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Citations for GE Life Sciences :
1901 - 1950 of 2545 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 2 µl of the elutions were subjected to SDS-PAGE and transferred to an Amersham Protran 0.45 μm nitrocellulose blotting membrane (GE Healthcare, Uppsala, Sweden) using a Trans-Blot semi-dry transfer cell (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was wrapped in plastic film and exposed to X-ray film (Amersham Hyperfilm™ ECL, GE Healthcare Limited, Cat.No. 28906836) for 7 min.
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed 3×10mins with TBS-T and 1×10mins with TBS before developing the signal by incubating the membrane in Amersham Hyperfilm ECL for 5 minutes (GE Healthcare, UK).
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bioRxiv - Plant Biology 2023Quote: ... Membrane imaging was carried out with an ImageQuant LAS 4000 or an ImageQuant 800 luminescent imager (GE Healthcare Life Sciences, Piscataway, NJ). Rubisco loading control was stained using Ponceau S (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... Membrane development was performed by an enhanced chemiluminescence-based detection method (ECL™ Prime Western Blotting Detection Reagent, GE Healthcare, Fisher Scientific) using a ChemiDoc MP system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Membrane development was performed by an enhanced chemiluminescence-based detection method (ECL™ Prime Western Blotting Detection Reagent, GE Healthcare, Fisher Scientific) using a ChemiDoc MP system (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... All samples were loaded with the same amount of total protein (40μg) and separated on 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto PVDF membranes (GE Healthcare, Buckinghamshire, UK) using a Transfer-Blot system (Bio-rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein extracts were resolved on SDS-PAGE (10% acrylamide) and transferred to a nitrocellulose Hybond ECL membrane (GE Healthcare, Pittsburgh, PA, USA) for probing with high-affinity rat monoclonal antibodies anti-HA (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... Membrane imaging was carried out with an ImageQuant LAS 4000 or an ImageQuant 800 luminescent imager (GE Healthcare Life Sciences, Piscataway, NJ). Rubisco loading control was stained using Ponceau S (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... The membrane was incubated with ECL™ Prime Western-Blot-Reagent (Cytiva,#RPN2236) and bands detected with an Amersham Imager 680 (GE Healthcare).
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bioRxiv - Cell Biology 2024Quote: ... For western blots protein samples from 1 x 106 trypanosome cells were separated on 12.5 % sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). For protein dot blots ...
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bioRxiv - Immunology 2024Quote: ... subjected to 10% SDS-PAGE using Laemmli discontinuous buffer system (59) and transferred into nitrocellulose membranes (GE Healthcare Life science, Buckinghamshire, UK). Staining with Ponceau S was used to verify loading and transfer efficiency ...
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bioRxiv - Evolutionary Biology 2021Quote: ... or with a GFX PCR DNA kit and Gel Band Purification kit (GE Healthcare, USA) following manufacturer guidelines ...
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bioRxiv - Biophysics 2020Quote: Surface plasmon resonance (SPR) assays were performed on Biacore S200 with a CM5 sensor chip (GE Healthcare Life Sciences) at room temperature ...
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bioRxiv - Biochemistry 2021Quote: The interaction between PttP1 and PttP2 was studied by a pulldown assay using His SpinTrap™ columns (GE Healthcare) with a bed volume of 200 μl ...
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bioRxiv - Biochemistry 2024Quote: ... Both tagged and cleaved BtuG samples were then loaded on a NAP-10 desalting column (kinetic assays, GE healthcare) for buffer exchange to 20 mM Tris/HCl pH 7.5 and 100 mM NaCl ...
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bioRxiv - Plant Biology 2020Quote: ... Seeds were sown in a prescribed seed pocket of the chip and the chips were put on the Hybond-N+ nylon membrane (GE Healthcare, Chicago, USA) which was placed on the 1/2 MS medium containing 0.5% sucrose and 1% agar ...
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bioRxiv - Cell Biology 2020Quote: ... The membranes were washed extensively with TBS-T before chemiluminescent detection using the ECL western Blotting Detection Reagents (GE Healthcare Life Sciences, RPN2106) and X-ray film or VersaDoc system (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... After washing the membranes with TBST, protein bands were detected with a chemiluminescent substrate (BIOMAX, USA) through a CCD Imager (GE life sciences, USA).
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bioRxiv - Genetics 2021Quote: ... All digested DNA was separated by electrophoresis on a 1% TBE agarose gel and subsequently transferred by capillary transfer to an Amersham Hybond-N+ nylon membrane (GE Healthcare, Milwaukee, WI).
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bioRxiv - Biochemistry 2020Quote: ... The primary antibody solution was dumped and the membrane washed three times for 10 minutes each with 1xTBST before adding the polyclonal Anti-Rabbit IgG Peroxidase (GE Healthcare; NA9340V; 16908235) at a 1:5000 dilution in milk ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were separated by SDS-polyacrylamide gel electrophoresis and transferred at 4 °C for 16 hours to a nitrocellulose membrane (Ø 0.2 µm GE Healthcare, Whatman, 10401396) through a wet transfer system (transfer buffer ...
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bioRxiv - Biochemistry 2021Quote: Extracted proteins of three-day-old sexual fungal tissues were separated by PAGE and transferred onto a nitrocellulose membrane (GE Healthcare, Wisconsin, USA) as described (Schinke et al. ...
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bioRxiv - Neuroscience 2020Quote: ... and 25μg of whole protein lysate were resolved on SDS-PAGE gels prior to transfer to PVDF membranes (GE Healthcare Life Sciences, #10600023) using a tank blotting apparatus (BioRad).
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Proteins were separated on 10–12 % SDS-polyacrylamide gel electrophoresis gel (SDS-PAGE; Carl Roth) and transferred to nitrocellulose membranes (GE Healthcare Life Science). Membranes were blocked for 1 h with 5 % non-fat milk in PBS-T (PBS with 0.1 % Tween-20 ...
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bioRxiv - Biophysics 2020Quote: ... Protein separation was carried out by SDS-PAGE in 10% bis-acrylamide gels and transferred to nitrocellulose membranes (Amersham Protran; pore size, 0.45 μm; GE Healthcare, Chicago, IL, USA). The membranes were blocked with 5% fat free milk in Tris-buffered saline (TBS ...
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bioRxiv - Cell Biology 2021Quote: ... UK)-treated PVDF membrane were then captured with a CCD camera (Lumino Imaging Analyzer, FAS-1100, TOYOBO; Amersham ImageQuant 800, GE Healthcare, Uppsala, Sweden).
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bioRxiv - Molecular Biology 2022Quote: ... Gels were blot-transferred to a nylon membrane (Amersham Hybond-N+ #RPN203B) and probed with pBR322 DNA sequences labelled with AlkPhos Direct (GE Healthcare® #GERPN3680). Chemiluminescent signals of increasing exposition periods were recorded with a cooled CCD camera (KODAK Gel Logic 1500 Imaging System ...
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bioRxiv - Molecular Biology 2022Quote: ... The RNA in the gel was transferred overnight to a nitrocellulose membrane with a positive charge (GE healthcare Amersham™ Hybond™ -N+) by capillary transfer using an assembly of Whatman® filter paper ...
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bioRxiv - Microbiology 2022Quote: ... the membrane was incubated with a self-made developing solution and protein levels were detected using an ImageQuant LAS 500 (GE Healthcare Life Sciences). Images were processed and band intensities quantified using ImageJ (37).
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bioRxiv - Plant Biology 2021Quote: ... 5 μg total RNA from Col and ktn1-20 was resolved on 15% Urea-PAGE gels and transferred onto Hybond NX membranes (GE Healthcare Life Sciences) followed by chemical cross-linking using EDC (1-ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Neuroscience 2020Quote: ... Immunoreactive bands on the membranes were visualized with a chemiluminescent reagent (ECL+) using a high performance chemiluminescence film (both from: GE Healthcare, Piscataway, NJ). Equal loading and transfer of proteins was verified with a reversible protein stain (Ponceaus S ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using 0.45 micrometer membranes (PES, ClearLine) and the protein purification was carried out by IMAC using 1 mL His-Trap FF columns (GE Healthcare Life Sciences) and a NGC chromatography system (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... Equal amounts of protein (10–50 μg) from the total extract were separated on 8–10% SDS–polyacrylamide gels and transferred onto nitrocellulose blotting membranes (GE Healthcare, Chicago, IL) at 12 V for 20 min in a semi-dry Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: Protein samples were separated by standard Laemli SDS-PAGE on 9% Tris-Glycine gels and transferred onto nitrocellulose membrane (GE Healthcare Bio-Sciences). PhosTag gels were prepared by adding 200 μM PhosTag™ (WAKO chemicals ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were revealed using Amersham ECL Prime (Cytiva) chemiluminescent detection reagent and imaged using an ImageQuant LAS 500TM imager (GE Healthcare Life Sciences).
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bioRxiv - Microbiology 2021Quote: ... membranes were incubated with horseradish peroxidase-coupled secondary antibodies and imaged using chemiluminescent substrate on an Amersham Imager 600 (GE Healthcare, Chicago, Illinois).
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bioRxiv - Microbiology 2020Quote: ... and same protein quantities were separated on a 10% SDS-PAGE gel under reducing conditions and transferred to 0.45 μm PVDF blotting membrane (GE healthcare, Chicago, IL, USA). Total ERK1/2 and phospho-ERK1/2 were detected using p44/42 MAPK antibody (1:1000 in 5% BSA/TBS-T ...
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bioRxiv - Microbiology 2021Quote: Whole cells lysate (50 µg) was separated on 12% SDS-PAGE and then transferred onto a PVDF membrane (GE Healthcare, Piscataway, NJ, USA). Membrane were blocked in 5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... Quantification of band intensities was carried out on digital images of membrane samples provided by LAS4000 gel documentation system (GE Healthcare; Barcelona, Spain) using ImageJ software (1.50b ...
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bioRxiv - Neuroscience 2021Quote: ... plasma samples were centrifuged and 1 μL of each sample was placed onto a clean piece of nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, USA) and left to dry out ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... of prepared protein samples were loaded and separated on 10% sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene fluoride (PVDF) membrane (GE Healthcare, Buckinghamshire, UK). NZYColour Protein Marker II (NZYTech ...
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bioRxiv - Cell Biology 2021Quote: ... and 25µg of whole protein lysate were resolved on SDS-PAGE gels prior to transfer to PVDF membranes (GE Healthcare Life Sciences, #10600023) using a tank blotting apparatus (BioRad).
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bioRxiv - Bioengineering 2021Quote: ... The egg rafts were disaggregated and aligned horizontally on a piece of moistened chromatography paper and against a nitrocellulose membrane (GE Healthcare, Amersham UK). Lines of embryos were then transferred to a piece of Scotch double-sided tape 665 (3M ...
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bioRxiv - Cell Biology 2022Quote: ... 20 μg total protein for each condition was resolved on 12% SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare Life Sciences) at 350 mA for 3 h for blotting ...
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bioRxiv - Physiology 2022Quote: ... and ran this mixture through handcast Tris-glycine gels prior to blotting onto nitrocellulose membranes with 0.1 µm pore size (GE Healthcare, Chicago, IL, US). Subsequently ...
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bioRxiv - Plant Biology 2022Quote: ... Twenty micrograms of total RNAs were separated on 15% acrylamide/8 M urea gel and transferred onto an Amersham™ Hybond™-N+ nylon membrane (GE Healthcare). The specific isotope probe was generated by end-labeling DNA oligonucleotides complementary to the miR167a sequence with [γ-32P] ATP by T4 polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Bioengineering 2024Quote: ... 2 μg DNA loaded per lane was separated by electrophoresis on 1% agarose gels and transferred to Hybond-N membranes (GE Healthcare, Chicago, IL) using capillary flow transfer method ...
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bioRxiv - Cancer Biology 2024Quote: ... Twenty micrograms of total protein were loaded either on a 12% polyacrylamide-SDS gel and transferred to a 0.2 µm nitrocellulose membrane (No10600001, GE Healthcare; Chicago, IL, USA). Membranes were blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: WB was performed on a semi-dry western blot device transferred to an Amersham Protran 0.45 mm nitrocellulose (NC) blotting membrane (GE Healthcare, Chicago, IL, USA) at 52 mA for 45 min ...