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Citations for GE Life Sciences :
1651 - 1700 of 2545 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... Viral supernatant was harvested 48 h after transfection and filtered through a 0.45 μm polyethersulfone membrane filter (GE Healthcare Whatman). Western blotting of the viral supernatants with anti-V5 antibodies (below ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was incubated with secondary ECL anti-rabbit or anti-mouse IgG HRP-linked antibody (GE Healthcare, Pittsburgh, PA) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The transfer of the RNA from the agarose gel to the nylon membrane (Amersham Hyband™-N+, GE Healthcare, #RPN303B) was done as via capillary transfer of RNA from the denaturing agarose gel to the nylon membrane (Sambrook ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was filtered through a MF-Millipore Membrane Filter (0.45 µm pore size) then loaded on a 5 mL His-Trap HP column (GE Healthcare) pre-equilibrated with buffer A and CSE was eluted with a gradient from 20 mM to 250 mM imidazole in buffer A ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and proteins were transferred to Cytiva Amersham™ Protran™ 0.45 µm NC nitrocellulose blotting membranes (GE Healthcare, Munich, Germany). After blocking with 5% bovine serum albumin (Roth ...
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bioRxiv - Genetics 2024Quote: ... [ptch+] were separated on a 1% agarose gel in 1X TAE supplemented with 6.66% formaldehyde and transferred to a positively charged nylon membrane (Hybond N+, GE Healthcare) using capillary transfer ...
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bioRxiv - Microbiology 2024Quote: RNA was separated on a 1.5% formaldehyde-agarose gel and RNA transfer to a positively charged nylon membrane (Amersham Hybond-N+, GE Healthcare) occurred overnight using a capillary blot system ...
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bioRxiv - Genomics 2024Quote: ... 2 µL of gDNA (containing indicated amounts of DNA) was denatured with 0.5 N NaOH for 15 minutes then spotted on a nitrocellulose blotting membrane (GE Healthcare) and cross-linked at 120,000 microjoules for 20 min using a Stratalinker® UV Crosslinker 1800 (Stratagene) ...
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bioRxiv - Plant Biology 2024Quote: Nuclei extracts from leaves of 3-week-old Arabidopsis plants or 40-day-old rice were separated by SDS-PAGE and then transferred to a 0.2 μm nitrocellulose membrane (GE Healthcare). Proteins were detected with anti-H3K27me1 (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysate was fractionated on an 8% SDS-PAGE and transferred to Amersham Protran 0.45 μm nitrocellulose membrane (GE Healthcare). Blots were incubated in blocking buffer (Intercept® PBS blocking buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 μg of total RNA was separated by denaturing agarose gel electrophoresis and transferred to Hybond XL membrane (GE Healthcare). Membranes were probed with radioactively labeled probes recognizing COX1 exon 4 ...
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bioRxiv - Plant Biology 2024Quote: ... Equal amounts of proteins were separated by sodium dodecylsulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) and transferred onto 0,45 μm nitrocellulose membranes (GE Healthcare) which were then probed with the following primary and secondary antibodies with the indicated dilutions ...
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bioRxiv - Neuroscience 2024Quote: ... 2 µg of protein was immobilized on a nitrocellulose membrane (Amersham) by filtration using a slot blot apparatus (GE Healthcare). The membrane was blocked with 5% skimmed milk and probed with MJFR-14-6-4-2 (Abcam ...
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bioRxiv - Molecular Biology 2024Quote: ... The fraction bound was measured by capturing protein-RNA complexes on Protran nitrocellulose membrane (Whatman, GE Healthcare Bioscience, Pittsburgh, PA) and unbound RNA on a Nylon XL membrane (GE Healthcare Bioscience ...
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bioRxiv - Microbiology 2024Quote: ... and the protein from the other gel was transferred to a 0.45-mm-pore-size nitrocellulose membrane (Hybond-ECL; GE Healthcare) and incubated with mouse α-HIS antibody (Genscript A00186) ...
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bioRxiv - Microbiology 2024Quote: ... 5 µl of total protein were separated on 10% SDS-PA gels which were either blotted onto a nitrocellulose membrane (AmershamTM Protran TM Premium 0.45 µm NC, GE Healthcare) using a Trans-Blot turbo transfer system (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... 20 mM imidazole pH 7.5 was added prior to loading solubilized membranes on a pre-equilibrated 1 mL Ni-NTA column (GE Healthcare) at 1 mL min-1 ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of total lysates were resolved in Tris-Glycine SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare) in 25 mM Tris HCl pH 8.3 ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was filtered through an 0.8 µm membrane and loaded onto a 5 mL HisTrap HP column (GE Healthcare), pre-equilibrated with lysis buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein extracts (30 µg) were separated by SDS-PAGE and transferred to Hybond-C Extra nitrocellulose membranes (GE Healthcare, USA). Details regarding primary and secondary antibodies are listed in Table S5 ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were then washed 3 times for 10 minutes with 1XPBST and imaged using an Amersham Imager 600 (GE Healthcare). To immunoblot for phospho-specific Traip S295 and T325 antibodies in Fig ...
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bioRxiv - Biochemistry 2024Quote: ... The resultant supernatant was filtered through a 0.8 μm membrane before being loaded onto 5 mL HisTrap FF nickel column (GE Healthcare) pre-equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were separated according to size using standard SDS-PAGE and transferred onto a nitrocellulose membrane (GE Healthcare, Amersham, Germany). Membranes were blocked in 5 % skimmed milk/TBS-T and probed with primary antibodies (listed in supplementary table 2 ...
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bioRxiv - Cancer Biology 2024Quote: ... The membrane was washed again three times with TBST 0.1% and incubated with Amersham ECL prime detection reagent (GE Healthcare) or Clarity Max ECL substrate (Biorad) ...
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bioRxiv - Cell Biology 2024Quote: ... The transfer was performed in Towbin buffer with a nitrocellulose membrane (Amersham Protran 0.45, GE Healthcare Europe GmbH, Freiburg, Germany). The blot was blocked with 5% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... Protein were separated by SDS-PAGE and transferred to nitrocellulose membranes (Cat. No. 10600001; GE Healthcare; Chicago, IL, 16 USA). The membranes were incubated with primary antibodies ...
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bioRxiv - Microbiology 2021Quote: Cell wall binding assays of recombinant PBP1 proteins fluorescently labelled with Cy2 bis- reactive dye (GE Healthcare) were performed as previously described (Bottomley et al. ...
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bioRxiv - Microbiology 2020Quote: SPR spectroscopy and calibration free concentration (CFCA) assays were performed using a Biacore T200 device (GE Healthcare) and streptavidin-precoated Xantec SAD500-L carboxymethyl dextran sensor chips (XanTec Bioanalytics GmbH ...
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bioRxiv - Immunology 2021Quote: Purified antibodies targeting S1 were quantified with SPR assay using the BIAcore 8000 system (GE Healthcare, USA) carried out at 25°C in single-cycle mode ...
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bioRxiv - Plant Biology 2020Quote: ... Fifty micrograms of protein were separated on an SDS-PAGE and transferred to an Amersham Hybond-P PVDF membrane (GE healthcare). The rabbit polyclonal antibody to PHO1 [51] and goat anti-rabbit IgG-HRP (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: ... The gDNA samples (5 µg for S9.6 blot and 0.5 µg for ssDNA blot) were blotted onto a positively charged nylon transfer membrane (GE Healthcare Cat# RPN203B) using a slot blot apparatus (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-PAGE was conducted as above with 67 ng of substrate protein per well before the western was conducted using a nitrocellulose membrane (GE healthcare) and 1X NuPage transfer buffer (Thermo) ...
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bioRxiv - Cell Biology 2020Quote: Proteins (30 to 50μg of total cell lysates) were separated in SDS-PAGE gels and transferred to PVDF membranes (Amersham Hybond-P, GE Healthcare). Membranes were then blocked in PBS-T (PBS – 0.1% Tween 20 ...
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bioRxiv - Microbiology 2020Quote: ... Fuji BAS-IIIS imaging plates were exposed to the membranes and read out by an Amersham Typhoon laser scanner (GE Healthcare). As loading control ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 and 200 ng of DNA from each fraction and from unfractionated genomic DNA were blotted on a membrane (Amersham Hybond -N+, GE Healthcare) using a BioDot apparatus (Bio-rad) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were then rinsed three times and treated with the Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare Life Sciences), and blots were analyzed with the ChemiDoc XRS+ System (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... heated to 95°C for 5min and separated on a 10% SDS-PAGE gel before transferring onto a nitrocellulose membrane (Amersham Hybond ECL, GE Healthcare) using BioRad transfer apparatus ...
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bioRxiv - Molecular Biology 2021Quote: ... Low-molecular weight RNAs were separated on 8% or 15% acrylamide/7 M urea gels and transferred to a Hybond N+ membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 or 10 µg of the purified RNA was resolved on formaldehyde agarose gels and transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Developmental Biology 2021Quote: ... which was added to the membrane for 1 min before the signal was detected using the ImageQuantTM LAS 4000 (GE Healthcare). Antibodies and dilutions used ...
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bioRxiv - Biochemistry 2022Quote: ... Immunoblotting was carried out by separating the immunoprecipitates on 10% polyacrylamide gels as described using a Bio-Rad Mini-Gel apparatus and transferring them electrophoretically to Hybond-C Extra membranes (GE Healthcare) using a Trans-Blot apparatus (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times with TBS-T for 10 min each before incubation with Amersham ECL detection reagent (GE Healthcare) and chemiluminescent detection using the Li-COR Odyssey FC imaging system (LI-COR Biosciences) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10-30 μg of protein was electrophoresed on 4-12% (or 12% for OGG1ko blot) Bis-Tris gels (Thermo) before transferring to PDVF membranes (GE Healthcare). Membranes were blocked in 5% milk and blotted with primary and secondary HRP antibodies ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
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bioRxiv - Cell Biology 2020Quote: ... before heating at 95°C for 5 min and samples were separated by SDS-PAGE using a 4-16% acrylamide gradient gel and blotted onto nitrocellulose membranes (Hybond-C Extra, Amersham Bioscience or Optitran BA-S 85, GE Healthcare). The membranes were incubated in the given primary antibody in a dilution of 1:1000 and appropriate HRP-conjugated secondary antibody (Dako ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amounts of protein were resolved by SDS-PAGE using a 10 % acrylamide gel and subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Amersham Hybond, GE Healthcare) via the PerfectBlue™ tank electro blotter (Peqlab ...
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bioRxiv - Genetics 2020Quote: ... Whole cell lysates were quantified and 50 μg were resolved in 4-20% SDS-PAGE and transferred onto nitrocellulose membranes (GE healthcare). The membranes were then incubated with the primary antibodies ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The RNA lanes were blotted through classic upward blot onto a Amersham Hybond N+ membrane (GE Healthcare Life Science, Little Chalfont) by neutral transfer (20× SSC ...
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bioRxiv - Immunology 2021Quote: ... The membrane was removed from the substrates and then exposed to the Amersham imager 600 (GE Healthcare Life Sciences, Marlborough, MA).
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bioRxiv - Plant Biology 2020Quote: ... 1970) stained with Coomassie Brilliant Blue R-250 or electroblotted onto nitrocellulose membranes (0.2-mm pore size; GE Healthcare, Chicago, Illinois). Immunoblotting analyses were performed using specific primary antibodies and a rabbit-specific horseradish peroxidase-conjugated secondary antibody (Agrisera ...