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Citations for Millipore Sigma :
2651 - 2700 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Lower layers of S1 were dissected out and immediately homogenised in ice-cold lysis buffer (50 mM Tris-HCl pH 7.5, 100 mM KCl, 12 mM MgCl2, 1 mg/ml Heparin (Sigma-Aldrich), cOmplete EDTA-free protease inhibitors (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... samples were resolved under denaturing and reducing conditions using 4%–12% Bis-Tris gels (NuPAGE) and transferred to a PVDF membrane (Immobilon-P, Millipore). Membranes were blocked with 5% nonfat dried milk ...
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bioRxiv - Immunology 2023Quote: ... isolated colon LPLs from individual mice were transferred to 48- well plates and restimulated with conditioned RPMI media containing 500ng/ml Ionomycin (Calibiochem) and 50ng/ml Phorbol 12-myristate 13-acetate (Sigma) for 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... U2OS cells were transfected 12–24 h after seeding into eight-well Imaging Chamber CG (Zell-Kontakt) with XtremeGENE HP (Sigma) according to the manufacturer’s instructions and incubated for 48 h prior to imaging.
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was mixed with loading dye and separated on SDS -12% polyacrylamide gel and transferred onto PVDF Membrane (Millipore) before incubation with rabbit anti-OPTN (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... THP-1 monocytes were seeded (20,000 cells/well) in medium containing 25 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) for 48 h and afterwards rested for 24 h ...
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bioRxiv - Neuroscience 2024Quote: Synchronized adult worms were exposed to Phorbol 12-myristate 13-acetate (PMA, 0.05 mg/ml, Catalog No. P8139, Sigma-Aldrich) or an ethanol solvent as a control ...
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bioRxiv - Neuroscience 2024Quote: ... The brains were post-fixed in the same solution for 12 h at 4°C and then cryoprotected for at least 24 h in 30% sucrose (Sigma) in 1× PBS at 4°C ...
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bioRxiv - Biochemistry 2024Quote: 10 µM His6-HT2 reacted with 12 µM HyT36 or in presence of 12 µM HyT36(-Cl) or DMSO was incubated with 0.1 µM trypsin (Sigma-Aldrich) for 15 ...
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bioRxiv - Developmental Biology 2024Quote: ESCs were seeded onto fibronectin-coated 12-well plates (16.7μg/ml fibronectin [Millipore; FC010] at 37°C for 1 hour) at a density of 1×105 cells/well ...
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bioRxiv - Cell Biology 2024Quote: ... 25-30µg protein was loaded on resolving 12% SDS-PAGE and later transferred to a methanol-activated PVDF membrane (Millipore-# IPVH00010) by using a semi-dry Trans blot apparatus (Bio-Rad) ...
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bioRxiv - Immunology 2023Quote: ... Intracellular staining was performed after stimulation of single-cell suspensions with Phorbol 12-myristate 13-acetate (PMA at 50 ng mL−1, Sigma), ionomycin (0.5 μg mL−1 ...
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bioRxiv - Immunology 2024Quote: CNS and splenic cell suspensions were stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich, #P8139), 1 µM Ionomycin (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Glycoaldehyde phosphate barium salt was synthesized in an aqueous 150 mL reaction by partially dissolving 12 mmol (2.33 g) glycerol phosphate magnesium salt hydrate (Sigma #17766) and 11 mmol sodium meta-periodate (2.35 g) ...
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bioRxiv - Cancer Biology 2024Quote: ... TRACER scaffolds were manufactured by infiltrating cellulose paper strips (0.5 x 12 cm, Miniminit Products) with poly(methyl methacrylate) (PMMA, 120 kDa) dissolved in acetone (Sigma-Aldrich) at 0.175 g/mL with an Allevi Beta 1 3D printer ...
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bioRxiv - Bioengineering 2024Quote: ... THP-1 were seeded into a 6 well plate at 2 x 105 cells/well and treated with 20 ng/mL phorbol-12-myristate 13-acetate (PMA, Sigma) in RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: Approximately 100 µg protein of each sample was separated by 12% SDS-PAGE and transferred to an Immobilon-PVDF membrane (Merck Millipore). The blot was blocked with 5% non-fat milk in 1× TBST (20 mM Tris base ...
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bioRxiv - Microbiology 2023Quote: THP-1 cells were seeded at 5 ×105/mL in 24 well plate and differentiated to macrophage-like cells by treatment with 200 nM Phorbol 12-myristate 13-acetate (PMA; Sigma) for 18 hrs ...
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bioRxiv - Molecular Biology 2023Quote: ... Fbp1F/F and Fbp1ΔHep mice that were HFD fed for 12 wk or NCD-8 wo mice were fasted for 12-14 h and then given 1 g/kg glucose or sodium pyruvate (2 g/kg, Sigma) by i.p ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were resolved under denaturing and reducing conditions using 4 to 12% bis-tris gels (NuPAGE) and transferred to a polyvinylidene fluoride membrane (Immobilon-P, Millipore). Membranes were blocked with 5% nonfat dried milk and incubated with CAMKIIα (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... and immediately homogenised in ice-cold homogenisation buffer (50 mM Tris-HCl pH 7.5, 100 mM KCl, 12 mM MgCl2, 1 mg/ml Heparin [Sigma-Aldrich] ...
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bioRxiv - Neuroscience 2023Quote: ... 70,000 neurons were plated onto 12 mm glass coverslips coated with 10 μg/mL poly-D-lysine (PDL, Sigma P6407) and 2 μg/mL laminin ...
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bioRxiv - Immunology 2023Quote: ... Interphase cells were collected and incubated at 37°C in 5% CO2 for 6 hours with 20ng/ml Phorbol 12-Myristate 13-Acetate (PMA, Sigma), 1µg/ml Ionomycon (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... in 300μl of Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12, GibcoTM) + 5 % goat bovine serum (GBS, Sigma Aldrich) + 1% penicillin-streptomycin (10 000 U/ml ...
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bioRxiv - Microbiology 2023Quote: ... followed by an introduction of 0.12 mL nitrate stock solution (40 g N L-1) which contained ∼1% 15N-labelled NO3- (98 atom % 15N, Sigma). The butane culture was flushed with argon gas (99.99% ...
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bioRxiv - Immunology 2023Quote: ... total tissue homogenate/digest was directly re-stimulated with 50 ng/ml phorbol-13-myristate-12-acetate (PMA) (Sigma-Aldrich) and 0.5 μg/ml ionomycin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... in antibiotic-free media at 2 M cells per well of a 12-well plate that had been coated with 0.1% gelatin (Sigma, G2500) for 15 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... THP-1 cells were stimulated with either 100ng/mL (for M1 macrophages) or 50ng/mL (for M2 macrophages) of phorbol 12-myristate 13-acetate (PMA, Sigma) for 48h ...
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bioRxiv - Cancer Biology 2023Quote: ... containing a 50% mix of L-WRN fibroblast cell line conditioned media (CM) and primary culture medium (Advanced DMEM/F-12; Sigma) both supplemented with 20% fetal bovine serum (Atlanta Biologicals) ...
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bioRxiv - Molecular Biology 2023Quote: ... the titrations of total protein were separated on 12% SDS-PAGE gel followed by blotting to Immobilon PVDF membrane (Millipore). Membranes were dried and total protein was stained with Sypro Ruby (Thermo-Fisher ...
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bioRxiv - Microbiology 2023Quote: ... The LPS extracts were loaded into a NuPAGE 4-12% Bis-Tris Gel (NP0335BOX) along with commercially available LPS (Sigma) derived from E ...
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bioRxiv - Plant Biology 2023Quote: ... Fractionated proteins were separated on NuPAGE 4-12% Bis-Tris mini gels (Life Technology) and transferred onto Immobilon-P membranes (Millipore) for immunoblotting analysis ...
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bioRxiv - Cell Biology 2023Quote: ... Agonist or vehicle was added to cells grown in 12-well plates coated with 0.001% (w/v) poly-L-lysine (Millipore Sigma) and incubated at 37 °C and 5% CO2 as indicated in figure legends ...
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bioRxiv - Microbiology 2023Quote: ... Samples were separated by SDS-PAGE on a 12% (vol/vol) acrylamide gel and transferred to polyvinylidene difluoride (PVDF) Immobilon-P membranes (Millipore). Primary antibodies specific for human IL-18 (MLB International PM014) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured with lentiviral solution for 8-12 h in the presence of 8 μg/mL Polybrene (Sigma- Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... Cat# CRL-2266) were grown on culture dishes in DMEM/F-12 with 10% fetal bovine serum (Sigma, Cat# F4135) at 37 °C in humidified air with 5% CO2 ...
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bioRxiv - Genomics 2022Quote: ... cell culture media was changed to hepato-medium (DMEM:F-12, supplemented with 10% FBS, 1 μg/ml insulin (Sigma-Aldrich), 100 nM dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: Samples were loaded on a NuPAGE 4-12% Bis-Tris protein gel and transferred onto an Immobilion PVDF membrane (Merk-Millipore) for 1 h at 35 V in a wet transfer apparatus buffered with 12.5 mM Trizma-Base (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates containing 50 µg protein were separated by electrophoresis on 8–12% polyacrylamide gels and the separated proteins were then electrotransferred to 0.45 μm PVDF membranes (#IPVH00010, Millipore, USA) at 200 mA for 2 h ...
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bioRxiv - Microbiology 2023Quote: MDCK cells (2 x 105 cells) were cultured on polycarbonate Millicell culture plate inserts (12- mm diameter, 3-µm pore size; Merck Millipore) at 37°C under 5% CO2 atmosphere.
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bioRxiv - Neuroscience 2023Quote: ... 70,000 neurons were plated onto 12 mm glass coverslips coated with 10 μg/ml poly-D-lysine (PDL, Sigma P6407) and 2 μg/ml laminin and incubated at 37°C in 10% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... with a molecular weight cut-off (MWCO) 12–14 kDa and concentrated to 5 mg/ml using spin-filters (Centriprep, Millipore) with a MWCO of 10 kD ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were plated onto Agilent Seahorse XF96 cell culture plates or 12-well cell culture dishes coated with 0.1% gelatin (Sigma #G1393) in DMEM/F12 medium (Gibco #11330057 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were fixed for 12 min at room temperature (RT) and quenched by replacing the fixation solution with freshly made 0.2% NaBH4 (Sigma Aldrich) in PBS for 3 min ...
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bioRxiv - Bioengineering 2023Quote: ... 50 µl of 12 M HCl was added to the dried pellet followed by 56 µl of 10 M sodium hydroxide (Sigma), 100 µl of 4 M ammonium acetate (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... TIGIT-/- and TIGIT-/-ICOSL-/- NOD mice were incubated 4 hours at 37°C with Phorbol 12-myristate 13-acetate (PMA 10 ng/ml; Sigma), ionomycin (500 ng/ml ...
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bioRxiv - Molecular Biology 2023Quote: Glycerolipid content of male and female whole bodies and female ovaries and fat bodies was measured 12 days after dsGFP and dsXPO1 microinjection using the colorimetric Serum Triglyceride Determination kit (Sigma, Triglyceride Reagent T2449 ...
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bioRxiv - Immunology 2023Quote: ... Lipopolysaccharides from Escherichia coli 055: B5 (L2880) and Phorbol 12-myristate 13-acetate (PMA) (P1585) were obtained from Sigma-Aldrich. Yoda1 (5586 ...
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bioRxiv - Immunology 2023Quote: ... of Escherichia coli O111:B4 or O127:B8, and Phorbol 12-myristate 13-acetate (PMA, CAS: 16561-29-8) were obtained from Sigma. Adenosine 5’-triphosphate disodium salt (ATP ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were run on a 12% Tris-acetate SDS-polyacrylamide gel and transferred to a nitrocellulose membrane (Sigma−Aldrich). Membranes were blocked in TBS containing 0.1% Tween-20% and 5% nonfat dry milk for 2 h at room temperature ...