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Citations for Millipore Sigma :
3701 - 3750 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Synechocystis PCC6803 (glucose tolerant, obtained from K. Hellingwerf, UvA, Amsterdam) cells were maintained at 30°C on BG-11 media (Sigma) supplemented with 10 mM TES-NaOH (pH 8.2) ...
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bioRxiv - Plant Biology 2019Quote: ... Fixed plant material was digested for 4.5 hours at 37° C in an enzyme mixture consisting of 4 % (w/v) cellulase Onozuka R10 (Sigma 16419) and 20 % (v/v ...
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bioRxiv - Cell Biology 2019Quote: ... for 30 minutes and incubated overnight at 4°C with anti-TAT1 (1:250) and/or anti-GFP (1:500, Sigma) antibodies ...
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bioRxiv - Immunology 2019Quote: ... by spinoculation in 96-wells plate (1,200 x g, 90 min at 16°C) with polybrene (4 μg/ml, EMD Millipore). 6h after infection / transduction ...
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bioRxiv - Cancer Biology 2019Quote: ... each sample was reduced with DTT (final concentration of 20 mM) at 57°C for 1 hour and loaded onto a MicroCon 30-kDa centrifugal filter unit (Millipore) pre-equilibrated with 200 μl of FASP buffer [8 M urea and 0.1 M tris-HCl (pH 7.8)] ...
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bioRxiv - Immunology 2019Quote: ... Residual tissue was then washed for 8 min in 15 ml of IMDM containing 10% FCS at 37°C before being minced and digested in 15 ml of IMDM containing 1 mg/ml collagenase type VIII (Sigma) and 10 U/ml DNase I (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... reactions were incubated at 37 °C for 5 min and stopped by the addition of malachite green reagent (Sigma Aldrich). Absorbance was measured at 620 nm on a FLUOstar Omega plate reader (BMG).
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion was performed overnight at 37 °Cwith Lys-C (Wako) at an enzyme/protein ratio 1:75 and trypsin (Sigma) at an enzyme/protein ration of 1:50 ...
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bioRxiv - Microbiology 2019Quote: ... J774 murine macrophage-like cells were cultured for a minimum of 4 passages in T75 tissue culture flasks at 37°C 5% CO2 in DMEM (High glucose, Sigma) supplemented with 10% Fetal Bovine Calf Serum (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples were reverse cross-linked overnight at 65°C (under agitation-900 rpm) in presence of 100 μl of 20mg/ml Proteinase K (P2308, Sigma) and 60 μL 20% SDS ...
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bioRxiv - Molecular Biology 2019Quote: ... Mouse CTCF cDNA corresponding to the full-length 736 amino acids was cloned with C-terminal 6xHis tag into pFLAG-2 (Sigma). FLAG-CTCF-6xHis protein was induced in Rosseta-Gami B-cells (EMD Millipore ...
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bioRxiv - Developmental Biology 2019Quote: Embryos were fixed at stage 10.75 to 12 in MEMFA for 2 hours at room temperature or Dent’s fixative 6 for 6 hours at −20°C (rehydrate with gradual change of methanol/PBS) and embedded in fish gelatin (16.7ml of 40% gelatin (Sigma-Aldrich) and 7.5g sucrose in 50ml ddH2O ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... or α/β-tubulin (1:1 mixture) (1:100 in PBS; 2 hr at 37°C) (Sigma; T9026, T4026, USA). FITC-conjugated anti-mouse IgG (Jackson ImmunoResearch ...
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bioRxiv - Cell Biology 2019Quote: ... 10% of the cleared lysate was kept as input and the rest incubated for 2h on a rotating wheel at 4°C with anti-FLAG M2 magnetic beads (Sigma). Beads were subsequently washed three times with lysis buffer and immunoprecipitated proteins eluted with FLAG peptide.
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bioRxiv - Biophysics 2019Quote: ... Tag cleavage was carried out overnight at 4 °C on a roller with 10 units of HRV 3C protease (Novagen/Merck chemicals Boulevard Industrial Park ...
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bioRxiv - Microbiology 2019Quote: ... were fixed for 15 min at 4° C in the dark with 25% electron microscopy (EM) grade glutaraldehyde (0.5% final concentration; Sigma-Aldrich), flash-frozen in liquid nitrogen and stored at −80° C until analysis.
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bioRxiv - Developmental Biology 2019Quote: Tissues were isolated and immediately frozen in liquid nitrogen before storing in −80°C or put in TRI Reagent (Sigma) for RNA extraction immediately ...
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bioRxiv - Microbiology 2019Quote: ... The cultures were incubated for an additional 4 h at 37°C and the media was replaced with microscopy-grade media (Sigma DMEM without Phenol Red ...
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bioRxiv - Biophysics 2019Quote: ... Live CH27 cells in suspension were pelleted at 500g for 5 minutes at 4°C then incubated on ice for 10 minutes with 3.5μg/mL biotinylated transfer-rin (Sigma-Aldrich: T3915) in the live cell compatible buffer ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins (LuxR was tagged N-terminally and H-NS was tagged C-terminally) were captured from clarified lysates using anti-FLAG agarose M2 beads (Sigma). Lysates were incubated with the anti-FLAG resin for 90 minutes at room temperature in immunoprecipitation buffer (50 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow was isolated from the femurs and tibia of mice and grown at 37°C with 5%CO2 in bacterial plastic dishes in alphaMEM with 15% FBS (Sigma), 100U/mL penicillin ...
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bioRxiv - Plant Biology 2019Quote: ... Pseudomonas syringae strains were grown at 28°C (with shaking at 200 rpm) in King’s B liquid medium (Sigma-Aldrich) containing appropriate antibiotics (King et al. ...
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bioRxiv - Biochemistry 2019Quote: ... The resulting supernatants were incubated overnight at 4°C with 0.5-1 ml of swelled glutathione-agarose resin (Sigma G4510), after which the slurries were loaded onto a column ...
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bioRxiv - Genomics 2021Quote: ... as well as two monoclonal anitbodies targeting the KMT2A C-terminus: Mouse anti-KMT2A (1:100, Millipore Cat #05-765) refered to as KMT2A-C1 ...
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bioRxiv - Molecular Biology 2021Quote: ... After incubation for 15 min at 37 °C the amount of released phosphate was estimated with Malachite Green Phosphate Assay (Sigma), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... the samples were dissolved in 50 μL of 20 mg/ml methoxyamine hydrochloride in pyridine for 1.5 h at 33°C followed by derivatization with N-Methyl-N-(trymethylsolyl)trifluoroacetamide (MSTFA, Sigma Aldrich) for 2 h at 35°C.
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bioRxiv - Cancer Biology 2021Quote: ... Then the cells were cultured in 10% FBS RPMI-1640 medium with 1 μg/ml mitomycin C (Sigma, cat. M4287) to prevent cell division ...
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bioRxiv - Biophysics 2021Quote: ... Larvae were raised in the dark at 25°C for 4 days on fly food containing trans-retinal (Sigma, R2500), at a concentration of 200 µM ...
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bioRxiv - Cancer Biology 2020Quote: ... Pellets containing 3-5 million cells were prepared and frozen at −80°C in albumin/DMSO (90/10, Sigma-Aldrich). Note that the wild-type for both BRAF and NRAS (WT ...
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bioRxiv - Developmental Biology 2021Quote: ... This was followed by ∼18 hours digestion at 37°C in 0.25 mg/mL of Collagenase Type 1S (Sigma Aldrich) in growth media ...
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bioRxiv - Developmental Biology 2021Quote: ... E13.5 embryonic hearts were isolated and cultured in DMEM (with 10% BSA) containing DMSO or 10uM Compound C (Millipore, #171620) or DMSO for 6 hours in a cell culture incubator (37 °C ...
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bioRxiv - Immunology 2020Quote: ... two STAT-3 inhibitors (STAT-3 inhibitor III (WP-1066) and STAT-3 inhibitor XIII (C-188-9) were tested (Merck, Sigma) and WP-1066 was used for infection studies at a final concentration of 12 μM ...
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bioRxiv - Genomics 2020Quote: ... were then applied at 37°C according to the manufacturer’s instructions and slides were mounted in Duolink mounting medium with DAPI (Sigma Aldrich). Slides were visualised as for immunocytochemistry ...
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bioRxiv - Immunology 2021Quote: ... the following components were added: 2 mM L-Glutamine (Fisher), 10% heat-inactivated (56°C, 60 min) human AB serum (Sigma), 12.5 mM HEPES (Fisher) ...
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bioRxiv - Plant Biology 2020Quote: ... RNA was precipitated overnight at 4°C in 100% ethanol containing 115 mM Na-Ac (pH 5.2; Sigma Aldrich, Germany) to further clean up and increase RNA yield ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were divided in 3 tubes and incubated overnight at 4°C in a rotating wheel with 2.5μg per tube of the anti-H3K4me3 (Sigma, 07-473), anti-H3K27me3 (Abcam ...
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bioRxiv - Systems Biology 2020Quote: ... Seq-Well arrays were sealed in a dry 37°C oven for 40 minutes before being submerged in a lysis buffer containing guanidium thiocyanate (Sigma), 1mM EDTA ...
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bioRxiv - Cancer Biology 2020Quote: ... were dissolved in DMSO and stored at −20°C. Bafilomycin A1 (Cat. No. SML1661) and Chloroquine diphosphate salt (Cat. No.C6628) were obtained from Sigma-Aldrich. Chloroquine was dissolved in water and stored at −20°C.
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bioRxiv - Molecular Biology 2020Quote: ... 4°C) twice with 300 μL of Elution buffer (Lysis buffer A with 1 mg/mL 3 × FLAG peptide (Sigma)) ...
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bioRxiv - Bioengineering 2021Quote: ... Samples for DNA quantification were incubated overnight at 56 °C in Proteinase K solution (1mg/ml Proteinase K (Sigma-Aldrich) in Tris/EDTA buffer ...
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bioRxiv - Bioengineering 2021Quote: ... lithium phenyl-2,4,6-trimethylbenzoylphosphinate in PBS at 50°C and an oil solution of mineral oil solution containing 0.25%(v/v) concentration of Span 80 (Sigma Aldrich) converge in the flow-focusing device (Figure 1A ...
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bioRxiv - Bioengineering 2021Quote: ... Zeiss) slides and were either stored at −80 °C in 50 ml falcon tubes filled with molecular sieves (Sigma-Aldrich) or processed further for serial dehydration with ethanol and air dried for 15 min under the hood ...
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bioRxiv - Biochemistry 2020Quote: ... The recovery of RNase A activity was measured by monitoring the linear increase in absorbance at 295 nm on a U-3900 spectrophotometer (HITACHI) at 30°C after addition of 1.25 mM cytidine 2′,3′-cyclic monophosphate monosodium salt (cCMP, Sigma-Aldrich) to reaction mixtures at indicated time points.
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bioRxiv - Biochemistry 2020Quote: ... The recombinant proteins were overexpressed in E.coli BL21 (DE3) at 17 °C overnight and then purified by Glutathione-agarose (Sigma #G4510). Briefly ...
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bioRxiv - Biochemistry 2020Quote: ... whereas the C-terminal flanking sequence is removed by the treatment with HRV-3C protease (Millipore Sigma, St. Louis, MO). For crystallization ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated overnight at 4°C with guinea pig anti-vGlut2 antiserum (1:12,000, cat# AB2251-1, Millipore Sigma) in PBS-T containing 2% normal donkey serum ...
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bioRxiv - Immunology 2020Quote: ... 2020) and c) PMA/Ionomycin in the presence of Golgi transport inhibitors - 10 μg/mL of Brefeldin A (Sigma-Aldrich). After stimulation ...
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bioRxiv - Neuroscience 2021Quote: ... The lysates were cleared by centrifuging at 2000 xg for 10 min at 4°C and treated with DHPC (to 30 mM, Avanti) and NP-40 (to 1%, Ipgal-ca630, Sigma) for 5 min in ice ...
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bioRxiv - Neuroscience 2021Quote: ... resuspended in PBS for 15 minutes and then incubated 1h at 37°C with 60 µg/ml of PI (Sigma). Cells were collected by centrifuge and resuspended in ice-cold PBS for FACS analysis.
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bioRxiv - Neuroscience 2021Quote: ... The brains were cut into 30 μm sections with a cryostat (Hyrax C 25, Zeiss) and collected in PBS with 0.1 % Triton A-100 (Sigma-Aldrich), PBST.