Labshake search
Citations for Quantifoil Micro Tools :
101 - 150 of 1446 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: Cells were transiently transfected with FKBP8N403K-mScarlet plasmid for 24 hours and then were transferred to cryo-EM grids (Quantifoil, 200-Au-mesh with carbon film) (pre-treated with 50 ug/ml fibronectin for at least 15 minutes ...
-
bioRxiv - Cell Biology 2024Quote: HeLa WT cells or HeLa cells stably expressing Apaf1-GFP were plated on 200 mesh gold EM grids with a holey carbon film R2/2 (Quantifoil). HeLa WT cells were transfected with Apaf1-SNAP-tag ...
-
bioRxiv - Cell Biology 2024Quote: ... Glow-discharged Cryo-EM grids (R1.2/1.3, Cu 200 mesh grid, Quantifoil microtools) were mounted on a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2024Quote: Cells were seeded and grown overnight on Quantifoil carbon film gold grids (Quantifoil R 1/4 on 200 gold mesh) coated with 10 µg/ml FN ...
-
bioRxiv - Cell Biology 2024Quote: ... Cu supported 3.5/1 Holey Carbon grids (Quantifoil) were glow discharged on both sides for six seconds each using atmospheric air to render them more hydrophilic ...
-
bioRxiv - Microbiology 2024Quote: ... 3 µL aliquots were applied to glow-discharged holey carbon grids (Quantifoil R1.2/1.3 300 mesh, Ted Pella) and incubated for 10 s before automated blotting and flash-freezing in liquid ethane with a Vitrobot Mark IV vitrification system (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2024Quote: ... UltrAuFoil holey grids (R1.2/1.3, Quantifoil, Großlöbichau, Germany). Blotting was performed using a Vitrobot Mark IV ...
-
bioRxiv - Molecular Biology 2024Quote: ... 300 mesh copper grids (Quantifoil) were glow discharged using a Tergeo Plasma Cleaner (Pie Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... 3 µL of the PURE system reaction solution containing stalled ribosomes was applied onto a glow-discharged holey-carbon grid coated with continuous 2 nm-thick carbon film (Quantifoil Au 300 mesh, R2/1 + 2 nm C) and incubated for 30 s in a controlled environment of 100% humidity at 4 ℃ ...
-
bioRxiv - Biophysics 2024Quote: R1.2/1.3 300 mesh copper grids (Quantifoil; Electron Microscopy Sciences) were glow discharged for 90 seconds at 20 mA before cryo-sample freezing process ...
-
bioRxiv - Biophysics 2024Quote: Copper girds (Quantifoil, 1.2 μm diameter holes ...
-
bioRxiv - Biophysics 2024Quote: ... The cryo-EM grids were prepared by applying 3.5 μl protein (3.5 mg/mL) to a glow-discharged Quantifoil R1.2/1.3 200-mesh copper holey carbon grid (Quantifoil, Micro Tools GmbH) and blotted for 3.0 s under 100% humidity at 4 °C before being plunged into liquid ethane using a Mark IV Vitrobot (FEI) ...
-
bioRxiv - Biophysics 2024Quote: 3 μL of concentrated protein sample was applied to a glow-discharged (30 s at 15 mA) grid (Quantifoil Au or Cu R1.2/1.3 300 mesh) and after a wait time of 5 s ...
-
bioRxiv - Biophysics 2024Quote: ... Vero cells were seeded either on gold finder grids (200 mesh, R2/2, Quantifoil, Jena, Germany) or gold grids coated with lacey carbon film (XXBR Technology ...
-
bioRxiv - Microbiology 2024Quote: ... 3.5 μL of the purified complex at 0.8 mg/mL was applied to glow-discharged Cu R1.2/1.3 holey carbon grid (200 mesh, Quantifoil) with a final concentration of 1% glycerol ...
-
bioRxiv - Biochemistry 2024Quote: ... 3 µL of proteins / proteins-phospholipids mix samples were gently deposited on glow discharged Quantifoil R 1.2/1.3 holey carbon grids (Quantifoil Micro Tools GmbH, Germany). Samples were manually blotted for four seconds at room temperature and vitrified by rapidly plunging into liquid ethane using a home-built plunging apparatus ...
-
bioRxiv - Cell Biology 2024Quote: ... EM grids (Quantifoil R2/1 Au 300 ...
-
bioRxiv - Molecular Biology 2024Quote: ... carbon R2/1 foil grids (Quantifoil Micro Tools GmbH, Germany) were pre-clipped into an autogrid ring (Thermo Fisher Scientific Inc ...
-
bioRxiv - Biophysics 2024Quote: ... UltrAuFoil R1.2/1.3 300-mesh gold grids (Quantifoil) were glow-discharged at 25 mA for 80 s ...
-
bioRxiv - Biophysics 2024Quote: ... samples that have been analyzed here are Influenza A/Hong Kong/1/1968 virions that are produced from MDCK cells grown directly on cryoEM grids (QF AU200 R 2/2, Quantifoil, Germany), infected at a multiplicity of infection <1 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Grids were prepared by applying 3.5 μLs of the Z7757 complex or 2 μLs of the Lisuride complex to glow discharged UltrAuFoil holey gold grids (Quantifoil, Au300-R1.2/1.3). The girds containing Z7757 were then vitrified by plunge freezing into a 60/40 mixure of liquid ethane/propane using a Vitrobot mark IV (FEI ...
-
bioRxiv - Cell Biology 2024Quote: ... Each glow-discharged Quantifoil R2/4 200 mesh copper grid (Quantifoil Micro Tools GmbH, Jena, Germany) was then deposited with 4 µL sample and plunge-frozen in liquid ethane using a Vitrobot Mark IV (TFS ...
-
bioRxiv - Bioengineering 2024Quote: ... LCNP or EV suspensions in PBS (∼1 × 1012 particles/mL) were deposited on 300-mesh lacey carbon-coated copper grids (Quantifoil Micro Tools GmbH, Germany). The grids were previously glow-discharged for 45 s using an Emitech K100X glow discharge system (Quorum Technologies Ltd. ...
-
bioRxiv - Biophysics 2024Quote: ... We glow-discharge-treated 200 mesh R1/4 or R2/4 Quantifoil holey carbon gold grids (Quantifoil®, Grossloebichau/Jena Germany) in a Pelco easiGlow (Ted Pella ...
-
bioRxiv - Biophysics 2024Quote: ... R1.2/1.3 or R2/2 UltrAuFoil® grids (Quantifoil Micro Tools GmbH) were glow-discharged with 40 mA for 5 min inside a Quorum GloQube® Plus unit ...
-
bioRxiv - Biophysics 2024Quote: ... Three µl of the sample was applied to Quantifoil 300 mesh R1.2/1.3 grids (Quantifoil, EMS) that were glow discharged using a Pelco EasiGlo ...
-
bioRxiv - Biophysics 2024Quote: ... gold grids coated with holey carbon film (300 mesh, R2/2, Quantifoil, Jena, Germany) were glow-discharged and UV-treated for 1 h ...
-
bioRxiv - Biophysics 2024Quote: ... a total of 2.5 μl of the nanodisc-reconstituted pdP2X7 was applied to a glow-discharged holey carbon-film grid (Quantifoil, Au 1.2/1.3, 300 mesh, USA). The grid was then blotted using a Vitrobot (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... The sEV samples were frozen on TEM grids (Quantifoil R2,2 300 mesh, EMS) that were charged for 30 seconds with a Pelco EasiGlow glow discharge cleaning system (Ted Pella) ...
-
bioRxiv - Biochemistry 2024Quote: ... Au 300 mesh (Quantifoil), then freezing using a manual plunger in the cold room at 4°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... Four microliters of the sample were applied onto 300 mesh R1.2/1.3 UltrAuFoil Holey Gold Films (Quantifoil) that were glow discharged for 25 seconds at 15 mA with a Pelco Easiglow 91000 (Ted Pella ...
-
bioRxiv - Molecular Biology 2024Quote: ... 300 mesh holy carbon grids (Quantifoil, Jena, Germany) which had been glow discharged with 15 mA at 0.39 mBar for 1 min ...
-
bioRxiv - Biochemistry 2024Quote: ... A4>p or A4p in the reaction buffer Y supplemented with 1 mM DTT and 2.5% glycerol just before applying on the freshly glow-discharged copper 300 mesh R1.2/1.3 holey carbon grids (Quantifoil), in a Vitrobot Mark IV (FEI ...
-
bioRxiv - Biophysics 2024Quote: ... Regions of the LCP drops were scooped and gently smeared across pre-clipped Cu200 R2:2 grids (Quantifoil) under a humidifier ...
-
bioRxiv - Biophysics 2024Quote: ... 4 × 104 MDM were seeded on glow discharged and UV-light sterilized 200-mesh EM gold grids coated with R 2/2 holey carbon films (Quantifoil Micro Tool GmbH), which were placed in a glass-bottomed ‘microwell’ of 35-mm MatTek dish (MatTek ...
-
bioRxiv - Bioengineering 2024Quote: ... The Vitrobot Mark IV system (FEI) was used to plunge-freeze a copper lacey carbon film-coated grid (Quantifoil, R1.2/1.3 300 Cu mesh). U155@lipids (10 µL ...
-
bioRxiv - Biochemistry 2024Quote: ... Quantifoil R1.2/1.3 Cu 300 grids coated with a holy carbon film (Quantifoil) were treated for hydrophilisation using a JEC-3000FC Auto Fine Coater (JEOL ...
-
bioRxiv - Biophysics 2024Quote: ... was applied to glow-discharged gold grids coated with either holey carbon film (Quantifoil, 300 mesh 1.2/1.3, Au) or holey gold film (UltrAuFoil ...
-
bioRxiv - Neuroscience 2024Quote: ... Plates were shaken at 1,200 rpm for 5 mins at 30°C using a plate shaker (Quantifoil Instruments) and absorption of 405 nm light measured using a FlexStation (Molecular Devices).
-
bioRxiv - Molecular Biology 2024Quote: 3 μL aliquot of the β2AR-Gi-scfv16 complex was applied onto glow-discharged 300 mesh grids (Ultraufoil R1.2/1.3 or Quantifoil R1.2/1.3) and vitrified using a Vitrobot Mark IV (Thermo Fischer Scientific ...
-
bioRxiv - Biochemistry 2024Quote: 5 mg/mL ROCKET and ROCKETAAXWA with 100 µM CDL were frozen on Quantifoil 1.2/1.3 Au 300 mesh grids (Quantifoil Micro Tools). 3 µL of sample was applied to each grid which was then blotted for 3 s and plunge-frozen into liquid ethane using FEI Virtobot Mark IV (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2024Quote: ... to a glow-discharged Quantifoil R1.2/1.3 200/300-mesh copper/gold holey carbon grid (QUANTIFOIL, Micro Tools GmbH, Germany) and blotted for 3.0 s under conditions of 100% relative humidity and 4°C before being plunged into liquid ethane using a Mark IV Vitrobot (FEI) ...
-
bioRxiv - Biochemistry 2024Quote: ... Au 300 mesh (Quantifoil). The Cryo-EM grids were prepared for SgrAIK242A/40-1/Mg2+ filaments using the same procedures as those used for preparing grids for SgrAIWT/40-1/Mg2+ filaments ...
-
bioRxiv - Biochemistry 2024Quote: ... Quantifoil R1.2/1.3 400-square mesh Cu grids (Quantifoil) were glow-discharged for 1 minute at 10 mA using a PELCO easiGLow glow discharging system (Ted Pella ...
-
bioRxiv - Biochemistry 2024Quote: ... Grids (Quantifoil Cu R1.2/1.3, 400 mesh) were glow-discharged for 30 s and 3.5 µL of the protein at 1.0 to 3.0 mg/ml were applied to the grids and blotted for 3.5 s before being flash-frozen in liquid ethane cooled by liquid nitrogen with Vitrobot Mark IV (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... The sample solution (3 μl) was applied to glow-discharged (20 mAmp, 30 sec, Quorum GloQube) Quantifoil R1.2/1.3 grids (Quantifoil Micro Tools GmbH), blotted for 5 s using blot force 0 and plunge frozen into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2024Quote: ... A 4.5 µL droplet of the culture was applied to glow discharged copper grids (Quantifoil R2/2, Quantifoil Micro Tools) and blotted for 2.5 s in a 95% humidity chamber at 20 °C ...
-
bioRxiv - Biophysics 2024Quote: ... 3 μl sample were applied individually to electron microscopy grids (Quantifoil, 400 mesh Au 1.2/1.3) that were glow discharged for 40 s using Gatan SOLARUS ...
-
bioRxiv - Biophysics 2024Quote: ... 2 μL of 2 μM taxol-microtubules were incubated on a glow-discharged holey carbon cryo-EM grid (QuantiFoil, Cu 300 R 2/1) for 30 seconds ...
-
bioRxiv - Biophysics 2024Quote: ... 2 μL of 2 μM taxol-microtubules were incubated on a glow-discharged holey carbon cryo-EM grid (QuantiFoil, Au 300 R 1.2/1.3) for 30 seconds ...