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Citations for Millipore Sigma :
3901 - 3950 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... RMS cells were infected with viral particles for 24h at 37° C using 8 mg/mL of polybrene (EMD Millipore). Cells were selected for using 1 mg/ml G418 (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... washed twice with ice-cold PBS and incubated for 40 minutes at 4°C with 0.25 mg/ml of EZ-Link™ SulfoNHS-SS-Biotin in Hank’s balanced salt solution (Sigma Aldrich). After two further washes with ice-cold PBS ...
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bioRxiv - Microbiology 2022Quote: ... The tissues were deparaffinized by warming the slides at 60°C on a slide warmer for 45 min followed by immersion in xylenes (Sigma) for 25 min ...
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bioRxiv - Physiology 2022Quote: ... containing 150 μL 4°C DMEM (Dulbecco’s modified Eagle’s medium with high glucose and 25mM HEPES from Sigma Aldrich (Merck), UK ...
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bioRxiv - Microbiology 2022Quote: ... The cells were further incubated at 37°C for 72 h before being stained with 1% crystal violet (Sigma-Aldrich).
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bioRxiv - Microbiology 2022Quote: ... Bacteria were grown at 37°C in LB broth with surfactant Pluronic F-109 (Sigma-Aldrich 542342, 425 mg/L) or on plates of LB broth solidified with 1.5% agar (LA-plates) ...
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Structure and Neutralization Mechanism of a Human Antibody Targeting a Complex Epitope on Zika VirusbioRxiv - Microbiology 2022Quote: ... Plates were washed with TBS containing 0.2% Tween and incubated for 1 hour at 37°C with Goat anti-human Fc (Sigma, A9544) or Fab (Jackson immunoResearch AB_2337617 ...
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bioRxiv - Cancer Biology 2022Quote: ... To covalently conjugate the DNA strands to the surface aldehyde groups—thereby converting the hydrolysable Schiff base to single C─N bonds—reductive amination was conducted at room temperature for 15 min in 0.25% sodium borohydride (Sigma-Aldrich) in 1× phosphate-buffered saline (PBS) ...
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bioRxiv - Biophysics 2022Quote: Proteinase K from Tritirachium album (catalog number: P2308) and sialidase from Clostridium perfringens (C. welchii) (catalog number: N2876) were purchased from Sigma.
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bioRxiv - Cancer Biology 2022Quote: ... culture media were frozen at −80°C and tissues were fixed in 10% neutral buffered formalin (#HT501128, Sigma-Aldrich, USA) for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... The re-suspended extract was centrifuged for 5 min at 16000 x g at 4°C and 50 µl of the cleared supernatant was mixed with 25 µl of 100 mM sodium carbonate (Sigma), followed by the addition of 25 µl 2% [v/v] benzoylchloride (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... The imidazole was removed by buffer exchange with base buffer at 4°C in Amicon Ultra-15 filter unit NMWL 3kDa (Millipore).
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bioRxiv - Zoology 2019Quote: ... Finally the homogenate was centrifuged at 10000 rpm for 30 min at 4°c using a cold centrifuge (SIGMA make). An aliquot of 1mL from the clear supernatant was used for the assay of GuDH and protein estimation.
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bioRxiv - Plant Biology 2019Quote: ... The total proteins were extracted by using lysis buffer and then incubated for 3 h at 4°C with 20 ul of Anti-HA Affinity Gel (Millipore). The immunoprecipitates were washed five times with lysis buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... The vDISCO pipeline was started immediately or whole mouse bodies were stored in PBS at 4 °C for up to 4 weeks or in PBS containing 0.05% sodium azide (Sigma, 71290) for up to 6 months.
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bioRxiv - Cell Biology 2019Quote: ... and then incubated overnight at 4°C with anti-BrdU biotin conjugate (1:100, Catalog# MAB3262B, Millipore, Burlington, MA, USA), followed by incubation with Streptavidin Alexa Fluor 488 conjugate (1:800 ...
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bioRxiv - Microbiology 2019Quote: ... and water purified to a resistivity of 18.2 MΩ·cm (at 25 °C) using a Milli-Q Gradient ultrapure water purification system (Millipore, Billerica, MA) were used to prepare mobile phase solvents ...
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bioRxiv - Microbiology 2019Quote: ... Gill tissue pieces were either frozen directly at −80 °C or fixed in RNAlater according to the manufacturer’s instructions (Sigma, Germany) and subsequently frozen at −80 °C.
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bioRxiv - Neuroscience 2019Quote: ... USA] for 12mins at 37 °C and further dissociated by incubating in HBS-/- buffer containing 0.15mg/ml Papain (Sigma, USA) and 10U/ml DNase I (Invitrogen ...
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bioRxiv - Immunology 2019Quote: ... and incubated at 37°C for 2 min before addition of 5 ml of serum-free L-15 containing 1 μg/ml DNase1 (Sigma) for 5 min at 37°C ...
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bioRxiv - Genomics 2019Quote: ... Transfected cells were placed at 25°C and 72 hours after transfection cells were re-suspended in fresh medium supplemented with 5ug/mL of Puromycin (Sigma) and transferred into a well of a 12-well plate ...
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bioRxiv - Plant Biology 2019Quote: ... The resulting SN was concentrated by a factor of about 100 by centrifugation in filter units at 4 °C (Amicon ultra centrifugal filters, 15 mL, 10 kDa MWCO, Millipore). Protein concentration was determined by bicinchoninic acid assay (BCA Protein Assay Kit by Thermo Scientific Pierce) ...
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bioRxiv - Biochemistry 2019Quote: ... were incubated overnight at 4°C with the chromatin extracts and then coupled with 50 μl of protein-G-sepharose beads (GE17-0618-01, Sigma) during 4h at 4°C ...
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bioRxiv - Cell Biology 2019Quote: RAW264.7 cells were loaded with 100 μg/ml AcLDL for 16 h at 37 °C with or without 2 μg/ml ACATi (Sandoz 53-035, Sigma) treatment for 2h ...
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bioRxiv - Cancer Biology 2019Quote: ... and digested at 37°C in Hank’s balanced salt solution containing 2 mg/ml type V collagenase (Sigma-Aldrich, C9263) for 30 min under constant agitation ...
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bioRxiv - Immunology 2019Quote: ... ears were split into dorsal and ventral sides and incubated dermal side down for 35 minutes at 37°C in 0.5 M ammonium thiocyanate (Sigma-Aldrich). Epidermal sheets were gently peeled from the dermis in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were centrifuged at 20,000 × g for 20 min at 4°C and the total protein lysate was quantified using Bradford Reagent (B6916; Sigma). Then ...
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bioRxiv - Cell Biology 2019Quote: ... carrying two isoleucine residues at the N-terminus and a hexahistidine tag at the C-terminus was expressed in Escherichia coli Rosetta2(DE3)pLysS cells (Novagen) and purified by immobilized metal-affinity chromatography followed by gel-filtration chromatography as described previously (Bishop et al. ...
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bioRxiv - Microbiology 2019Quote: ... This fragment was cloned by ligation independent cloning into the linearized pIEX-10 EK-LIC vector which carries a C-terminal 10xHis tag (Novagen) to generate pIEX-10 ...
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bioRxiv - Microbiology 2019Quote: ... was cloned into the protein expression vector pET28a (+) containing an IPTG-inducible promoter and a C-terminal 6x-His tag (Novagen). Expression of CosR-His was then induced in E ...
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bioRxiv - Developmental Biology 2020Quote: ... HA-tagged or Myc-tagged proteins were isolated with anti-HA agarose or anti-c-Myc Agarose Affinity Gel (Sigma), respectively ...
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bioRxiv - Synthetic Biology 2020Quote: ... Single colonies were grown overnight at 37 °C in 3 ml of LB (Hy-Labs) supplemented with 100 μg/ml ampicillin (Sigma). Plasmid DNA was purified using QIAprep spin miniprep kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... used for barrier dysfunction experiments were conducted on a 37 °C heating block using a Millicell ERS-2 Voltohmmeter (Merck-Millipore) equipped with an Ag/AgCl electrode (STX01) ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1 M NaHCO3) and cross-linking was reversed overnight at 65°C with 200 mM NaCl and 2 μl RNase A (Sigma). Matched input samples (10% of original ChIP reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.2mg Dynabeads-Protein-A was blocked overnight at 4°C in 1X IP buffer supplemented with 0.5mg ml-1 BSA (Sigma A7906). The antibody-RNA mixture was split into 50µl aliquots on ice and crosslinked with 0.15J cm-2 254nm UV radiation six times ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were maintained at 37°C in 5% CO2 atmosphere and treated with 2 µg/ml tunicamycin (Sigma-Aldrich), 10 nM-1µM thapsigargin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were centrifuged 20 minutes at 4°C at 45000 g and the supernatants were incubated with glutathione-coupled Sepharose 4B beads (Sigma) for 30 minutes at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... This was followed by a 16 hour incubation at 4 °C in a 1:1000 dilution of BIII-tubulin (Sigma) raised in mouse ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial strains were grown in glass test tubes (18 × 150 mm) at 35°C in cation adjusted Müeller-Hinton Broth II (MHB, Sigma-Aldrich) with agitation at 210 revolutions per minute (RPM) ...
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bioRxiv - Immunology 2020Quote: ... Macrophages were then harvested by incubation (20 min at 4°C) with cold PBS (without CaCl2 and MgCl2, Sigma-Aldrich). Macrophages were then flushed off the plate ...
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bioRxiv - Bioengineering 2020Quote: ... the brain was kept in 4% PFA overnight at 4□°C and then placed into PBS containing 0.02% sodium azide (Sigma S8032). The brain was serially sectioned into 50 μm transverse slices on a Campden Instruments 5100mz vibratome ...
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bioRxiv - Plant Biology 2021Quote: ... Fixed plant material was digested for 0.5 to 1.5 hours at 37° C in an enzyme mixture consisting of 2 % (w/v) cellulase from Aspergillus niger (Sigma C1184), 4 % (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... were expressed as fusion proteins with 6x polyHis tail at the C-terminus in E.coli strain BL21(DE3) (Merck-Novagen, Darmstadt). After harvesting ...
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bioRxiv - Plant Biology 2020Quote: ... stratified for 2 d at 4°C in the dark and sown on Gamborg’s B5 Basal Salt medium (Sigma G5768) supplemented with 1% (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... then cured for 5 min at 120°C and further spin-coated (30 sec., 1000 rpm) with 1% polystyrene (Sigma) in toluene (Sigma) ...
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bioRxiv - Microbiology 2019Quote: ... were coated overnight at 4°C with 50 µl of partially purified mucins from bovine submaxillary glands (type I-S; Sigma) and porcine stomachs (type II and III ...
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bioRxiv - Pathology 2020Quote: ... 150 mM NaCl containing the corresponding detergents and bound proteins were eluted three times with 10 μL 0.5 mg/mL c-Myc peptide (Sigma M2435) in the corresponding detergent containing buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The cell line was cultured at 37 °C under 4.5 % CO2 in EX-CELL® 293 Serum-Free Medium for HEK 293 Cells (Sigma), supplemented with 4 mM L-glutamine ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HUVEC cells were seeded in 6 well plates at a cell density of 160,000 cells/well and maintained at 37°C in 5% CO2 with Complete Endothelial Cell Growth Media containing 100μg/ml streptomycin (Sigma, US). 24hrs after seeding ...
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bioRxiv - Biochemistry 2021Quote: ... coli B834(DE3) carrying pET28b(+)-phm7 was cultured at 18 °C for 96 h using the Overnight Express Autoinduction System 2 (Novagen). Cells expressing the DS were harvested by centrifugation and frozen at –80 °C until use.