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Citations for GE Life Sciences :
1551 - 1600 of 2545 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were washed five times and chemiluminescence was detected with ECL Prime Western Blotting Detection Reagents (Amersham, GE Healthcare). The signal was quantified with ImageJ software.
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bioRxiv - Developmental Biology 2020Quote: ... the membranes were washed 4 times in TBS-T and then incubated with secondary antibodies for 1h (1:4,000; GE Healthcare) and washed 3 times with TBS-T ...
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bioRxiv - Physiology 2022Quote: ... The membrane was incubated for 1 hour at RT in 2% enhanced chemiluminiscence (ECL) prime blocking agent (GE Healthcare, RPN418). Membranes were incubated overnight at 4⁰C in blocking buffer (4% bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2020Quote: ... the same gel was electroblotted to a PVDF membrane (0.2 µm) and the immunodetection was performed using enhanced chemiluminescence (ECL; GE Healthcare). Anti-NAB1 antiserum was generated as described (Mussgnug et al. ...
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bioRxiv - Genomics 2021Quote: ... Samples were separated in a 10% acrylamide (Roth, Karlsruhe, Germany) gel and blotted onto nitrocellulose membrane (GE Healthcare, Freiburg, Germany) in a wet system ...
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bioRxiv - Developmental Biology 2020Quote: ... the supernatant was boiled at 98 °C for 5 min, applied to SDS page gel (SuperSep, WAKO) and transferred onto a 0.2 µM nitrocellulose blotting membrane (GE Healthcare). After incubation with blocking buffer (5% milk in tris-buffered saline with 0.1% Tween20 ...
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bioRxiv - Microbiology 2021Quote: ... samples were separated on an 0.8% agarose-TAE gel and transferred onto nylon membranes using a Turbo Blotter (GE Healthcare). Membranes were UV cross-linked (120 mJ/cm2) ...
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bioRxiv - Biochemistry 2020Quote: ... 100 pmoles of DNA samples were blotted on a positively charged nylon membrane (Amersham Hybond-N+, GE Healthcare Life Science). The DNA was then UV crosslinked to the membrane (254 nm ...
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bioRxiv - Biochemistry 2020Quote: Proteins were transferred from the SDS-PAGE gel to a 0.2 μm Amersham Protran Nitrocellulose blotting membrane (GE life sciences) using a BioRad Trans-blot cell gel tank filled with transfer buffer (192 mM glycine ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 hour at 30V onto Amersham Hybond P 0.45 PVDF membranes (GE Life Sciences, Pittsburgh, PA; cat # GE 10600029). After transfer membranes were rinsed in TBST and blocked for one hour in either 5% milk (PERK ...
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bioRxiv - Cell Biology 2021Quote: ... 20% of each sample was loaded and resolved on a 10% SDS-PAGE gel and transferred to 0.2-μm nitrocellulose membranes (GE Healthcare). Membranes rinsed in PBS/0.1% Tween-20 (PBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... The protein samples were resolved on SDS-PAGE gels and then transferred to nitrocellulose blotting membranes (GE Healthcare Life Sciences).
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bioRxiv - Plant Biology 2020Quote: ... An equal amount of protein was boiled in Laemmli buffer for 10 min at 95°C and separated on 12.5% acrylamide gel and blotted on a nitrocellulose membrane (GE healthcare). Blocking was done with 0.5% w/v of BSA in Tris-buffered saline with 0.1% v/v Tween 20 (TBST) ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated with the secondary antibody (HRP-conjugated anti-mouse or anti-rabbit IgG antibody, GE Healthcare; 1:2000) in TBST plus 2% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μg of total RNA from each sample was analysed through 1.2% agarose-formaldehyde gels and transferred to HybondTM-N+ membrane (GE Healthcare) by neutral transfer ...
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bioRxiv - Neuroscience 2020Quote: ... 1:20,000-1:40,000),immunoreactive bands on the membranes were detected by enhanced chemiluminescence (ECL Plus; GE Healthcare Bio-Sciences). For total protein expression ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed 3 times for 5 min each with 1X TBST buffer and developed by chemiluminescence (GE healthcare, RPN2106). The Odyssey FC imaging system (LI-COR ...
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bioRxiv - Cell Biology 2021Quote: ... followed by transfer of proteins from gel to polyvinylidene fluoride (PVDF) membrane (Amersham Hybond P 0.45, GE Healthcare Life Sciences) and sequential use of primary and secondary antibodies that are listed in Table 3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lysates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (GE Healthcare). After blocking with 8% skim milk or 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were electroblotted to 0.45μm PVDF membranes (Imobilon-P, Merck Millipore, IPVH00010; or Amersham Hybond, GE Healthcare Life Science, 10600023), and immunodetected using primary antibodies directed against phospho-Ser935 LRRK2 rabbit monoclonal (1/1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant of the lysate was filtered through a 0.22 µm membrane filter and loaded onto a Ni-NTA column (HiTrap, 5 ml, GE Healthcare) using a low imidazole buffer (Buffer C ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysate was clarified by centrifugation at 35000 g for 30 minutes at 4° C and the supernatant was passed through a 0.45 µm membrane filter before loading onto a pre-equilibrated Strep Trap HP column (GE Healthcare) equilibrated previously with Strep Trap buffer A (50 mM Tris pH 8.5 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins were separated using standard SDS-PAGE electrophoresis techniques and transferred onto 0.45 μm nitrocellulose membranes (GE Healthcare, Amersham, U.K.) where non-specific binding was blocked by 5% (w:v ...
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bioRxiv - Microbiology 2022Quote: ... Transfer was performed in an XCell II Blot Module in NuPAGE Transfer Buffer (Thermo Fischer) onto a Hybond P 0.45 PVDF membrane (GE Healthcare). After blocking in 5% BSA or milk powder (Carl Roth ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were electrophoretically separated on an 8% polyacrylamide gel and then wet- transferred to a nitrocellulose membrane (Amersham Protran 0.2 NC, GE Healthcare). Prior to antibody incubation ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated with either 1 ml of Amersham ECL substrate western blotting detection reagent (GE Healthcare lot# 9622301) or for higher sensitivity ...
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bioRxiv - Microbiology 2020Quote: ... The gels were destained with ultra-pure water for 20 minutes and were transferred to Hydrobond-XL-membrane (GE healthcare) and electro transferred using 0.5X TAE as the transfer buffer for 45 minutes at 150 V ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated by SDS-PAGE on a 6% gel and transferred to a nitrocellulose membrane (0.45 µm pore size, GE Healthcare) by Trans-Blot Turbo system (BioRad) ...
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bioRxiv - Plant Biology 2021Quote: ... total RNA (1 – 10 μg) was separated using formaldehyde (2.2 M) gel electrophoresis and transferred to a Hybond-NX Nylon membrane (GE Healthcare) via capillary transfer ...
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bioRxiv - Microbiology 2021Quote: ... After gentle stirring for one hour aggregates were removed by centrifugation at 75,600g and the solubilised membrane proteins were applied to a StrepTrap column (GE healthcare) which was then washed in TBS supplemented with 0.01% LMNG ...
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bioRxiv - Cancer Biology 2020Quote: ... membranes were incubated with the appropriate horseradish peroxidase (HRP)-labeled secondary antibody (1:5000 dilution; GE Healthcare UK Ltd, UK). Protein bands were detected using enhanced chemiluminescence (Luminata Crescendo ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered through a 0.45-μm membrane and loaded onto a HisTrap FF 5 mL column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... 30 μg of total protein lysate was resolved on SDS polyacrylamide gel according to standard procedure at 20 mA per gel and blotted onto a nitrocellulose membrane 0.45 μm (GE Healthcare) via Mini Trans-blot module (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... rolling circle replication products created in the absence of [α-32P]-dATP were separated in a 0.6% alkaline denaturing agarose gel and transferred to a Nylon membrane (Hybond – XL, GE Healthcare) by a downward method after mildly cleaved in 0.25 M HCl ...
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bioRxiv - Immunology 2022Quote: ... The membrane was developed with the ImmunoStar LD enhanced chemiluminescence reagents (Wako) and visualized using ImageQuant LAS 400 (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... Bio-Rad (4–15%) gradient gels were used for gel electrophoresis Proteins were blotted onto 0.2 µm nitrocellulose membranes (Amersham Protran, GE Healthcare) using the Thermo Scientific Pierce G2 Fast Blotter ...
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bioRxiv - Zoology 2020Quote: The SDS-PAGE gel was transferred to 0.45µm pore nitrocellulose (NC) membrane (GE Life Sciences #10600003 Protran Premium 0.45 NC) using the AA-Hoefer TE22 Mighty Small Transfer unit at 110V for 90 minutes with water cooling and immersion in an icebath ...
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bioRxiv - Immunology 2020Quote: ... the membrane was processed for detection of peroxidase activity with chemiluminescence reagents and an Image Quant LAS4000 instrument (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... 20 mM imidazole pH 7.5 was added and the solubilized membranes were loaded on a pre-equilibrated 5 ml Ni-NTA column (GE Healthcare) at 3 ml/minute ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were further incubated with horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (1:3,000; GE Healthcare, USA) in PBST-BSA for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Total proteins were separated in a 4–15% SDS–PAGE gradient and transferred to nitrocellulose membrane (GE Healthcare Life Science). Membranes were blocked with 5% (m/v ...
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bioRxiv - Immunology 2020Quote: ... and transferred by western blotting (30V, 1 hour) to polyvinylidene fluoride membrane (P 0.45 μm; 10600023 Amersham Hybond, GE Healthcare). Blots were probed with biotinylated GNA lectin (40 μg/ml ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Membranes were developed using ECL and images were obtained with ImageQuant LAS 4000 camera system (GE Healthcare, Chicago, IL, USA). Band intensities were quantified using ImageJ software (NIH).
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bioRxiv - Biochemistry 2021Quote: ... then the soluble fraction was filtered through 0.22 μm PVDF membrane before loading onto a 5 mL HisTrap HP column (GE Healthcare). The column was washed with 20 column volumes of 50 mM Tris-HCl pH 7.0 ...
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bioRxiv - Biophysics 2020Quote: ... The soluble fraction was filtered through a 0.22 μm membrane before being loaded on a HiScreen Capto Q ImpRes ion exchange column (GE Healthcare). The AβM(E22G)-mCherry was eluted from the column over seven column volumes on a linear gradient against IEX buffer B (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... genomic DNA from WT-GFP and mutant parasites was digested with HindIII and fractionated on a 0.8% agarose gel before blotting onto a nylon membrane (GE Healthcare). A probe was generated from a PCR fragment homologous to the 3’ sequence just outside of the targeted region using the AlkPhos direct labelling kit (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were soaked with 1:5000-diluted HRP-conjugated anti-rabbit or anti-mouse IgG secondary antibodies (GE Healthcare) in TBST containing 5% skim milk for 30 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting supernatant was filtered through 0.2 µm filter membranes prior to loading onto a Tricorn 10/20 column (GE Healthcare) packed with 2 ml amylose resin (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: Proteins (10 μg) were separated by 10%–12.5% SDS-PAGE and electroblotted onto a 45-μm nitrocellulose membrane (GE Healthcare). Membranes were stained with Ponceau to ensure equal distribution of proteins in each lane ...
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bioRxiv - Immunology 2020Quote: ... with a 3 kDa membrane cutoff and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare Life Sciences) size exclusion chromatography column previously equilibrated with 7M urea ...