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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 500,000 HCT-116 cells were plated in 12-well plates in 1 mL complete medium containing lentiviruses supplemented with 8 µg/mL polybrene (Sigma H9268) and cells were spun down at 950 x g for 90 min at 30°C ...
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bioRxiv - Immunology 2024Quote: ... Duck fibroblast cell lines were cultured in 5% CO2 atmosphere at 37°C in medium containing mixture of 1:1 (DMEM)/F-12 (Sigma-Aldrich) supplemented with 4% calf serum (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... the cells were stimulated with 500 nM ionomycin and 10 ng/ml phorbol-12-myristate-13-acetate (PMA) (both from Merck Sigma-Aldrich), and BD GolgiPlug Protein Transport Inhibitor (containing Brefeldin A ...
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bioRxiv - Immunology 2024Quote: ... THP-1 monocytes are differentiated into macrophages by 24 h incubation with 150 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139) followed by 24-48 h incubation in fresh RPMI medium ...
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bioRxiv - Immunology 2024Quote: For ex vivo stimulation one third of Percoll-isolated brain leukocytes were incubated for 4h at 37°C with Phorbol 12-Myristate 13-Acetate (PMA) (50 ng/ml) and ionomycin (1000 ng/ml) (Sigma-Aldrich) in presence of monensin (Golgistop ...
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bioRxiv - Immunology 2024Quote: ... THP1 monocytes were differentiated to macrophages after 3-h exposure to 50 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma–Aldrich) in 1 mL of growth medium ...
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bioRxiv - Immunology 2024Quote: ... 10 μg/ml of brefeldin A and 0.05 μg/ml of phorbol 12-myristate 13-acetate (Sigma Aldrich Millipore, Oakville, Canada) for four-hours at 37 □C at 5% CO2 ...
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bioRxiv - Immunology 2024Quote: ... 10 μg/ml of brefeldin A and 0.05 μg/ml of phorbol 12-myristate 13-acetate (Sigma Aldrich Millipore, Oakville, Canada) for four-hours at 37 □C at 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... of the three cell lines were separated in a 12% denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred to a polyvinylidene difluoride membrane (PVDF) (EMD Millipore) at 100V for 1h ...
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bioRxiv - Microbiology 2024Quote: ... Nuclei Lysis Buffer was prepared by adding 12 mL of Nuclei EZ Prep Lysis Buffer (Sigma-Aldrich, St. Louis, MO, USA) to a 15mL tube and adding 1 cOmplete Ultra tablet (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... THP1-b monocytes were previously induced to differentiate into macrophages using 150 nM PMA (Phorbol 12-myristate 13-acetate; Sigma, P8139). Samples were fixed using Pierce 4% PFA (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... were seeded in 6-well plates (1.5×106 cells/well) with or without phorbol 12-myristate 13-acetate (PMA, 100 ng/ml, 2 ml/well, Sigma-Aldrich) and incubated at 37°C for 48 hrs ...
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bioRxiv - Microbiology 2024Quote: ... Equivalent amounts of total protein were loaded into a 12% SDS-PAGE for electrophoresis and then transferred into a PVDF membrane (Millipore, USA). After blocking nonspecific binding with 5% skimmed milk in TBST ...
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bioRxiv - Neuroscience 2024Quote: ... on PEI/Laminin-coated surfaces at 55,000-60,000 cells/cm2 (200k/well of a 12 well) in N2/B27 medium supplemented with 1 mg/mL doxycycline (Sigma-Aldrich), 10 ng/mL BDNF (R&D systems) ...
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bioRxiv - Physiology 2024Quote: ... FSUs were cultured in 2 mL Dulbecco’s Modified Eagle Medium/Nutrient Mixture F-12 Ham with L-glutamine and 15 mM HEPES (Sigma-Aldrich, D6421). Culture medium was supplemented with 20% fetal bovine serum (Gibco No ...
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bioRxiv - Molecular Biology 2024Quote: ... 20 μL of each sample was loaded onto a 12% SDS-PAGE gel for electrophoresis and transferred to Immobilon-FL PVDF membrane (Millipore, IPFL00010) using a Power-Blotter Semi-dry transfer system (ThermoFisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 30 μg of protein from each sample were electrophoresed on a 4-12% SDS gradient gels and transferred to polyvinylidene difluoride membranes (Nitrocellulose; Millipore Corp). 1% BSA was used as blocking buffer for all phosphate proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were followed by 12 h culture in the presence of microtubule-disrupting agent nocodazole at 100 µM concentration (Sigma-Aldrich). To remove nocodazole ...
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bioRxiv - Bioengineering 2024Quote: ... 400k cells were plated the evening prior in a 12 cm dish that had 18 mM coverslips precoated with poly-L-lysine (Sigma-Aldrich #P7886 ...
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bioRxiv - Cell Biology 2024Quote: ... T cells were re-stimulated using a combination of phorbol 12-myristate 13-acetate (PMA) (50ng/ml) (Sigma-Aldrich, Cat: P8139) and Ionomycin (1μg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... explants (n = 7-12/group) were washed for 2 hours and then digested with proteinase K (5 mg/mL) (Sigma-Aldrich) for 16 hours ...
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bioRxiv - Bioengineering 2024Quote: ... cells were seeded at 0.5 × 106 cells mL-1 in 75 cm2 tissue culture flasks and stimulated with 50 ng mL-1 of PMA (phorbol 12-myristate 13-acetate; Sigma-Aldrich). After 24 h ...
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bioRxiv - Cell Biology 2024Quote: H9c2 cells were seeded onto fibronectin coated coverslips either purchased from Neuvitro (NEU-GG-12-1.5-FIBRONECTIN) or coated in lab using fibronectin bovine plasma (Sigma, F1141-1MG). Coverslips were fixed with 4% paraformaldehyde for 5 minutes at DIV0 ...
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bioRxiv - Genetics 2024Quote: ... in TC-treated 12- or 6-well plates coated with 0.05 mg/mL Poly-D-lysine (Sigma-Aldrich A-003-E). At 3-4 DIV ...
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bioRxiv - Developmental Biology 2024Quote: ... Sperm was collected from the vas deferens and caudal epididymis at 12 and 39 weeks-of-age and capacitated in EmbryoMax Human Tubal Fluid medium (HTF, EMD Millipore) with 3% w/v bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated with antibodies (anti-Pol2, Abcam ab5408; anti-H3, Sigma H9289; anti-H3K4me3, Abcam ab8580; anti-H3K9me2, Diagenode C15410060; anti-H3K27me3, Millipore 07-449) at 4°C for 2 hours ...
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bioRxiv - Bioengineering 2020Quote: ... Phosphate buffered saline powder (PBS, D5652), collagenase from Clostridium histolyticum (Type I, C0130) and Triton-X 100 (T8787) were purchased from Sigma-Aldrich (St Louis, MO, USA). Foetal bovine serum (35010CV ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... we mixed 500 mL tap water with 38 g gelatin from porcine skin (gel strength 300, Type A, G2500, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany), 25 g honey (Gut & Günstig Bienenhonig flüssig ...
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bioRxiv - Genetics 2020Quote: Exopolyphosphatase activity of wild-type PRUNE1 on medium and long chain PolyP substrates (consisting of 45, 65 and 700 orthophosphate monomers, Sigma S4379, Sigma S6253, Kerafast EUI002) was assessed by comparing to S ...
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bioRxiv - Microbiology 2021Quote: ... ovatus cells were grown to mid-exponential phase on either purified mucin O-linked glycans (purified in house from Sigma Type III porcine gastric mucin) or glucose as a reference as previously described (22) ...
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bioRxiv - Neuroscience 2023Quote: ... minced using sterile razor blades and digested in a 5 mL tube containing 3-5 mL of the digestion mix at 37 °C for 90 minutes: Collagenase type V (Sigma-Aldrich C9263; 5 mg/mL) and Dispase II (Gibco 17105041 ...
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bioRxiv - Bioengineering 2023Quote: ... 5 g of powdered gelatin sourced from porcine skin (Type A, with a Bloom strength of 300, obtained from Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 50 mL of RO purified water ...
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bioRxiv - Microbiology 2023Quote: ... with the exception of Akkermansia muciniphila DSMZ 22959 that was pre-cultured in M2-based medium supplemented with 3 g/L of type II mucin (Sigma-Aldrich Chemie GmbH, Buchs, Switzerland), and Veillonella parvula DSMZ 2008 that was pre-cultured in M2-based medium supplemented with 60 mM of DL-lactic acid 90% (Sigma-Aldrich Chemie GmbH ...
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bioRxiv - Neuroscience 2023Quote: HUVECs transfected with RNF213 wild-type (WT) or R4810K under OGD conditions were fixed in 2% glutaraldehyde solution (15720, Sigma-Aldrich, Saint Louis, MO, USA). After fixation ...
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bioRxiv - Molecular Biology 2022Quote: ... The DNA samples were loaded to 0.49 ug and run on a 1.1% agarose gel (Sigma-Aldrich Type I-A, low EEO catalog no. A0169) in 1x Tris/acetic acid/EDTA (TAE ...
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bioRxiv - Developmental Biology 2023Quote: ... final concentration 20 μM in Figure3 d-e and 40μM in Figure3 g-h) or Collagenase type IV (Sigma C5138, final concentration 20 μg/ml). Control explants were cultured with BME supplemented with the drug vehicle ...
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bioRxiv - Bioengineering 2024Quote: ... the outlined procedure involves the dissolution of 5g of porcine skin-derived powdered gelatin (Type A, Bloom strength 300, Sigma-Aldrich, St. Louis, MO, USA) in 50 mL of distilled water ...
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bioRxiv - Cancer Biology 2024Quote: ... G12D or wild-type Scgb1a1-CreERTM(WT) littermates were administered five consecutive daily intraperitoneal (IP) injections of 20 mg/ml tamoxifen (Sigma-Aldrich, St. Louis, MO, USA) dissolved in corn oil at a dose of 1 mg tamoxifen/10 g body weight ...
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bioRxiv - Physiology 2020Quote: ... anti-Actin and anti-Tubulin (Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... anti-HA and anti-VSV-G (Sigma cat ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% v/v Anti-anti (Sigma, USA), 2% v/v Gem21 NeuroPlex™ Serum-Free (without Vitamin A ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
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bioRxiv - Cell Biology 2020Quote: ... transfected HeLa cells were grown overnight on 12-well glass slides at 37°C and treated with 250 nM dexamethasone (Dex) (Sigma-Aldrich, D4902) to induce RGG nuclear import for the indicated times ...
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bioRxiv - Molecular Biology 2021Quote: ... 150K cells were plated in 24-well flat bottom plates on coverslips (12 mm, thickness ~0.1 mm) coated with Poly-D-Lysine (Sigma-Aldrich P7886-50MGP) in Minimum essential medium (MEM) ...
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bioRxiv - Genomics 2020Quote: ... cells were grown to a density of 8.0 × 105 cells/mL and treated with 25ng/mL Phorbol 12-Myristate 13-Acetate (PMA) (Sigma-Aldrich, Catalogue #P8139) for 4 days ...
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bioRxiv - Immunology 2021Quote: Degranulation and cytokine production of NK cell subsets were determined after cytokine stimulation o/n with IL-12 (10ng/ml, Sigma Aldrich, USA) and IL-18 (5ng/ml ...
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bioRxiv - Developmental Biology 2021Quote: ... From day 6 until day 12 the medium was changed once in two days with the addition of 1 mM valproic acid (Sigma-Aldrich, USA). On day 23 colonies were picked up and expanded on the feeder ...
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bioRxiv - Bioengineering 2021Quote: ... Total cell lysate for each sample were resolved on 12% SDS-PAGE gels and transferred to polyvinylidene fluoride membranes (Millipore, Billerica, MA). The membranes were blocked in LI-COR blocking buffer (LI-COR ...
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bioRxiv - Neuroscience 2021Quote: ... for 12 h at 4°C after blocking for 30 min at room temperature with 5% donkey serum (S30-100ml, Millipore, MA, USA). Subsequently ...
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bioRxiv - Biochemistry 2021Quote: ... Eluate was resuspended in laemmlil and run directly on a 12% polyacrylamide gel and immunoblotted using 1:1000 M2-FLAG antibody (Sigma; cat. F1804).