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Citations for Millipore Sigma :
2901 - 2950 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... were separated in a 12% denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and electrotransferred to a polyvinylidene difluoride membrane (PVDF) (EMD Millipore) at 100V for 1h ...
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bioRxiv - Developmental Biology 2024Quote: ... the lysates were separated on 8% v/v or 12% v/v polyacrylamide gels and transferred to a Polyvinylidene difluoride (PVDF) membrane (Millipore ISEQ00010). Membranes were incubated in 5% w/v milk buffer for 1 h at room temperature and incubated in primary antibody overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Then 10 mg of protein per sample was separated on 12% SDS-PAGE gels (Sangon Biotech) and subsequently transferred to PVDF membrane (Millipore, Temecula) using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques ...
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bioRxiv - Microbiology 2023Quote: ... 5 × 105 cells/mL were seeded in the wells of flat-bottom tissue culture plates and matured into macrophages with 20 nM phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich) during 72 h ...
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bioRxiv - Microbiology 2023Quote: Cells were treated as indicated in the figures or figure legends with the following compounds: PMA (phorbol 12-myristate 13-acetate, Sigma Aldrich), Valproic acid (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were cultured in Poly-L-Ornitine pre-coated flat bottom 12-well plate in a high glucose DMEM (Sigma, D7777) medium supplemented with 44 mM NaHCO3 (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... 12 h post-transfection the media was removed and fresh media was added with 3 mM Sodium butyrate (Sigma-Aldrich, USA) to induce lentivirus replication ...
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bioRxiv - Neuroscience 2023Quote: ... 12 mm (Marienfeld Superior; Cat Number: 633029) coated with 1 mg/mL poly-L-lysine (Sigma Chemical Co; Cat Number: P2636) at a density of 2000 cells/cm2 in minimum essential medium (MEM ...
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bioRxiv - Neuroscience 2023Quote: ... demyelination was induced in the ventral white matter funiculus of the thoracic spinal cord (between vertebrae T11-12 or T12-13) by the injection of 1.2 µL of 1% (w/v) L-α-Lysophosphatidylcholine (Lysolecithin; Sigma-Aldrich) under general anaesthesia ...
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bioRxiv - Immunology 2022Quote: ... Macrophages were generated by differentiating THP-1 monocytes with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) (Millipore Sigma, P8139) for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... for approximately 12-18 hours and then subjected to shear stress (130rpm) in new medium containing 4% dextran (Sigma-Aldrich #31392) for indicated time intervals and cultured alongside static controls ...
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bioRxiv - Bioengineering 2022Quote: ... ATCC® CRL-4003) were maintained as per the manufacturer’s instructions in custom phenol red-free DMEM/F-12 (based on Sigma #D 2906) supplemented with 1% ITS+ Premix (Corning 354352) ...
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bioRxiv - Biophysics 2022Quote: ... NaCl and indium titanium oxide (ITO) coated glass slides (surface resistivity 8–12 V sq-1) were purchased from Sigma-Aldrich Co ...
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bioRxiv - Cell Biology 2022Quote: ... Dissociated cells from one embryonic heart were plated on one glass coverslips (diameter 12 mm) pre-coated with bovine fibronectin (10 μg/ml in PBS; Sigma-Aldrich) in 50 μl DMEM/10% FCS/penicillin/streptomycin at 37 °C in a humidified atmosphere of 95% air and 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... T cells were stimulated with 50 ng/ml phorbol 12-myristate 13-acetate (PMA) and 500 ng/ml ionomycin (Sigma-Aldrich) in the presence of GolgiStop (BD ...
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bioRxiv - Immunology 2022Quote: ... THP-1 monocytes are differentiated into macrophages by 36 h incubation with 20 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139). The murine macrophage Raw 274.7 cells (ATCC ...
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bioRxiv - Genetics 2022Quote: Female XEN cell line was grown on 0.2% gelatin-coated flasks following the Rossant lab XEN stem cell protocol (https://lab.research.sickkids.ca/rossant/wp-content/uploads/sites/12/2015/08/XEN-Stem-Cell-protocol1.pdf) in serum-containing XEN medium (RPMI 1640 (Sigma, M3817)) ...
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bioRxiv - Genetics 2022Quote: ... 30 adult worms were dropped in 10 μl S-basal in a well of a Corning Costar® 12-well plate and covered with 2.3 ml of 26% w/v Pluronic F-127 (Sigma-Aldrich) at 14 °C ...
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bioRxiv - Plant Biology 2022Quote: ... Over the course of 12 hours they were gradually dehydrated to 95% ethanol and then stained overnight in 1% Acid Fuchsin (Sigma-Aldrich) in 95% ethanol ...
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TOR acts as metabolic gatekeeper for auxin-dependent lateral root initiation in Arabidopsis thalianabioRxiv - Plant Biology 2022Quote: ... 20 µl protein extract were separated on a 12% SDS gel and transferred to Nitrocellulose membrane (Sigma-Aldrich, St. Louis, MO). Anti-S6K1 (phospho T449 ...
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bioRxiv - Microbiology 2022Quote: ... The cultures were harvested by centrifuging for 5 minutes at 12,000 rpm and resuspended in LB and transferred to wells of 12-well plate containing glass coverslips coated with polylysine (Sigma-Aldrich). The plate was incubated at 37°C for 48 hours after which the biofilm specimens were prepared for electron microscopy as described by Ochmcke et al ...
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bioRxiv - Microbiology 2022Quote: ... cells seeded in 12-well plates were pre-treated with 500 mU/mL α2-3/6/8 sialidase from Clostridium perfringens (Sigma-Aldrich) for 3 hours at 37°C before infection ...
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bioRxiv - Molecular Biology 2022Quote: ... human monocytic cell-lines into macrophages was performed by treatment with 30 nM phorbol-12-myristate-13-acetate (PMA; Sigma-Aldrich) for up to 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated in 24-,12- or 6-well plates pre-coated with 10 μg/ml of poly-L-lysine (Sigma-Aldrich) and maintained at 37°C in a humidified atmosphere containing 5% CO2.
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bioRxiv - Neuroscience 2023Quote: 12 - 24 week old mice were euthanized by intraperitoneal injection of urethane (8mg/g of body weight, ethyl carbamate, Sigma Aldrich) followed by decapitation ...
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bioRxiv - Neuroscience 2022Quote: ... and cells were flushed from the column with 1□ml of prewarmed OPC medium (DMEM F/12 containing N-Acetyl cysteine (60 ug/ml, Sigma, #A9165), human recombinant insulin (10 ug/ml) ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were loaded into a 12% SDS-PAGE gels and transferred onto polyvinylidene difluoride membranes (pore size: 0.22 mm, Millipore, Billerica, USA). The membranes were blocked with 5% non-fat milk for 1 h and incubated with primary antibodies against β-actin (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... was carbon filtered and passed through PFA vials (Savillex 200-30-12) containing odor (2% methyl valerate in mineral oil, Sigma Aldrich product #1489977 ...
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bioRxiv - Microbiology 2023Quote: U937-DC-SIGN cells were treated for 12 days with 160 nM Phorbol12-myristate-13-acetate (PMA) (Sigma-Aldrich®: P1585) and 30 ng/mL macrophage-colony stimulating factor (M-CSF ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteins were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to polyvinylidene fluoride membranes (Merck Millipore, USA). OsJMT1-HA was detected by immunoblotting with HRP-conjugated anti-HA monoclonal antibodies (1:2,000 ...
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bioRxiv - Immunology 2023Quote: ... lymph nodes and splenic cell suspensions were stimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich, #P8139), 1 µM Ionomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... THP-1 cells were differentiated into phenotypically adherent macrophages by stimulation with 100 nM Phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) in RPMI complete ...
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bioRxiv - Immunology 2022Quote: ... cells were first restimulated in vitro with 50ng/mL phorbol 12-myristate 13-acetate (PMA) + 2 μg/mL ionomycin (both Sigma-Aldrich) in the presence of Monensin (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... Both ex vivo and IL-25-stimulated cells were cultured in complete RPMI medium in the presence of phorbol-12-myristate-13-acetate (500 ng/ml) (Sigma-Aldrich) and ionomycin (250 ng/ml ...
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bioRxiv - Neuroscience 2023Quote: ... of the rostral-dorsal striatal wall in newborn male and female mice was dissected out and mechanically dissociated in DMEM/F-12 containing 100 units/mL penicillin‒streptomycin (P0781, Sigma‒Aldrich) to obtain NSCs ...
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bioRxiv - Cancer Biology 2023Quote: Decellularized matrices were fixed for 30 minutes under gentle agitation (12 rpm orbital 154 shaker) in 3 % glutaraldehyde solution in cacodylate buffer (100 mM, Sigma 20840) at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... equal amounts of proteins were loaded onto polyacrylamide gels (8-12%) under reducing conditions and transferred to Immobilon-P membranes (EMD Millipore). After blocking with 5% BSA (wt/vol ...
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bioRxiv - Cell Biology 2024Quote: Organoids collected between day 16 to day 21 after replating (between passage 5 and 12) were washed with PBS and fixed with ice-cold glutaraldehyde 2% (Electron microscopy grade, Sigma #G5882) and post-fixed in 2% OsO4 in 0.1M cacodylate buffer (pH 7.2) ...
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bioRxiv - Immunology 2024Quote: ... single-cell suspensions were stimulated for 3.5 hours at 37°C with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 mM ionomycin (both from Sigma-Aldrich) in the presence of protein transport inhibitor cocktail (eBioscience ...
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bioRxiv - Immunology 2024Quote: ... individual lung lobes were isolated and homogenized in 1 mL PBS and subsequently hydrolyzed through the addition of 1 mL of 12 N HCl (Sigma Aldrich) followed by incubation at 120°C for 18 hours ...
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bioRxiv - Cell Biology 2024Quote: ... WIS) in hESb medium composed of Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham (DMEM/F12, Sigma #D8437), 20 % KO serum replacement (KOSR ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 μg of protein lysate was electrophoresed on 12% SDS-polyacrylamide gels and transferred to PVDF membranes (EMD Millipore, Burlington, MA). After blocking with 5% milk ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 30 μg of protein was separated on a 12% polyacrylamide gel and transferred onto a PVDF membrane (Immobilon-P, Millipore, IPVH00010) in a 20 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... and THP-1 (ATCC® TIB-202) human monocytic cells into macrophages was performed by treatment with 3 nM phorbol-12-myristate-13-acetate (PMA; Sigma-Aldrich) for up to 24 hours ...
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bioRxiv - Neuroscience 2024Quote: HEK 293T cells in 12-well plates were transfected with 1 g of plasmid DNA using Polyethylenimine PEI (1 mg/ml, Sigma, 408727) at a 1:3 w/v ratio ...
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bioRxiv - Cell Biology 2024Quote: ∼106 vegetative (growth stage) amoebae were seeded onto 12-mm diameter glass coverslips coated with poly-L-lysine (1 μg/ml, Sigma Aldrich) and allowed to adhere for 30 min at 22°C ...
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bioRxiv - Microbiology 2024Quote: THP1 (ATCC TIB-202) monocytes (4.75x105 cells/mL) differentiated into macrophages in phorbol 12-myristate 13-acetate (PMA, 20 ng/ml; Sigma-Aldrich, P1585) were infected with culture of bacteria at an OD600nm of 0.5 at MOI 100 ...
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bioRxiv - Microbiology 2024Quote: ... THP1 were differentiated for 48 hrs in macrophage media supplemented with 50 ng/mL Phorbol 12-myristate 13-acetate (in DMSO, Sigma-Aldrich). PMA-containing media was replaced with fresh macrophage media and cells were rested overnight prior to infection.
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bioRxiv - Microbiology 2024Quote: ... THP-1 cells were differentiated into macrophage-like cells by treatment with 100LJng/ml phorbol 12-myristate 13-acetate PMA (Sigma-Aldrich). Overnight bacterial growths were diluted 1:100 in BHI medium and grown until an OD600nm ≈ 1 ...
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bioRxiv - Microbiology 2024Quote: ... THP-1 monocyte differentiation was initiated by incubating the cells with 10 ng/mL phorbol 12-myristate 13- acetate (Sigma-Aldrich) for 2 days at 37°C in a 5% CO2 environment ...