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Citations for Millipore Sigma :
3001 - 3050 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... To prevent attachment of cells all dishes/plates were coated with polyhydroxyethylmethacrylate (PolyHEMA) (12 mg/ml in 95% ethanol, Sigma-Aldrich, Germany) overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Baseline fluorescence was recorded for 30s before application of either vehicle or 1μM phorbol 12-myristate 13-acetate (PMA) (Sigma Aldrich, Cat# P8139) and imaged every 10s for 5 minutes at 50% laser power ...
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bioRxiv - Bioengineering 2021Quote: ... Characterization of protein and mRNA expression was conducted using 100 μL hydrogels prepared in 12 mm Millicell® 04 μm PTFE cell culture inserts (PICM01250; EMD Millipore) receiving 800 μL HeptoSTIM™ media every 72 h ...
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bioRxiv - Systems Biology 2020Quote: ... For macrophage differentiation U937 cells were suspended in medium with 20 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich, #P1585) and plated in 96-well microplates with µClear®flat bottom (Greiner ...
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bioRxiv - Microbiology 2021Quote: Cell migration and invasion assays were performed using 24-well transwell chambers with filter membranes 12 µm pore size (Millipore Corporation, PIXP01250). Cells were detached with trypsin-EDTA ...
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bioRxiv - Molecular Biology 2020Quote: Production of shRNA-expressing lentiviral particles was performed as described previously (12) using plasmids expressing shRNAs targeting MRE11 (Sigma mission shRNA TRCN000338391), NBS1 (Sigma mission shRNA TRCN0000288622 ...
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bioRxiv - Cancer Biology 2020Quote: HL60 cells were plated at a density of 4×105 cells/ml and differentiated with 12-O-tetradecanoylphorbol-13-acetate (TPA) (Sigma, Darmstadt, Germany) at a final concentration of 32 nM for 6 days ...
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bioRxiv - Cell Biology 2020Quote: ... organelle and nuclear fractions were harvested every 12 h for 2 days using the Nuclei EZ Prep nuclei isolation kit (Sigma NUC-101) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The remaining cell-free medium (12 x 40 ml) was then filtered through a 0.45 µm HV/PVDF (Millipore, ref. no. SE1M003M00) and concentrated ∼100 times by resuspending the viral pellet obtained by ultracentrifugation at 70’000 g for two hours at 4°C in ∼5 ml ice-cold PBS ...
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bioRxiv - Cell Biology 2020Quote: ... Lipids were resuspended in 2-propanol (15 µL) and transferred to amber glass vials (Sigma; QSertVial™, 12 × 32 mm, 0.3 mL). Sodium borohydride (15 µL of 10 mM in 2-propanol ...
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bioRxiv - Cancer Biology 2021Quote: ... 15 μg of tumor protein was resolved on 12% SDS-PAGE gels and transferred to Immobilon-FL PVDF membrane (Millipore, catalog# IPFL00010). Membranes were blocked with 5% (w/v ...
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bioRxiv - Immunology 2022Quote: ... A 15 ul of lysate was resolved on 12% SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Burlington, MA). The membrane was blocked in 5% powder milk overnight in 4°C ...
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bioRxiv - Pathology 2022Quote: ... Twenty micrograms of protein per lane were loaded onto an 8-12% polyacrylamide Gel and then transferred to PVDF membranes using an electrophoresis chamber (Millipore, Shanghai, China) for 1.5 h ...
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bioRxiv - Cancer Biology 2022Quote: HEK293T cells at 80-90% confluency in a 12-well plate were transfected using 6 μL GeneJuice Transfection Reagent (Sigma-Aldrich, 70967) and 100 μL RPMI 1640 medium (ThermoFisher ...
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bioRxiv - Molecular Biology 2020Quote: Confluent monolayers of HAEC grown on 6-well plate were exposed to unidirectional PSS (6, 12 and 24 hours) using a modified flow device.10 Neutralized MCDB-131 medium (Sigma Aldrich, MO) containing 7.5% sodium bicarbonate solution ...
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bioRxiv - Biochemistry 2021Quote: ... 12 μg peptides were adjusted to pH 7-8 by adding one volume of 50 mM ammonium bicarbonate (ABC, Sigma-Aldrich, A6141). Offline fractionation by high-pH reversed-phase chromatography was carried out on an Ultimate 3000 HPLC (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and macrophage-like differentiation was induced upon a twenty-four hours treatment with 100ng/ml of phorbol 12-myristate 13-acetate (PMA) (Sigma cat. P1585). Calu-3 cells media was instead supplemented with a 1x final concentration of non-essential amino-acids (Sigma cat ...
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bioRxiv - Immunology 2022Quote: ... coli XL 1 blue and K-12 MG1655 were cultured overnight in Luria Bertani (LB) broth media (Sigma Aldrich, cat. no: L3022). Overnight cultures were diluted to OD 600 ∼0.5 ...
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bioRxiv - Bioengineering 2022Quote: ... 0.25 mg mL−1) was injected into mini dialysis tubes (molecular weight cutoff of 12 – 14 kDa; Sigma-Aldrich Pur-A-Lyzer). Each tube (n = 3 ...
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bioRxiv - Pathology 2021Quote: ... Infected tissues were disrupted and digested overnight (12 hours) using Fast Prep® Lysing Matrix tubes containing 0.2 mg (6 U) Proteinase K (Sigma-Aldrich®) diluted 1/40 in Lifton’s Buffer (100 mM EDTA ...
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bioRxiv - Immunology 2020Quote: ... Cultured cell lysates (30 μg) were electrophoresed in an 8–12% SDS-PAGE gel and transferred to a PVDF membrane (Millipore, MA, USA). PVDF membranes were blocked with 5% skim milk in phosphate buffered saline with 0.1% Tween 20 (PBST ...
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bioRxiv - Systems Biology 2020Quote: ... we split and seeded iPS-GM942-SeV3 into Geltrex-coated 12-well plates at a density of 3 × 105 cells per well in iPS-Brew + 2μM Thiazovivin (Sigma, cat. SML1045-5MG). After 24 hours ...
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bioRxiv - Physiology 2020Quote: DN was induced experimentally in 12-h fasting rats except for the normal group through a single intraperitoneal injection of 0.5 mL STZ (Sigma-Aldrich, Deisenhofen, Germany) at a dosage of 60 mg/kg of body weight ...
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bioRxiv - Molecular Biology 2021Quote: ... the protocol described by Choi et al.65 was applied using home-made wash buffer (50% formamide (Merck Millipore, 75-12-7), 5XSSC (Molecular Biology-P ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Approximately 40 μg of protein was separated on 12% SDS-polyacrylamide gel (SDS-PAGE) and the gel was transferred to polyvinylidene difluoride (PVDF) membrane (Millipore, MIT, USA). The membrane was blocked with 5% nonfat dry milk in TBST (Trisbuffered saline/0.1% Tween-20 ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.01% bromophenol blue) and loaded onto 10-12% SDS-polyacrylamide gels and proteins were transferred to nitrocellulose membranes (Merck-Millipore, Berlin, Germany). Afterwards ...
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bioRxiv - Cell Biology 2020Quote: ... the membrane was washed once with TBST and incubated with primary antibodies at 4°C for 12 hrs (Phospho-ERK1/2 1:500 (Sigma Aldrich, #E7028); ERK1/2 1:1000 (Cell Signaling ...
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bioRxiv - Immunology 2020Quote: ... cell suspensions in RPMI medium supplemented with 10% FBS were re-stimulated for 4 hr with phorbol 12-myristate 13-acetate (PMA, Sigma, 50ng/mL) and ionomycin (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... As a positive control we used a bona fide Src substrate peptide corresponding to amino acids 6-20 of p34cdc2 (Millipore-Sigma, 12-140). The reactions were carried out at room temperature in a total volume of 25 μL for 40 min in white 96-well ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were thawed at room temperature in DMEM/F-12 supplemented with 10% FBS and a protease inhibitor cocktail (Sigma Aldrich 11836170001). Atomic force microscopy (AFM ...
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bioRxiv - Cell Biology 2022Quote: ... cells were released into DMEM/F-12 medium for 5 hours and then treated with 100 ng/ml nocodazole (Sigma, Cat# M1404) in DMEM/F-12 medium for 4 hours to arrest cells in metaphase ...
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bioRxiv - Cell Biology 2022Quote: ... 1×105 HEK 293T cells were re-suspended in 0.5 mL of complete media and seeded into each well of a 12-well dish (Millipore Sigma Cat. CLS3513). On the next day ...
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bioRxiv - Cell Biology 2022Quote: ... shaking at 60 °C) and alkylated (addition of 12 µL x 500 mM iodoacetamide, Sigma, in AB; 30 min. shaking at RT). Next ...
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bioRxiv - Molecular Biology 2022Quote: ... German glass 18 mm #1 coverslips (Electron Microscopy Services, 72292-09) placed in a 12-well dish were washed in 3 M sodium hydroxide (Millipore Sigma, 221465) for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: wtKSHV inoculum was obtained from BCBL-1 cells (5×105 cells/ml) induced with 20 ng of TPA (12-O-tetradecanoylphorbol-13-acetate; Sigma, St.Louis, USA)/ml ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were seeded at a density of 1x105 cells/ml in 96 well plates and differentiated for 48 hours with 25 nm PMA (phorbol-12-myristate-13-acetate, Sigma-Aldrich, #P1585). Cells were then left to rest for 24 hours in fresh media containing no PMA prior to bleomycin exposures.
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bioRxiv - Neuroscience 2022Quote: ... was carbon filtered and passed through PFA vials (Savillex 200-30-12) containing odor (2% methyl valerate in mineral oil, Sigma Aldrich product #1489977; 2% 2-heptanone in mineral oil, Sigma Aldrich product # 537683 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated on 6-well cell culture-treated plates and differentiated using 100 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, P1585) for 72 h in serum-free RPMI 1640 medium (SFM ...
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bioRxiv - Molecular Biology 2022Quote: ... Stimulation of Jurkat cells for 2-7 hour experiments was achieved with 50ng/ml Phorbol 12-myristate 13-acetate (PMA, Sigma-Alrich, P8139) and 500ng/ml ionomycin calcium salt from Streptomyces conglobatus (ionomycin ...
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Kynurenic acid promotes A1 to A2 astrocyte conversion against HIV-associated neurocognitive disorderbioRxiv - Microbiology 2022Quote: ... Cultured astrocytes were treated with 150 pM HIV-1 gp120 CM (ProSpec-Tany TechnoGene Ltd., Israel) for 12 h before application of 5 or 25 μM KYNA (Sigma-Aldrich, USA) for a further 12 h ...
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bioRxiv - Neuroscience 2022Quote: ... animals were first pre-incubated for 10 minutes in low Mg2+ HL3 (10 mM Mg2+, 0 mM Ca2+) containing 2 µM of phorbol ester Phorbol-12-myristat-13-acetat (PMA (Sigma Aldrich, Germany), stored at −20°C as 10 mM stock in DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... animals were pre-incubated for 10 minutes in HL3 (0 mM Ca2+) containing 2 µM of phorbol ester Phorbol-12-myristat-13-acetat (PMA (Sigma Aldrich, Germany), stored at −20°C as 10 mM stock in dimethyl sulfoxide (DMSO)) ...
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bioRxiv - Microbiology 2024Quote: Approximately equal amounts of GST fusion proteins and alanine-mutated GST fusion proteins were separated by 12% SDS-PAGE and transferred to PVDF transfer membranes (Millipore, MA, USA). The membranes were blocked with 5% skimmed milk powder for 2 hours at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... the solution was diluted with warm PBS (40°C) and dialyzed (with 12-kDa molecular weight cut-off membrane; Sigma Aldrich, USA) against distilled water for 5-7 days at RT to remove excess methacrylic acid and other impurities ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then run on NuPAGE 4-12% Bis-Tris Protein Gels (Thermo #NP0335BOX) and transferred to a PVDF membrane (EMD Millipore #IPVH00005). Blots were probed with primary antibodies overnight at 4°C in 5% blocking solution ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.8 µg pCMV-VSV-G (lentiviral envelope plasmid) and 2 µg purified shRNA DNA with 12 µg (3:1) of X-tremeGENE transfection reagent (Sigma Aldrich, #6366244001). Following 72 hours of transfection ...
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bioRxiv - Microbiology 2024Quote: ... cell differentiation into macrophages was induced by adding 50 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, St. Louis, MO, USA) to the medium for 72 hours.
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bioRxiv - Cell Biology 2024Quote: ... 20 µg proteins were separated by SDS-PAGE (10– 12% gels) and were blotted onto a nitrocellulose membrane (Millipore, Billerica, MA, USA). The membrane was blocked with 5% BSA in TBST at room temperature for 1h ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... were electrophoresed on SDS polyacrylamide gels ( NuPAGE™ 4-12% Bis-Tris Protein Gels; InvitrogenTM) and transferred to PVDF membranes (Millipore; Merck). Membranes were blocked in 2.5% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... 10% or 12% polyacrylamide gels before being transferred to PVDF Immobilon-FL membrane with pore size 0.45 μm (Millipore Billerica, MA, USA) using a Mini Trans-Blot electrophoretic transfer system (BioRad ...