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Citations for GE Life Sciences :
1751 - 1800 of 2545 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... incubated 5 min at 95 °C before analysis by SDS-PAGE and transfer onto a nitrocellulose membrane (Protran, 45 µm pores, GE Healthcare). DACone adducts onto BLG were visualized by scanning the membrane with an infrared imager (Odyssey ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated on a 10% polyacrylamide gel and transferred to a nitrocellulose membrane (GE life sciences, 1060002) using a Pierce™ Power Station (ThermoFisher) ...
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bioRxiv - Plant Biology 2020Quote: ... DNA samples digested with restriction enzymes were separated in 1% agarose gels and blotted onto Hybond-XL nylon membranes (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... were used for the protein detection and membrane images were taken using Amersham Imager 600 (GE Healthcare Life Sciences, Chicago, IL).
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bioRxiv - Evolutionary Biology 2021Quote: ... Each aliquot was denatured in 0.4 M NaOH and blotted onto a positively charged Nylon membrane (Hybond XC, GE Healthcare, USA) using a vacuum slot blotter (Schleicher-Schuell ...
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bioRxiv - Cell Biology 2020Quote: ... 100 – 300 µg protein was loaded on 10 – 12% SDS-PAGE and transferred at 4°C overnight to a nitrocellulose blotting membrane (A29434119, GE Healthcare) in transfer buffer (25 mM Tris pH 8 ...
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bioRxiv - Microbiology 2020Quote: ... of RNA was resolved on a 1% agarose denaturing gel followed by blotting on a nylon membrane (Amersham Hybond-N+, GE Healthcare). A probe for mCherry or for 4× Broccoli was synthesized by PCR using the 4× Broccoli plasmid as template and the primers prW150-prW151 for Broccoli and prW459-prW460 for mCherry ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins in cell lysates were separated on SDS-PAGE and then transferred electrophoretically onto PVDF membranes (GE Healthcare, Milwaukee, WI, USA) using a transfer cell (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Equal amounts of lysates containing Laemmli buffer were heated at 95°C and electrophoresed on 12% polyacrylamide gels under standard SDS-PAGE conditions before being transferred onto a polyvinylidene fluoride membranes (GE Healthcare). Protein loaded membranes were blocked with 5% (w/v ...
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bioRxiv - Immunology 2020Quote: ... Total protein 20 μg (or 40 μg for SDC3 analysis) was separated by SDS-PAGE and transferred onto nitrocellulose membranes (Hybond, GE Healthcare). Primary antibodies used were ...
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bioRxiv - Cancer Biology 2021Quote: ... and 20 μg were resolved on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under denaturing conditions and transferred to Hybond ECL membrane (Amersham Biosciences, GE Healthcare). Membrane was stained with Ponceau S solution (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... 6 μg of total RNAs were resolved on a 1 % (w/v) agarose gel then transferred onto Amersham Hybond-N+ Membrane (GE Healthcare) by capillary transfer ...
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bioRxiv - Molecular Biology 2022Quote: ... Low-molecular-weight RNAs were separated on 6% acrylamide/7 M urea gels and transferred to a Hybond-XL membrane (GE Healthcare) by electrotransfer ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl of the homogenate was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA) and left to dry out at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... was separated on a denaturing formaldehyde/formamide 1.2 % agarose gel and transferred and UV-crosslinked onto Amersham Hybond™-N membrane (GE Healthcare). RNA was visualized using [32P]-radiolabeled probes targeting mitochondrial RNAs as indicated (see Supplementary Table S1).
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bioRxiv - Cell Biology 2022Quote: ... sjRop1 LPPs and the purified REEP1 proteins from the membrane fractions were further purified by size exclusion chromatography (SEC) on Superose 6 or Superdex 200 (GE Healthcare) columns ...
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bioRxiv - Immunology 2022Quote: ... Samples were resolved by SDS-12% polyacrylamide gel electrophoresis under standard conditions and transferred to a polyvinylidene difluoride (PVDF) membrane (Amersham Hybond, GE Healthcare) using the Trans-Blot turbo transfer system (Biorad) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Each strain was subsequently transferred to a 100 mm Petri dish with an overlay of sterile autoclaved Hybond nylon membranes (84 mm) obtained from Amersham (GE Healthcare). The strains were then allowed to grow for 2-3 weeks on PDA media overlaid with this membrane ...
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bioRxiv - Plant Biology 2022Quote: ... the gels were run in 1× RNA running buffer (1 × MOPS and 6% [v/v] formaldehyde) and blotted onto the Hybond-N+ membrane (GE Healthcare) soaked in 20× SSC (3.0 M NaCl and 0.3 sodium citrate) ...
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bioRxiv - Genomics 2022Quote: ... Samples were then loaded onto a 1% agarose gel containing 3% formaldehyde and 1x MOPS and run at 75 V in 1x MOPS for ~3 hs after which RNA was transferred onto a Hybond N+ membrane (GE Healthcare) overnight ...
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bioRxiv - Microbiology 2022Quote: ... Proteins (40 µg) were run on 10% SDS–PAGE gel and transferred to an Amersham Hybond-ECL nitrocellulose membrane (GE healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 or 10 μg of the purified RNA was resolved on formaldehyde agarose gels and transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed twice in PBS-T before being visualized on a Typhoon Trio Scanner (GE Healthcare, Piscataway, NJ, USA) and quantified with ImageJ (Abramoff et al. ...
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bioRxiv - Plant Biology 2022Quote: ... equal amounts of proteins were separated on a 10% or 12% SDS-polyacrylamide (PA) gel and transferred to nitrocellulose membranes (GE Healthcare). The signal was probed with specific antibodies using Clarity Western ECL Blotting Substrate (Bio-Rad).
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bioRxiv - Physiology 2022Quote: ... Equal amounts (30 μg) of total protein were separated by 10 % SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). After blocking with Tris-Buffered Saline (Sigma-Aldrich ...
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Dysregulated expanded endocannabinoid system as therapeutic targets of amyotrophic lateral sclerosisbioRxiv - Neuroscience 2024Quote: ... and equal amounts of protein were separated on 5–20% SDS‒PAGE gels (Wako) and transferred to Hybond-P membranes (GE Healthcare). The following primary antibodies and dilutions were utilized ...
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bioRxiv - Molecular Biology 2024Quote: ... Labeled proteins were visualized on the PVDF membrane using a Storage Phosphor screen (Molecular Dynamics) and imaged with a Typhoon 9410 Variable Mode Imager (GE Healthcare). Due to background binding and loss of protein in the washes ...
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bioRxiv - Cancer Biology 2024Quote: ... 15-25μg of proteins were run on NuPage 4-12% gradient Bis-Tris gels in NuPAGE™ MES SDS Running Buffer (20X) and wet-transferred to 0.2μM nitrocellulose membranes (GE Healthcare, #10600001). Blots were blocked in Intercept (TBS ...
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bioRxiv - Genomics 2024Quote: ... Aliquots of the extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Amersham/GE Healthcare). The membranes were incubated with primary antibodies in a blocking solution (2% BSA in PBS containing 0.1% Tween 20 (PBS-T) ...
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bioRxiv - Biochemistry 2023Quote: ... After staining with Ethidium bromide to visualize total DNA and gel transfer on a Nylon N+ membrane (GE Healthcare Ref RPN203B) (Qbiogen ...
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bioRxiv - Cell Biology 2023Quote: ... Dried membranes were exposed to BAS storage phosphor screens (Cytiva, 28956475) and scanned using a phosphor imager (Typhoon FLA 9500 GE Healthcare). Signals were analyzed with the ImageQuant TL software (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was then incubated with secondary ECL anti-rabbit or anti-mouse IgG HRP-linked antibody (GE Healthcare, Pittsburgh, PA) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Gels were blot-transferred to a nylon membrane and probed at 60°C with the YCp1.3 DNA labelled with AlkPhos Direct (GE Healthcare®). Chemiluminescent signals of increasing exposure periods were recorded on X-ray films ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were treated with EZ-ECL (Biological industries, Beit-Ha’emek, Israel) and visualized using ImageQuant LAS 4000 (GE Healthcare, IL, USA). Blot intensities were quantified using the FIJI ImageJ software86 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 20-30 μg of protein was electrophoresed on 4-12% (or 12% for OGG1 blot) Bis-Tris gels (Thermo) before transferring to PDVF or nitrocellulose membranes (GE Healthcare). For PAR 10H western blots ...
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bioRxiv - Plant Biology 2023Quote: ... total protein extracts (10 to 20 μg) were subjected to 12% or 15% SDS-PAGE and then transferred to 0.45 μm nitrocellulose membranes (GE Healthcare, 10600003). The anti-CrATG3 polyclonal antibody was produced by injecting the recombinant wild-type CrATG3 protein into a rabbit using standard immunization protocols at the Animal Resource facility from the University of Sevilla ...
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bioRxiv - Molecular Biology 2023Quote: ... the nitrocellulose membranes were exposed to a storage phosphor screen and the screen was imaged using a Typhoon FLA 7000 scanner (GE Healthcare). Total lysate and IP Western blot and 35S signal were quantified using Image J and 35S signal/IP Western blot signal were plotted against pulse time ...
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bioRxiv - Molecular Biology 2022Quote: ... WCE was then run on 5–20% gradient SDS–polyacrylamide gel electrophoresis (PAGE; ATTO) and then transferred to Hybond ECL membranes (GE Healthcare) followed by incubation with indicated antibodies ...
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bioRxiv - Molecular Biology 2023Quote: After Western blot development the nitrocellulose membranes were exposed to a storage phosphor screen and the screen was imaged using a Typhoon FLA 7000 scanner (GE Healthcare). Total lysate ...
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bioRxiv - Cell Biology 2023Quote: Proteins were separated using SDS-PAGE in 10 % Bis-Tris acrylamide/ bisacrylamide gels and transferred to a nitrocellulose membrane (GE Healthcare). The membranes were blocked for 30 minutes with PBS 5 % milk and incubated with the first antibody for 1 hour at room temperature or at 4°C over-night with gentle shaking ...
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bioRxiv - Biophysics 2023Quote: ... Proteins separated based on molecular weight onto the gel were transferred onto a 0.2 μm western blot membrane (GE life sciences) using a thermo scientific transfer cassette for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 35 μg protein from each sample was resolved by SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). The following antibodies were used at the indicated dilutions ...
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bioRxiv - Bioengineering 2023Quote: ... Equal Volume of total soluble proteins were separated on a 15% (w/v) SDS polyacrylamide gel and transferred onto a Hybond-P membrane (RPN303F, GE Healthcare). Nonspecific binding was blocked with 3% (w/v ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was performed using BCA and proteins was separated on SDS-PAGE followed by transfer to PVDF membrane (GE Healthcare). Next ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated for 2-h at room temperature with rabbit HRP-linked IgG (GE Healthcare, Chicago, IL, USA), and a chemiluminescence detection reagent (SuperSignal™ West Pico PLUS Chemiluminescent Substrate ...
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bioRxiv - Cancer Biology 2023Quote: ... and separated by 4-12% Bis-Tris SDS-PAGE electrophoresis (at 180 V, 300 W, 55 min) and transferred onto a nitrocellulose membrane (GE Healthcare). Membranes were blocked in 5% BSA(Sigma ...
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bioRxiv - Genomics 2023Quote: ... The digested DNA fragments were separated by electrophoresis using 1% agarose gels and transferred overnight to Hybond-N+ membranes (GE Healthcare) in 20 × SSC ...
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bioRxiv - Immunology 2022Quote: ... Samples were resolved on 12–15% SDS–PAGE gels and then proteins were wet transferred to Protran nitrocellulose membranes (GE Healthcare) at 400 mA at 4 °C for 1.5 h in 1x Transfer buffer (25 mM Tris base ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were resolved by electrophoresis on 10 or 12% SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membranes (GE Healthcare). Total protein labeling was performed using No Stain Labeling Reagent (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... TCA extracts of total yeast or 2 μg of mitochondrial proteins were loaded on a 12 % SDS-PAGE and transferred onto a nitrocellulose membrane (GE Healthcare). UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD) ...