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Citations for Millipore Sigma :
4001 - 4050 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 10 or 16 J/m2 or locally irradiated with a UV-C dose of 60 or 100 J/m2 through a filter with holes of 5 μm of diameter (Millipore). After irradiation ...
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bioRxiv - Biochemistry 2021Quote: ... Proximity ligation reaction was followed by O/N decrosslinking at 65°C in the presence of 250 μg/ml proteinase K (Eurobio) and 5 mM EDTA (Sigma).
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bioRxiv - Pathology 2021Quote: ... at 25,000 x g for 2 hours at 4 °C on a 20% sucrose cushion (Sigma-Aldrich, St. Louis, MO). Media and sucrose were decanted ...
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bioRxiv - Microbiology 2021Quote: ... Bacteria were grown aerobically at 37 °C in Middlebrook 7H9 medium supplemented with 250 µg/mL Hygromycin B (Sigma-Aldrich) and harvested after 72 h by centrifugation followed by resuspension in ice-cold PBS containing 1 mM MgCl2 and CaCl2 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... Sorted 96-well plates were frozen at −80 C until processing when cells were lysed with 0.2 µL 1 M KCl (Millipore Sigma), 0.2uL 10% Triton X-100 (Millipore Sigma) ...
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bioRxiv - Cancer Biology 2021Quote: Cells were fixed in 70% ethanol for at least 2 hrs at −20°C and resuspended in a PBS solution containing 35 ug/ml propidium iodide (Sigma) and 100 ug/ml RNAse A (Roche) ...
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bioRxiv - Developmental Biology 2021Quote: ... inactivated with 10 µg/ml mitomycin C and seeded at 4 × 105 per 6 cm dish) in DMEM/F12/KSR medium (D-MEM/F12 (Sigma) supplemented with 20% KSR additive ...
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bioRxiv - Biophysics 2020Quote: ... Syn7942 cells were incubated at 30°C with 25 mM NaH14CO3 for 2 minutes before permeabilization with 0.03 % (w/v) mixed alkyltrimethylammonium bromide (Sigma-Aldrich) and RuBP (Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... KG1a cells were first collected and treated for 20 min at 37°C with bromelain (250 μg ml-1; Sigma) in RPMI media with 10% FBS ...
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bioRxiv - Cell Biology 2020Quote: ... confluent EnSC monolayers were down-regulated in phenol-free DMEM/F□12 media containing 2% DCC□FBS and decidualized with 10 μM medroxyprogesterone acetate (MPA) and 0.5 mM 8 bromo cAMP (C+M treatment) (Sigma Aldrich). To study the effect of exogenous HA ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were permeabilized and stained for 15 minutes at 37 °C with a solution containing 500 µg/mL PI (Sigma) in PBS with 0.1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... then heated for 5 min at 120°C and spin-coated again for 30 sec at 1000 rpm with 1% polystyrene in toluene (Sigma). Coverslips were then oxidized by plasma (FEMTO ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma], 10 mM NaCl [Sigma] ...
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bioRxiv - Cell Biology 2020Quote: ... the other four fractions were incubated at 37°C for either five minutes or thirty minutes with combinations of Proteinase K (0.01 µg/mL, Sigma-Aldrich) and/or IGEPAL (0.1%) ...
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bioRxiv - Synthetic Biology 2021Quote: ... were thawed in a water bath at room temperature and buffer exchanged into sterile water chilled to 4 °C using 0.5 mL 30 kDA molecular weight cutoff devices (Millipore Sigma). A volume of protein corresponding to 10 μg total Donkey1.1 was boiled at 100 °C for 5 min and then cooled to room temperature on the benchtop ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were then solubilized overnight at 4°C in presence of 0.5% n-Dodecyl β-D-maltoside (DDM) (Sigma, D4641). Lysates were used for co-immunoprecipitation using Dynabeads M-280 Streptavidin (ThermoFisher ...
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bioRxiv - Genomics 2020Quote: ... and placed in gentleMACS c-tubes (Miltenyi 130-096-334) with digestion buffer (400 µg/ml Liberase DL (Sigma 5466202001) in RPMI (Gibco 72400120)) ...
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bioRxiv - Immunology 2020Quote: ... The head kidney and spleen of each animal were dissected out and kept at 4 °C in high glucose Dulbecco’s Modified Eagle Medium (DMEM) (Sigma-Aldrich) and 0.2% Primocin™ (InvivoGen ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.1 mg/ml of purified PIM1 and PIM1 mutants were incubated at 37 °C with or without 0.08 mg/ml beta-casein (Sigma-Aldrich). Aliquots were taken at specified time intervals and immediately mixed with 6X SDS sample buffer to terminate the reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... thereafter named HEK293) were cultured under 5% CO2 at 37°C in low-glucose Dulbecco’s modified Eagle’s medium (DMEM) (Sigma–Aldrich, D6046) supplemented with 10% foetal bovine serum (FBS-Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: ... subjected to freeze/thaw at -80°C, and sonicated in lysis buffer (1x PBS, 1% CHAPS, 10mM MgCl2, 2 µl benzonase (25 U/µl, Millipore), and protease inhibitor (Thermo Scientific)) ...
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bioRxiv - Microbiology 2020Quote: MDCK (Madin-Darby canine kidney, obtained from ATCC) were grown at 37°C in 5% CO2 in MEM medium (Sigma) containing 5% Fetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... After 24 hrs the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore). The HeLa cells stably expressing mouse Cx26 were originally obtained from Dr K ...
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bioRxiv - Microbiology 2020Quote: ... albicans was first grown overnight to an OD600 of approximately one in YPD at 30°C and was then treated with 1.6 mM H2O2 (Sigma-Aldrich) for 15 minutes while shaking at 30 °C ...
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bioRxiv - Microbiology 2020Quote: ... chondrophila were first evidenced by incubating the purified recombinant enzymes overnight at 30°C with 10 mg.mL-1 glycogen from rabbit liver (Sigma-Aldrich) and 20 mM inorganic phosphate in 20 mM Tris-HCl buffer (pH 6.8) ...
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bioRxiv - Immunology 2021Quote: ... 2.33×108 4T1cells grown in culture or 8×108 grown in vivo in BALB/c mice per replicate were lysed in phosphate buffered saline containing 0.5% sodium deoxycholate (Sigma-Aldrich), 0.2 mM iodoacetamide (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... coli strains were cultivated at 37°C in Luria-Bertani medium with shaking at 37°C in the presence of 0.3 mM thymidine or diaminopimelic acid (Sigma-Aldrich), respectively ...
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bioRxiv - Immunology 2020Quote: ... and differentiated at 37°C in 5% CO2 using 20 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma, P8139) for 24 h ...
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bioRxiv - Immunology 2020Quote: ... the protein transferred membranes were blocked with 5% dry milk in PBST (PBS with 0.05% tween-20) overnight at 4°C and probed with goat anti-human IgG-HRP (Sigma-Aldrich, St ...
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bioRxiv - Immunology 2021Quote: ... Tissues were then rinsed in cold PBS and digested for 50 minutes at 37°C in complete medium (10% FBS, 100 U/ml, penicillin/streptomycin (P0781 Sigma) in RPMI-1640 ...
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bioRxiv - Immunology 2020Quote: ... cells were re-stimulated ex vivo for 4h at 37°C with 0.5 μg/ml of SIINFEKL peptide and 10 μg/ml of Brefeldin A (Sigma-Aldrich) in complete RPMI ...
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bioRxiv - Microbiology 2021Quote: The c-di-GMP levels were calculated from a standard curve generated from pure c-di-GMP suspended in 184 mM NaCl (Sigma) at 0 ...
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bioRxiv - Immunology 2021Quote: ... tissue pieces remaining after the DTT/EDTA step were chopped and incubated for 40 min at 37°C with Collagenase D (2 mg/ml; Sigma) and DNase (1 mg/ml ...
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bioRxiv - Developmental Biology 2020Quote: Spinal cords were incubated at 38°C for 20 minutes with F12 medium supplemented with 5% FCS (F7524; Sigma-Aldrich), 20mM HEPES buffer (15630-049 ...
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bioRxiv - Immunology 2021Quote: ... Arrays were sealed in a dry 37°C oven for 40 min and submerged in a lysis buffer containing guanidium thiocyanate (Sigma), EDTA ...
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bioRxiv - Cell Biology 2021Quote: ... was further digested by incubating at 37°C for 60 min at 200 rpm with 2000 U of collagenase (Sigma). The digested tissue was then pelleted and lysed at 47ºC for 5 minutes using ACK lysis buffer (Gibco) ...
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bioRxiv - Genomics 2021Quote: ... cells were washed once in 37°C warm PBS-buffer immediately followed by the addition of 3.5 mL of freezer-cooled (−20 °C) LC-MS grade 80:20 MeOH/H2O (Sigma Aldrich). The plates were then held at −20 °C for 2 hours ...
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bioRxiv - Genetics 2019Quote: ... Hemocytes were washed (three times, 15 minutes each at 4 °C) in PBS before overnight staining with DAPI 1X (405 nm, Sigma) and Phalloidin 1X (488 nm ...
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bioRxiv - Genomics 2021Quote: ... Tissue was incubated in solution at 90°C for 30 minutes followed by three washes in 0.1M TBS (Trizma Base (Sigma T1503), NaCl (Sigma S9888) ...
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bioRxiv - Genomics 2021Quote: ... Three mL of samples were first denatured with SDS to a final concentration of 1% (wt/vol) and 2.7 mL of preheated acid phenol (65°C) (Sigma-Aldrich). Mixtures were vortexed for 5 min at 65°C ...
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bioRxiv - Genomics 2021Quote: Fresh nuclei were incubated for 3 minutes at 37°C with limiting concentrations of the DNA endonuclease deoxyribonuclease I (DNase I) (Sigma) in buffer A supplemented with Ca2+ ...
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bioRxiv - Cell Biology 2021Quote: ... and with 10mM EDTA then incubated with Goat anti plexin-A2-APC (RαD systems) for 40 min in the dark at 25 °C for staining plexin-A2 or with Mouse anti-FLAG (Sigma) for 40 min at 4°C followed by Goat anti mouse IgG H&L (Alexa Fluor®647)(abcam ...
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bioRxiv - Cell Biology 2021Quote: Cell supernatant was collected 24h after changing cell medium then immediately filtered by centrifugation for 15 minutes at 4°C on a 10KDa column (Abcam or Millipore). This step eliminates proteins ...
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bioRxiv - Microbiology 2021Quote: ... NTHi bacteria were cultured at 37° C on brain heart infusion agar (Difco, NJ, USA) supplemented with nicotinamide adenine dinucleotide (10 μg/ml, Sigma) and hemin (10 μg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were washed with cold PBS and incubated with 200 μl of DNase-free RNAse A in PBS (100 μg/ml) for 30 min at 37°C before DNA staining with 200 μl of Propidium Iodide (PI, Sigma) (50 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... total mononuclear cells isolated from the epithelium were plated in 48-well plates and incubated at 37° C with 100ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma), 200ng/mL ionomycin (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... lentiviruses were thawed at 37°C and added to target cells in complete media containing 5 µg/mL polybrene (Sigma) for 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates were then incubated for 60 min at 4 °C with anti-FLAG M2 Affinity Gel (A2220, Sigma-Aldrich). The beads were washed three times with immunoprecipitation wash buffer [50 mM HEPES-NaOH (pH 7.2) ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were then incubated for 1 hr at 37°C before being fixed by resuspending in YP + 1% formaldehyde (Sigma) for 45 min at 25 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Following electrophoresis, proteins were transferred (100 V, 2 hr, 4°C) to polyvinylidene difluoride membranes (Immobilon-FL, Millipore, Billerica, MA). To confirm protein transfer ...