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Citations for Millipore Sigma :
2601 - 2650 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2020Quote: ... after 12 h fasting by a single intraperitoneal (i.p.) injection of streptozotocin (STZ) (Sigma Chemical Co., St, Louis, MO, USA) at a dosage of 60mg/Kg of body weight (BW ...
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bioRxiv - Systems Biology 2020Quote: ... Total proteins from diverse organelles were extracted using a phase-transfer surfactant solution containing 12 mM sodium deoxycholate (Sigma–Aldrich), 12 mM sodium lauroyl sarcosine (MP Biomedical ...
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bioRxiv - Physiology 2020Quote: ... and then cut into pieces (~1 mm in diameter) using a sterile scalpel before being placed evenly in 12-well tissue culture plates in αMEM medium (Sigma) containing 2% FCS [32].
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bioRxiv - Immunology 2020Quote: ... or 5 (mice) hours of culture with phorbol 12-myristate 13-acetate (50□g/mL) and ionomyocin (500ng/mL, Sigma) in the presence of Brefeldin A ...
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bioRxiv - Molecular Biology 2020Quote: 25 mm round cover glass (Fisher Brand, #12-545-102 25CIR-1) was coated with 3-Triethoxysilypropylamine APES (Millipore, #A3648) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... medium was replaced with fresh DMEM/F-12 2% FBS containing 8µg/ml of polybrene (hexadimethrine bromide; Sigma-Aldrich, # H9268). Control wells were left without treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... U937 cells were cultured in RPMI1640 media and induced by 5nM PMA (phorbol 12-myristate-13-acetate) (P1585, Sigma-Aldrich) for 48 hours as per protocol(32 ...
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bioRxiv - Synthetic Biology 2020Quote: Full-thickness porcine articular chondrocytes were enzymatically isolated from the femurs of pigs (∼30 kg; 12-16 weeks old) using collagenase (Sigma) media ...
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bioRxiv - Pathology 2021Quote: ... rats in the high-dose 7-d L-DOPA priming group (n=6) were treated daily with L-DOPA (12 mg/kg) + benserazide (15 mg/kg, s.c., Sigma-Aldrich) for 7 d (i.e. ...
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bioRxiv - Microbiology 2020Quote: Caco-2 cells were seeded at 2 × 105 cells/well onto polycarbonate Millicell culture-plate inserts (12-mm diameter, 3-μm pore size; Millipore) and cultured for 5 days ...
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bioRxiv - Immunology 2021Quote: 4D6 cells were plated into 12 well cell culture plates containing sterile coverslips coated with lysine (1:10 dilution in PBS, Sigma) and transfected as described earlier ...
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bioRxiv - Immunology 2021Quote: ... embedded in OCT (Sakura) and cut to 12 μm sections that were stained with Luxol Fast Blue (LFB) stain (Sigma). Mosaic images were acquired using a Zeiss Axio Observer.Z1-Inverted Microscope with Apotome ...
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bioRxiv - Genetics 2021Quote: ... 0.2×106 mESCs were seeded per 12-well and transduced the next day with 500 µl of viral supernatant and 8 ng/µl polybrene (Sigma). Antibiotic selection was started two days after transduction and kept for at least 3 passages.
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bioRxiv - Genetics 2020Quote: ... Trolox was made by using 12 mM Trolox (6Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid, Sigma-Aldrich, MO, USA; 238813–1G) dissolved in 12 mM NaOH ...
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bioRxiv - Neuroscience 2020Quote: ... and 10 μl of internal standard (IS; containing 12 pmol of D3-morphine and 10.5 pmol of D3-M3G; Sigma Aldrich) and 100 μl of ice-cold acetonitrile (ACN ...
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bioRxiv - Biochemistry 2021Quote: ... Experimental molecular weights were calculated using calibration standards from the Gel Filtration Molecular Weight Markers Kit for molecular weights 12-200 kDa (Sigma) and the Gel Filtration Calibration Kit for molecular weights 43-669 kDa (Cytiva) ...
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bioRxiv - Neuroscience 2021Quote: ... or by either bath application or a custom-made perfusion system (for imaging cells cultured on 12-mm coverslips): ATP (Sigma), ADP (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... The complete growth medium comprised Dulbecco’s modified Eagle medium with nutrient mixture F-12 (DMEM/F12) supplemented with 33 mM D-glucose (Sigma G8644), 19 mM NaHCO3 (Sigma S8761) ...
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bioRxiv - Immunology 2021Quote: 293T Lenti-X cells (2.5×104/well) were seeded in 24-well microplates onto 12-mm cover glasses previously treated with L-polylysine (Sigma) and transiently transfected with pGAE-UFO.664 ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli K-12 (ATCC 19020) and Escherichia coli EK-19 were inoculated in sterile Luria Broth (LB, L3522, Sigma) and incubated at 37 °C overnight with a shaking speed of 200 rpm ...
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bioRxiv - Immunology 2021Quote: ... OAS1 KO 293T cells were seeded in 12 well plates coated with 10 μg/mL poly-L-ornithine hydrobromide (Sigma) and allowed to adhere overnight ...
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bioRxiv - Immunology 2021Quote: ... cells were stimulated with Brefeldin A at 10µg/ml alone or in combination with Phorbol-12-myristate-13-acetate (PMA) (Sigma-Aldrich) and ionomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... and differentiated at 37°C in 5% CO2 using 20 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma, P8139) for 24 h ...
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bioRxiv - Immunology 2021Quote: Cells harvested from in vitro culture or in vivo mice experiments were stimulated with 50 ng/mL PMA (Phorbol 12-myristate 13-acetate, Sigma) and 1 µM ionomycin (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Cells were resuspended in RPMI containing 5% FBS and re-stimulated with 50 ng/ml phorbol 12-myristate 13-acetate (PMA, Sigma) plus 1 μg/ml ionomycin (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins from whole cell lysates (20–60 µg) were separated on 8-10-12% polyacrylamide gels and transferred to Immobilon-FL membranes (Millipore). Primary and secondary antibodies were diluted in blocking buffer (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plates were read using a Mithras LB 940 multimode microplate reader (Berthold Technologies) and 100 μM phorbol 12-myristate 13-acetate (PMA, Sigma) used as a positive control ...
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bioRxiv - Immunology 2021Quote: ... total mononuclear cells isolated from the epithelium were plated in 48-well plates and incubated at 37° C with 100ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma), 200ng/mL ionomycin (Sigma ...
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ATM-mediated DNA damage response in macrophages primes phagocytosis and immune checkpoint regulationbioRxiv - Immunology 2020Quote: ... of 106 cells/ml/well in a 12-well plate with fresh media or with fresh media supplemented with 1000 ng/ml LPS (Sigma) for 6 hrs ...
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bioRxiv - Neuroscience 2022Quote: ... 25.000 NSPC per well were seeded into 12-mm coverslips coated with laminin (L2020, Sigma-Aldrich, St Louis, MO, USA) as previously described (Silvestre et al. ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant containing dissociated cortical neurons was seeded on 12 or 24 well plates containing coverslips (Neuvitro Corporation) precoated overnight with 50 µg/ml poly-D-lysine (Sigma). Twelve-well plates contained 18 mm coverslips seeded at a density of 200’000 cells per well and 24 well plates contained 12 mm coverslips seeded at a density of 100’000 cells per well ...
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bioRxiv - Cancer Biology 2022Quote: ... metformin was placed in the drinking water of mice at 1.25 mg/ml for 12 weeks prior to sacrifice (Sigma-Aldrich). No statistical methods were used to predetermine sample sizes ...
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bioRxiv - Cancer Biology 2022Quote: ... As positive control T cells have been non-specifically stimulated with 0.5 ng/µL phorbol-12-myristate-13- acetate (PMA, Sigma Aldrich) and 1 ng/µL Ionomycin (Sigma Aldrich) ...
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bioRxiv - Biophysics 2022Quote: ... 10 μM of biotin-labeled tubulin and 3.5 μM of Alexa647-labelled microtubules tubulin were incubated with 2 mM of GTP and 12 mg/ml CPA (Sigma) for 20 min at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... The next day the GelMA solution was dialysed using dialysis with molecular weight cut-off of 12 – 14kDa (Sigma-Aldrich) for up to 5 days against deionised water pH 9.0 ...
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bioRxiv - Cell Biology 2022Quote: ... Approximately 100 mg fragments were incubated in a 12-well plate with M199 media ± 7 nM insulin (Actrapid, Novo Nordisk) and 25 nM dexamethasone (D4902, Sigma). After 24 hr incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 μg of protein per sample was loaded and separated on 8-12% SDS–PAGE gels and electro blotted onto polyvinylidenedifluoride (PVDF) membranes (Immobilon-P PVDF membrane 0.45μm; Merck-Millipore, Burlington, USA). Subsequently ...
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bioRxiv - Genomics 2022Quote: ... followed by culture on 0.1% gelatin-treated plates for 1 week in hepato-medium (DMEM:F-12, supplemented with 10% FBS, 1 mg/ml insulin (Sigma-Aldrich), dexamethasone (Sigma-Aldrich) ...
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bioRxiv - Genetics 2022Quote: ... 0.2 × 106 mESCs were seeded per 12-well and transduced the next day with 50 μl of concentrated viral supernatant and 8 ng/μl polybrene (Sigma). Antibiotic selection was started 2 days after transduction and kept until all cells in the non-transduced control were dead (typically within 1-2 passages).
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bioRxiv - Molecular Biology 2022Quote: ... cells were seeded into 12-well tissue culture trays at a density of 2×105 cells per well and pretreated with 10-8 M phorbol 12-myristate 13-acetate (PMA) (Sigma) for 72 h ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then separated on 10-12% polyacrylamide gels and transferred onto methanol-activated polyvinylidene difluoride (PVDF) Hybond-P membranes (Millipore). Membranes were blocked in Tris-buffered saline with 0.1% Tween-20 (TBS-T ...
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bioRxiv - Immunology 2022Quote: ... cell suspensions were stimulated 4 h at 37°C with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) (Sigma), 1 μg/mL ionomycin (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... 3×105 cells were seeded into 12-well plates that had been coated with 1 mg/mL poly-L-ornithine (Sigma) for 60 minutes ...
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bioRxiv - Molecular Biology 2022Quote: 15-30 μg of protein extract were resolved on an 8% or 12% polyacrylamide-SDS gel and transferred to a polyvinylidene difluoride membrane (PVDF, Millipore). Primary antibodies were used at the recommended dilutions in 5% milk or 3% BSA diluted in TBS-Tween ...
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bioRxiv - Cancer Biology 2022Quote: ... Cancer cells at 22,000 cells/ml and PS-1 cells at 44,000 cells/ml were combined with DMEM/F-12 and 1.2% methylcellulose in a 4:1 ratio of methylcellulose (Sigma-Aldrich) and 20 μl drops ...
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bioRxiv - Immunology 2022Quote: ... T cell subsets were rested overnight at 37°C then stimulated for 6 h with phorbol 12-myristate 13-acetate (25 ng/mL; Sigma) and ionomycin (1 mg/mL ...
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bioRxiv - Cancer Biology 2024Quote: 3T3-L1 cells (70,000 cells/well - 12 well plate) were grown to 100% confluency in preadipocyte Growth media (High glucose DMEM (Sigma D5796); 10% Newborn Calf Serum (Sigma 12023C) ...
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bioRxiv - Physiology 2024Quote: ... and maintained at 37 °C with 5% CO2 in a 1:1 mixture of DMEM and Ham’s F-12 medium (D 8900, Sigma-Aldrich), supplemented with 50 ng/ml mouse Nerve Growth Factor-7S (NGF ...
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bioRxiv - Neuroscience 2024Quote: ... and reseeded at lower density in 12 mm diameter glass coverslips previously treated with poly-L lysine (0.01%, Sigma-Aldrich).
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bioRxiv - Plant Biology 2024Quote: ... the suspension was transferred to dialysis tubing with a 12-kDa MW cut-off (32 mm flat width; Sigma, USA) and stirred overnight ...