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Citations for Millipore Sigma :
3801 - 3850 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... single-cell suspensions of epidermal cells were obtained from ear skin and incubated for 1 hour at 37 °C in 0.3 % trypsin (Sigma-Aldrich) in 150 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were maintained at 37 °C for 7 d to allow colony formation before stained with 0.5% crystal violet (Sigma-Aldrich) in absolute methanol ...
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bioRxiv - Bioengineering 2020Quote: ... and enzyme-digested tissues were triturated in 10 mL of warm (37°C) Dulbecco’s Modified Eagle Medium (DMEM, Sigma-Aldrich) containing 10% heat-inactivated FBS ...
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bioRxiv - Biophysics 2021Quote: ... The virus-containing supernatant was concentrated by ultracentrifugation in a SW-28 rotor (25,000 rpm, 2 h, 4°C) using OptiPrep density gradient medium (Sigma-Aldrich). Part of the supernatant containing pelleted viruses was collected ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed three times in PHEM buffer preheated to 37° C (PHEM buffer: 60mM PIPES, 25mM HEPES, 5mM EGTA, 2mM Mg (CH3CO) 2 (Sigma), pH6.9) ...
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bioRxiv - Cell Biology 2020Quote: ... Mitochondrial lysates were cleared by centrifugation at 20,000× g at 4°C and transferred to pre-equilibrated anti-FLAG M2 agarose beads (Sigma-Aldrich) for FLAG immunoprecipitation or onto protein A-Sepharose (PAS ...
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bioRxiv - Cell Biology 2020Quote: ... using N2B27 2i + Lif medium at 37°C and 7% CO2 in culture flasks coated with 0.1% gelatine (Sigma-Aldrich, #G1890 SIGMA) diluted in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were collected and either snap frozen in liquid nitrogen and stored at −80°C for later biochemical analyses or processed by overnight immersion in freshly prepared fixative: 3.7% Paraformaldehyde (Sigma, P6148), 200 mM HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... astrocytes were generated by shaking confluent mixed-glial cultures at 10-14 days in vitro (DIV) at 180 rpm overnight to remove microglia followed by 7 days of treatment with cytosine arabinoside (Ara-C, Sigma). To generate microglia ...
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bioRxiv - Cancer Biology 2022Quote: ... minced pancreata were digested at 37°C for 30 minutes in a dissociation buffer containing 0.05% collagenase P (Sigma-Aldrich) and 0.1% DNase I (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... fumigatus was grown for 5 days at 37 °C and conidia were harvested and labeled fluorescein isothiocyanate (FITC; Sigma-Aldrich) or calcofluor white (CFW ...
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bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
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bioRxiv - Biophysics 2022Quote: ... coli optimized gene sequence for expression of S.c. Cdc6 in E. coli (a generous gift of Dr. Megan Davey, Western University, Canada) was cloned into pET11 (Novagen). The Cdc6 gene was then subcloned into pGEX-6P-1 (GeneScript ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 µg ml−1 of L-ascorbic acid or 2-Phospho-L-ascorbic acid trisodium salt (Vitamin C) (Sigma). NSCs were cultured in NSC complete medium consisting of DMEM/F-12 Media ...
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bioRxiv - Biochemistry 2022Quote: ... the protein samples were reduced with 20 mmol/L dithiothreitol at 37 °C for 1 h and alkylated with 50 mmol/L iodoacetamide (IAA, Sigma) for 45 min in the dark ...
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bioRxiv - Biochemistry 2022Quote: ... Immobilized pepsin was removed by centrifugation at 1,000 x g for 30 sec at 0 °C using a 0.65 µm filter inlet (Merck Millipore, Darmstadt, Germany) and the flow through was injected immediately into the HPLC-MS/MS system ...
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bioRxiv - Biochemistry 2022Quote: ... The protein content was extracted from the Mitra devices by 1-hour incubation at 60° C in 35% TFE buffer containing 40 mM dithiothreitol (DTT; Sigma) and 50 mM ammonium bicarbonate (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA tagged with 6xHis at the C-terminus was introduced into a pNG2 vector (a derivative of pET-3a, Merck-Novagen) linearized with NdeI and BamHI ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for 24-40h at 4°C in mouse anti-GAD67 (1:1000; Sigma Millipore, catalog #MAB5406), rabbit anti-NeuN (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated for 24-40h at 4°C in mouse anti-GAD67 (1:1000; Sigma Millipore, catalog #MAB5406), rabbit anti-NeuN (1:500 ...
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bioRxiv - Systems Biology 2022Quote: ... Protein precipitation was performed by addition of four volumes of ice-cold acetone (−20°C) (Merck Sigma, Cat. No. 650501) or TCA to a final concentration of 20% and proceeded for 1 hour at -20°C or 30 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Surface staining was then performed for 45 minutes at 4°C in PBS (TFS) supplemented with 1\% FBS (BioWest) and 0.09\% NaN3 (Sigma-Aldrich). Cells were then fixed and permeabilized in 96 wells plates using 200 uL of BrdU staining solution from the BrdU Staining Kit for Flow Cytometry APC (ThermoScientific ...
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bioRxiv - Microbiology 2022Quote: ... dialyzed in buffer C and then concentrated in a centrifugal concentrator with a 10-kDa molecular weight cutoff membrane (Merck Millipore).
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bioRxiv - Systems Biology 2022Quote: ... 3- Supernatant was discarded and 2 ml of prewarmed (35 °C) collagenase-trypsin solution (0.55 mg/ml collagenase, 0.37 mg/ml Trypsin (Sigma, cat. # T0303), and 60 U/ml TurboNuclease in Hank’s balanced salt solution ...
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bioRxiv - Neuroscience 2022Quote: ... High Five™ insect cells for the expression of PP2A A/C complex were cultured in EX-CELL 405 Serum-Free medium (Millipore-Sigma) at 27°C with 130 rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated at 30°C and at the indicated times 2 μL aliquots were spotted on TLC PEI Cellulose F (Millipore) to stop the reaction ...
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bioRxiv - Microbiology 2022Quote: ... Transfected parasites were incubated overnight at 37 °C and 5% CO2 with media being replaced with complete DMEM containing 25μg/mL MPA (Sigma-Aldrich) and 50μg/mL xanthine (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were centrifuged at 21,130g for 15 minutes at 4°C and supernatants were added to 50 μl of M2 anti-FLAG-agarose beads (Sigma). Beads were washed 4 times with NET-2 (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged 1800 rpm for 6 minutes at 4°C and pellets were resuspended n 3 mL RBC lysis for 4 minutes (Sigma). Samples were centrifuged 1800 rpm for 6 minutes at 4°C and the pellets were resuspended in 200 μl of PBS + 2% FBS + 2 mM EDTA ...
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bioRxiv - Neuroscience 2022Quote: Headless larvae (7 dpf) were fixed for three days at 4 °C in EM fixation buffer [2% EM-grade glutaraldehyde (Sigma), 2% PFA (Sigma) ...
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bioRxiv - Genomics 2022Quote: ... The mycelium was washed in SMC and incubated for 4 h at 37 °C in lysing enzymes from Trichoderma harzianum (Sigma) dissolved in SMC ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were centrifuged 15 min at 13200 rpm at 4°C and protein concentration measured by Bradford assay (Sigma-Aldrich).
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bioRxiv - Immunology 2022Quote: ... The supernatant was then concentrated to 5% of its original volume using an Amicon Ultra with a 100 KDa cutoff at 16 °C (Merck Millipore). HEK-293 cells stably expressing hACE2 were seeded into 0.1% gelatin-coated 96-well plates (Greiner ...
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bioRxiv - Cell Biology 2022Quote: ... and treated for 4 h at 37°C in a polymerase solution containing fluorescent nucleotides as described (Sigma-Aldrich, Duolink). Samples were then washed 2x with buffer B (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... Fixed cells were pelleted and incubated overnight at 50 °C in 500 µL Sodium Citrate (50 mM) with 0.7 mL RNase A (30 mg/ml; Sigma-Aldrich). Cell suspensions were treated with proteinase K (20 mg/ml ...
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bioRxiv - Biophysics 2022Quote: Myristoylated c-Src constructs were obtained by co-expression of the N-myristoyl transferase (NMT) enzyme along the c-Src USH3-His6Tag construct in a pETDuet-1 (Novagen) plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1h at 37°C and then with 20μL 1% tert-butyldimethylchlorosilane in N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide (Sigma Aldrich) for 3h at 60°C ...
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bioRxiv - Cell Biology 2022Quote: ... in pyridine at 70°C for 15 min followed by addition of N-tert-Butyldimethylsiyl-N-methyltrifluoroacetamide (MTBSTFA, Sigma-Aldrich) for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... membranes were equilibrated at 4 °C and incubated for 30 min in the presence of 2 mg/ml iodoacetamide (Sigma). Membranes were then solubilized in 10 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... Adult mice were engineered with floxed alleles of Atg7 and a transgene expressing the TAM-regulated Cre recombinase fusion protein under the control of the ubiquitously expressed ubiquitin C (UBC) promoter.15 Atg7 deficiency was created by providing tamoxifen (Sigma Cat No ...
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bioRxiv - Cell Biology 2022Quote: The tissue section on the Stereo-seq chip (5 cm x 3 cm or 2 cm x 3 cm) was then incubated at 37°C for 5-8 min and subsequently fixed in methanol (Sigma, 34860 ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated at 37°C for 30 minutes and then the desired culture media was added supplemented with 10.5 µM Y-27632 (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... Mock- and virus-infected cells were harvested at 16 h post infection and stored at −80°C in RNALater (Sigma) until RNA extraction ...
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bioRxiv - Neuroscience 2020Quote: ... boiled at 95°C for 5 min and subjected to 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride (PVDF) (Millipore) at 20 V/cm for 1h ...
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bioRxiv - Plant Biology 2021Quote: ... tumefaciens GV3101 strains harboring expression constructs of interest were pre-incubated at room temperature (ca. 22°C) in induction media (10 mM MES pH 5.6, 10 mM MgCl2, 150 mM acetosyringone [Sigma-Aldrich]) for 2 h before hand-infiltration into 5-week-old N ...
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bioRxiv - Immunology 2020Quote: ... The pseudo-particle-containing supernatant was then concentrated to 5% of its original volume using an Amicon Ultra with a 100 KDa cutoff at 16 °C (Merck Millipore).
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bioRxiv - Neuroscience 2020Quote: ... Tissue was incubated overnight at 4°C in either monoclonal or polyclonal antisera (for anti-GABA: Sigma #A2052, dilution 1:5000; for anti-PV: Sigma #P3088 ...
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bioRxiv - Immunology 2020Quote: ... so the amount needed for a single day was dissolved in EtOH with heating to 37°C and then diluted in corn oil (Sigma) such that 100ul had 5mg ...
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bioRxiv - Immunology 2020Quote: ... IELs were plated in 48-well plates and incubated at 37°C with 100ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma) and 200ng/mL ionomycin (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... L4 DRG and L4 dorsal and ventral roots were dissected from PFA-fixed mice and post-fixed in PFA/glutaraldehyde buffer at 4°C (20 ml 0.9% saline, 10 ml 10% PFA, 10 ml 10% glutaraldehyde (Sigma-Aldrich), 20% dextran (20,000 MW ...