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Citations for GE Life Sciences :
1801 - 1850 of 2545 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... the gels were run in 1× RNA running buffer (1 × MOPS and 6% [v/v] formaldehyde) and blotted onto the Hybond-N+ membrane (GE Healthcare) soaked in 20× SSC (3.0 M NaCl and 0.3 sodium citrate) ...
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bioRxiv - Genomics 2022Quote: ... Samples were then loaded onto a 1% agarose gel containing 3% formaldehyde and 1x MOPS and run at 75 V in 1x MOPS for ~3 hs after which RNA was transferred onto a Hybond N+ membrane (GE Healthcare) overnight ...
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bioRxiv - Microbiology 2022Quote: ... Proteins (40 µg) were run on 10% SDS–PAGE gel and transferred to an Amersham Hybond-ECL nitrocellulose membrane (GE healthcare), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 or 10 μg of the purified RNA was resolved on formaldehyde agarose gels and transferred onto nylon membranes (GE Healthcare) by capillary blotting and fixed by UV-crosslinking ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed twice in PBS-T before being visualized on a Typhoon Trio Scanner (GE Healthcare, Piscataway, NJ, USA) and quantified with ImageJ (Abramoff et al. ...
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bioRxiv - Plant Biology 2022Quote: ... equal amounts of proteins were separated on a 10% or 12% SDS-polyacrylamide (PA) gel and transferred to nitrocellulose membranes (GE Healthcare). The signal was probed with specific antibodies using Clarity Western ECL Blotting Substrate (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... Samples were resolved on 12–15% SDS–PAGE gels and then proteins were wet transferred to Protran nitrocellulose membranes (GE Healthcare) at 400 mA at 4 °C for 1.5 h in 1x Transfer buffer (25 mM Tris base ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were resolved by electrophoresis on 10 or 12% SDS-PAGE gels and transferred onto polyvinylidene fluoride (PVDF) membranes (GE Healthcare). Total protein labeling was performed using No Stain Labeling Reagent (Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... TCA extracts of total yeast or 2 μg of mitochondrial proteins were loaded on a 12 % SDS-PAGE and transferred onto a nitrocellulose membrane (GE Healthcare). UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD) ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... Sonicated mixture was run through serial centrifugation units with Ultracel regenerated cellulose membrane in a range of molecular weight cutoffs (Vivaspin or GE Healthcare) from 100k to 3k Daltons ...
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bioRxiv - Plant Biology 2023Quote: Equal amounts of protein extracts were electrophoresed on 12% sodium dodecyl sulphate (SDS) polyacrylamide gel prior to transfer on Hybond ECL nitrocellulose membranes (GE Healthcare). Homogenous transfer was confirmed by Ponceau Red staining ...
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bioRxiv - Cancer Biology 2023Quote: ... separated by denaturing 12.5 % SDS-polyacrylamide gel and transferred to Amersham™ Hybond™ 0.45 μm PVDF membrane (GE Healthcare, UK). The membrane was blocked for 1 h with a blocking buffer (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... Cell lysates and samples were separated by standard SDS–PAGE or Tricine-SDS-PAGE (for HCMV US18/US20 blots) and transferred to PVDF membrane (GE Healthcare) using wet transfer in Towbin blotting buffer (25 mM Tris ...
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bioRxiv - Genetics 2023Quote: 10 µg RNAs were separated on a 1.2% agarose gel and transferred overnight by capillarity on a nylon membrane (GE Healthcare, #RPN203B). RNAs were then UV-crosslinked to the membrane using a Stratalinker apparatus ...
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bioRxiv - Cell Biology 2023Quote: ... by capillarity and membranes were hybridized with a 32P-labelled oligonucleotide probe (23S.1, 5’- GATTGCTCGAATGCCCAAAG-3’) using Rapid-hyb buffer (GE Healthcare). Radioactive membranes were exposed to Phosphorimaging screens and analysed using a Typhoon Tryo (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... Reduced immunoprecipitated proteins were separated on a 10 or 12% SDS-polyacrylamide gel and transferred to a PVDF membrane (GE Healthcare). Details on the blocking of the membranes and detection of the proteins can be found in Table S1 ...
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bioRxiv - Immunology 2022Quote: ... Proteins in gels were either stained with Coomassie blue dye in gel or used for transfer onto nitrocellulose membranes (GE Healthcare). The membranes were blocked with 5% milk in PBST (PBS and 0.05% Tween-20 ...
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bioRxiv - Biochemistry 2023Quote: ... without heating) were loaded and subjected to electrophoresis on 15% SDS-polyacrylamide gels and transferred to a nitrocellulose membrane (GE Healthcare) in transfer buffer (25 mM Tris ...
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bioRxiv - Plant Biology 2023Quote: ... and 5 μg of total RNA was separated by 15% (wt/vol) acrylamide/7 M urea gel and subsequently transferred to a Hybond-NX nylon membrane (GE healthcare) after gel electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... the membrane was exposed for several hours to a phosphoimaging screen (Kodak) and visualized using a Typhoon FLA 9500 (GE Healthcare), followed by western blot incubation.
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bioRxiv - Genetics 2024Quote: ... Whereafter the proteins were separated by SDS-PAGE and transferred onto nitrocellulose blotting membranes with a pore size of 0.45 μm (GE Healthcare, 10600002). The membranes were blocked with TBS-Tween buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... or PIG-B (53,54) and fractionated on 10-12% polyacrylamide gel electrophoresis (PAGE)-urea gel and transferred to Amersham Hybond-N+ membrane (GE Healthcare). Transferred membranes were crosslinked 2x with a UV crosslinker (UVP ...
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bioRxiv - Microbiology 2024Quote: ... were electrophoresed on 10% sodium dodecyl sulphate-polyacrylamide gel and the separated proteins were transferred onto the PVDF membranes (Amersham International, GE Healthcare). The membranes were blocked with Tris-buffered saline with Tween (TBST ...
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bioRxiv - Microbiology 2024Quote: ... Protein samples were resolved by stain-free sodium dodecyl sulfate–10% polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a nitrocellulose membrane (GE Healthcare) using the semi-dry Trans-Blot turbo transfer system (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated by standard SDS-PAGE and transferred to an Immobilon®-P PVDF Membrane (GE Healthcare, Chicago, IL, USA). Blots were blocked overnight at 4°C in blocking buffer (PBS + 0.05 % Tween 20 + 2% Amersham ECL Prime Blocking Reagent ...
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bioRxiv - Microbiology 2024Quote: ... the DNA molecules that were resolved on the gel were transferred on to the nylon membrane (Amersham Hybond N+, GE Healthcare) by capillary action ...
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bioRxiv - Cancer Biology 2024Quote: ... The denatured proteins were resolved on 8–12 polyacrylamide-SDS gels and transferred onto PVDF membrane (GE Healthcare, Chicago, IL, USA). A molecular weight marker was added onto each gel for reference (Precision Plus Protein Dual Color ...
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bioRxiv - Neuroscience 2024Quote: ... Protein was separated on an 8-16% gradient Tris-glycine gels and transferred to PVDF blotting membrane (GE Healthcare Life Sciences). The membrane was immunoblotted with 1:200 anti-FGF13 antibody or 1:200 anti-pan FHF-A (Neuromab clone N235/22 ...
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bioRxiv - Molecular Biology 2024Quote: An appropriate amount (0.5 – 2 µg) of total RNA was separated in 1% MOPS-formaldehyde agarose gels and transferred to Hybond-N + membranes (GE Healthcare). Membranes were exposed to either randomly [32P]-labelled dsDNA probes ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 µg of isolated genomic DNA were digested with SacII and resulting fragments were separated by size on an 0.8 % agarose gel and transferred to a nylon membrane Hybond™-N+ (GE Healthcare) via capillary blot ...
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bioRxiv - Cell Biology 2024Quote: Proteins were separated using SDS-PAGE in 10% Bis-Tris acrylamide/ bisacrylamide gels and transferred to a nitrocellulose membrane (GE Healthcare). The membranes were blocked for 30 min with PBS 5% milk and incubated with the first antibody at 4 °C overnight with gentle shaking ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of total cell-lysates were resolved in Tris-Glycine SDS-PAGE and transferred on to a Protran nitrocellulose membrane (Cat#GE10600002, GE Healthcare). The efficacy of protein transfer to membranes was confirmed by staining with 1% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was filtered through a 0.45 µm membrane and loaded onto a nickel affinity column (5 mL GE Healthcare HisTrap). The protein was eluted using a gradient from 20 to 500 mM imidazole (solution B ...
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bioRxiv - Cell Biology 2024Quote: ... Between 5-20 μg of total protein per sample were resolved by SDS-PAGE (4-20% gels) and transferred to Amersham Hybond P 0.45 PVDF blotting membranes (GE Healthcare, 10600023). Membranes were blocked overnight at 4°C in 5% skim milk powder or 5% BSA in PBS 0.1% Tween when working with phospho-specific antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Whole cell lysates were fractionated using 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and electrotransferred onto polyvinylidene difluoride membranes (GE Healthcare). Western blotting was performed according to standard protocols [24].
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bioRxiv - Developmental Biology 2024Quote: ... Proteins (20 μg/lane) were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (10600023, GE Healthcare). After transfer ...
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bioRxiv - Developmental Biology 2024Quote: ... Normalized protein samples were resolved on 4-20% tris-glycine gels and transferred to Amersham Hybond P 0.45 PVDF blotting membranes (GE Healthcare, 10600023). Membranes were blocked for 1h at room temperature in PBS-T with 5% milk ...
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bioRxiv - Cell Biology 2020Quote: ... western blot based GST pull-down assay was performed in which Glutathione Sepharose® 4B beads (GE Healthcare) was coupled with 10 µg of purified r14-3-3I protein in buffer A (20 mM Tris ...
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bioRxiv - Immunology 2021Quote: Surface plasmon resonance assays were performed by a BIAcore T100 instrument with a CM5 sensor chip (GE Healthcare). Experiments were carried out at 25°C in binding buffer (PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Flag primary antibody (monoclonal M2 mouse IgG1) and enhanced chemiluminescence assay (Amersham ECL Primer, GE Healthcare Life Science) were used for detection.
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bioRxiv - Molecular Biology 2021Quote: ... Yeast cells at an OD600 of approximately 0.8 were rapidly harvested by vacuum filtration through a 0.45 μm Whatman cellulose nitrate membrane filter (GE Healthcare Life Sciences, USA) at room temperature by scraping the slurry into liquid nitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... The Nitrocellulose membrane was rinsed in phosphate buffered saline and placed in a phosphorimage cassette and a film (GE Healthcare, Chicago, IL) exposed for 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... or 8 % denaturing polyacrylamide gel (PAGE) and then transferred to a nylon membrane (Biodyne® A, PALL or Hybond-N+, GE Healthcare). After transfer ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were exposed to a photosensitive Phosphor screen and visualized with an Amersham Typhoon IP Biomolecular Imager (GE Healthcare Life Sciences).
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bioRxiv - Molecular Biology 2021Quote: ... Gels were either stained with Coomassie Brilliant Blue (CBB) for justifying equal loading or transferred onto PVDF membranes (Amersham, GE Healthcare Lifescience) proceeding further for western blotting ...
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bioRxiv - Genomics 2021Quote: ... Neisseria sicca and Neisseria elongata was migrated on a 6% polyacrylamide gel and then transferred onto nitrocellulose membrane (Amersham Hybond™ N+ from GE healthcare). The oligonucleotides were labeled in 5’ by using 5 pmoles of oligonucleotide ...
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bioRxiv - Genetics 2020Quote: Incorporation of [α-32P]-dCTP was measured by spotting 5 μl aliquots of the reaction mixture (after the indicated time points at 37°C) on Hybond N+ membrane strips (GE Healthcare Lifesciences). The membranes were washed (3 × with 2 × SSC and 1 × with 95% EtOH ...
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bioRxiv - Microbiology 2021Quote: Whole cell protein lysate from 1 × 106 trypanosome cells was separated on 12.5% sodium dodecyl sulphate (SDS)-polyacrylamide gels and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences). The membranes were blocked by incubation with 5% milk powder in PBS for 1 h at room temperature (RT ...
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bioRxiv - Biochemistry 2020Quote: Samples contain 50 μg total protein were loaded on a 10 % or 16.5 % acrylamide gel for SDS-PAGE and then transferred to a nitrocellulose membrane (GE Life Sciences #10600002). Membranes were incubated in blocking buffer (Thermo Scientific #37539 ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were separated by SDS-gel electrophoresis (10% Acrylamide) and transferred to nitrocellulose membrane (Amersham™ Protran™ 0,45μm NC, GE Healthcare Lifescience). Quality of transfer was confirmed by staining with Ponceau-S solution (Sigma Aldrich) ...