Labshake search
Citations for GE Life Sciences :
1501 - 1550 of 2545 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... Membranes were exposed to X-ray for 5-10 min using a biomolecular imager (ImageQuant LAS4000, GE Healthcare, USA). Relative cytokine content was determined after quantification of the dot intensity using ImageJ.
-
bioRxiv - Immunology 2020Quote: ... 12% or gradient (10-15%) mini precast gels (7bioscience) and to subsequent immunoblotting on PVDF membrane (GE Healthcare Amersham) and blocked with 5% BSA in PBS and 0.1% Tween20 ...
-
bioRxiv - Microbiology 2021Quote: ... and the membrane extract was loaded on a Chelating Sepharose Fast Flow bed resin (∼10 ml; GE Healthcare, UK) previously activated with 200 mM NiCl2 (Sigma ...
-
bioRxiv - Cell Biology 2021Quote: ... Membrane development was performed by an enhanced chemiluminescencebased detection method (ECL™ Prime Western Blotting Detection Reagent, GE Healthcare) and observed using a ChemiDoc MP Imaging System (Bio-Rad) ...
-
bioRxiv - Cell Biology 2021Quote: ... PVDF membranes were washed and revealed using horseradish peroxidase (HRP)-conjugated secondary antibodies and ECL detection reagent (GE Healthcare). The blots were imaged on a Geliance gel imaging systems (PerkinElmer ...
-
bioRxiv - Microbiology 2022Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated anti-rat or anti-mouse (1:2000; GE healthcare) for 1 hour at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... were run on 8% polyacrylamide gel and electrophoretically transferred onto nitrocellulose membrane (RPN303E, Hybond-C-extra, GE Healthcare Europe). The membranes were incubated at 4 °C under gentle shaking for 1 h in blocking buffer (1× TBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated with SDS-PAGE and subsequently transferred to Amersham Hybond-P PVDF Transfer Membrane (RPN303F, GE Healthcare). Membranes were blocked in 5% milk in Tris-buffered saline with 0.01% Tween 20 and incubated with the antibodies listed in Supplemental Table 4 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The membranes were revealed after adding the chemiluminescent substrate using an Amersham Imager 600 device (GE Healthcare, Uppsala, Sweden).
-
bioRxiv - Genomics 2023Quote: ... Yeast cells were harvested by filtration with 0.45 µM Whatman cellulose nitrate membrane filters (GE Life Sciences #7184-004). Ribosome profiling was conducted as detailed in (McGlincy and Ingolia ...
-
bioRxiv - Microbiology 2023Quote: ... or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo) to membranes and imaging on an Amersham Imager 600 (GE Healthcare). Membranes were stripped with Restore PLUS Stripping Buffer (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed 5 more times and the signal was detected by chemiluminescence using ECL Prime (GE Healthcare) on an Amersham Imager 600 detection system (GE Healthcare).
-
bioRxiv - Molecular Biology 2022Quote: ... The membrane was incubated with secondary antibodies and visualized with the ECL Detection System (GE Healthcare, Little Chalfont, UK).
-
bioRxiv - Molecular Biology 2024Quote: Protein lysates were separated on 10% SDS-PAGE gels and then transferred to PVDF membranes (GE Healthcare Life Science). The membranes were blocked in phosphate-buffered saline with Tween 20 (PBST ...
-
bioRxiv - Molecular Biology 2024Quote: ... membranes were developed by SuperSignal™ West Pico PLUS chemiluminescent substrate (Thermo) using ImageQuant™ LAS 4000 (GE Healthcare). Membranes were stripped with 1X ReBlot Plus Strong Antibody Stripping Solution (#2504 ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein extracts were separated in SDS-PAGE gels and transferred to a PVDF membrane (GE Healthcare, Chicago, IL, USA). Western blotting was performed according to standard protocols ...
-
bioRxiv - Molecular Biology 2023Quote: ... The gel was soaked in 1×TBE for 20 min and transferred to a Hybond-N+ membrane (GE Healthcare) for 1 h (15 V ...
-
bioRxiv - Microbiology 2023Quote: ... the membranes were incubated for 1 h with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (GE Healthcare), diluted 1/10,000 in PBST ...
-
bioRxiv - Biochemistry 2023Quote: ... and after extensive washing in PBST the dried membrane was scanned with an imaging system (GE Healthcare Amersham Typhoon). For lectins ...
-
bioRxiv - Cancer Biology 2023Quote: ... Membranes were then washed with 3X with TBST buffer and incubated with appropriate HRP-conjugated secondary antibodies (GE Healthcare) for 1 hour at RT ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were separated by SDS-PAGE using 4% or 5% polyacrylamide gel and transferred to nitrocellulose membranes (GE Healthcare). Membranes were probed with mouse monoclonal antibody generated against HA-tag peptide (YPYDVPDYA ...
-
bioRxiv - Biophysics 2023Quote: ... 11 times through a NucleporeTM Track-Etch Membrane polycarbonate filter of pore size 400 nm (GE Healthcare Life Siences) and diluted to 4 mg/ml in reconstitution buffer ...
-
bioRxiv - Microbiology 2022Quote: ... the membranes were incubated for 1 h with an anti-rabbit secondary antibody conjugated to horseradish peroxidase (GE Healthcare), diluted 1/10,000 in PBST ...
-
bioRxiv - Cancer Biology 2022Quote: ... 15μg of protein were resolved on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under denaturing conditions and transferred to Protran Premium NC 0.45μm membranes (Amersham Biosciences, GE Healthcare). Membranes were blocked for 1h at room temperature (RT ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated on a 7.5% Bis-Tris gel and blotted onto a 0.45 μm nitrocellulose membrane (GE Healthcare Life Sciences ...
-
bioRxiv - Plant Biology 2023Quote: ... which was subjected to SDS PAGE and blotted on a nitrocellulose membrane (Amersham Protran Premium 0.45 μm, GE Healthcare). The blotted membrane was blocked in 5% skimmed milk for 1 hour ...
-
bioRxiv - Bioengineering 2022Quote: ... Membranes were exposed to X-ray for 5-10 min using a biomolecular imager (ImageQuant LAS4000, GE Healthcare, USA). Relative cytokine content was determined after quantification of the dot intensity using ImageJ.
-
bioRxiv - Cancer Biology 2023Quote: ... Proteins were blotted for 80 min (350 mA, room temperature) onto Amersham Protran 0.2 μm nitrocellulose membranes (GE Healthcare). Membranes were blocked in 5% milk in TBS-Tween for at least 2 h and incubated with primary antibodies overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... The membranes were rinsed in PBST and bound antibodies detected with ECL (GE Healthcare Life Sciences, Chicago, IL, USA).
-
bioRxiv - Synthetic Biology 2023Quote: ... Digested DNA was electrophoresed in 1% agarose and transferred by capillarity onto a nylon membrane (Hybond N+, GE healthcare). Membranes were hybridized in Church buffer at 68°C with Cy5-probe ([23]) ...
-
bioRxiv - Biochemistry 2024Quote: ... using 10/12% acrylamide/bisacrylamide (29:1) 0.1% SDS gels and transferred to a 0.2 μm PVDF Hybond-P membranes (Amersham/GE healthcare) at 20V for 1h in a semi-dry Trans-Blot SD cell system (Bio-Rad ...
-
bioRxiv - Molecular Biology 2024Quote: ... resolved on 4–12% NuPAGE gels and transferred to a nitrocellulose membrane (Amersham Protran, GE Healthcare Life Sciences, #10600002) using a wet-transfer system ...
-
bioRxiv - Molecular Biology 2024Quote: ... Samples were subjected to SDS-polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane (GE Healthcare Life Sciences #10600002). Membranes were blocked at room temperature for 1 h with 5% milk in T-TBS (1% Tween 20 - Tris Buffered Saline buffer ...
-
bioRxiv - Microbiology 2024Quote: ... Supernatant containing the solubilised membrane proteins was pooled and applied to a His-TRAP nickel affinity column (GE Healthcare) using an ÄKTA Protein Purification System (Cytiva) ...
-
bioRxiv - Microbiology 2024Quote: ... was used to visualize proteins on an Amersham Imager 600 RGB gel and membrane imager (GE Life Sciences/Cytiva).
-
bioRxiv - Microbiology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to 0.45 µm pore-size nitrocellulose membranes (GE Healthcare Life Sciences). Membranes were blocked at room temperature for 1 h with Tris-buffered saline (TBS ...
-
bioRxiv - Molecular Biology 2024Quote: ... RNA binding was measured by capturing protein-RNA complexes on Protran nitrocellulose membrane (Whatman, GE Healthcare Bioscience, Pittsburgh, PA) and unbound RNA on a Hybond-XL (Cytiva ...
-
bioRxiv - Genetics 2024Quote: ... Membranes were washed with TBS-T for 30 min and the signal was developed by ECL Prime (GE Healthcare). Primary and secondary antibodies are listed in Supplemental Table S3.
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant was filtered through 0.8 µm membranes and applied to a HisTrap HP 5 mL column (GE Healthcare) pre-equilibrated with Buffer A ...
-
bioRxiv - Cell Biology 2024Quote: ... Proteins were resolved by 10 or 12% SDS-PAGE and transferred to a 0.2-µm nitrocellulose membrane (GE Healthcare). The membrane was blocked with 5% non-fat dry milk in TBS-T (20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Cell Biology 2022Quote: Binding experiments and kinetics assays were carried out using a BIACORE 3000 instrument (GE Healthcare, USA). Two different sensor chips were used ...
-
bioRxiv - Neuroscience 2022Quote: ... total RNA was quantified by spectrophotometric assay using a NanoVue Plus spectrophotometer (GE Healthcare, Buckinghamshire, UK). RNA was reverse transcribed using High-Capacity cDNA Reverse Transcription Kit (4368814 ...
-
bioRxiv - Biochemistry 2023Quote: SEC binding assays were performed using a Superdex 200 increase Hiload 10/300 column (GE healthcare) at a flow rate 0.4 ml/min with absorbances monitored at 280 nm ...
-
bioRxiv - Cell Biology 2024Quote: ... ECL kit (GE Healthcare) and SuperSignal West Dura (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: ... The resultant eluate was filtered through a 0.45 μm filtration membrane and then loaded onto a 5 ml SOURCE 15S column (GE Healthcare) equilibrated with buffer I ...
-
bioRxiv - Cancer Biology 2021Quote: ... Digestions were then electrophoresed on a 0.7% agarose gel prior to transfer onto an Amersham Hybond XL nylon membrane (GE Healthcare). Blots were then probed with P32-labeled DNA probes comprised of an internal sequence homologous to the genomic insertion containing the StrepTag exon.
-
bioRxiv - Molecular Biology 2020Quote: 10-25ug of total RNA was resolved on a 12.5% denaturing polyacrylamide gel and transferred to a positively charged nylon membrane (GE Healthcare) using 1x TBE ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were detected by staining with Coomassie Brilliant Blue R250 or by transferring to a PVDF membrane (GE Life Sciences), and staining with Ponceau S dye or detection by immunoblotting ...
-
bioRxiv - Cell Biology 2020Quote: ... the protein fraction in the supernatant was resolved on a 10 % SDS-PAGE gel and transferred to a 0.2-μm nitrocellulose membrane (Amersham, GE Healthcare). The membrane was blocked with 5 % (w/v ...
-
bioRxiv - Genomics 2020Quote: ... The membrane was incubated with 1:10,000 dilution of anti-rabbit IgG conjugated to horse radish peroxidase (HRP; GE Healthcare) diluted in 2.5% skimmed milk in TBS-T or anti-mouse IgG conjugated to HRP (GE Healthcare ...