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Citations for Millipore Sigma :
3951 - 4000 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Cells were incubated at 37°C for 15 days and were then fixed and stained with crystal violet (Sigma-Aldrich). Colonies were counted using an in-house–built ImageJ macro using a Trainable Weka Segmentation plugin ...
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bioRxiv - Microbiology 2019Quote: ... 4°C) and resuspended in binding buffer (50 mM Tris, 1 mM MgCl2, 2 mM imidazole, 1 mM PMSF (Sigma), 300 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Mosquito homogenates were clarified by centrifugation at 20,000×g (4 °C for 30 min) and filtered through a 0.45 μm membrane filter (Millipore, Billerica, USA). Supernatants were collected and stored at −80 °C ...
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bioRxiv - Microbiology 2021Quote: ... Samples with the highest purity were pooled together and concentrated by centrifugation at 4°C with Amicon Ultra Centrifugal Filter Devices of 10 000 MWCO (Millipore). All protein versions were purified at least twice to ensure reproducibility of the results ...
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bioRxiv - Microbiology 2021Quote: ... Cells were returned to neutral pH and either incubated at 37°C in the presence of lyso-PC or washed with delipidated BSA (2 mg/ml, Sigma) to remove lyso-PC and then maintained at 37°C for 30 min.
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bioRxiv - Microbiology 2021Quote: ... Cells were re-suspended in RPMI before undergoing centrifugation at 2,000 rpm for 20 min at 4°C over Histopaque 1077 (Sigma-Aldrich). Bursal cells that banded at the interface of the medium and histopaque were collected ...
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bioRxiv - Microbiology 2021Quote: All colicin constructs were conjugated to a 6xHis tail at their C-terminus and cloned at the second multiple cloning site of the apACYCDuet-1 (Novagen) plasmid where they were expressed under a T7 promotor ...
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bioRxiv - Microbiology 2021Quote: ... and the metal-loaded polymer purified and washed in C-buffer using an Amicon Ultra-0.5 centrifugal filter unit with 3kDa cutoff (Millipore-Sigma). At the same time ...
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bioRxiv - Genetics 2020Quote: ... Bead-beating was done in an Axygen 1.1 ml plate (P-DW-11-C-S) with 250 μl of acid washed 425–600 μm beads (Sigma G8722) per well ...
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bioRxiv - Neuroscience 2019Quote: ... Hippocampi were isolated and digested for 30 min at 37°C in 10mM glucose (500 µL/brain) containing 25U of papain (Sigma) and 20 µg/mL DNAse (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... were taken from normal and saline injected skin (6 or 24-hours post-injection) and disaggregated by overnight incubation (37°C) in either 0.8 mg/ml collagenase IV (Sigma Aldrich) with 20% FCS or a whole skin digestion kit from Miltenyi Biotec (Cambridge ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for 24 hours at 4 °C in mouse anti-GAD67 (1:1000; Millipore Sigma, cat# MAB5406), rabbit anti-NeuN (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Aliquots of BCG cultures at an OD600 of 0.8 were stored at −80°C in RPMI-1640 media supplemented with 10% glycerol (Sigma-Aldrich). The number of viable bacteria was assessed by thawing a frozen aliquot and plating serial dilutions onto Middlebrook 7H11 (BD Pharmingen ...
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bioRxiv - Genomics 2021Quote: ... at room temperature with a thick layer of self-indicating silica gel (a granular material with c. 3 to 5mm irregular beads; e.g. Type III Sigma-Aldrich, S7625 ...
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bioRxiv - Biophysics 2019Quote: ... Cells were first rinsed three times with pre-warmed (at 37 °C) phosphate buffered saline (PBS, 806552-500ML, Sigma-Aldrich) and then fixed for 15 minutes at room temperature (RT ...
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bioRxiv - Biochemistry 2019Quote: Pellets were thawed in a water bath at 37°C and resuspended in 50 mM HEPES-KOH pH 8.5 (Sigma-Aldrich), 50 mM KCl (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2019Quote: Pellets were thawed in a water bath at 37°C and resuspended in 50 mM HEPES-KOH pH 8.5 (Sigma-Aldrich), 50 mM KCl (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2019Quote: Small RNAs were prepared from untreated and UV-C treated plants (time points 0 and 24h) using the Tri-Reagent (Sigma), used for library preparation and sequencing by Illumina Hi-Seq (FASTERIS ...
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bioRxiv - Developmental Biology 2019Quote: ... The preparation was transferred to a microscope stage and perfused at room temperature (1ml per min, 20–24°C) with an aerated recording solution that contained 15 μM d tubocurarine (Sigma) to paralyze the preparations ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were cultured in SILAC DMEM Medium (PAA Laboratories) at 37°C and 5% CO2 and complemented with 10% dFCS (Sigma), 1% Penicillin-Streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2019Quote: ... ANE was filtered through cotton to remove the insoluble materials as much as possible and then centrifuged at 2,900 g for 30 min at 4°C with 100 kDa-pored concentration tube (Z677906, Sigma-Aldrich). The flow-through fraction was collected and further subjected to centrifugation as above with 30 kDa-pored concentration tube (430-30 ...
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bioRxiv - Microbiology 2019Quote: ... The cultures were incubated for an additional 4 h at 37°C and the media was replaced with microscopy-grade media (Sigma DMEM without Phenol Red ...
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bioRxiv - Systems Biology 2020Quote: ... The hydrolysates were dried at 105°C and the dried pellets resuspended in 20 µL of dimethylformamide (DMF, Sigma Aldrich). The amino acids were derivatised adding 20 µL of N-tertbutyldimethylsilyl-N-methyltrifluoroacetamide (TBDMSTFA ...
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bioRxiv - Molecular Biology 2020Quote: ... or heat stress (42°C in L-15 Leibovitz medium in CO2-free environment) and co-treated with DMSO (0.1%, Sigma-Aldrich), MG132 (10 μM in DMSO ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were then incubated overnight at 4°C with rabbit anti-myc (1:250; Sigma-Aldrich Cat# C3956, RRID:AB_439680) in PBS with 2% BSA ...
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bioRxiv - Microbiology 2020Quote: ... Lungs from intranasally immunized mice were harvested and digested for 1 h at 37°C in digestion buffer consisting of RPMI media supplemented with (167 μg/ml) of Liberase DH (Sigma) and (100 μg/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... All cells were grown in a humidified chamber at 37°C supplied with 5% CO2 in media supplemented with 10% fetal bovine serum (FBS; Millipore), 1% Penicillin/Streptomycin antibiotics (Corning) ...
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bioRxiv - Genetics 2020Quote: ... Cells were pre-warmed to 37°C for 5 minutes before incubation with 1 U/ml of MNase (Sigma, #N3755) for 15 minutes at 37°C with mixing at 800 rpm in an Eppendorf Thermomixer ...
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bioRxiv - Genetics 2020Quote: gDNA was prepared from sperm by washing epididymides in PBS at 37 °C followed by transfer to the centre well of a 37 °C Center-Well Organ Culture Dish (Falcon) containing 500 µL of M2 medium (Sigma) in the centre well and 3 mL of PBS in the outer well ...
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bioRxiv - Genetics 2020Quote: ... thawed sections were incubated in cytochrome c medium (100mM cytochrome c, 4mM diaminobenzidine tetrahydrochloride, 20μg/ml catalase in 0.2M phosphate buffer, pH 7.0; all from Sigma Aldrich, Poole, UK) for approximately 50min at 37°C or until satisfactory visualization of the brown color ...
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bioRxiv - Bioengineering 2021Quote: ... at −20°C for 6 minutes followed by a 1-hour incubation in blocking buffer containing 10 wt% BSA (Sigma) in PBS as previously described7 ...
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bioRxiv - Physiology 2021Quote: ... without neurotrophic factors supplemented with 100 μM deferoxamine (DFO concentration determined based on dose curve at 10°C) (Sigma-Aldrich), 100 nM BAM15 (Tocris ...
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bioRxiv - Microbiology 2021Quote: ... at 25,000 x g for 2 h at 4°C on a 20% sucrose cushion (Sigma-Aldrich, St. Louis, MO). Media and sucrose were discarded ...
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bioRxiv - Immunology 2020Quote: ... samples were centrifuged at 22000 x g for 20 minutes at 4 °C and the supernatant was filtered through Millex GV 220 nm filter (Merck-Millipore). For acid treatment acetic acid was used with 0.1 N final concentration ...
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bioRxiv - Developmental Biology 2020Quote: Adult females collected 24-48h after eclosion were fed on yeast at 25°C for two days before dissecting ovaries in M3 insect medium (Sigma). Egg chambers were transferred into 4% paraformaldehyde in M3 medium/PBS and fixed at room temperature for 20 min ...
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bioRxiv - Cell Biology 2021Quote: 5 mL of yeast cells expressing the indicated constructs (see main text) were grown at 30°C in liquid YNB media (6.8 g/L) (Sigma-Aldrich) supplemented with 5 g/L glucose and 1.92 g/L synthetic drop-out medium without uracil (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... Flagella preparations were incubated at 70°C for 15 min then filtered by centrifugation at 5000 × g at 4°C for 30 min using 100 kDa 4 ml filter units (Millipore). Filtrates were confirmed by size-exclusion HPLC to be exclusively monomeric.
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bioRxiv - Cell Biology 2020Quote: ... and media were continuously flowed at 1 psi while heating at 32 °C by a temperature controllable manifold (CAX2-MXT20; Millipore). For confocal imaging ...
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bioRxiv - Biophysics 2021Quote: ... Reactions were incubated at 30°C and at the indicated times 2 μL were spotted on TLC PEI Cellulose F (Millipore) to stop the reaction ...
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bioRxiv - Cell Biology 2019Quote: ... After 24°C of transfection cells plated in gold fiducial coverslip were fixed with 0.8% PFA and 0.1% glutaraldehyde (Sigma G7526-10ML) solution (in PBS ...
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bioRxiv - Cancer Biology 2021Quote: 1 μg genomic DNA was pretreated with sodium hydroxide for 15 minutes at 37 °C followed by incubation with hydroquinone (Sigma) and sodium metabisulfite (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed once with PBS prior incubation for 5 min at 37 °C with 4′,6-diamidino-2-phenylindole (DAPI; Sigma). Finally ...
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bioRxiv - Biochemistry 2021Quote: ... 2-3 mg of sNASP-FLAG-myc and sNASP-Strep-HA extracts were incubated for 3 hours at 4 °C degrees with 50 μl of anti-Flag M2 (A2220, Sigma) or Anti-TwinStrep Mag-Strep beads (2-4090-002 ...
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bioRxiv - Biochemistry 2021Quote: ... were coated with 10 mM DOPA-Tet and incubated for 5 days at 37 °C in 1 mL of either 50 mM MES pH 4.5 (Millipore-Sigma), 100 mM MES pH 6.0 ...
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bioRxiv - Biochemistry 2021Quote: ... Two equivalents of linker were combined with one equivalent of c(RGDfK) in 50 mM HEPES pH 8.0 buffer (Millipore-Sigma) and incubated at RT for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... Fractions containing eluted BfNFeoAB were concentrated at 4°C using a 15 mL Amicon 3 kDa molecular-weight cutoff (MWCO) spin concentrator (Millipore). Concentrated protein was then further purified by size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2021Quote: ... The second half of the clarified lysate was incubated for 2 h at 4°C with 20 μl of Anti-FLAG M2 magnetic beads (Sigma) equilibrated in FLAG wash buffer containing 20 mM HEPES pH 8.0 ...
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bioRxiv - Immunology 2021Quote: ... at 4°C overnight and then blocked with 10% fetal calf serum at 4°C overnight followed by six washes with PBS containing 0.2% Tween 20 (Sigma-Aldrich). Prepared lectin plates were then incubated at 4°C overnight with a transmembrane deleted form of the MARV GP Popp strain (Lake Victoria MARV ...
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bioRxiv - Microbiology 2021Quote: ... tissues were incubated until dissolved at 37°C in 495 μL extraction buffer [(4 M urea, 0.2 M NaCl, 1 mM trisodium citrate, 1% SDS (Sigma-Aldrich)] supplemented with 5 μL Proteinase K (20 mg mL−1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Obtained complemented strains were grown at 37°C for 16 h in BHI supplemented or not with 1 mM of IPTG (Sigma) until stationary phase was reached ...