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Citations for Millipore Sigma :
3651 - 3700 of 10000+ citations for C C Motif Chemokine Ligand 23 CCL23 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Antibody-bound chromatin was recovered after incubation for ~7 h at 4°C with Magna ChIP Protein A + G Magnetic Beads (16–663, Millipore). Beads were washed twice with Low salt buffer (0.1% SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... and cultured in F-media (62) containing 10 μM Y-27632 Rho-kinase inhibitor (63) in the presence of mitomycin C (M4287; Sigma) treated 3T3 J2 fibroblasts ...
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bioRxiv - Molecular Biology 2021Quote: ... The pellet of fixed cells was resuspended in 1 ml 1x PBS and spread on glass microscopy slides (previously incubated at 180°C for 2h for RNAse removal) within circles of hydrophobic barriers (PAP pen, Sigma). The cells were allowed to settle at room temperature for 20 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then incubated overnight in EtOH 100% at 4°C prior to clearing with ethyl cinnamate (Sigma, 112372-100G) at RT for at least 2 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were then washed in PBS-T and blocked overnight at 4 °C in PBS-T with 1 % bovine serum albumin (BSA, Sigma) and 5 % donkey serum (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... encoding PPK with a C-terminal GAAEPEA peptide tag for affinity purification (105) between the NdeI and HindIII sites of plasmid pET-21b(+)(Novagen), was synthesized by GenScript.
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bioRxiv - Developmental Biology 2020Quote: ... transgenic and mutant zebrafish embryos were raised at 28°C in Embryo Water (Volvic© water containing 0.28 mg/mL Methylene Blue [M-4159; Sigma] and 0.03 mg/mL 1- phenyl-2-thiourea [P-7629 ...
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bioRxiv - Developmental Biology 2020Quote: ... Ovary of both medaka and trout were fixed overnight at 4°C by 4% paraformaldehyde (PFA) in 0,01 M phosphate buffer saline pH 7.4 (PBS, Sigma-Aldrich P4417). Ovaries were washed in PBS and conserved in PBS + 0.5% (w/v ...
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bioRxiv - Developmental Biology 2021Quote: ... The embryos were then washed through pre-warmed (37°C) Hank’s balanced salt solution (HBSS, Sigma-Aldrich; Cat. No. H9269) and lysed by moving to a 1.5ml centrifuge tube containing about 15µl of SDT-lysis buffer (4% (w/v ...
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bioRxiv - Immunology 2022Quote: ... 1.000 to 50.000 cells were allowed to adhere for 1 hour at 37°C to coverslips coated with 5 μg/cm2 poly-D-Lysine (Sigma). Cells were then washed in PBS 1x ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were allowed to grow for 96 h in drug containing medium (methyl methanesulfonate, Acros Organics 156890250, mitomycin C, Sigma M4287 ...
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bioRxiv - Cell Biology 2022Quote: ... at 37°C for 15 min and then washed with 3x times in fresh DMEM followed by incubation with Hoechst 33342 (Sigma) for 5 min and further analyzed by confocal imaging ...
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bioRxiv - Neuroscience 2022Quote: ... Virgin females and males used in optogenetic assays were raised in darkness at 25°C on food containing 0.2 mM all-trans- Retinal (Sigma-Aldrich) before and after eclosion ...
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bioRxiv - Plant Biology 2022Quote: ... The sample was then centrifuged twice at 13000 g for 10 min at 4°C and the supermnatent was passed through Millex® GV 0.22 μm PVDF membrane filter (Merck Millipore). Next ...
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bioRxiv - Plant Biology 2022Quote: ... We used an Ascentis Express C-18 Column (3 cm × 2.1 mm, 2.7 µm) (Sigma-Aldrich, St. Louis, MO, USA) connected to an API 3200 LC-MS/MS (Sciex ...
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bioRxiv - Microbiology 2022Quote: ... pneumoniae was grown at 37°C on LB agar plates supplemented with 3 μg/mL potassium tellurite (Sigma-Aldrich, USA).
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bioRxiv - Microbiology 2022Quote: ... lentiviruses were thawed at 37°C and added to target cells in complete media containing 5 µg/mL polybrene (Sigma) for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... DLD-1-WT mCherry-Sec61β and DLD-1-C-H3 mCherry-Sec61β stable cell lines were generated by GeneJuice (Merck Millipore) transfection of mCherry-Sec61β into the respective parental lines ...
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bioRxiv - Microbiology 2022Quote: ... lentiviruses were thawed at 37°C and added to target cells in complete media containing 5 µg/mL polybrene (Sigma). After 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... DRGs were centrifuged for 4 min at 500 xg at 4°C and incubated in 1 ml dissociation buffer (2.5mg/ml collagenase, Sigma Aldrich, C9722 ...
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bioRxiv - Neuroscience 2020Quote: ... the 1st instar larva expressing myr-GCaMP5a in astrocytes (25°C, 24-32 hours after egg laying) was sandwiched in 30μl halocarbon oil 27 (Cat# H8773, Sigma-Aldrich) between a slide and a 22 × 22mm coverslip (Cat# 1404-15 ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated at 4 °C overnight in the same solution with the primary rabbit anti-OMP (olfactory marker protein; 1:500, O7889 from Sigma). Immunofluorescence was achieved by reaction with the secondary antibody donkey anti-rabbit-488 (A21206 from Molecular Probes ...
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bioRxiv - Neuroscience 2021Quote: ... maintained for at least 1h at 4°C and then transferred onto semiporous membranes inserts (47 mm diameter, 0.45 µm pore size; Millipore FHLP04700) which were placed in six well tissue culture plates containing 1.1 ml medium per well ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared and then immediately enzymatically treated at 34°C with an initial incubation in a papain (6 mg/mL, Sigma) and dithioerythritol (10 mg/mL ...
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bioRxiv - Neuroscience 2021Quote: Rat recombinant RIIβ was derived as a C-terminal 6XHis fusion by Ni-NTA affinity purification using standard pET vector methods (Novagen). RIIβ was phosphorylated in vitro by Cdk5 or PKA using standard conditions (Bibb et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were reduced in 50 mM ammonium bicarbonate with 10 mM DTT for 30 min at 37°C and alkylated in 55 mM iodoacetamide (Sigma) for 20 min at ambient temperature in the dark ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1 M NaHCO3) and cross-linking was reversed overnight at 65°C with 200 mM NaCl and 2 μL RNase A (Sigma). A matched input sample (1/10 of original ChIP reaction ...
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bioRxiv - Molecular Biology 2020Quote: ... The transformation reaction was incubated at 30°C for 10 minutes before adding 500μL Polyethylene Glycol (PEG, Sigma P4338-1KG) and another incubation at 30°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The EDL muscle was next incubated for 105 minutes at 37 °C in 0.2% collagenase (Sigma Aldrich, Zwijndrecht, the Netherlands) in DMEM medium (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... Samples were heated for 5 min at 95°C prior to loading on 12% polyacrylamide gels followed by transfer onto NC membrane (Millipore). 3% BSA in phosphate buffer saline (PBS ...
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bioRxiv - Genomics 2020Quote: ... Undifferentiated SH-SY5Y cells were cultured in growth media under standard conditions (5% CO2, 37°C) with DMEM:F12 (Sigma D6421), supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... the amount needed for a single day was dissolved in 95% ethoanol with heating to 37°C and then diluted in corn oil (Sigma) such that 100μL had 5mg.
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bioRxiv - Molecular Biology 2021Quote: ... ChIP DNA was eluted in ChIP elution buffer (1% SDS, 100mM NaHCO3) and reversed cross-linked overnight at 65°C with 200mM NaCl and RNase A (Sigma). The reverse cross-linked samples were treated with 20 μg/ml Proteinase K and purified using ChIP DNA Clean and Concentrator kit (Zymo research) ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were eluted in Laemmli buffer by boiling at 95°C for 5min for Western blot or with 3xFlag peptide (150 µg/ml, Sigma) for 1 hr at 4°C for HAT assay ...
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bioRxiv - Molecular Biology 2019Quote: Embryos were incubated in 1:5000 acridine orange solution for 1 hr at 28.5°C (stock: 6 mg/ml, Sigma-Aldrich) followed by 2X washes in fish system water ...
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bioRxiv - Molecular Biology 2019Quote: ... for 2 hours at 4°(C) Immunocomplexes were washed 5× with S300 buffer and eluted by competition with 1x Flag peptide (500 ng/ul; SIGMA) 2 times for 30 min at 16 °C and then resuspended in Laemmli sample buffer ...
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bioRxiv - Genomics 2019Quote: ... 1mM EDTA) and reverse crosslinked by incubation for overnight at 65°C and subsequent incubation with 5 µl RNAse (Sigma) for 1h at 37°C and Proteinase K Solution (20 mg/mL ...
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bioRxiv - Developmental Biology 2019Quote: ... All animals were incubated at 28.5 °C for 5 h before treatment with 1-phenyl-2-thiourea (PTU) (Sigma Aldrich) to prevent pigment formation ...
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bioRxiv - Biochemistry 2020Quote: ... DI water (18.2 MΩ•cm at 25°C) was provided in-house by a Synergy Ultrapure Water System (EMD Millipore).
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bioRxiv - Molecular Biology 2019Quote: ... 0.1 M NaHCO3) and cross-links were reversed overnight at 65°C with 200 mM NaCl and 2 μl RNase A (Sigma). A matched input sample (10% of original ChIP reaction ...
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bioRxiv - Genomics 2019Quote: ... The reaction was carried out at 60°C for 2 hours in a custom labeling buffer with a final concentration of 50 mM K-acetate (Sigma), 10 mM Mg-Acetate (Sigma) ...
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bioRxiv - Microbiology 2019Quote: Strains were grown overnight on TSA plates and used for inoculating TSB and allowed to grow to OD600 = 0.05 when Mitomycin C (MMC from 5treptomyces caespitosus, Sigma-Aldrich) was added ...
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bioRxiv - Genetics 2019Quote: ... USA) for 30 minutes at 37°C to remove any residual DNA and dissolved in DEPC (Diethyl pyrocarbonate, Sigma, USA) treated water ...
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bioRxiv - Microbiology 2019Quote: ... except both types of biofilms were incubated for 24 hours in 37°C and inoculated 1:100 medium was supplemented with 50µg/ml hyaluronidase (cat#H3506, Sigma-Aldrich). Following incubation ...
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bioRxiv - Microbiology 2019Quote: ... mexicana strain M379 (MNYC/BC/62/M379) were grown in T-25 flasks at 26 °C in Schneider’s Insect Medium (Sigma-Aldrich) supplemented with 0.4g/L NaHCO3 ...
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bioRxiv - Cell Biology 2019Quote: ... Flag-KIF4A complexes were isolated by 2h incubation at 4°C with 15µl anti-Flag M2-agarose beads (Sigma-Aldrich). Beads were washed twice with 1ml lysis buffer and twice with 1ml wash buffer (20mM Tris-HCl pH7.4 ...
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bioRxiv - Microbiology 2019Quote: ... of Saksenaea dorisii strain BiMM-F232 (collected from CZA grown at 30°C for 14 days into physiological solution) was evenly distributed on RPMI-1640 (Sigma) medium plates ...
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bioRxiv - Systems Biology 2020Quote: ... using tube holders pre-cooled at -20 °C and adding the same volume of acid-washed glass beads (Sigma-Aldrich), for 5 min at 30 Hz ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were hydrated with H2O for 3 min at 37°C followed by treatment of the tissue with 0.5 mg/ml of Pepsin (Sigma-Aldrich) for 15 min at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells that had invaded the underside of the inserts were fixed with 100% methanol for 10 minutes at −20°C and stained with 0.5% crystal violet dye (EMD Millipore) for 10 min at room temperature ...