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Citations for Millipore Sigma :
3851 - 3900 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... MS-5 cells cultured in αMEM medium at 95 % confluence were incubated with 10 µg/ml mitomycin C (Sigma-Aldrich) for 3 h at 37° ...
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bioRxiv - Developmental Biology 2020Quote: Adult females collected 24-48h after eclosion were fed on yeast at 25°C for two days before dissecting ovaries in Shields and Sang M3 insect medium (Sigma) containing 10% FBS (HyClone ...
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bioRxiv - Molecular Biology 2021Quote: ... CRY1 proteins were purified using Myc resin (EZview™ Red Anti-c-Myc Affinity Gel, SIGMA-ALDRICH Catalog No: E6654) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: ... Setx+/+ and Setx-/- mouse embryonic fibroblasts (MEFs) were cultured at 37°C with 5% CO2 and 5% O2 in DMEM supplemented with 15% FBS (F2442; Sigma) and 1% P/S ...
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bioRxiv - Molecular Biology 2020Quote: ... embryos were cultivated at 28.5 °C for 3 days in fresh standard E3 medium supplemented with 0.05% methylene blue (Sigma Aldrich, MO) and 0.003% phenylthiourea (PTU ...
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bioRxiv - Genetics 2021Quote: ... NMDA receptor subunit 2 C-terminal peptide “SIESDV” was synthesised (Pepceuticals) and fully dissolved in 90% v/v methanol + 1M HEPES pH7 (both Sigma). Dissolved peptide was coupled to Affi-Gel 10 resin (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... the resin was washed 5 times with ice cold DOC buffer and the bound protein eluted by 15 minute 70°C incubation in 5% w/v sodium dodecyl sulphate (SDS, Sigma). The eluted protein was reduced with 10 mM TCEP and alkylated using 20 mM Iodoacetamide ...
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bioRxiv - Plant Biology 2021Quote: ... The solution was left at 4°C on a rotator for 10 min before being filtered twice through Miracloth (Merck Millipore) and spun at 1,000 g for 20 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and then with Laminin from Engelbreth-Holm-Swarm murine sarcome basement membrane (1.2 μg/mL in 1xPBS, 1 h at 37 °C, Sigma-Aldrich). They were passaged every two to three days in an appropriate ratio ...
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bioRxiv - Immunology 2021Quote: ... After digestion for 2 min in a water ice bath the samples were separated by centrifugation at 1000 x g for 30 s at 0°C using a 22 µm filter (Merck, Millipore) and were immediately analyzed by LC-MS ...
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bioRxiv - Molecular Biology 2020Quote: ... 700 ng of the FECH-C minigene (29) was transfected using XtremeGENE HP DNA transfection reagent (Sigma-Aldrich #XTGHP-RO) as per manufacturer’s instructions with a ratio of 2.5 µl transfection reagent per µg plasmid DNA ...
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bioRxiv - Neuroscience 2021Quote: ... were cut using a tissue chopper (McIlwain) and incubated at 35°C with horse serum-containing medium on Millicell culture inserts (CM, Millipore). The medium was replaced every 2-3 days ...
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bioRxiv - Immunology 2020Quote: Primary human NK cells were incubated after enrichment for 16 hours at 37°C in 3 ng/mL IL-15 (Miltenyi) in RPMI media supplemented with 10% Human AB serum (Sigma). Cells were washed with PBS ...
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bioRxiv - Cell Biology 2020Quote: Rat2 fibroblasts and NIH-3T3 mouse fibroblasts were grown at 37°C / 7.5% CO2 in DMEM medium containing 4.5 g/l glucose and 10% FBS (054M3396, Sigma-Aldrich, Germany), 2 mM glutamine ...
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bioRxiv - Physiology 2021Quote: ... was incubated for 2-3 h at 4 °C with 30 µl of FLAG-conjugated beads (anti-DYDDDDDK beads, Sigma) or empty beads (ThermoScientific) ...
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bioRxiv - Neuroscience 2020Quote: ... brains were extracted and swiftly placed in cold (4°C) dissection media consisting of Earle’s Balanced Salt Solution (Sigma-Aldrich) supplemented with D-glucose (6.1 g/L ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then fixed in 0.1M Na+-cacodylate buffer (pH 7.35, prewarmed to 37°C) containing 2% formaldehyde and 0.1% glutaraldehyde (Sigma-Aldrich, Buchs, Switzerland) for 1 h prior light microscopy imaging ...
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bioRxiv - Physiology 2020Quote: ... For insulin clearance during an insulin challenge, mice were given human insulin (1 U/kg, NovoRapid) or human C-peptide (50 µg/kg, Sigma) by intraperitoneal injection and blood samples were collected by tail vein sampling at 0 ...
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bioRxiv - Neuroscience 2021Quote: One series of sections was washed with KPBS to remove the cryoprotectant solution and then incubated with polyclonal rabbit anti-c-Fos (PC-38; Calbiochem-Millipore) at 1:20000 in 0.5% Blocking Reagent (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Synchronized feeding third instar female larvae were sorted and placed in a hole created at the centre of an agar plate (3.3% wt/vol) filled with yeast paste containing 4% (w/v) FD&C Blue dye # 1 (Sigma Aldrich). The larvae were allowed to feed in the dyed-yeast paste for 2 hours ...
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bioRxiv - Immunology 2020Quote: ... 6 days post transfection the cells were spun down (7,000 RPM for 1 hour at 4 °C) and the supernatant was cleared by vacuum filtration (0.45 µm filtration units, Millipore Sigma). Antigen-bearing components were purified using immuno-affinity column with immobilized PGT145 IgG (Sepharose 4B resin ...
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bioRxiv - Neuroscience 2021Quote: ... MhOR5 was eluted by adding 2.5 mM desthiobiotin (DTB) and cleaved overnight at 4°C with HRV 3C Protease (EMD Millipore). Sample was concentrated to ∼5 mg/mL and injected onto a Superose 6 Increase column (GE Healthcare ...
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X-ray mediated scintillation increases synaptic activity via Cerium-doped LSO and Channelrhodopsin-2bioRxiv - Neuroscience 2020Quote: ... Half of the media was removed and replaced after 48h supplemented and with 4μM cytosine 1-β-d-arabinofuranoside (Ara-C; Sigma). Neurons were fed by replacing half the volume of spent media with fresh media without serum or Ara-C every week thereafter.
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bioRxiv - Cancer Biology 2020Quote: ... Photoconverted specimen of each metastatic group was pooled and transferred to a C-column (130-093-237, Miltentyi Biotech) and digested by incubation with 2mg/ml collagenase IV+DNase I (Sigma) in DMEM using the mLIDK program of a gentleMACS (Miltentyi Biotech) ...
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bioRxiv - Immunology 2020Quote: ... Six days post transfection the cells were removed by centrifugation (7000 RPM for 1 hour at 4 °C) and the supernatant was cleared by vacuum filtration (0.45 µm filtration units, Millipore Sigma). BG505 SOSIP-T33_dn2A component was purified from the cleared supernatant using Sepharose 4B resin (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2021Quote: RML12 Aedes albopictus cells with and Wolbachia-free wMel was grown at 24 °C in Schneider’s insect media (Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (Corning) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... free-floating sections were washed with KPBS to remove the cryoprotectant solution and then incubated directly with polyclonal rabbit anti-c-Fos (PC-38; Calbiochem-Millipore) at 1:20000 in 0.5% Blocking Reagent (Roche ...
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bioRxiv - Microbiology 2021Quote: ... up to 5 µg of rCovR was incubated for 60 min at 37°C with 20 mM MgCl2 and 35 mM lithium salt acetyl-phosphate (Sigma), and phosphorylation was confirmed by Western analyses with anti-His tag antibodies after electrophoresis in 12.5 % Phos-Tag SDS polyacrylamide gels (SuperSep Phos-Tag ...
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bioRxiv - Cancer Biology 2021Quote: ... with 1 % tert-butyldimethylchlorosilane were added to 7.5 μL of each derivative and incubated for 60 min at 60 °C (Merck Sigma, 375934)65 ...
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bioRxiv - Neuroscience 2020Quote: N1E-115 mouse neuroblastoma cells were obtained from ATCC and maintained at 37 °C in a humidified atmosphere with 5% CO2 in DMEM (Sigma) supplemented with 10% FBS (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... for 2 hours and stained with primary antibodies overnight at 4°C (guinea pig anti-vGlut2, 1:1000, Millipore AB2251-I; guinea pig anti-vGlut1, 1:1000, Millipore AB5905 ...
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bioRxiv - Neuroscience 2020Quote: ... PCR products were kept at 4°C and then run on a 1.5% agarose gel in TAE (Tris-acetate-EDTA) buffer (Sigma, UK) with the conditional allele showing a larger band (220bp ...
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bioRxiv - Microbiology 2021Quote: ... Colicins B1-341 GFP/mCherry were expressed with a 6xHis tail at the C’ terminus and cloned into the multiple cloning site 2 of a pACYCDuet-1 (Novagen) plasmid where they were expressed under a T7 promotor ...
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bioRxiv - Bioengineering 2020Quote: Porcine carotid arteries were dissected fresh and cryopreserved by freezing at a rate of −1°C per minute in tissue freezing medium (TFM) composed of 0.1M sucrose (Sigma-Aldrich) and 12.75% DMSO (VWR ...
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bioRxiv - Molecular Biology 2022Quote: ... Cross-links were reversed for ChIPs and inputs at 65⁰C overnight with 200 mM NaCl and 2 μl RNase A (Sigma). Samples were then incubated with 20 μg Proteinase K for 1 hr at 45⁰C ...
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bioRxiv - Genetics 2022Quote: ... The slices then incubated with primary antibodies with denoted dilutions overnight at 4°C (anti-Iba1 goat polyclonal, Abcam ab5076, 1:200; anti-HNA mouse monoclonal, Millipore MAB1281 ...
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bioRxiv - Genetics 2022Quote: ... samples were boiled at 70°C for 5 min in 1x NuPAGE LDS sample buffer (Novex NP0007, Thermo-Fischer) with 10% 1M DTT (Sigma) and equal amounts were separated by 4–15% or 8–16% SDS-PAGE (BioRad ...
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bioRxiv - Developmental Biology 2022Quote: ... transgenic and mutant zebrafish embryos were raised at 28°C in Embryo Water (Volvic© water containing 0.28 mg/mL Methylene Blue [M-4159; Sigma] and 0.03 mg/mL 1-phenyl-2-thiourea [P-7629 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged at 15,000 x g for 15 min at 4 °C and the supernatants were separated in SDS–PAGE and transferred onto Immobilon-FL membranes (Millipore). Following blocking with 5% skim milk in PBS containing 0.05% Tween-20 (PBST ...
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bioRxiv - Microbiology 2022Quote: ... NIH clone A2 and the isogenic Δgp63 mutants and their complemented counterparts Δgp63+GP63 (13) were cultured at 26° C in Leishmania medium (M199 [Sigma] supplemented with 10% v/v heat-inactivated fetal bovine serum (FBS) ...
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bioRxiv - Pathology 2022Quote: ... Fibroblast cells and U2OS were cultured at 37°C with 5 % CO2 in growth medium: DMEM medium (Sigma-Aldrich, D6429) supplemented with 10% FBS ...
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bioRxiv - Systems Biology 2022Quote: The cells were fixed with 4% paraformaldehyde at 37 °C for 10 - 15 min and permeabilized using 0.1% Triton-X-100 (Sigma-Aldrich) in PBS for 5 min ...
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bioRxiv - Immunology 2022Quote: ... TZM-bl cells were added to each well and incubated for 48 h at 37°C in presence with 12.5 μg/mL DEAE-Dextran (Sigma-Aldrich). Prior to data acquisition ...
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bioRxiv - Genetics 2022Quote: ... and water purified to a resistivity of 18.2 MΩꞏcm (at 25 °C) using a Milli-Q Gradient ultrapure water purification system (Millipore, Billerica, Massachusetts) were used to prepare LC mobile phase solvents ...
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bioRxiv - Genetics 2022Quote: ... and water purified to a resistivity of 18.2 MΩꞏcm (at 25 °C) using a Milli-Q Gradient ultrapure water purification system (Millipore, Billerica, MA) were used to prepare mobile phase solvents S3 for LC-MS ...
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bioRxiv - Cell Biology 2022Quote: ... Then the cells were fixed with 4% paraformaldehyde at 37 °C for 10 - 15 minutes and permeabilized using 0.1% Triton-X-100 (Sigma-Aldrich) in PBS for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then fixed with 4% paraformaldehyde at 37 °C for 10 - 15 min and permeabilized using 0.1% Triton-X-100 (Sigma-Aldrich) in PBS for 5 min ...
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bioRxiv - Cell Biology 2022Quote: ... The standard labeling conditions involved incubation of cells at 37°C for 120 min with 500 µM biotin (Sigma, B4501), diluted from a 100 mM biotin stock solution made in DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... cells were grown on coverslips and incubated at 37°C for 2 hrs with either actinomycin D (5 μg/ml, Sigma) or DRB (50 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... the cell lysates were centrifuged at 13,000g for 10 min at 4 °C and immunoprecipitated with anti-FLAG M2 affinity gel (Sigma-Aldrich). Immunoprecipitates were resolved by SDS-PAGE ...