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Citations for GE Life Sciences :
1701 - 1750 of 2545 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences, Marlborough, MA). After blocking in 5% non-fat dried milk powder (Biorad ...
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bioRxiv - Plant Biology 2021Quote: Total RNA (10 µg) was electrophoresed on a 1% formaldehyde denaturing gel and transferred onto the Hybond N+ membrane (GE Healthcare). The BrGypsy ...
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bioRxiv - Plant Biology 2021Quote: ... were separated onto a SDS-PAGE and proteins were transferred to Hybond-P PVDF transfer membrane (GE Healthcare, Amersham™ RPN303F) using the manufacturer’s instructions and a Trans-Blot TURBO Transfer System (Bio-Rad ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was then filter-sterilized using a 0.2 µm-pore size polyether sulfone (PES) membrane filter (Sterile Acrodisc, Gelman Sciences or Whatman, GE Life Sciences) to remove remaining bacterial cell debris ...
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bioRxiv - Biochemistry 2021Quote: ... Western blotting was performed with standard protocols using the PVDF membrane and signals visualized with ImageQuant LAS 4000 Luminescent Image Analyzer (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... The membrane was blocked for 1 h at room temperature in TBS containing 4% Amersham ECL Prime Blocking Reagent (GE Healthcare) and 0.1% Tween 20 ...
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bioRxiv - Cancer Biology 2021Quote: ... was resolved using 4–15% Mini-PROTEAN® TGX™ gels and transferred to Hybond-P 0.45µm PVDF membrane (GE Healthcare). Membranes were blocked in Roche block and incubated with primary antibodies over night or for 3 hours at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... and 10 ug were separated on an 0.8% agarose gel and transferred to a Nylon membrane (GE Healthcare Limited, Buckinghamshire, England) as described previously (36) ...
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bioRxiv - Microbiology 2020Quote: ... The reaction mixture containing the protein–DNA complex were resolved on a 5% native PAGE gel on ice and transferred to a nylon membrane (Amersham Hybond-XL, GE healthcare) using 0.5 X TBE ...
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bioRxiv - Cancer Biology 2020Quote: ... purified RNA was electrophoresed in non-denaturing 2% agarose TBE gel and then gel was blotted onto an Hybound-N nylon membrane (RPN303N, GE Healthcare) by semidry electroblotting in 0.5× TBE buffer for 2 hours at 200mA ...
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bioRxiv - Cell Biology 2021Quote: ... an equal amount of protein sample (between 10-20 μg) was separated by SDS-PAGE and then transferred onto a nitrocellulose membrane (Hybond, GE Healthcare) for two hours at 250 mA on ice ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants of the cell lysates were run on 12% resolving Tris-glycine gels and then transferred onto polyvinylidene difluoride (PVDF) membranes (GE Healthcare). To detect PstP-strep-tag II ...
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bioRxiv - Neuroscience 2022Quote: ... Co-immunoprecipitants were resolved on an 8% SDS-PAGE gel followed by protein transfer on nitrocellulose membrane (GE Healthcare Life Sciences). Western blot analysis was performed as described above.
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bioRxiv - Molecular Biology 2022Quote: Protein samples were run on 5∼20% precast gradient SDS-PAGE (ATTO) or 4∼20% precast gradient gel (Shanghai WSHT Biotechnology Inc.) and then transferred to Hybond ECL membranes (GE Healthcare) followed by western blot analysis with the indicated antibodies ...
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bioRxiv - Microbiology 2022Quote: ... An equal protein concentration or equal volume of cell and EV lysate was run in an SDS 10% polyacrylamide gel and subsequently transferred onto a nitrocellulose membrane (GE Healthcare). Total protein was measured by Ponceau S stain ...
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bioRxiv - Biophysics 2022Quote: ... The solution was passed through a 0.22 μm membrane filter and loaded onto a 5 ml HiTrap Q HP anion exchange column (GE Healthcare, USA) equilibrated with wash buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2022Quote: ... The proteins were separated by SDS–PAGE and transferred to a 0.45 μm nitrocellulose membrane (GE Healthcare Life Sciences, Marlborough, USA); the membranes were blocked with 1% nonfat milk powder and incubated together with primary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... put immediately on ice and 1 μL spotted on the positively charged nylon membrane (Amersham Hybon™-XL, GE Healthcare, France), which was exposed to UV at 254 nm for 10 min to fix DNA ...
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bioRxiv - Microbiology 2020Quote: ... Lysate equivalent of approximately 2×105 cells per well were run on NuPage 4-12% Bis-Tris Gel (Novex) and transferred to Amersham Hybond-P PVDF membranes (GE Healthcare). Membranes were blocked with 2% nonfat dry milk (Lab Scientific) ...
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bioRxiv - Microbiology 2021Quote: Cell lysates were separated on 10 % acrylamide gels by SDS-PAGE and protein transferred to a 0.45 μm PVDF membrane (GE Healthcare 15259894) using the BioRad TransBlot Turbo system ...
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bioRxiv - Microbiology 2020Quote: ... and 5 mM EDTA were pooled and concentrated by ultrafiltration on Amicon 10 kDa cut-of membrane and loaded on HiLoad 16/60 Superdex 75 pg (#28-9893-33, GE Healthcare) equilibrated with NaAc5.5 and fractions containing the proteolytic activity on azocasein were pooled and concentrated as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... normalized protein amounts were resolved under non-reducing conditions by SDS–PAGE and blotted to Hybond-P PVDF membrane (GE Healthcare) for 1 hour at 30 V ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were then incubated for 1 h with a secondary antibody (goat anti-rabbit horseradish peroxidase-linked IgG [NA934V GE Healthcare] or sheep anti-mouse horseradish peroxidase-linked IgG [NA931V GE Healthcare] ...
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bioRxiv - Neuroscience 2021Quote: ... was analyzed by electrophoresis on a denaturing 8% polyacrylamide gel containing 8 M urea followed by transfer to a positively-charge Hybond nylon membrane (GE Healthcare). Radioactive antisense RNA probes were in vitro transcribed from DNA oligonucleotide templates in the presence of [α32P]-UTP (3000 Ci/mmol ...
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bioRxiv - Microbiology 2021Quote: ... A constant amount of total RNA (between 3.5 and 14 μg) was loaded on 8% urea acrylamide gel in 1X TBE and transferred to a nitrocellulose membrane (GE Healthcare). For detection ...
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bioRxiv - Cancer Biology 2021Quote: ... protein extracts were resolved on SDS-PAGE gels under denaturing conditions and transferred to Hybond ECL membranes (Amersham Biosciences, GE Healthcare). Membranes were blocked for 1 hour at RT and incubated overnight with the respective primary antibody (Supplementary Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... a total of 50 μg of protein was separated on 7% sodium dodecyl sulfate polyacrylamide gel electrophoresis and then transferred onto nitrocellulose membrane (GE Healthcare) with a Novex Gel Transfert Device (ThermoScientific ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on a 12 % denaturing acrylamide gel and subsequently transferred onto a nitrocellulose membrane (Amersham Hybond-ECL, GE Healthcare). Membrane was blocked using 1x Roti®Block (Carl Roth ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were probed with primers 32P-labelled with [alpha-P32]Deoxycytidine 5’-triphosphate (dCTP) using Megaprime DNA labelling system (GE Healthcare) (for primers ...
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bioRxiv - Molecular Biology 2020Quote: Whole cell lysates were extracted from cells in 2x sample buffer and separated on 10% SDS– PAGE gel and transferred to nitrocellulose membranes (GE Healthcare). The membranes were blocked with Odyssey Blocking Buffer in PBS (Licor) ...
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bioRxiv - Plant Biology 2020Quote: ... Digested DNA was separated by electrophoresis on a 0.8 % agarose gel and then transferred onto Hybond N+ membrane (GE Healthcare, UK). Blots were hybridized with a digoxygenin (DIG)-labeled ZmPEPC promoter-specific probe synthesized using primers (Fwd 5’-TCCCGAGTTCCTAACCACAG ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies bound to the membrane were detected using horseradish peroxidase (HRP)-conjugated secondary antibodies against mouse or rabbit (GE Healthcare). Proteins were detected by chemiluminescence using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... 4-20% Tris glycine gel at 90V in MOPS-SDS buffer and transferred overnight at 30V to Amersham Hybond P 0.45 PVDF blotting membranes (GE Healthcare, 10600023). Membranes were blocked in 5% BSA for 2 hours at RT and incubated overnight with the following primary antibodies ...
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bioRxiv - Genetics 2021Quote: ... The membrane was washed 3 × 5 min in PBST and then visualized by Amersham ECL Prime Western Blot Detection Reagent (GE Healthcare). Images were taken by Fluorescent Image Analyzer LAS-3000 (FujiFilm ...
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bioRxiv - Plant Biology 2021Quote: ... and proteins were separated by SDS/PAGE on 8-10% (w/v polyacrylamide) gels and transferred to PVDF membranes (Semi-Dry-Blot, GE Healthcare). After transfer ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were then incubated with HRP-conjugated anti-mouse or anti-rabbit secondary antibodies (1:2500, GE Healthcare Life Sciences) diluted in 2% non-fat milk prepared in PBS for 1 hour at RT followed by 3 washes with TBS containing 0.1% Tween-20 for 5 minutes each at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... total protein (50 μg) was subjected to SDS-PAGE followed by transfer to polyvinylidene difluoride membrane (GE Healthcare Life Sciences, Europe) using a semidry trans-blotter (BioRad ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were washed three times 10 minutes and incubated for at least one hour in HRP-conjugated secondary antibodies (GE Healthcare) at 1:8000 dilution in PBS-T ...
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bioRxiv - Microbiology 2020Quote: ... 3 µg of RNA was loaded per well on a denaturing agarose gel and was blotted via capillary blot to Hybond(tm)-N+ membrane (GE Healthcare). The membranes were probed with [α32P]UTP-labeled transcripts generated using the MAXIscript® T7 In Vitro Transcription Kit (Ambion ...
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bioRxiv - Microbiology 2020Quote: ... Cell debris was pelleted at 15000 × g for 10 min and 10 μg of the cleared cell lysates were resolved on a 10% SDS-PAGE and transferred to 0.45 μm nitrocellulose membrane (GE life Sciences). Membranes were blocked in blocking buffer (5% non-fat milk ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was exposed to phosphor screens for several days and the radioactive signal was detected using Typhoon FLA7000 (GE Healthcare). ImageJ (NIH ...
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bioRxiv - Immunology 2020Quote: ... Samples were separated by protein electrophoresis at 120 V for 2 h using 10% Bis-Tris pre-cast gels (NuPage) and transferred overnight at 4°C onto Hybond-P PVDF membranes (GE Healthcare). After blocking in ECL blocking agent (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... boiled and separated by gel electrophoresis in Tris-glycine SDS (TGS) buffer (20 mM Tris, 192 mM glycine, 1 % (w/v) SDS) and transferred to a nitrocellulose membrane (GE Healthcare) in Tris glycine (TG ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Between 3-20 µg of total protein per sample were resolved by SDS-PAGE (4-20% gels) and transferred to Amersham Hybond P 0.45 PVDF blotting membranes (GE Healthcare, 10600023). Membranes were blocked overnight at 4 °C in 5% milk or 5% BSA in PBST ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by centrifugation at 35000 g for 30 minutes and the supernatant was passed through a 0.45 µ membrane filter before loading onto a pre-equilibrated HiTrapHP Strep column (GE Healthcare). The column was washed extensively with strep ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were blocked in non-fat 4% milk in TBS/T and incubated with primary and HRP-conjugated secondary (GE Healthcare) antibodies ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants from the cell lysate were run on 12% resolving Tris-Glycine gels and then transferred onto PVDF membrane (GE Healthcare). Rabbit α-strep antibody (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... or 90 V (secreted fraction and serum) at room temperature (RT) and electro-transferred to 0.45 µm nitrocellulose membranes (Amersham, GE Healthcare, Germany) for 1h30-2h at 0.5 A at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The membranes were washed three times with TBS-T and protein bands were visualized using the CDP-star detection reagent (GE Healthcare). Intensity was detected and quantitatively analyzed by the Fluor-S MAX multi-imager system (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μL of each homogenate was loaded onto the nitrocellulose membrane (Amersham Protran 0.45; GE Healthcare Life Sciences, Chicago, IL, USA) and left to dry out at room temperature ...