Labshake search
Citations for Millipore Sigma :
2851 - 2900 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Dissociated cells from one embryonic heart were plated on one glass coverslips (diameter 12 mm) pre-coated with bovine fibronectin (10 μg/ml in PBS; Sigma-Aldrich) in 50 μl DMEM/10% FCS/penicillin/streptomycin at 37 °C in a humidified atmosphere of 95% air and 5% CO2 ...
-
bioRxiv - Immunology 2022Quote: ... T cells were stimulated with 50 ng/ml phorbol 12-myristate 13-acetate (PMA) and 500 ng/ml ionomycin (Sigma-Aldrich) in the presence of GolgiStop (BD ...
-
bioRxiv - Immunology 2022Quote: ... THP-1 monocytes are differentiated into macrophages by 36 h incubation with 20 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139). The murine macrophage Raw 274.7 cells (ATCC ...
-
bioRxiv - Genetics 2022Quote: Female XEN cell line was grown on 0.2% gelatin-coated flasks following the Rossant lab XEN stem cell protocol (https://lab.research.sickkids.ca/rossant/wp-content/uploads/sites/12/2015/08/XEN-Stem-Cell-protocol1.pdf) in serum-containing XEN medium (RPMI 1640 (Sigma, M3817)) ...
-
bioRxiv - Genetics 2022Quote: ... 30 adult worms were dropped in 10 μl S-basal in a well of a Corning Costar® 12-well plate and covered with 2.3 ml of 26% w/v Pluronic F-127 (Sigma-Aldrich) at 14 °C ...
-
bioRxiv - Plant Biology 2022Quote: ... Over the course of 12 hours they were gradually dehydrated to 95% ethanol and then stained overnight in 1% Acid Fuchsin (Sigma-Aldrich) in 95% ethanol ...
-
TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... 20 µl protein extract were separated on a 12% SDS gel and transferred to Nitrocellulose membrane (Sigma-Aldrich, St. Louis, MO). Anti-S6K1 (phospho T449 ...
-
bioRxiv - Microbiology 2022Quote: ... The cultures were harvested by centrifuging for 5 minutes at 12,000 rpm and resuspended in LB and transferred to wells of 12-well plate containing glass coverslips coated with polylysine (Sigma-Aldrich). The plate was incubated at 37°C for 48 hours after which the biofilm specimens were prepared for electron microscopy as described by Ochmcke et al ...
-
bioRxiv - Microbiology 2022Quote: ... cells seeded in 12-well plates were pre-treated with 500 mU/mL α2-3/6/8 sialidase from Clostridium perfringens (Sigma-Aldrich) for 3 hours at 37°C before infection ...
-
bioRxiv - Molecular Biology 2022Quote: ... human monocytic cell-lines into macrophages was performed by treatment with 30 nM phorbol-12-myristate-13-acetate (PMA; Sigma-Aldrich) for up to 24 hours ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were plated in 24-,12- or 6-well plates pre-coated with 10 μg/ml of poly-L-lysine (Sigma-Aldrich) and maintained at 37°C in a humidified atmosphere containing 5% CO2.
-
bioRxiv - Neuroscience 2023Quote: 12 - 24 week old mice were euthanized by intraperitoneal injection of urethane (8mg/g of body weight, ethyl carbamate, Sigma Aldrich) followed by decapitation ...
-
bioRxiv - Neuroscience 2022Quote: ... and cells were flushed from the column with 1□ml of prewarmed OPC medium (DMEM F/12 containing N-Acetyl cysteine (60 ug/ml, Sigma, #A9165), human recombinant insulin (10 ug/ml) ...
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were loaded into a 12% SDS-PAGE gels and transferred onto polyvinylidene difluoride membranes (pore size: 0.22 mm, Millipore, Billerica, USA). The membranes were blocked with 5% non-fat milk for 1 h and incubated with primary antibodies against β-actin (1:1000 ...
-
bioRxiv - Neuroscience 2022Quote: ... was carbon filtered and passed through PFA vials (Savillex 200-30-12) containing odor (2% methyl valerate in mineral oil, Sigma Aldrich product #1489977 ...
-
bioRxiv - Microbiology 2023Quote: U937-DC-SIGN cells were treated for 12 days with 160 nM Phorbol12-myristate-13-acetate (PMA) (Sigma-Aldrich®: P1585) and 30 ng/mL macrophage-colony stimulating factor (M-CSF ...
-
bioRxiv - Plant Biology 2023Quote: ... Total proteins were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride membranes (Merck Millipore, USA). OsJMT1-HA was detected by immunoblotting with HRP-conjugated anti-HA monoclonal antibodies (1:2,000 ...
-
bioRxiv - Neuroscience 2022Quote: Protein samples of RIPA-soluble or RIPA-insoluble/urea-soluble fractions were separated by electrophoresis (120 V for 90 min) on a polyacrylamide gel containing 12% acrylamide in the presence of reducing agent (2-mercaptoethanol, Sigma #63689). After SDS-PAGE ...
-
bioRxiv - Developmental Biology 2022Quote: ... were supplemented from day 15 to day 24 with a brief exposure (day 12–15) to retinoic acid (100 nM, Sigma-Aldrich). From day 25 to 42 ...
-
bioRxiv - Immunology 2022Quote: ... cells were stimulated with phorbol 12-myristate 13-acetate (PMA, 25 ng/mL) and ionomycin (750 ng/mL; both Sigma-Aldrich) in the presence of GolgiStop (4 μl/6mL ...
-
bioRxiv - Cell Biology 2024Quote: ... 1×105 ESCs were placed per well of a 12-well plate coated with 16.7 μg/mL bovine plasma fibronectin (Sigma-Aldrich, F1141) in N2B27 medium containing 20 ng/mL Activin A (Peprotech ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and non-transected controls were kindly provided by Novartis AG and were kept in standard culturing conditions (37 °C, 5% CO2) in ½ DMEM (Roth, 9005.1) – ½ F-12 (Sigma, N4888-500ml) media containing 10% FBS ...
-
bioRxiv - Molecular Biology 2023Quote: ... were separated in a 12% denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred to a polyvinylidene difluoride membrane (PVDF) (EMD Millipore) at 100V for 1h ...
-
bioRxiv - Developmental Biology 2024Quote: ... the lysates were separated on 8% v/v or 12% v/v polyacrylamide gels and transferred to a Polyvinylidene difluoride (PVDF) membrane (Millipore ISEQ00010). Membranes were incubated in 5% w/v milk buffer for 1 h at room temperature and incubated in primary antibody overnight at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... 12 mm (Marienfeld Superior; Cat Number: 633029) coated with 1 mg/mL poly-L-lysine (Sigma Chemical Co; Cat Number: P2636) at a density of 2000 cells/cm2 in minimum essential medium (MEM ...
-
bioRxiv - Molecular Biology 2024Quote: ... THP-1 monocyte differentiation was initiated by incubating the cells with 10 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma Aldrich) for 2 days at 37°C in a 5% CO2 environment ...
-
bioRxiv - Neuroscience 2024Quote: ... Two hundred thousand cells per well were seeded into a 12-well plate pre-coated with poly-D-lysine (5 μg/ml) in 0.1 M borate buffer (Sigma-Aldrich, #P7280). Cultures were grown in an incubator at 37°C and 5% CO2 ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were allowed to adhere to glass-bottom dishes for 12 hours and treated with freshly prepared CoCl2 (500 μM; Sigma-Aldrich) 12 hours prior to fixation ...
-
bioRxiv - Immunology 2024Quote: ... Thymi from 4-month-old to 12-year-old individuals were cryopreserved in liquid nitrogen in the following solution: 95% (PBS-5% Dextran 40 (Sigma-Aldrich)) – 5% DMSO (Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1×106 cells were seeded on 0.5 mg/ml poly-D-lysine-coated sterile 12-mm diameter coverslips or aligned 700-nm diameter nanofibers in 24 well plates (Sigma, Z694533). Growth medium was changed on days in vitro (DIV ...
-
bioRxiv - Cell Biology 2024Quote: ... THP-1 cells were plated to 24-well plate at a concentration of 2 × 105 viable cells per well and incubated for 48 h in 1 ml/well of media supplemented with 50 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich) for differentiation into macrophages ...
-
A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... Host cell lines included U937 monocytes (ATCC CRL-1593.2) which were differentiated into macrophages with 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich P1585) and A ...
-
A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... cells were plated on 24-well plates at 3x105 cells/well with 0.1 μg/mL 12-O-tetradecanoylphorbol-13-acetate (TPA, Sigma-Aldrich P1585) to promote differentiation ...
-
bioRxiv - Cancer Biology 2024Quote: ... Organs were minced with scissors and incubated in 10 ml digestion mix (DMEM/ F-12 containing 0.05% Collagenase D (Sigma-Aldrich, 11088858001), 0.3% Dispase II (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2024Quote: ... CFPAC1 and breast cancer cell line MCF7 were cultured in non-adherent system of ultra-low attachment vessel and serum-free DMEM/Nutrient Mixture F-12 medium (Sigma-Aldrich) supplemented with 2% B-27 ...
-
bioRxiv - Cell Biology 2024Quote: ... 106 THP-1 cells were transferred to each of the six-well plates and cultured with 100ng/mL phorbol 12-myristate 13-acetate (PMA) (P8139, Sigma-Aldrich) for 24h ...
-
bioRxiv - Neuroscience 2023Quote: ... demyelination was induced in the ventral white matter funiculus of the thoracic spinal cord (between vertebrae T11-12 or T12-13) by the injection of 1.2 µL of 1% (w/v) L-α-Lysophosphatidylcholine (Lysolecithin; Sigma-Aldrich) under general anaesthesia ...
-
bioRxiv - Microbiology 2023Quote: ... 100U/mL penicillin and 50 μg/mL streptomycin and differentiated into macrophages after treatment with 50 ng/mL phorbol-12-myristate-13-acetate (Sigma Aldrich) for 48 hours(42 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and loaded in 4–12% NuPAGE Bis-Tris gel (Fisher, 10247002) for subsequent electrophoresis and transference to nitrocellulose membrane (Merck-Sigma, GE10600003). Precipitated RNA was analyzed by autoradiography and FLAG-PTBP protein levels were measured by western blot.
-
bioRxiv - Developmental Biology 2023Quote: The anterior lobe of freshly dissected 8 to 12 week-old pituitaries was minced and incubated for 15mn at 37°C in 1mg/ml Papain (Sigma 10108014001), 10μg/ml DNAse I (Sigma 10104159001 ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: The riluzole was administered at a dose of ~12-15mg/kg/day p.o in 0.3% saccharin (Sigma Aldrich, St. Louis, MO) in drinking water ...
-
Neural circuit-wide analysis of gene expression during deafening-induced destabilization of birdsongbioRxiv - Neuroscience 2022Quote: ... Anhydrous 100% ethanol solution was prepared by adding 15 g of molecular sieve beads (Sigma 208582, 3 Å, 8-12 mesh) to 500 mL 100% molecular grade ethanol (Sigma E7023) ...
-
bioRxiv - Physiology 2023Quote: ... Conceptuses from day 16 were placed into Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham medium (DMEM/F12; Sigma - Aldrich, USA) supplemented with 1% (v/v ...
-
bioRxiv - Biochemistry 2023Quote: Reporter silencing assays were conducted as in ref 12 with the following changes: cells were treated with 1ug ml−1 dox (Sigma-Aldrich), and gates were set based on reference reporter cells cultured in parallel without dox and reference fully silenced reporter cells (See gating scheme in Figure S2).
-
bioRxiv - Systems Biology 2023Quote: ... The selected cells were differentiated into macrophages by stimulation in 20ng/mL phorbol 12-myristate 13-acetate (Sigma Aldrich, P8139-1mg) for 24 hours ...
-
bioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein per lane (20–50 μg) were loaded onto 4%–12% sodium dodecyl sulfate–polyacrylamide gels (Absin, China) and transferred to polyvinylidene difluoride membranes (Millipore, USA), and the membranes were incubated in 5% skim milk powder (weight/volume ...
-
bioRxiv - Neuroscience 2023Quote: ... and immediately homogenised in ice-cold homogenisation buffer (50 mM Tris-HCl pH 7.5, 100 mM KCl, 12 mM MgCl2, 1 mg/ml Heparin [Sigma-Aldrich], cOmplete EDTA-free protease inhibitors [Sigma-Aldrich] ...
-
bioRxiv - Physiology 2023Quote: THP-1 monocytes were differentiated into THP1-derived macrophages by stimulating 5×105 THP-1 monocytes/well in 6-well plate with 100 ng/mL phorbol 12-myristate 13-acetate (PMA) (#P8139, Sigma Aldrich) for 48 h ...
-
bioRxiv - Cell Biology 2023Quote: ... cell population potentially harboring latent virus was sorted and stimulated with 10 ng/mL Phorbol 12-Myristate 13-Acetate (PMA, Sigma Aldrich) followed by sorting the GFP (+ ...
-
bioRxiv - Molecular Biology 2023Quote: ... cells were grown to 70–80% confluency (optionally with 1 μg/ml doxycycline for 24–48 h) in 24- or 12-well plates and treated with colchicine (10 μM, Sigma PRH1764), combretastatin A4 (Selleckchem S7783) ...