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Citations for Millipore Sigma :
2751 - 2800 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Astroglia proliferate upon biogenesis of tunneling nanotubes and clearance of α-synuclein toxicitiesbioRxiv - Cell Biology 2023Quote: ... was prepared with unlabelled α-SYN wild-type and labelled α-SYN-141C by incubating with 0.65% 4-Hydroxynonenal (10mg/ml stock) (Sigma-Aldrich #393204-1MG) at 37°C for 7 days with moderate shaking (Sackmann ...
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bioRxiv - Neuroscience 2022Quote: ... endothelial cells were plated onto the top of 0.4 μm Transwell inserts (Becton Dickinson, New Jersey, USA) pre-coated with 10 μg/cm2collagen type IV (Sigma-Aldrich, St. Louis, MO, USA) and 5 μg/cm2fibronectin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: Cells treated with or without nicotine were rinsed with PBS then seeded in low-gelling temperature Agarose Type VII (Sigma Aldrich, St. Louis, MO). The cells were seeded in triplicate in 6-well plates (5,000 cells/well ...
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bioRxiv - Neuroscience 2024Quote: As previously described 51 type 1 diabetes was induced by two low dose injections of streptozotocin (i.p., STZ; Sigma# S0130; 75mg/kg per injection) dissolved in 50mM (pH 4.5 ...
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bioRxiv - Cell Biology 2023Quote: ... 4g of tissue/10ml digest solution [phenol-free DMEM [high glucose + 25mM HEPES] (10ml total per C-Tube) with 1mg/ml Type 1 Collagenase (Sigma Aldrich; catalog no. SCR103), 1U/ml Dispase (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... six-to eight-week-old ΔSPC and wild type FVB/N mice were intraperitoneally (i.p.) injected with urethane (1 mg/g body weight, Sigma-Aldrich, St. Louis, MO, USA) once a week for six consecutive weeks [6] ...
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bioRxiv - Biophysics 2024Quote: ... cells were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA) and maintained in Dulbecco’s Modified Eagle Medium (DMEM) (D5796, Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: All plates required for cell culture were coated with 100µg/mL with collagen rat tail type I (20nM acetic acid/distilled water) overnight before seeded with cells (Sigma-Aldrich; UK, ThermoFisher Scientific; USA). Spinal cords were collected from postnatal 7–14 day old mice and placed in Hanks balance buffer solution (Gibco ...
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bioRxiv - Biochemistry 2023Quote: McArdle RH7777 rat hepatocytes (McA cells) stably expressing human apoB100[31] were grown in culture plates coated with type I collagen from calf skin (Sigma Aldrich, St. Louis, MO) as previously described[32] and cultured in growth media composed of Dulbecco’s modified – Eagle’s medium (DMEM ...
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bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
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bioRxiv - Neuroscience 2022Quote: ... Muscle tissues were minced into small pieces with scissors and added to a 50mL conical tube with 1mL of enzyme mix (500 U/mL type II collagenase [MP biomed], 1.5U/mL Collagenase D [Sigma] 2.5U/mL Dispase II [Sigma], 2.5mM CaCl2, PBS up to 1mL). The mixture was shook at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: DNA encoding wild-type and the mutant version of PLIN3 were cloned into BamHI and NdeI restriction sites of pET16b vector (Novagen, Merck, Kenilworth, NJ, USA) using Gibson Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: C57BL/6J wild type and LDLR KO (B6.129S7-Ldlrtm1her/J) mice were intraperitoneally injected by 3% brewer thioglycollate medium (Millipore-Sigma, St. Louis, MO, USA). Mouse peritoneal cavity elicited macrophages (MØ ...
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bioRxiv - Biophysics 2023Quote: ... and human embryonic kidney 293 (HEK293) cells were obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA) and maintained in DMEM (D5796, Sigma-Aldrich, St. Louis, MO), which was supplemented with 10% FBS (12676029 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... an isolated infrarenal aortic segment of C57BL/6J mice was perfused with sterile isotonic saline containing type I PPE (2-3 U/ml, E1250, Sigma-Aldrich, Burlington, MA, USA) for 5 min under pressure (120 mmHg ...
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bioRxiv - Neuroscience 2024Quote: ... Pellets were re-suspended and incubated with either a monoclonal primary antibody against collagen type VI (Merck Millipore, Burlington, MA, US, MAB1944, 1:250) or with a monoclonal primary antibody against fibroblast surface protein (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mL/well cell-virus suspension was seeded into 50 µg/mL rat tail collagen type I (Sigma Aldrich, St. Louise, MO USA) coated 6-well plates ...
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bioRxiv - Biochemistry 2024Quote: ... the isolated membranes were solubilized as mentioned before (Winklemann et al., 2020) with addition of brain extract from bovine brain type VII (Sigma-Aldrich, cat. nr. B3635) to a total concentration of 0.003mg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... R8781-200UL), type I collagenase (Cat. No. C0130), and Fetal bovine serum (FBS, Cat. No. F8317) were purchased from Sigma-Aldrich (St. Louis, MO). TGFβ1 recombinant protein (Cat ...
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bioRxiv - Cell Biology 2023Quote: ... anti-rabbit anti-chicken and anti-sheep were purchased from Sigma.
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bioRxiv - Plant Biology 2020Quote: ... 1 ml of transfected protoplasts were incubated for 12 h for protein expression in the presence of 1 μM DSF (Sigma, USA), and were treated with or without 1 μM flg22 (MoonBiochem ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were separated by SDS-PAGE on a 4-12% Bis Tris gel (NuPage, NP0335BOX) and transferred onto a polyvinylidene fluoride membrane (Millipore, IPVH00010). Membranes were blocked with 5% bovine serum albumin (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... ARPE-19 cells were seeded at semi-confluence in 12-wells plates and next day medium was replaced for growth medium containing 2mM thymidine (Sigma-Aldrich). After 24 hrs ...
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bioRxiv - Cell Biology 2020Quote: Macrophages were differentiated from THP1 cells using serum-free DMEM supplemented with 10 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich). After 48h ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were boiled and loaded on a 12% SDS-polyacrylamide gel and blotted onto a PVDF membrane (Immobilon®-P transfer membrane, Millipore). The blots were blocked for 1 h in PBS containing 0.05% Tween-20 and 3% BSA at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Samples were heated to 50°C for 15 min before being loaded on 12% TruPAGE Tris-Glycine Precast gels (Sigma Aldrich). Each assay was triplicated ...
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bioRxiv - Cell Biology 2021Quote: ... Ultrathin sections were stained with 4% uranyl acetate for 12 min in dark at room temperature and citrate mixture (SIGMA-ALDRICH) for 2 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... total RNA was isolated from iPSC cultures driven towards glutamatergic deep cortical neurons or GABAergic neurons at time points along the differentiation protocol spectrum (Days 0, 12, 35, 60, and 35+14; Trizol, Sigma-Aldrich). Total RNA was reverse transcribed and used for quantitative RT-PCR with primers specific to Sox2 ...
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bioRxiv - Neuroscience 2021Quote: ... 2D viability control cultures were seeded in 12-well cell culture plates on wells coated with poly-D-lysine (Sigma-Aldrich) at a density of 288,000 cells/well.
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bioRxiv - Neuroscience 2021Quote: ... GCaMP6f-expressing HEK cells were seeded on 12-well cell culture plates with 18mm glass coverslips coated with PDL (10μg/μl; Sigma-Aldrich P6407) for 1-2h ...
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bioRxiv - Molecular Biology 2020Quote: One third of samples from the IPMS experiment was loaded to a 4-12% SDS PAGE and the silver staining was carried out with ProteoSilver™ Plus Silver Stain Kit (Sigma). Each visible band was cut for trypsin digestion and MS identification ...
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bioRxiv - Immunology 2022Quote: ... cells were seeded at a density of 1 × 106 cells per well in 12-well plates and stimulated with LPS (100 ng/mL, M1-like - Sigma-Aldrich), IL4 (10 ng/mL ...
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bioRxiv - Bioengineering 2020Quote: ... was obtained with institutional ethical approval (HIREB No. 12-085-T) and fixed in a 4% glutaraldehyde (Sigma Aldrich, Missouri, US) solution in a 0.1 M cacodylate buffer for 7 days ...
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bioRxiv - Neuroscience 2021Quote: Astrocytes were pretreated for 2 h with 5% charcoal-stripped FBS DMEM/F-12 medium and incubated with DMSO vehicle or in the presence 1 nM 17-estradiol (Sigma-Aldrich) in 5% charcoal-stripped FBS DMEM/F-12 medium for 1 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: For methimazole treated samples, Adult C57BL/6J mice (6-12 weeks old, JAX stock No. 000664) were given intraperitoneal injections with Methimazole (Sigma M8506) at 50 μg/g body weight and sacrificed at 24 ...
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bioRxiv - Biochemistry 2020Quote: ... Reaction mixture consisted of freshly thawed 12 µM purified bovine brain tubulin in microtubule growth buffer (BRB80, 1 mM GTP (Millipore Sigma), 5 mM DTT (Millipore Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... samples were resolved under denaturing and reducing conditions using 4-12% Bis-Tris gels (NuPAGE®) and transferred to a PDVF membrane (Immobilon-P, Millipore). Membranes were blocked with 5% nonfat dried milk ...
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bioRxiv - Neuroscience 2020Quote: Stereotaxic cannulation surgeries were performed in animals anesthetized with a ketamine/xylazine combination (80/12 mg/kg, i.p.; Western Medical Supply/Sigma, Arcadia, CA). Bilateral cannulation of the ACC was performed as previously described (Johansen et al ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were plated at a density of 2 x 105 cells on 12 mm cover glass coated with PLL and were maintained in DMEM/F12 (Sigma-Aldrich) containing 100 μM putrescine ...
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bioRxiv - Neuroscience 2020Quote: ... transfected cells were detached by phosphate-buffered saline (PBS) containing 5 mM EDTA and seeded on 12-mm coverslip coated with poly-L-lysine (PLL; Sigma-Aldrich) at 2 x 104 cells/well ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein samples were resolved by electrophoresis on 12% SDS–polyacrylamide gels and electrophoretically transferred to polyvinylidene difluoride (PVDF) membranes (Millipore Corporation) for 20 min at 25 V with the Trans-Blot Turbo (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2021Quote: ... Coring 6.5 mm Transwell with 8.0 um pore polycarbonate membrane insert in 12 well plates were used (Sigma Aldrich, cat# CLS3422). The insert membrane was treated for optimal cell attachment ...
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bioRxiv - Cancer Biology 2020Quote: ... H3255 and H3255-TM were maintained in ACL4 medium formulated in DMEM:F-12 including: Bovine Serum Albumin 0.5% (w/v) (Sigma cat no. A8022), apo-Transferrin (human ...
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bioRxiv - Bioengineering 2020Quote: ... bone-marrow derived macrophages were seeded at 100,000 cells per 3.5 cm2 in a tissue-treated 6 well plate and incubated in 500 uL DMEM/F-12 media containing 10 ng/mL lipopolysaccharide (LPS) (Cat. No. L4391; Sigma Aldrich) to skew the cells towards a pro-inflammatory M1 macrophage phenotype ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was done in ATP hydrolysis buffer (1 mM DTT, 4 mM MgCl2, 1 mM PEP, 12 µL LDH/PK enzyme mix (Sigma Aldrich), 50 mM HEPES (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... Twenty micrograms of each sample was run on NuPAGE 4–12% Bis-Tris and proteins were electroblotted onto PVDF membranes (Millipore, ISEQ00010), which were further processed as described in the immunoblotting section.
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bioRxiv - Cell Biology 2021Quote: ... 1 µg of RNA was reverse transcribed using Oligo (dT) 12-18mer primers and M-MLV Reverse Transcriptase (Sigma-Aldrich #M1302) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM DTT using Slide-A-Lyzer (Pierce Inc.) and concentrated to 10-12 mg/ml using Centricon 50 (Millipore Inc.). A typical yield was ~1 mg protein/liter culture ...
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bioRxiv - Microbiology 2020Quote: ... cells were plated on 24-well plates at 3×105 cells/well with 0.1 µg/mL 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich #P1585) to promote differentiation ...
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bioRxiv - Neuroscience 2021Quote: ... 40μg of total proteins from each sample were separated by 8∼12% SDS-PAGE and transferred onto PVDF membranes (Millipore, USA), the membranes were blocked in 5% BSA ...