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Citations for Millipore Sigma :
2801 - 2850 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... and minced in growth media (DMEM Ham’s F-12, 10% FBS, 1X N2 or N1 supplement and 16 nM Cytosine arabinoside (Sigma-Aldrich, C1768) with McPherson-Vannas Micro Dissecting Spring scissors ...
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bioRxiv - Immunology 2021Quote: ... cells were either left unstimulated or activated for 4 h with phorbol 12-myristate 13-acetate (50 ng/ml; Sigma-Aldrich) and ionomycin (500 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: PMA and TTX: MAPK pathways were activated intracellularly via PKC with 1µM-1nM phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, #P1585). PMA treatments were applied as a 2° treatment in place of bicuculine ...
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bioRxiv - Microbiology 2021Quote: ... 300 µL of aliquots (corresponding to 105 to 106 CFU of bacteria) were collected 12 hours post-inoculation and subjected to the treatment with propidium iodide (PI) (Sigma-Aldrich) (concentration- 1µg/ mL ...
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bioRxiv - Molecular Biology 2021Quote: ... of protein were separated by performing SDS-PAGE (10% or 12%) and then transferred onto polyvinylidene difluoride (PVDF) membranes (IPVH00005, Millipore, USA). Membranes were blocked for 1 hours with 5% skim milk at room temperature and then incubated overnight at 4 °C with the primary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... The crude protein was separated by 12% SDS-PAGE and then transferred to a nitrocellulose filter membrane (Millipore, Billerica, MA, USA) using a Trans-Blot Semi-Dry transfer cell (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... RPETert cells were cultured in DMEM/F-12 high-glucose supplemented with 10 % FBS and 100 U Penicillin-Streptomycin (Sigma Aldrich). All cell types were cultured at 37 °C under a 6 % CO2 atmosphere ...
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bioRxiv - Cancer Biology 2021Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were separated by blue native electrophoresis in 3-12% gradient gels (82, 83) using 10mg/ml Ferritin (404 and 880 kDa, Sigma F4503) and BSA (66 and 132kDa ...
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bioRxiv - Biochemistry 2022Quote: ... U937 cells were differentiated to macrophages by 24 h incubation with 20 nM phorbol 12-myristate 13-acetate (PMA, Sigma, P8139) followed by 24 h incubation in RPMI medium ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then left overnight at 37°C before restimulation with 50 ng/mL phorbol 12-myristate 13-acetate (Sigma-Aldrich) and 500 ng/mL ionomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Equal amounts of proteins were electrophoresed on 12% sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and then transferred to polyvinylidene difluoride membranes (PVDF, Millipore, USA). PVDF membrane was blocked with 5% skim milk buffer and then incubated with one of the following primary antibodies ...
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bioRxiv - Bioengineering 2022Quote: ... tumoroids were manually transferred into 12-well culture plates coated with a layer of 2% (w/v) agarose (A9539, Sigma-Aldrich) gel ...
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bioRxiv - Cancer Biology 2022Quote: ... 5–10 μg of total protein per sample were subjected to 10-12% SDS-PAGE and transferred to Immobilon-E membranes (Millipore, #IEVH85R). The blots were developed using Pierce ECL Western Blotting Substrate (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and melan-le cells were cultured in RPMI+10% FBS + 200 nM Phorbol 12-myristate 13-acetate (TPA) (Sigma-Aldrich, P8139).
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bioRxiv - Cancer Biology 2022Quote: ... tumor tissue was minced and digested at 37°C for 12 h in a dissociation buffer containing 0.012% (wt./vol.) collagenase XI (Sigma-Aldrich, C7657) and 0.012% (wt./vol. ...
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bioRxiv - Microbiology 2020Quote: ... 24-well plastic plates were seeded with THP-1 cells at a density of 30 ⨯ 104 cells per well in complete medium after being treated with 30 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich). These 24-well plastic plates where then incubated for 48 hs to induce maturation mediated by PMA toward adherent macrophage-like cells ...
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bioRxiv - Immunology 2021Quote: ... were seeded for 72 h on polycarbonate membrane Transwell permeable inserts (Costar, 3 μm pore size, 12 mm diameter, Sigma Ireland) and incubated for three days ...
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bioRxiv - Microbiology 2021Quote: ... Differentiation of THP-1s into macrophages was achieved by treatment with 25 ng/ml phorbol 12-myristate13-acetate (PMA, Sigma-Aldrich) for 24 h followed by resting in fresh media for another 24 h.
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bioRxiv - Neuroscience 2021Quote: ... Transverse sections of the spinal cord or cortex were embedded in 12% gelatin in 1× PBS (G2500-Sigma Aldrich, St.Louis, MO) and cut via Vibratome to yield 100 μm sections ...
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bioRxiv - Microbiology 2020Quote: ... cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum were differentiated with phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich) 100nM for 48h at a density of 2.105 cells/mL ...
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bioRxiv - Immunology 2020Quote: ... Then the cell extracts mixed with 5 × SDS sample buffer were separated by 12 % SDS-polyacrylamide gel electrophoresis and transferred onto a nitrocellulose membrane (Millipore, USA). Subsequently ...
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bioRxiv - Bioengineering 2021Quote: Proteins were separated on 9% or 12% SDS-PAGE gels in Tris-Glycine buffer and then were transferred into PVDF membrane (Sigma 05317). The blots were then blocked in 3% BSA (w/v ...
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bioRxiv - Immunology 2020Quote: ... THP-1 cells were differentiated into resting macrophages by resuspending the cells in growth medium containing 5 or 50 ng/ml phorbol-12-myristate-13-acetate (PMA; Sigma-Aldrich) and cultured for indicated time periods ...
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bioRxiv - Molecular Biology 2022Quote: ... Tissue lysates were separated by SDS-PAGE gels (4%-12%) and transferred to polyvinylidene difluoride fluorescence membranes (Millipore, Billerica, MA, USA). Non-fat dry milk (NFDM-10% ...
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bioRxiv - Immunology 2022Quote: ... PBMCs cultures not supplemented with IL-2 overnight were stimulated with 25 ng/ml phorbol 12-myristate-13-acetate (PMA) (Sigma-Aldrich) and 1 μg/ml ionomycin (Sigma-Aldrich) ...
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bioRxiv - Genetics 2022Quote: ... coated slides containing 12 wells (Carlson Scientific cat# 101206) were treated by pipetting 30 μl 0.01% poly-L-lysine (Sigma Cat# p4707) into each well and incubating at RT for 15 min ...
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bioRxiv - Genomics 2022Quote: ... The emulsion was solidified at room temperature for 12 hours and beads were removed using 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma Aldrich), washed thrice with Tris–EDTA-Tween buffer (TET ...
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bioRxiv - Molecular Biology 2020Quote: ... The purity of the protein after each step was verified by 12 % SDS-PAGE stained with Coomassie Brilliant Blue R 250 (Sigma-Aldrich). First ...
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bioRxiv - Neuroscience 2021Quote: ... slices were transferred to a 12-well plate with 1 mL freshly oxygenated ACSF and incubated with either 1mM anisomycin (Sigma #A9789) or vehicle (0.5% DMSO ...
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bioRxiv - Developmental Biology 2020Quote: ... Equal volumes from each sample were run on 4-12% Bis-Tris gel (Novex, #NP0322BOX) and transferred onto PVDF membrane (Millipore, #IPFL00010). The membranes were then blocked with Casein-containing buffer (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HNECs were then seeded on 12 mm transwell inserts with 0.4 μm pores (Costar 3460) coated with human placental collagen (Sigma C7521-10MG) at a density of 203,000-333,000 cells per well and maintained in PneumaCult -Ex Plus Medium ...
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Ion transport modulators differentially modulate inflammatory responses in THP-1 derived macrophagesbioRxiv - Immunology 2021Quote: ... the THP-1 cells were differentiated into macrophages by treatment with 200 nM phorbol-12-myristate-13-acetate (PMA) (catalog no. P8139; Sigma-Aldrich) for 3 days.
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bioRxiv - Developmental Biology 2020Quote: ... After 4 days, cells were switched to DMEM/F-12, HEPES (31330038, Thermofischer Scientific) with 2% Horse serum (H1270, Sigma-Aldrich). Before staining ...
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bioRxiv - Immunology 2021Quote: ... Cells (2.5×105 cells/ml) were differentiated with 200 nM phorbol-12-myristate-13-acetate (PMA, Sigma-Aldrich, product no.P-8139) for 48 h ...
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bioRxiv - Immunology 2021Quote: ... THP-1 cells differentiation into mature macrophages-like state was induced through incubation with 10 ng/mL of phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich) for 48 hours in a humidified atmosphere ...
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bioRxiv - Molecular Biology 2021Quote: ... Adherent bone marrow stromal cells were then re-plated in triplicate in 12 well plates at 2×105 cells per well with ascorbic acid and 10 mM β‐glycerophosphate (Sigma) to perform qPCR assays ...
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bioRxiv - Developmental Biology 2021Quote: ... with IgG pull-down as controls (for ab194471, the control was a normal mouse IgG polyclonal antibody, 12-371 from Sigma-Aldrich; for ab31218 and PA5-27431 ...
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bioRxiv - Neuroscience 2021Quote: ... Transverse sections of the spinal cord or cortex were embedded in 12% gelatin in 1X-PBS (G2500-Sigma Aldrich, St.Louis, MO) and cut via Vibratome to yield 100 µm sections ...
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bioRxiv - Microbiology 2020Quote: ... then 5×106 PFUs were resolved on an 8-12% SDS-PAGE gel and transferred to an Immobilon-FL polyvinylidene difluoride membrane (Millipore, IPEL00010). Membranes were probed for 30 min at room temperature or 4°C overnight with antibodies as follows ...
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bioRxiv - Cell Biology 2020Quote: ... Equivalent amounts of total protein were loaded into a 12% SDS-PAGE for electrophoresis and then transferred into a PVDF membrane (Millipore, USA). After blocking nonspecific binding with 5% skimmed milk in TBST ...
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bioRxiv - Microbiology 2021Quote: ... 2 ml of conditioned medium were dialysed with dialysis tubing cellulose membranes (Sigma-Aldrich, cut-off 12 kDa, REF: D9777-100FT) against 50 ml DTM supplemented with 15 % FBS at 4 °C in two rounds (16 hours ...
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bioRxiv - Microbiology 2020Quote: ... which was added to the growth media from an Fe(III)-citrate solution prepared by mixing Fe(III)Cl3 (6 mM) and citric acid (12 mM) powders (Sigma-Aldrich) in Milli-Q water ...
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bioRxiv - Microbiology 2020Quote: ... The heated samples were subjected to 12% (v/v) SDS-PAGE and then electrophoretically transferred onto a nitrocellulose membrane (320mA for 2h) (Sigma-Aldrich) in transfer buffer [25mM Tris–HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... The purity of the protein after each step was assessed by 12% SDS-PAGE stained with Coomassie Brilliant Blue R 250 (Sigma-Aldrich). First ...
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bioRxiv - Immunology 2020Quote: ... 2mM glutamine and 100 U/mL penicillin and 50 µg/mL streptomycin and differentiated into macrophages after treatment with 50 ng/ml phorbol-12-myristate 13-acetate (PMA, Sigma Aldrich) for 48 hours (8,19).
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bioRxiv - Immunology 2022Quote: ... Macrophages were generated by differentiating THP-1 monocytes with 100 ng/ml phorbol 12-myristate 13-acetate (PMA) (Millipore Sigma, P8139) for 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... for approximately 12-18 hours and then subjected to shear stress (130rpm) in new medium containing 4% dextran (Sigma-Aldrich #31392) for indicated time intervals and cultured alongside static controls ...
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bioRxiv - Bioengineering 2022Quote: ... ATCC® CRL-4003) were maintained as per the manufacturer’s instructions in custom phenol red-free DMEM/F-12 (based on Sigma #D 2906) supplemented with 1% ITS+ Premix (Corning 354352) ...
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bioRxiv - Biophysics 2022Quote: ... NaCl and indium titanium oxide (ITO) coated glass slides (surface resistivity 8–12 V sq-1) were purchased from Sigma-Aldrich Co ...