - Biophysics 2023Quote: ... CFTR was purified by gel filtration chromatography at 4 °C using a Superose 6 10/300 GL column (GE Healthcare), equilibrated with 0.006% (w/v ...
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bioRxiv - Immunology 2023Quote: ... Cell suspensions were filtered through a 40-μm cell strainer into RPMI with 10% FBS (GE Healthcare Life Sciences SH30071.03) and incubated for 5 min at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... the sample was filtered and applied to an equilibrated ion-exchange chromatography column (Mono S 10/100 GL, GE Healthcare), and weakly bound proteins were washed out with buffer A ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting elutes containing antibodies were applied to a size exclusion chromatography column (Superdex 200 10/300 GL; GE Healthcare) with PBS served as running buffer and a flow rate of 0.5 mL/min (SI Figure 8) ...
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bioRxiv - Bioengineering 2024Quote: ... Final purification by size exclusion chromatography in PBS (pH 7.4) on a Superdex 200 Increase 10/300 GL column (GE Healthcare).
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bioRxiv - Biochemistry 2024Quote: ... nanobody was eluted using 2.5 ml of base buffer + 500 mM imidazole directly into base buffer equilibrated PD-10 columns (GE healthcare). 3.5 ml of base buffer was then applied to PD10 columns to elute the nanobody protein base buffer without imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... protein was eluted using 2.5 ml of base buffer + 500 mM imidazole directly into base buffer equilibrated PD-10 columns (GE healthcare). 3.5ml of base buffer was then applied to PD10 columns to elute the RECQL5 protein in base buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Anion-exchange chromatography (AEX) was performed using a Q Sepharose Fast Flow Column (HiPrep Q FF 16/10; GE Healthcare) pre-washed with three column volumes of AEX Buffer B (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... The buffer was exchanged to PBS (0.05 M phosphate buffer and 0.150 M NaCl) using PD-10 desalting columns or Midi-Trap G-25 (GE Healthcare). The purity of the proteins was evaluated using SDS–PAGE electrophoresis stained with Coomassie blue.
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bioRxiv - Biochemistry 2024Quote: ... The concentrated sample (0.5 mL final volume) was loaded onto a Superdex 200 increase 10/300 size exclusion column (GE Healthcare) at a flow rate of 0.4 mL/min and the absorbance at 280 nm was monitored ...
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bioRxiv - Biochemistry 2024Quote: ... 50-100 μL of the purified protein samples (concentration ranging 2-15mg/ml) were filtered (with 0.1 μm pore size) and loaded onto a Superdex200 10/300GL column (GE Healthcare) preequilibrated with SEC buffer ...
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bioRxiv - Microbiology 2024Quote: ... Derivatized SCCs were further purified by SEC over a Superdex 75 10/300 GL column (GE Healthcare Bio-Sciences AB). ANDS-labeled SCCs were resolved on BoltTM Bis-Tris 4-12% Gel (ThermoFisher ...
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bioRxiv - Physiology 2024Quote: ... RNA was subjected to 8M urea–10% polyacrylamide denaturing gel and autoradiography using Typhoon FLA 9500 Fluorescence Imager (GE Healthcare). Band intensities were quantified using NIH Image J.