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Citations for Millipore Sigma :
3751 - 3800 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated at 37°C for 30 minutes prior to fixation with 4% PFA (Electron Microscopy Services) containing 4% sucrose (Sigma) for 30 min at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... The virus-containing supernatant was concentrated by ultracentrifugation in a SW-28 rotor (25,000 rpm, 2 h, 4°C) using OptiPrep density gradient medium (Sigma-Aldrich). Virus containing fractions were collected ...
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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumorsbioRxiv - Cancer Biology 2020Quote: ... and incubated at 37° C for 90 minutes followed by addition of 20μL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert–Butyldimethylchlorosilane (Sigma 375934) and incubated at 60°C for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... After sonication (Bioruptor, 10 min, cycle 30/30 sec) and centrifugation (4°C, 15 min) anti-FLAG agarose beads (M2, Sigma) were added to the supernatant and incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at room temperature or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Cell Biology 2021Quote: ... for 15 min and blocked for 2 h at RT or overnight at 4°C using 2% BSA (Bovine Serum Albumin, Sigma). Samples were incubated with respective primary antibody (Ab ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysates were incubated for 2 hours at 4 °C with 30 µl of precleared slurry EZ view Red Anti-FLAG M2 Affinity Gel beads (Sigma). Beads were washed three times with RIPA buffer and resuspended in 50 µl of Laemmli buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Polar metabolites were first methoximated with methoxyamine hydrochloride in pyridine (Sigma, 20 μl, 30 mg/ml, 2 hours, 37 °C) and then derivatized with TMS reagent (N,O-bis(trimethylsilyl ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated at 37°C for 90 minutes followed by addition of 20µL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert– Butyldimethylchlorosilane (Sigma 375934) and incubated at 60°C for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... Frozen tissue or cell pellets were boiled for 24 hours at 100°C in 500µL (cell pellets) −1mL (tissue) 6M HCl for amino acid analysis (Sigma 84429). 50µL (tissue ...
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bioRxiv - Neuroscience 2020Quote: ... The diluted AAVs were then desalted and concentrated by centrifugation at 5000 rpm for 30 minutes at 20°C in a pre-rinsed Amicon Ultra-15 filter (Millipore) in 1X PBS-MK ...
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bioRxiv - Neuroscience 2020Quote: ... These were formed by 24h incubation at 37°C of a 1mg/ml solution of Aβ 1-42 peptide (Sigma) in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... cortex and hippocampal slices (300 µm thick) were kept at 4°C in Krebs-HCO3 buffer during the dissection and transferred into a Millicell Insert (Millipore).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and antigen retrieval was performed in 37 °C trypsin (pH 7.8) for 1 h or 10 mM sodium citrate buffer (pH 6) (Sigma-Aldrich) at 95 °C for 10 min ...
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bioRxiv - Physiology 2021Quote: ... were incubated in Krebs buffer (1.5 mls, 37°C, 5% CO2) containing the angiotensin converting enzyme inhibitor enalapril (20 μM; Catalog# PHR1289, Sigma-Aldrich) to eliminate further conversion of angiotensin I to AngII ...
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bioRxiv - Plant Biology 2021Quote: ... Substrate specificity of recombinant AtPME2 activity was determined at pH 7.5 and 28°C using commercial citrus pectin (Sigma-Aldrich, DM >85% ...
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bioRxiv - Plant Biology 2020Quote: ... The soluble fraction was collected after centrifugation at 267.000 rcf at 4°C for 25 min and concentrated using Amicon Ultra centrifugation units with a 30 kDa cutoff (Millipore). Protein concentrations were measured colorimetrically by Bradford assay (Bradford ...
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bioRxiv - Biochemistry 2021Quote: ... and PsnB variants (single concentrations at 0.4-2 μM) were co-incubated at 37°C in a buffer containing ATP (5 mM; Sigma-Aldrich), NADH (400 μM ...
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bioRxiv - Biochemistry 2021Quote: ... cells were starved with 1 ml of -Met medium without ammonium sulfate for 30 min at 30°C in the presence of 100 μg/ml tunicamycin (Sigma) dissolved in DMSO ...
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bioRxiv - Biochemistry 2020Quote: ... Extracted membranes were separated from insoluble debris by centrifugation (130,000 × g for 1 h at 4 °C) and incubated for 2h with 4 mL of Ni2+-NTA beads (Novagen) equilibrated in buffer III (25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... ClpAP degradation reactions with FITC-casein (18 µM) were performed at 30 °C in Buffer HO with 4 mM ATP or ATPγS (Millipore Sigma). Degradation of 5-IAFV13P titinI27-ssrA (5 µM ...
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bioRxiv - Cell Biology 2021Quote: ... Cdc5-3V5 and Rim4-3V5 (control non-kinase tagged protein) were immunoprecipitated at 4°C 2 hours using 20 µL of anti-V5-agarose slurry (Sigma). Purifications were washed 4 times with NP40 buffer.
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bioRxiv - Cell Biology 2021Quote: ... 2×105 cells were seeded in 6-well tissue culture plates and incubated at 37°C for 24 hours before exposure to 4 mM hydroxyurea (HU; Sigma) in regular growth medium for 5 hours ...
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bioRxiv - Microbiology 2020Quote: ... and 2a strains were grown at 37°C in Tryptic Soy Broth (TSB) with shaking or TSB agar plates supplemented with 0.01% Congo Red (Sigma-Aldrich) and Ampicillin (100 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... the same samples were incubated for 2.5 h at 37°C with protein synthesis inhibitor cycloheximide (10 μg/ml) (CHX; Sigma-Aldrich), of which experimental condition was optimized in our previous reports 18,21 ...
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bioRxiv - Plant Biology 2020Quote: ... The precipitations were washed four times with ice-cold immunoprecipitation buffer at 4°C and were analyzed by immunoblot using anti-Myc (Sigma), or anti-GFP (Cell Signaling Technology ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cell pellets were resuspended into BMM-C to an OD600 of approximately 1 and then inoculated into BMM-levan (5 g L-1 levan, Sigma) using an initial OD600 of 0.05 in a 96-well plate (Greiner Bio-One) ...
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bioRxiv - Microbiology 2020Quote: ... digested overnight at 37°C in sodium phosphate buffer supplemented with 100 UI of mutanolysin from Streptomyces globisporus (ATCC 21553, Sigma) and reduced with NaH4B.
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bioRxiv - Microbiology 2020Quote: ... Free dyes and lipids were then purified away from labeled virus particles by ultracentrifugation for one hour at 4 °C at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient ...
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bioRxiv - Evolutionary Biology 2020Quote: ... brucei ALPH1 was expressed as an N-terminal truncation with a C-terminal 6xHis-Tag in RosettaBlue competent bacteria cells (Novagen) as previously described [10].
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bioRxiv - Molecular Biology 2020Quote: ... then diluted to 1×107 cells/ml in liquid YPAD and incubated at 30°C for 2 hours before crosslinking with 1% formaldehyde (Sigma) for 15 minutes followed by quenching with 125 mM glycine for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 30 minutes at 37°C prior to lysis with 2% SDS in PBS containing 5 U/μl benzonase (Sigma). Cell lysates were heated for 10 minutes at 95°C and centrifuged at 21000 x g for 10 minutes at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... mexicana strain M379 (MNYC/BC/62/M379) promastigotes were grown in T-75 flasks at 26 °C in Schneider’s insect medium (Sigma-Aldrich) supplemented with 0.4 g/L NaHCO3 ...
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bioRxiv - Genomics 2021Quote: ... Samples were then centrifugated at 500g for 5 minutes at 4°C and then were resuspended in 1mL Nuclei PURE Storage Buffer (Nuclei PURE storage buffer, Sigma). Sample washing was performed until the supernatant cleared ...
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bioRxiv - Molecular Biology 2021Quote: ... crosslinked to the membrane by chemical treatment at 60°C using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) (Sigma) for 1 h 30 min ...
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bioRxiv - Microbiology 2021Quote: ... The protein interlayer solution in urea was then incubated at 60° C after adding dithiothreitol (Sigma Aldrich, St. Louis, MO) at 5 mM concentration for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... and mutants or recombinants derived from these strains were grown at 37°C in 7H9 medium (Difco) supplemented with 10% albumin-dextrose-catalase (ADC, Difco) and 0.05% Tween-80 (Sigma-Aldrich), or on complete 7H11 solid medium (Difco ...
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CRISPR-Cas9-mediated knockout of CYP79D1 and CYP79D2 in cassava attenuates toxic cyanogen productionbioRxiv - Plant Biology 2021Quote: ... and water purified to a resistivity of 18.2 MΩ·cm (at 25 °C) using a Milli-Q Gradient ultrapure water purification system (Millipore, Billerica, MA) were used to prepare LC mobile phase solvents ...
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bioRxiv - Plant Biology 2021Quote: ... Free hydroxyl FAs were derivatized at 110°C for 30 min in 100 µL of N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA, Sigma) and pyridine (50:50 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Linear pST76-A plasmid was ligated with the PCR fragment using T4 ligase enzyme overnight at 16 °C followed by heat inactivation at 65 °C for 10 minutes and dialysis for 1 h on a 50 nm pore-sized membrane (Millipore). The ligation product was transformed into E ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed with whole-cell extract buffer and the FLAG tagged proteins were immunoprecipitated overnight at 4°C using anti-FLAG-agarose beads (Sigma), as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... All samples were then boiled at 95 °C for 10 mins at a 1:1 (v/v) ratio in 2x Laemmli loading dye (Sigma) before being electrophoresed through a 4-12% NuPAGE Bis-Tris polyacrylamide gel (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... Biofilms were washed twice with 400µl PBS and then incubated in the dark for 45 min at 30°C in 400µl PBS containing 50µg/ml ConA-FITC (Sigma-Aldrich, C7642) and 20µM FUN-1 (Invitrogen Molecular Probes ...
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bioRxiv - Microbiology 2020Quote: ... Amimed). The SVG-A cells (generous gift from C. Hanssen-Rinaldo) were kept in Minimum Essential Medium (M2279, Sigma-Aldrich) including 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... an O/N culture of SS was first incubated at 37 ⁰C in THY with the addition of 100 μM deferoxamine mesylate salt (DIF) [12] (D9533-1G, Sigma) and inoculated the day after in 30 ml aPS containing DIF at the same concentration ...
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bioRxiv - Cancer Biology 2020Quote: ... in 5% BSA-TTBS overnight at 4°C and then HRP-conjugated goat anti-rabbit IgG (Jackson) or goat anti-mouse IgG(Sigma) secondary antibodies for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... on top of 3T3-J2 mouse embryonic fibroblast feeder cells inactivated by mitomycin-C treatment (0.4 μg/ml, Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... antibody-bound fraction was immunoprecipitated for 90 minutes at 4°C using 25 µL slurry salmon sperm protein A agarose beads (Millipore). Unbound proteins were removed by washing the beads 5 times in wash buffer (30 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2020Quote: ... cells were plated onto poly-d-lysine-coated 60 mm cell culture dishes and treated as described above with Ara-C (Sigma) at a final concentration of 5 µM for 48 h ...
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bioRxiv - Cell Biology 2021Quote: 5 mL of yeast cells expressing the indicated constructs (see main text) were grown at 30°C in liquid YNB media (6.8 g/L) (Sigma-Aldrich) supplemented with 5 g/L glucose and 1.92 g/L synthetic drop-out medium without uracil (Sigma-Aldrich ...