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Citations for GE Life Sciences :
1451 - 1500 of 2545 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: RNA samples separated by 11% WIDE Range Gel SDS-PAGE were transferred onto a Hybond-N+ membrane (GE Healthcare) and hybridized with biotinylated oligonucleotide(s ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were separated by SDS-PAGE and transferred to AmershamTM ProtranTM 0.45 μm nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were probed with primary antibodies at dilutions recommended by the suppliers ...
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bioRxiv - Molecular Biology 2020Quote: ... The DNA was then transferred by capillarity in SSC 20X onto an Amersham Hybond-X membrane (GE healthcare RPN203). For TTAGGG repeats probe the 800 bp EcoRI fragment of the Sty11 plasmid (a gift from Titia de Lange ...
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred from polyacrylamide to nitrocellulose membranes (AmershamTM ProtranTM Premium 0.45 µm NC, GE Healthcare Life sciences, 10600003) by electrotransfer (Bio-Rad laboratories Inc.) ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were incubated with ECL reagent (Thermo) and signal was detected using an Amersham imager 600 device (GE healthcare). Images were processed using ImageJ software (National Institutes of Health).
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... and proteins were separated by SDS-PAGE gel electrophoresis and transferred to a PVDF membrane (GE Healthcare Life Sciences). The following antibodies were used ...
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bioRxiv - Genetics 2020Quote: ... Samples were separated on 10–12% SDS-PAGE gels and proteins were transferred to Protran nitrocellulose membranes (GE Healthcare), after which membranes were stained with 0.3% w/v Ponceau S in 3% v/v acetic acid and digitized ...
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bioRxiv - Developmental Biology 2021Quote: ... DNA fragments were electrophoresed in 0.8% agarose gel and transferred to an Amersham Hybond XL membrane (GE Healthcare, RPN303S). The b-geo probe was designed downstream of the BamHI site ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were then separated by SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose Amersham Protran membrane (GE Healthcare). Membranes were blocked at room temperature with either 5% (w/v ...
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bioRxiv - Synthetic Biology 2021Quote: ... Digested DNA was electrophoresed in 1% agarose and transferred by capillarity onto a nylon membrane (Hybond N+, GE healthcare). Membranes were hybridized in Church buffer at 68°C with two different 32P-radiolabeled probes (Church and Gilbert ...
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bioRxiv - Microbiology 2021Quote: ... the gels were blotted onto polyvinylidene difluoride (PVDF) membranes (Amersham™ Hybond™, 0.45 nm, GE HealthCare Life Science) at 60 mA for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed using 0.1% TBS-T and proteins were detected using ECL or ECL Select (GE Healthcare, USA) on ChemiDoc XRS+ System (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... The medium from 1 L of culture was filtered through a 0.22-μm membrane and incubated with 3.0 mL of Ni-Sepharose Excel (Cat 17-3712-03, GE Healthcare) in the presence of 20 mM of imidazole for 2-3 h at 4 °C with mild agitation ...
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bioRxiv - Cancer Biology 2020Quote: ... and 40-80 µg protein was used for western blotting (AmershamTM HybondTM - ECL nitrocellulose membranes, GE Healthcare, Chicago, IL) with a wet-transfer system (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were then washed five times for 5 min and proteins were detected with Amersham ECL (GE Healthcare). Images were taken using the iBright Imaging System (Invitrogen).
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bioRxiv - Plant Biology 2021Quote: ... separated by gel electrophoresis in 0.7% (w/v) agarose gels and blotted onto Hybond N nylon membranes (GE Healthcare). A ~500 bp probe was produced by PCR amplification (primer pair P19/P20 ...
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bioRxiv - Microbiology 2020Quote: ... Protein lysates were obtained by lysing infected cell monolayers in 4x Laemmli sample buffer and were analysed by semi-dry Western blotting onto Hybond 0.2µM polyvinylidene difluoride (PVDF) membrane (GE Healthcare). Membranes were incubated with rabbit antisera diluted in PBS with 0.05% Tween-20 containing 5% dry milk (Campina) ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were then separated using a 7.5% SDS-PAGE gel and transferred to a nitrocellulose membrane (GE healthcare Lifesciences ...
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bioRxiv - Microbiology 2020Quote: ... Chromosomal DNA fragments were separated on a 1% agarose gel and blotted onto Hybond N+ nylon membranes (GE Healthcare), following standard techniques (Sambrook and Russell ...
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were then blocked for 1 h with TBS-T 1% ECL Prime blocking reagent (GE Healthcare cat # RPN418) at RT on an orbital shaker and blotted with primary antibody for 1 h at RT with gentle agitation ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were visualised on an Odyssey CLx Imaging System (LiCOR) (Figure 1C) or an ImageQuant LAS 4000 (GE Healthcare) with SuperSignal West Pico PLUS HRP substrate (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to nitrocellulose membranes (Amersham™ Protran™ Premium 0.45 μm NC, GE Healthcare Life sciences, 10600003). For Msb2p-HA and Hkr1p-HA blots ...
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bioRxiv - Cancer Biology 2020Quote: ... All protein lysates were run on NuPage 4–12% gradient gels (LifeTechnology) and transferred to PVDF membrane (GE Healthcare). Membranes were blocked in 5% milk in TBST and stained with primary antibody overnight at +4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The digested DNA was electrophoresed on a 0.8% agarose gel and transferred to a Hybond-N+ membrane (GE Healthcare). The membrane was hybridized with a digoxigenin-labeled DNA probe generated with a PCR DIG Probe Synthesis Kit (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were then transferred onto a nitrocellulose membrane (Amersham™ Protran™ 0.2 µm NC, GE Healthcare Life science) using a standard blotting procedure (80V for 2h ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were directly placed in a slot on a nylon membrane by vacuum filtration (Amersham Hybond-XL-GE Healthcare) using a transfer collector (PR648-Hoefer ™ Slot Blot) ...
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bioRxiv - Cancer Biology 2022Quote: ... 25µg of protein were resolved on sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under denaturing conditions and transferred to Protran Premium NC 0.45µm membranes (Amersham Biosciences, GE Healthcare). Membranes were blocked for 1h at RT and incubated overnight at 4°C with agitation ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were resolved by SDS-PAGE using 15% gels and transferred onto nitrocellulose membranes (Amersham Protran, GE Healthcare, USA). Immunoblots were processed by enhanced chemiluminescence (ECL ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were exposed to storage phosphor screens before scanning on the Typhoon FLA 9500 system (GE Healthcare Life Sciences). To strip membranes prior to re-probing for different transcripts ...
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bioRxiv - Microbiology 2020Quote: SΔTM and RBD glycoproteins were resolved on SDS-PAGE and transferred onto a nitrocellulose membrane (Amersham Protran, GE Healthcare). Lysates of 293T cells transfected with plasmids encoding full-length spike glycoproteins was also separated by SDS-PAGE for WB ...
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bioRxiv - Microbiology 2020Quote: ... Anti-rabbit IgG HRP-linked secondary antibody and 0.45-μm polyvinylidene difluoride (PVDF) membrane were obtained from GE healthcare. Specific-Pathogen-Free (SPF ...
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bioRxiv - Developmental Biology 2020Quote: ... and then transferred onto nitrocellulose membrane Watman® Optitran® BA-S 85 (GE Healthcare, Castle Hill, NSW, Australia). The membrane was blocked (1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... or denaturing 0.8% agarose gel for large RNA separation (0.2 to 3 kb) and transferred onto membrane Hybond N+ (GE Healthcare). RNAs were probed with γ32P 5’ end-labeled oligonucleotide (Table S3 ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was collected and filtered using 0.22µm membrane filters and loaded onto HiTrap MabSelect SuRe Protein A (GE Healthcare) column pre-equilibrated against PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Extracts (15⍰μg protein) were separated by SDS–PAGE and transferred to nitrocellulose membranes (GE Healthcare, Chicago, IL, USA). Membranes were blocked in TBS (150⍰mM NaCl ...
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bioRxiv - Immunology 2020Quote: ... acid-eluted immunoprecipitates were separated by SDS-PAGE under reducing conditions and transferred to HybondTM ECLTM membranes (GE Healthcare). Membranes were incubated sequentially with the different primary antibodies and developed with the appropriate HRP-conjugated secondary antibody using enhanced chemiluminescence (ECL) ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were filtered through a 0.45-μm membrane and loaded onto a HisTrap FF 5 mL column (GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.6 M NaCl before transfer under neutral conditions on Amersham™ Hybond-XL nitrocellulose membrane (GE Healthcare, Cat# RPN203S). After one round of UV auto-crosslink (120,000 μJ/cm2 ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were typically resolved on 7.5% TGX stain-free gels and transferred onto nitrocellulose membrane (GE Healthcare, Amersham Protran) using wet-transfer at 100 V for 60 min ...
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bioRxiv - Genetics 2022Quote: ... total RNA samples were resolved in 15% polyacrylamide/7M urea gels and electroblotted to Hybond-XL membranes (GE Healthcare) (Ibrahim et al. ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated on a 7.5% Bis-Tris gel and blotted onto a 0.45 µm nitrocellulose membrane (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... protein extracts were resolved by 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (#10600021, GE Healthcare).
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bioRxiv - Physiology 2021Quote: ... membranes were incubated for 5 min at room temperature with the Amersham ECL Select peroxidase substrate (RPN2235; GE healthcare), and imaged with the Fusion camera ...
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bioRxiv - Genetics 2020Quote: ... The membrane was exposed to a phosphoimager screen and the signal was detected via Typhoon FLA 9500 (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... Twenty to 30 μg of proteins were separated by SDS-PAGE and then transferred to nitrocellulose membranes (GE Healthcare). Monoclonal anti-glutathione peroxidase 1 (GPx1 ...
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bioRxiv - Molecular Biology 2020Quote: ... The blot membrane was subjected to densitometric scanning and the band intensities were quantified using ImageQuant TL (GE Healthcare). The primary antibodies used in this study are as follows ...
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bioRxiv - Cell Biology 2021Quote: ... a phosphor screen was exposed with the membrane and was subsequently scanned using a Typhoon FLA 9000 (GE Healthcare). Signals were measured with the Fiji software (Schindelin et al. ...
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bioRxiv - Immunology 2021Quote: ... the membranes were washed three times in TBST and incubated for 5 minutes with a chemiluminescent reagent (GE Healthcare) to visualize immuno-reactive bands ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was then incubated with increasing amounts of ribosomal subunits/ribosomes for 15 min at 37°C before being rapidly filtrated through a cellulose nitrate membrane filter (0.2μm, GE Healthcare). The cellulose nitrate membranes were washed with 1 mL of cold TAKM5 buffer and placed into 10 mL of EcoLite (+ ...
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bioRxiv - Neuroscience 2021Quote: ... 25ug or 5ug of protein extracts were separated under denaturating conditions in 12% SDS-PAGE gels and transferred to nitrocellulose membranes (0.45μm GE HealthCare) for 2 hours ...