Labshake search
Citations for Quantifoil Micro Tools :
401 - 450 of 1446 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... respectively) and 3 μl were kept 5 min at RT until their application onto glow-discharged holey carbon grids (Quantifoil, Au 300 mesh, R 0.6/1). The grids were blotted and then plunged into liquid ethane using a FEI Vitrobot Mark IV at 20°C and 95% relative humidity ...
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bioRxiv - Bioengineering 2023Quote: ... a 200-mesh copper grid covered with a Quantifoil R 2/2 holey carbon film (Quantifoil Micro Tools GmbH) was surface plasma treated for 40 seconds using a Cressington 208 carbon coater ...
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bioRxiv - Biophysics 2023Quote: ... an aliquot of 2.2 μl sample was applied to the plasma-cleaned holey carbon grid (Quantifoil R1.2/1.3, Cu, 400 mesh). The grid was blotted using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... 3 μl of the sample was applied to a hydrophilized grids (Quantifoil, R1.2/1.3+2nm carbon) with an additional 2 nm layer of amorphous carbon ...
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bioRxiv - Biophysics 2023Quote: ... 3.5 μL of labeled GltPh protein (4.5 mg/mL) was applied to a glow-discharged QuantiFoil R1.2/1.3 300-mesh gold grid (Quantifoil Micro Tools GmbH) and incubated for 2 min under 100 % humidity at the desired temperatures ...
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bioRxiv - Biophysics 2023Quote: ... 4 μL of Kapβ2•HNRNPH2 was applied to a 300 mesh copper grid (Quantifoil R1.2/1.3) that was glow-discharged using a PELCO easiGlow glow discharge apparatus at 30 mA/30 s on top of a metal grid holder (Ted Pella) ...
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bioRxiv - Biophysics 2023Quote: Purified TANAV particles were loaded onto a R1.2/1.3 Mo Quantifoil holey carbon grid (Quantifoil Micro Tools) and flash-frozen in liquid ethane using a Vitorobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: Carbon-coated gold TEM grids (Quantifoil NH2A R2/2) were glow-discharged at 2 mA and 1.5– × 10−1 m bar for 1 minute in an ELMO (Cordouan ...
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bioRxiv - Biophysics 2023Quote: ... Micropatterns covered an area of 8 × 8 squares around the center of the cryo-TEM grids (Quantifoil R1/4 or R1/20, Au 200 mesh, SiO2 film) as previously described31 ...
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bioRxiv - Biophysics 2023Quote: ... Aliquots of 4 μl of protein samples (0.3 mg ml-1 or ∼6 μM) were applied to glow-discharged R1.2/1.3 200 Cu mesh grids (Quantifoil). After 30 s ...
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bioRxiv - Biophysics 2023Quote: ... 4 μl of the extracellular parasites were pipetted onto a glow-discharged (30 seconds at −30 mA) copper R2/2 holey carbon grid (Quantifoil Micro Tools GmbH, Jena, Germany). Samples were back-blotted with a Whatman filter paper (grade 1 ...
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bioRxiv - Biophysics 2023Quote: ... split onto Poly-D-Lysine coated R 2/2 grids (QUANTIFOIL 100 Holey Carbon Films ...
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bioRxiv - Biophysics 2023Quote: ... 300-mesh Cu R1.2/1.3 grids (Quantifoil Micro Tools, Germany) were glow-discharged under H2-O2 condition for 60 s ...
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bioRxiv - Biophysics 2023Quote: ... the sample was applied to holey carbon-film grids (Quantifoil, Germany ...
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bioRxiv - Biophysics 2023Quote: The freshly purified CD69-S1PR1-Gi-scFv16 complex was added to Quantifoil R1.2/1.3 400-mesh Au holey carbon grids (Quantifoil), blotted using a Vitrobot Mark IV (FEI) ...
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bioRxiv - Molecular Biology 2023Quote: 1.8 × 105 A549 or A549-IFITM3 cells were seeded on glow-discharged 200 mesh gold grids with a holey SiO2 film with R1/4 spacing (Quantifoil) placed in a 35 mm dish and cultured in 2 ml complete growth medium (DMEM-F12 ...
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bioRxiv - Biochemistry 2023Quote: ... To each 200-mesh holey carbon grid (Au R1.2/1.3, Quantifoil Micro Tools), 2.5 µl of sample was applied and blotted for 4 s at 80% humidity and 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were applied to freshly glow-discharged (Edwards S150B, 45 s, 30−50 mA, 1.2 kV, 10−2 mbar) copper holey carbon grids (Quantifoil, Cu-R1.2/1.3) under 100% humidity at 4°C.
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bioRxiv - Biochemistry 2023Quote: ... and 260 μL of chloroform (CHCl3) were added to each tube and vortexed thoroughly before incubating in a Bioshake iQ (Quantifoil Instrument GmbH, Germany) setting at 1,400 rpm for 30 minutes and centrifuged at 14,000 rpm for 15 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Quantifoil R 1.2/1.3 200 mesh Copper TEM grids (Quantifoil Microtools) and blotted for 3 sec using a Mark IV Vitrobot (FEI ...
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bioRxiv - Biochemistry 2023Quote: 4 µL of protein solution (total protein concentration = 1 mg/mL) were applied to freshly glow-discharged QUANTIFOIL® R2/1 300 mesh grids (Quantifoil Micro Tools GmbH ...
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bioRxiv - Biochemistry 2023Quote: ... An aliquot (2 μL) of this polarized vesicle solution was applied onto a glow-discharged holey gold grid (Quantifoil Au R2/2, 400 mesh). After incubating the sample on the grid for 3 minutes at 10 °C with a humidity of 100% ...
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bioRxiv - Biochemistry 2023Quote: 4.5 µL of freshly mixed Nup98 condensate and capsid mixture with protein A-gold (10 nm) were applied onto a glow-discharged Quantifoil R2/2 copper grid (Quantifoil Micro Tools). The grid was blotted in the front for 2.5 s at 15 °C with 90% relative humidity and then plunged into liquid ethane using a Lecia EM GP device (Lecia Microsystem) ...
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bioRxiv - Biochemistry 2023Quote: ... R1.2/1.3 holey carbon grids (Quantifoil) were cleaned using a plasma cleaner for 30 sec on medium strength ...
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bioRxiv - Biochemistry 2023Quote: ... 3µL of sample were frozen in liquid ethane on 2/1 Quantifoil Multi A holey carbon supported grids (Quantifoil, Multi A, 400 mesh) using Vitrobot Mark IV (FEI Company) ...
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bioRxiv - Biochemistry 2023Quote: 3.5 µl purified TOL-1–LAT-1 full-ectodomain complex (0.28 mg/ml) was applied on glow-discharged holey carbon grids (Quantifoil R1.2/1.3, 200 mesh), and vitrified using a Vitrobot Mark IV (FEI Company) ...
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bioRxiv - Biochemistry 2023Quote: Purified ternary complex was vitrified on holey-carbon grids (Quantifoil R 2/1) using FEI Vitrobot Mark IV ...
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bioRxiv - Molecular Biology 2023Quote: Four μL of 0.5 mg/ml CRY:TIM solution was applied to glow-discharged grids (300 mesh Quantifoil Au, R1.2/1.3) that were purchased from Electron Microscopy Sciences ...
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bioRxiv - Immunology 2023Quote: ... Then 3 μl of the mixture was applied to a Quantifoil-Au-2/1-3C grid (Quantifoil) pretreated by glow-discharging at 20 mA for 1 min ...
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bioRxiv - Biochemistry 2023Quote: ... Holey carbon copper grids (Quantifoil R1.2/1.3) were glow discharged (GloQube Plus ...
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bioRxiv - Microbiology 2023Quote: ... 2.2µl of the purified mixture at a concentration of 0.5 mg/mL was applied to glow discharged (30 s at 25mA) grid (300 mesh 1.2/1.3, Quantifoil). The grids were blotted for 3.5 s before plunging into liquid ethane using Vitrobot MK4 (TFS ...
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bioRxiv - Biophysics 2023Quote: ... 3.5 μl of the sample was applied to a freshly glow-discharged (PELCO easiGlow, 30 mA for 120s) 1.2/1.3 UltrAuFoil grid (Quantifoil), which were blotted for 2.5s and plunge-frozen using a Leica EM GP2 plunger at 14 °C and 99% humidity ...
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Mechanism of RanGTP priming H2A-H2B release from Kap114 in an atypical RanGTP•Kap114•H2A-H2B complexbioRxiv - Biochemistry 2023Quote: ... 4 μL of Kap114•H2A-H2B/RanGTP or 3.5 μL of Imp9•RanGTP was applied to a 300 mesh copper grid (Quantifoil R1.2/1.3) that was glow-discharged using a PELCO easiGlow glow discharge apparatus for 30 mA/30 s on top of a metal grid holder (Ted Pella) ...
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bioRxiv - Biochemistry 2023Quote: 3 μl of 50 μM purified insulin in 20 mM sodium phosphate buffer containing (pH 7.5) containing 100 mM NaCl was applied onto a grow discharged Quantifoil R1.2/1.3 Cu 200 grid (Quantifoil Micro Tool GmbH) and subsequently blotted by filter papers and plunged into liquid ethane for the vitrification using a Vitrobot Mark IV (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... by applying protein to glow-discharged QuantiFoil Au R1.2/1.3 200-mesh grids (Quantifoil), blotting for 3.0 s under 100% humidity at 4°C ...
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bioRxiv - Biophysics 2023Quote: 3.5 µL of protein or lipid solution was applied onto a freshly glow-discharged carbon-coated copper mesh grid (Quantifoil). After 1 min ...
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bioRxiv - Biophysics 2023Quote: ... and 10 nm gold fiducials (1:5 (v/v)) was applied to glow-discharged Quantifoil grids (QF-1.2/1.3, 200-mesh holey carbon on copper, Quantifoil) on a Leica GP2 vitrification robot (Leica ...
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bioRxiv - Biophysics 2023Quote: ... All cryo-EMsamples mixed with 0.006 % Tween-20 were applied onto a glow-discharged R1.2/1.3 cooper or gold 300-mesh holey carbon grid (Quantifoil) and were immediately frozen in a liquid ethane-propane mixture using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: MEFs were grown to ∼80% confluency on glow-discharged holey carbon EM grids (R2/1, Au 200 mesh; Quantifoil, Jena, Germany) prior to preparation for cryo-ET analysis ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µl of the VIF-ΔT solution was applied onto glow-discharged holey carbon EM grids (Cu R2/1, 200 mesh, Quantifoil), which were subsequently blotted manually and plunge frozen in liquid ethane.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... a volume of 6-8 μL of each sample was applied onto a gold grid covered by a holey gold film (UltrAuFoil 2/1, Quantifoil Micro Tools GmbH, Jena, Germany). Excess of liquid was blotted automatically between two strips of filter paper or only from the backside of the Grid ...
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bioRxiv - Plant Biology 2023Quote: Three µl of purified PSI at a concentration of 3 mg/ml was applied to the glow-discharged holey carbon grids (Cu QUANTIFOIL® R1.2/1.3), then vitrified using a Leica GP2 plunge freezer (2.5 s blot ...
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bioRxiv - Biochemistry 2023Quote: ... gold EM grids (Quantifoil, Jena ...
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bioRxiv - Biochemistry 2023Quote: ... carbon coated copper grids (Cu R2/1, 200 mesh, Quantifoil) and vitrified in liquid ethane using a manual plunge freezing device.
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bioRxiv - Biochemistry 2023Quote: ... 2.8 µl of F-actin sample was applied to a glow-discharged R2/1 Cu 300 mesh holey-carbon grid (Quantifoil). Excess solution was blotted away and the grids were plunge frozen in liquid ethane or a liquid ethane/propane mixture using a Vitrobot Mark IV (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... carbon-coated electron microscopy (EM) grids (200 mesh, Quantifoil) were glow-discharged ...
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bioRxiv - Biochemistry 2023Quote: ... A 3 µL drop was applied to a glow-discharged (PELCO easiGlow, 15 mA, 2 min) holey carbon grid (Quantifoil R1.2/1.3 on gold 200 mesh support), blotted for 3-4 seconds with Whatman Grade 595 filter paper (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... 3 µL of each sample was applied to glow-discharged (PELCO easiGlow, 15 mA, 2 min) holey carbon grid (Quantifoil R1.2/1.3 on gold or copper 200 mesh support) ...
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bioRxiv - Biochemistry 2023Quote: ... the same proteins were applied to holey grids with a 2 nm carbon film (Quantifoil Cu 2/2 200 mesh from EMS). Samples were frozen after a total of 145 min on the grids without carbon film and 150 min on the grids with carbon film ...
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bioRxiv - Biochemistry 2023Quote: ... A sample of 3.0 μM of FH2 and 10.6 μM of actin monomers was applied to holey grids without a carbon film (Quantifoil Au 2/1 300 mesh from EMS). After diluting 4-fold ...