Labshake search
Citations for Millipore Sigma :
4151 - 4200 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... PLA signals were amplified by incubating cells with DNA polymerase for 100 min at 37°C (DUOLINK in Situ detection reagent red, DUO92008, Sigma). Confocal images were acquired using a Leica laser scanning confocal microscope and analyzed via Fiji software as previously described (19).
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bioRxiv - Developmental Biology 2024Quote: ... we adapted a protocol that uses Accutase and Papain as dissociation agents.93 The cells were first rinsed with PBS and then incubated at 37°C for 25 minutes in a dissociation buffer composed of 50% Accutase (Sigma) and 10% Papain (Sigma ...
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bioRxiv - Immunology 2024Quote: ... Tissue was minced and incubated for 15 min at 37°C with collagenase type IA (Sigma Aldrich; St. Louis, MO) in PBS with 0.5% BSA and 2mM EDTA Following red blood cell lysis ...
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bioRxiv - Developmental Biology 2024Quote: ESCs were seeded onto fibronectin-coated 12-well plates (16.7μg/ml fibronectin [Millipore; FC010] at 37°C for 1 hour) at a density of 1×105 cells/well ...
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bioRxiv - Immunology 2024Quote: ... The pellet was collected and resuspended in 500 μL of Reagent C supplemented with Protease Inhibitor cocktails (P8340, Sigma Aldrich). Following separation ...
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bioRxiv - Immunology 2024Quote: ... the transfected cells were harvested by centrifugation (7,000 RPM for 60 minutes at 4°C) and the supernatant was cleared by vacuum filtration (0.45 µm filtration units, Millipore Sigma). The expressed SOSIPs were purified as described previously (13 ...
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bioRxiv - Microbiology 2024Quote: ... Cell cultures were maintained at 37°C with 5.0% CO2 and sub-cultured by washing with PBS (Sigma, D5652-10X1L) followed by detachment using 0.05% trypsin + 0.53 mM EDTA (Corning ...
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bioRxiv - Microbiology 2024Quote: ... the hydrolysis of formamide and acrylamide after 30 min at 30 °C by the addition of 100 µl phenol-nitroprusside (Sigma). For color development 50 µl alkaline hypochlorite (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... Cultures were induced by addition of DNA-damaging antibiotics to a final concentration of 3 μg/ml: mitomycin C (Sigma), norfloxacin (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... This committed parasites were then split into two flasks and treated for 4 hours at 37°C with either 100nM rapamycin (Sigma) or dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2024Quote: ... 10 ng recombinant IdeS was pre-incubated with sera dilutions as indicated at 37°C 1 h before addition of 100 ng human IgG (Sigma) in assay diluent (AD ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were transiently transfected with Cx26 constructs tagged at the C-terminus with a fluorescent marker (mCherry) according to the GeneJuice Transfection Reagent protocol (Merck Millipore).
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bioRxiv - Cell Biology 2023Quote: Livers of anesthetized mice were perfused by inserting a 22-gauge syringe into the portal vein and delivering 25 ml of pre-warmed (37 °C) perfusion buffer [Krebs-Henseleit buffer (Sigma) with 0.5 μM EDTA] ...
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bioRxiv - Cancer Biology 2023Quote: ... Parental and genetically modified MDA-MB 231 cells were cultured at 37 °C and 0 % CO2 in Leibovitz’s L-15 medium (Sigma-Aldrich) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were treated with 1 ml pre-cooled (-20°C) mixture of methyl tert-butyl ether (MTBE, Sigma 306975), Optima LC-MS grade methanol (A456-212 ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were grown at 30 °C in minimal synthetic media or agar plates containing 6.8 g/L yeast nitrogen base without amino acids (Sigma-Aldrich), 2 g/L dextrose (Formedium) ...
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bioRxiv - Cell Biology 2023Quote: ... were fused with mNeongreen at their C-terminus with a flexible peptide linker and cloned into pET28a vector (Millipore; 69864). Protein expression in Escherichia coli BL21(DE3 ...
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bioRxiv - Cell Biology 2023Quote: ... The aqueous layer was transferred to a new tube and then mixed 1:1 with pre-chilled (-20°C) isopropanol (100%, Sigma) and incubated at -20°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... All samples were then incubated at 37°C for 30 min before phenylmethanesulfonyl fluoride (PMSF, 5 mM final concentration, Sigma) was added and incubated for 10 min at room temperature to terminate proteinase digestion ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged recombinant proteins of HSF5 N-terminal region (aa1-209) and HSF5 C-terminal region (aa324-624) were produced by inserting cDNA fragments in-frame with pET19b (Novagen) respectively in E ...
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bioRxiv - Genetics 2023Quote: ... for one hour in a 37°C tissue culture incubator followed by 10 µg/mL Laminin (L2020-1MG, Sigma-Aldrich) coating in the same way ...
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bioRxiv - Developmental Biology 2023Quote: ... the transduced cells were transferred onto murine embryonic fibroblast (MEF)-coated plates and cultured for one month at 37 °C and 5% CO2 in a stem cell medium containing DMEM/Ham’s F-12 (cat. No. 1445C, Sigma Aldrich), 20% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was clarified by centrifugation at 20,000 × g for 5 min at 4°C and filtered on an Ultrafree-MC GV Centrifugal filter (Millipore, UFC30GV0S). Superose 6 Increase HR 10/300 column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... Polyacrylamide gels were then immediatly coupled to either human plasma fibronectin (1 mg/mL overnight at 37◦C; FC010, Millipore) or ICAM-1 (0.1 mg/mL Recombinant Protein A (Novex ...
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bioRxiv - Cell Biology 2023Quote: ... Myc3 fragment was obtained from ATF6α(C)-TAP2 (Myc3-TEV-ProteinA) (George et al., 2021) and inserted into A p3xFlag-CMV-14 expression vector (Sigma) at the site of BamHI to construct p3xMyc-CMV-14 expression vector ...
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bioRxiv - Immunology 2023Quote: CeMbio cultures were grown under aerobic conditions at 25 °C with 170-200 RPM shaking in TSB (Sigma-Aldrich T8907) containing 50 µg ml-1 ampicillin for 20-24 h ...
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bioRxiv - Bioengineering 2024Quote: ... hard baked at 95°C for 5 to 10 min and developed in propylene glycol monomethyl ether acetate (Sigma Aldrich). For the microfluidic devices ...
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bioRxiv - Evolutionary Biology 2022Quote: ... LC-ESI-MS analysis was carried out at 35 °C by loading 1 μg of the sample in 0.1% formic acid (Sigma-Aldrich) onto a 50-cm μPAC C18 column (Pharma Fluidics ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1 mM EDTA overnight at 4 °C Protein prep was then concentrated with Amicon Ultra-15 Centrifugal Filter Units 3000K (Millipore) to a final concentration of 1.6 mg/ml measured by Bradford assay (Thermo).
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bioRxiv - Cell Biology 2023Quote: ... and treated for 4 h at 37°C in a polymerase solution containing fluorescent nucleotides as described (Sigma- Aldrich, Duolink). Samples were then washed 2x with buffer B (Sigma-Aldrich ...
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bioRxiv - Physiology 2023Quote: ... Jejunum tissue was then digested at 37°C for approximately 15 min in Thermoshake (Gerhardt) with 0.6 mg/ml type VIII collagenase (Sigma-Aldrich). Colonic tissue was digested at 37°C for approximately 25 min with 0.85 mg/ml type V collagenase (Sigma-Aldrich) ...
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bioRxiv - Physiology 2023Quote: ... Colonic tissue was digested at 37°C for approximately 25 min with 0.85 mg/ml type V collagenase (Sigma-Aldrich), 1.25 mg/ml collagenase D (Sigma- Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were fixed 72 h after transfection in -20°C methanol and subjected to PLA using the Duolink In Situ Red Starter Kit Mouse/Rabbit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... the zona was removed by bathing the oocytes or embryos in acidic Tyrode solution prewarmed at 30 °C (Sigma, T1788). The following primary antibodies were applied to the specimens for 2 h at RT ...
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bioRxiv - Biochemistry 2023Quote: ... and the supernatant was filtered at 300 × g for 3 minutes at 4°C through a 0.45-μm 96-well filter plate (Millipore, MSHVN4550) to remove protein aggregates ...
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bioRxiv - Biochemistry 2023Quote: All peptides used in this study probes were designed as 11-mers bearing biotinylated PEG2 linker in the N terminus and an amide group in the C terminus and were synthesized using Fmoc-solid-phase peptide chemistry with Novasyn TGR resin (Merck Millipore) as described in the Novabiochem Innovations 01/11 ...
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bioRxiv - Immunology 2022Quote: ... RT complete IntestiCult containing 25μg/ml was added, and enteroids were incubated (37°C, 5% CO2) until fixation with 4% paraformaldehyde (PFA; Sigma Aldrich) at 4h p.i ...
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bioRxiv - Microbiology 2023Quote: ... and remaining lysate was end-over-end incubated with 5 µg of primary Ab overnight at 4°C [Rabbit-anti-HA (Sigma), Rabbit-anti-Flag (Sigma) ...
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bioRxiv - Microbiology 2022Quote: ... Biological ammonia oxidation was suppressed by overnight incubation at 50 °C with 0.05 g penicillin g L-1 (Sigma Aldrich) (H ...
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bioRxiv - Immunology 2023Quote: ... C.Striatum and C.Diphtheriae were incubated at 37°C in a shaking incubator in 611 PGT media containing 0.5mM 2,2’-bipyridyl (Sigma-Aldrich, D2163050). Growth was closely monitored by measuring OD600 every hour for 4 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... and the derivative line (Ring1B− /−) (Illingworth et al. 2015) were cultured at 37°C on 0.1% gelatin-coated (Sigma G1890) Corning flasks in GMEM BHK-21 (Gibco 21710-025 ...
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Doxorubicin inhibits phosphatidylserine decarboxylase and confers broad-spectrum antifungal activitybioRxiv - Pathology 2023Quote: ... mycelial samples were freeze-dried and incubated in hot isopropanol (75°C) containing 0.05% butylated hydroxytoluene (BHT) (Sigma-Aldrich, Germany) for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... cells were fixed overnight in 4% paraformaldehyde in PBS at 4°C and plated onto 0.1% poly-L-lysine (Sigma-Aldrich) coated cell slide ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein lysates were denatured at 95°C for 5mins before being loaded into a TruPAGE 4-20% precast gradient gel (Sigma) and undergoing SDS-PAGE at 120 V for 2 hours ...
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bioRxiv - Physiology 2023Quote: ... Splenocytes were incubated for 1 hour at 37°C in 96-well plates in RPM11640 medium (3 ml) (Sigma-Aldrich) with or without EPO (35U/well ...
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bioRxiv - Neuroscience 2023Quote: ... 10% goat serum in 0.1 M PBS) at 37 °C overnight and then incubate it with propidium iodide (1:1000, Sigma, P4864) or antibodies against SARS-CoV-2 (COVID-19 ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were extracted and swiftly placed in cooled (4 °C) dissection media consisting of Earle’s balanced salt solution (Sigma-Aldrich); 6.1 g/L HEPES (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were spun at 30,000 g for 10 min at 4°C and the cleared lysate was incubated with 40 µl anti-FLAG agarose bead slurry (Sigma) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The pre-cleared chromatin was then incubated overnight at 4°C with 10 µL of either rabbit IgG (1 mg/mL, IGBMC facility) or rabbit anti-CTCF (07-729, Millipore). Immunoprecipitated chromatin was then adsorbed to 100 µL prepared protein A/protein G-Dynabeads for 3.5 h at 4°C on a rotating wheel ...
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bioRxiv - Neuroscience 2023Quote: ... were cultured at 37° C and 95%O2/5%CO2 in Dulbecco’s modified Eagle/F-12 medium (DMEM/F-12, Sigma Aldrich), supplemented with 10% foetal calf serum ...