Labshake search
Citations for Millipore Sigma :
4101 - 4150 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Cells were then incubated overnight at 4°C with PBS-T containing 2% NGS and antisera against Olig-2 (1:500,Millipore) A2B5 (which stains for OPCs ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, #MRCPRT010 Ultracel YM-10). The mixture was centrifuged at 1000 rcf for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... sections were fixed with ice-cold 100 % acetone for 10 min at −20 °C and washed with PBS-T (0.1 % v/v Tween 20, Sigma-Aldrich) before blocking with 5 % BSA in PBS (m/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... of FA was calculated by centrifuging the prepared nanoparticles at 12,000×g at 4 °C for 45 min (Sigma, Germany). The concentration of unencapsulated FA was analyzed by HPLC (Agilent Technologies Model 1260 ...
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bioRxiv - Plant Biology 2023Quote: ... 1.4 ml of the remaining filtered total protein extract was mixed with 30 μl of anti-c-Myc agarose beads (A7470, SIGMA) and incubated end over end for 90 minutes at 4 ºC ...
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bioRxiv - Immunology 2022Quote: ... the membrane slide was stored in its carrier box and in dry ice until final storage at -80°C prior to staining with Cresyl violet acetate (SIGMA). Preparation of Cresyl violet acetate is required one day prior to staining the mounted tissues ...
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bioRxiv - Immunology 2022Quote: ... cell suspensions were stimulated 4 h at 37°C with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) (Sigma), 1 μg/mL ionomycin (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... the cells were firstly cultured for 4 h in a 37°C/5% CO2 incubator with 50 ng/ml PMA (Sigma) and 1 mM ionomycin (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... Free-floating sections were incubated at 4°C for 16 hours with rabbit anti-Myc (1:500, C3956; Sigma-Aldrich) primary antibody in PBS/0.5% normal donkey serum/0.1% Triton X-100 ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were gently minced and digested at 37[°C for 25[minutes with an enzyme cocktail (5 mg collagenase type I with 2.5 mg trypsin, Sigma-Aldrich) in 5 ml DMEM ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei and other debris were pelleted at 2,000 g for 5 min at 4°C and the supernatant was filtered through three layers of 100 μm pore nylon mesh (Millipore), and a 5 μm pore PVDF syringe filter (Millipore) ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads-antibody-protein mix was incubated overnight at 4°C and bounds protein were eluted with 35 μg HA peptides diluted in PBS (Sigma); then Laemmli buffer was added and the eluate heated at 95°C 2 min.
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bioRxiv - Molecular Biology 2022Quote: ... Elutions were performed by incubating the beads overnight at 4° C with 800 elution buffer and 200 ul of 5mg/ml flag peptide (Sigma). The buffer exchange (into elution buffer ...
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bioRxiv - Neuroscience 2022Quote: ... with PBS pH 7.4 for 10 min at 4400 x g and 4°C using an Amicon Ultra-4 concentrator with 3 kDa cutoff (Merck Millipore). The degree of labelling (DOL = 1.96 ...
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bioRxiv - Neuroscience 2022Quote: ... Unencapsulated molecules were removed by washing at least 4 times the LPs suspensions with PBS pH 7.4 for 20 min at 4°C and 5000 x g using an Amicon Ultra-4 concentrator with 100 kDa cutoff (Merck Millipore). The degree of encapsulation was calculated by subtracting from the total mass of the molecule to be encapsulated that present in the flow-through and was found to be higher than 75% in all LP preparations.
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bioRxiv - Microbiology 2022Quote: ... Samples were heated at 100°C for 5 minutes and then separated by SDS/PAGE and transferred to PVDF membranes (Millipore). Membranes were then probed with primary antibodies specific for murine caspase-11 (#C1354 ...
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bioRxiv - Immunology 2022Quote: ... DLN cells were counted using magnetic beads and 1 × 106 total DLN cells were incubated 48 hours at 37°C in RPMI 1640 (R8758, Sigma) containing 2% FBS and 0.1% Penicillin-Streptomycin (P4333 ...
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bioRxiv - Plant Biology 2022Quote: ... Samples which were used to create sRNA sequencing libraries were flash frozen in liquid nitrogen and stored at −80°C until total RNA extraction was performed using a BioSpec Mini-Beadbeater and Tri-Reagent (Sigma) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: ... was added to a final concentration of 1% and incubated for 5 minutes at 37°C shaking at 800 rpm and the reaction quenched with 60µl β-mercaptoethanol (Sigma). DMS modified RNA was purified using RNA Clean and Concentrator-5 kit and eluted in 10 ul water ...
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bioRxiv - Bioengineering 2022Quote: ... and antigen retrieval at 98°C for 15 minutes (KOS microwave tissue processor, Milestone) in citrate buffer pH6.0 (Sigma, C999). Prior to staining ...
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bioRxiv - Cell Biology 2022Quote: ... All samples with SDS sample buffer were heated at 95°C for 5 minutes and reduced with 5% 2-mercaptoethanol (Sigma) as needed.
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bioRxiv - Cell Biology 2022Quote: ... The tissues were treated for 20-40 min at +4⁰C with 1,25U/ml of Dispase II (4942078001; Sigma Aldrich) in PBS and 3-4 min at room temperature with a pancreatin-trypsin mixture (2.5mg/ml pancreatin [P3292 ...
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bioRxiv - Immunology 2022Quote: ... T cell subsets were rested overnight at 37°C then stimulated for 6 h with phorbol 12-myristate 13-acetate (25 ng/mL; Sigma) and ionomycin (1 mg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... An equivalent of 1 μl of the original IVT was used for TCA precipitation and filtered onto a GF/C membrane (Millipore). The cap-analogue beads were washed 5 times with binding buffer and the final bead fraction suspended in SDS-PAGE sample buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from the HEK293 parent cell line and the homozygous c.1625A>G knock-in CRISPR/Cas9 clones C2 and F4 using TriPure (Sigma). cDNA was synthesized using POLR3B exon 18-specific primer 5’-TGTTGTGTTCGTACAGTGCAATGT and the Superscript III First-strand synthesis system (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... washed three times with 200 μl of PBS and centrifuged at 1811 g in a pre-cooled centrifuge at 10°C prior to fixation in 100 μL 4% PFA (Sigma) for 45 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated (1 h, 50°C) with 2 µL 10% SDS and 5 µL proteinase K (10 mg/mL, Sigma). Chromatin was recovered by phenol/chloroform extraction and resuspended in 30 µL TE (1 mM tris-HCl pH8.0 ...
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bioRxiv - Cell Biology 2024Quote: ... The clarified lysate was incubated on a rotator (50 min, 4°C) with 1 mL (packed volume) HIS-Select HF Nickel Affinity Gel (Sigma). The beads were collected in 15 mL Poly-Prep chromatography column (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... the appropriate dilutions of the fermentation medium were incubated for 30 min at 37 °C in the presence of 5 mM sodium phytate (Sigma‒Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... cells were harvested by centrifugation (15 min; 4,000 g; 4°C) and pellets were lysed with BugBuster® ready mix (Millipore) for 25 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... the distilled (DI) water in the devices was replaced by Poly-L-Ornithine solution (PLO) (Sigma-Aldrich, A-004-C) at a concentration of 0.1 mg/mL and incubated at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... in PBS solution for 5 min and permeabilized in pre-warmed (to 37° C) 0.2% Triton X-100 (Sigma, T8787) in PBS for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were reconstituted in 100 µL and 2 µL of the sample was injected from a 4 °C autosampler into a ZIC-pHILIC 150 × 2.1 mm 5 µm particle size column (EMD Millipore) with a ZIC-pHILIC 20 x 2.1 guard column in a Vanquish Duo UHPLC System (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: C2C12 cells were cultured at standard incubation conditions (37 °C, 5% CO2, 95% humidity) in Dulbecco’s modified Eagle medium supplemented with 15% fetal bovine serum (Sigma-Aldrich), 2% penicillin-streptomycin (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cre-mediated recombination was induced by intraperitoneal injections of 5 doses of poly(I) poly(C) (pIpC) (400 ug per dose; Sigma) administered every other day for 10 days to 6- to 12-week-old mice ...
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bioRxiv - Molecular Biology 2024Quote: ... The gels were run at 150 V for approximately two hours at 4 °C and transferred onto 0.2 µm PVDF membranes (Millipore IPVH00010) using the Mini Trans-Blot cell system (BioRad 1703930 ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were post-fixed in the same solution for 12 h at 4°C and then cryoprotected for at least 24 h in 30% sucrose (Sigma) in 1× PBS at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... sections were subjected to a blocking step with 5% NDS and then incubated overnight at 4 °C with goat anti-Chat (at a dilution of 1:250; #AB144P, Sigma) and Streptavidin-AF488 (at a dilution of 1:250 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight with agitation at 4°C with mouse anti-Flag (Sigma-Aldrich Cat# F1404, RRID: AB_262044) diluted in 2% PBST and 2% NGS ...
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bioRxiv - Immunology 2024Quote: ... MLN cells were collected from Balb/c mice and maintained in RPMI 1640 medium (#R8758, Sigma-Aldrich, St. Louis, USA) supplemented with 10% FBS ...
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bioRxiv - Physiology 2024Quote: ... and maintained at 37 °C with 5% CO2 in a 1:1 mixture of DMEM and Ham’s F-12 medium (D 8900, Sigma-Aldrich), supplemented with 50 ng/ml mouse Nerve Growth Factor-7S (NGF ...
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bioRxiv - Genomics 2024Quote: ... Embryos were then incubated overnight at 37°C under 5% CO2 in humidified air in 4-well culture dishes containing KSOM media (Millipore). Experiments were approved by the SIMR IACUC and were performed following the committees’ guiding principles.
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bioRxiv - Plant Biology 2024Quote: ... and subjected to immunoblot analysis with the relevant primary and secondary antibodies (anti HA rabbit polyclonal, Abcam ab20084; anti AV2 CP rabbit polyclonal, DSMZ RT-0953-0645/1; anti c-Myc mouse monoclonal, Sigma M4439 ...
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bioRxiv - Neuroscience 2024Quote: ... dissociated with forceps and digested at 37°C for 30 minutes via the addition of digestion solution (6 units of Papain (Sigma) and 9 units of DNase I (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... Media was incubated at 4 °C for 2 hours in 20% PEG solution (40% w/v stock: 2.5 M NaCl, 40% PEG (Sigma P2139) followed by a 20 min 3,000 x g spin at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... Tissues were fixed in 4% paraformaldehyde for 24 hours at 4 °C and washed with Dulbecco’s phosphate-buffered saline (DPBS, Sigma, USA). After dehydrated through a graded ethanol series to absolute ethanol ...
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bioRxiv - Plant Biology 2024Quote: Total RNAs were extracted from untreated (0) and UV-C treated WT and jmj27 plants (time points 30 min, 1h and 2h) using Tri-Reagent (Sigma). Reverse transcription reaction (RT ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated in appropriate secondaries diluted in 2% bovine serum in PBS for 1 h at 20-25°C and counterstained with Hoechst 33257 (1:1000; Sigma). For brain tissue incubated in anti-INO80 antibody (ab118787 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues derived from at least two mice were pooled and digested for 30 minutes at 37°C with Earl’s Balanced Salt Solution (EBSS) (#E2888, Sigma-Aldrich) containing 200 mg/l L-Cysteine (#C7352 ...
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bioRxiv - Cell Biology 2024Quote: ... Eluates were dialysed back into Buffer A overnight at 4 °C prior to concentration with 10 kDa MWCO Amicon centrifugal filters (Millipore, Sigma), quantification by absorbance at 280 nm ...