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Citations for GE Life Sciences :
1851 - 1900 of 2545 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... at 25 mA for 1 h and transferred onto an Amersham™ Protran® 0.2 μm nitrocellulose blotting membrane (GE Healthcare, Canada) at 300 mA for 1.5 h ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was developed with an ECL reagent (Amersham ECL™ Prime Western Blotting detection reagent from GE Healthcare UK, Cat# RPN2232) and scanned by FluorChem™ 8900 (Alpha Innotech ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were separated by SDS-PAGE and then transferred to nitrocellulose membranes (Amersham™ Protran™ Premium 0.45μm 300mm × 4m, GE Healthcare, USA). Membranes were blocked for 1 hour using 3% BSA (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... HRP-conjugated secondary antibodies were incubated for 1 h at room temperature and membrane development was performed using Enhanced Chemiluminescence (ECL) Western Blotting substrate (GE healthcare, RPN2106) or ECL Prime Western Blotting substrate (GE healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... was used for imaging and densitometry analysis after membranes were incubated with enhanced chemiluminescence (ECL™) western blotting detection reagents (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... The samples were separated by a 10% SDS-PAGE and transferred to a nitrocellulose membrane (Amersham Protran 0.45 μm NC; GE Healthcare Life Science). The membranes were blocked in 1x TBS-0.1% Tween-20 (TBS-T ...
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bioRxiv - Molecular Biology 2022Quote: ... The lysate was clarified by centrifugation at 35000 g for 30 minutes at 4° C and the supernatant was passed through a 0.45 µ membrane filter before loading onto a pre-equilibrated HiTrap immobilized metal ion affinity (IMAC) column (HiTrap IMAC FF, GE Healthcare). The column was washed extensively with wash buffer (20 mM Tris pH 7.9 ...
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bioRxiv - Biochemistry 2022Quote: ... 4 μg of total RNA was resolved on a denaturing 1% agarose/1.25% formaldehyde gel using Tri/Tri buffer (Mansour and Pestov 2013) and transferred to a Hybond-XL membrane (GE Healthcare RPN 303S). Blots were hybridized to radiolabeled DNA oligonucleotide probes (P4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Approximately 10 μg of total protein was separated by SDS-PAGE electrophoresis and transferred to a nitrocellulose membrane with either 0.4 μm pores (GE Healthcare, Chicago, IL) or 0.2 μm pores (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... filtered through a 0.22 μm membrane filter and loaded onto a 5 ml HiTrap Heparin HP column (GE Healthcare, Chicago, Illinois, USA). The column was washed with 10 volumes of Buffer H (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... 10 μl of samples were loaded on 12% SDS-PAGE and transferred to Amersham Protran premium 0.2 μm nitrocellulose membranes (GE Healthcare Life Sciences) for 1 hour in an ice-cold transfer buffer (25mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Reduced proteins were run on 8%,10% or 12% SDS-PAGE gels and transferred on to 0.22 μm nitrocellulose membranes (GE Healthcare Life Sciences). Membranes were blocked using 5% skim milk in 1X Tris-buffered saline and 0.1% Tween 20 (1XTBST ...
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bioRxiv - Neuroscience 2022Quote: ... Homogenates were run in polyacrylamide gels (Bio-Rad, Hercules, California, USA and transferred to BioTraceTM Nitrocellulose membranes (GE healthcare, Chicago, Illinois, USA). Membranes were blocked for 1h in 5% skim milk in TBS-Tween-20 (TBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... + RNA was first denatured by heating at 65°C for 5 minutes and then transferred to A cellulose nitrate membrane (Amersham, GE Healthcare, US) using A Bio-dot device (US Bio-RAD).The membranes were then uV-crosslinked ...
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bioRxiv - Plant Biology 2020Quote: ... Visualization of the stained protein on nitrocellulose membranes was carried out by a LAS-4000 Mini luminescence image analyzer (GE Healthcare, Germany) using the ECL Western Blotting Detection Reagents (GE Healthcare ...
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bioRxiv - Plant Biology 2020Quote: ... Seeds were sown on the devices and the devices were put on the Hybond-N+ nylon membrane (GE Healthcare, Chicago, IL, USA) which was placed on 1/2 MS medium supplemented with 0.5% sucrose and 1% agar ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and the supernatants were filtered through a polytetrafluoroethylene (PTFE) membrane syringe filter (pore size of 0.2 μm, 13 mm of diameter, GE Healthcare Life Sciences). The relative disappearance of the compound under study over the course of the incubation was monitored by HPLC-MS using SIM mode ...
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bioRxiv - Neuroscience 2021Quote: ... 20 ug of protein extracts for analysis of MT severing enzymes or 2.5 ug of protein extracts for analysis of tubulin proteins were separated under denaturing conditions and transferred to Amersham Protran Premium 0.45 μm nitrocellulose membranes (GE Healthcare Life Sciences) prior to blocking in 5% non-fat dried milk in TBS-T for 1 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... isolated E coli membranes with overexpressed MgtA were solubilized in 1% β-dodecyl maltoside (β-DDM) prior to Histrap HP (GE Healthcare) purification equilibrated in (25 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2021Quote: ... antibody (72) and the membranes were washed with PBS and incubated with horseradish peroxidase (HRP)-conjugated donkey anti-rabbit (GE Healthcare, #NA934V) and/or sheep anti-mouse (GE Healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were rinsed in PBS + 0.05% Tween-20 and visualized by ECL in an ImageQuant LAS 4000mini (GE Healthcare, Chicago, IL USA) as above.
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bioRxiv - Microbiology 2020Quote: ... Intracellular Gus and LacZ activity was visualized by placing a nitrocellulose membrane (Amersham TM Protran TM 0.45 μM NC, GE Healthcare Life Sciences) on top for 24 h at 28 °C ...
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bioRxiv - Immunology 2021Quote: ... denatured in Denaturation buffer [0.5N NaOH and 1.5M NaCl (Fisher)] for 30 min with gentle agitation and then transferred to Hybond-XL membranes (GE Healthcare Amersham/Fisher). Synthesized DNA specific to regions of homologous recombination (see Oligonuleotide section in Key Resources Table ...
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bioRxiv - Cell Biology 2021Quote: ... Protein precipitates were analyzed by SDS-PAGE and transferred to a nitrocellulose membrane (Cat#10600003, AmershamTM ProtranTM Premium 0.45 μm NC, GE Healthcare Life sciences). Monoclonal mouse anti-GFP antibodies were used (Cat#11814460001 ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates or mitochondria samples were separated on 10 – 18 % Tris-Tricine gels and transferred onto Amersham™ Protran™ 0.2 µM nitrocellulose membranes (NC, GE Healthcare). For immunodetection ...
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bioRxiv - Cell Biology 2022Quote: ... cell lysates were resolved by SDS-PAGE and proteins were transferred to a nitrocellulose membrane (Cat#10600003, AmershamTM ProtranTM Premium 0.45 μm NC, GE Healthcare Life sciences) which were incubated in blocking buffer (5% nonfat dry milk ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 μg of total RNA was resolved on a denaturing 1% agarose/1.25% formaldehyde gel using Tri/Tri buffer (Mansour and Pestov 2013) and transferred to a Hybond-XL membrane (GE Healthcare, RPN 303S). UV-crosslinked membranes were stained with methylene blue (0.025% w/v ...
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bioRxiv - Synthetic Biology 2022Quote: ... The samples were run on the gel at 300V for 135 minutes using a gel transfer system (Doppel-Gelsystem Twin L, PerfectBlue).The samples were transferred to a Hybond-XL membrane (GE healthcare, RPN203S) at 50V for 1 hour at 4°C and then cross-linked using 0.12J (UV-lamp T8C ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μl of each mucus sample was pipetted onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA), left to dry out ...
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bioRxiv - Plant Biology 2022Quote: ... or adjusted to Chl/protein content were fractionated by electrophoresis on SDS-polyacrylamide gels (12%) and blotted onto nitrocellulose membranes (Amersham Protran, GE Healthcare, UK). Membranes were stained with Ponceau S and probed with protein-specific antibodies according to (Sambrook ...
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bioRxiv - Neuroscience 2022Quote: ... Equal amounts of protein samples were separated on 8% Tris/glycine gels and then transferred to nitrocellulose membranes (GE Healthcare, Uppsala, Sweden). Subsequently the membranes were incubated with primary antibodies and HRP-coupled secondary antibodies (Jackson ImmunoResearch ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 or 15 % 29:1 acryl:bis-acrylamide) and transferred on Amersham™ Protran™ 0.45 μm nitrocellulose membranes (GE Healthcare, Cat# 10600033) at 4°C for 1 hr at 110V ...
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bioRxiv - Neuroscience 2022Quote: ... The chemiluminescence was detected in Fusion FX7 image and analytics system (Vilber Lourmat) after treatment of the membrane with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences).
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bioRxiv - Neuroscience 2022Quote: ... samples were separated by a 12% acrylamide-bis acrylamide gel and transferred to a polyvinylidene fluoride (PVDF) membrane (GE Healthcare, IL, USA). Blocking was performed with 5% milk powder for 45 min at RT and incubated in the following antibodies at the indicated dilutions ...
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bioRxiv - Microbiology 2022Quote: ... microbial biomass – was collected by vacuum filtration onto 0.22 μm polycarbonate filters (GE Healthcare Whatman 47 mm Nucleopore Polycarbonate Track Etched Membranes; GE Healthcare 111106). All filtering was done inside a sterile laminar flow hood ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then subjected to SDS-PAGE followed by transfer of the proteins onto nitrocellulose membranes (GE Healthcare, Chalfont St Giles, UK). Membranes were blocked in 3% BSA/PBST (1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... were run on standard 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (pore size 0.45 μm, GE Healthcare Life Sciences). These were then blocked for 1 h with either a 3% BSA solution or 5% milk solution in Tris-buffered saline (20mM Tris ...
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bioRxiv - Systems Biology 2024Quote: ... by SDS-PAGE (10 %, 15 %, 90 µg) and transferred onto nitrocellulose membranes (0.2 µm) (GE Healthcare Life Sciences, Chalfont St Giles, UK) using the semi-dry TransBlot® Turbo™ transfer system (Bio-Rad Laboratories ...
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bioRxiv - Pathology 2024Quote: ... Proteins were separated by SDS-PAGE (10 % polyacrylamide gel) and transferred to a PVDF membrane (RNP303F, GE Healthcare, Little Chalfont, Buckinghamshire, UK) in a semi-dry blotter (TE70X ...
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bioRxiv - Genetics 2023Quote: ... analysis and were transferred from gels to a nitrocellulose membrane (Cat#10600003, Amersham Protran Premium 0.45 μm NC; GE Healthcare Life sciences). Membranes were probed with rabbit polyclonal p44/42 antibodies (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... 20 μg of the heat-denatured protein lysate of each sample was loaded on a 4–12% SDS-PAGE and then transferred via wet blotting onto a 0.45 μm nitro-cellulose membrane (GE Healthcare, Solingen, Germany). After 1 h of blocking with 5% milk in TBST ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were then washed in PBT and incubated with HRP conjugated-anti-rabbit IgG (GE Healthcare # NA9340, 1:5000 in blocking solution) at room temperature for 1 hour and washed twice in PBT ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was measured by Bradford assay and 20 µg of denatured protein (in Laemmli buffer at 95°C for 5 min) was loaded in a 7.5% polyacrylamide gel and blotted onto a nitrocellulose membrane (GE Healthcare, Buckinghamshire, UK). The membranes were blocked in 5% milk in PBS with 0.01% Tween (PBST ...
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bioRxiv - Biophysics 2023Quote: Proteoliposomes were thawed and extruded through a NucleporeTM Track-Etch Membrane polycarbonate filter with 400 nm pore size (GE Healthcare Life Siences). Uptake was assayed in a 96-well setup in ultra-low attachment ...
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bioRxiv - Cancer Biology 2023Quote: ... Imaging of membranes for chemiluminescence or after ponceau S staining was performed using the Amersham Imager 600 (GE HealthCare Life Sciences, USA). Quantification of protein expression was performed using ImageJ software ...
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bioRxiv - Plant Biology 2023Quote: ... Membrane imaging was carried out with an ImageQuant LAS 4000 or an ImageQuant 800 luminescent imager (GE Healthcare Life Sciences, Piscataway, NJ). Rubisco loading control was stained using Ponceau S (Sigma).
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bioRxiv - Neuroscience 2023Quote: Tissue lysates were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (GE Healthcare Life Sciences, Pittsburgh, PA).
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were diluted in Laemmli buffer and separated by SDS-PAGE before transfer onto polyvinylidene difluoride or nitrocellulose membranes (both GE Healthcare Science). After primary antibody incubation (Progranulin (clone 8H10 ...
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bioRxiv - Biochemistry 2023Quote: ... Protein precipitates were analyzed by SDS-PAGE and transferred to a nitrocellulose membrane (Cat#10600003, AmershamTM ProtranTM Premium 0.45 μm NC, GE Healthcare Life sciences). Monoclonal mouse anti-GFP antibodies were used (Cat#11814460001 ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein lysates were run on 10% polyacrylamide SDS-PAGE gels and transferred to nitrocellulose membranes (Amersham Protran 0.45 µm NC - GE Healthcare Life Sciences). Membranes were blocked for 30 minutes in 5% milk in TBST buffer and incubated overnight with primary antibody (1:1000) ...