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Citations for Millipore Sigma :
2551 - 2600 of 10000+ citations for Anti Epidermis type lipoxygenase 12 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Per condition 20 million MRC-5 cells were transduced at 75% confluency in 145 cm2 dishes with concentrated lentivirus diluted in 18 ml of culture medium supplemented with 12 µg/mL polybrene (Sigma). The virus titer was determined to achieve a multiplicity of infection of <0.25 ...
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bioRxiv - Cell Biology 2021Quote: ... GMPS were sorted as described above from CD45.1 and cells were cultured in a 12 well plate pre-coated with human fibronectin (EMD Millipore) in RPMI1640 media + 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Cell Biology 2020Quote: ... with 5 ml fully supplemented media and 100 ng/ml PMA (Phorbol 12-myristate 13-acetate ≥99%, Sigma-Aldrich, Austria) to induce differentiation ...
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bioRxiv - Cell Biology 2021Quote: ... Neurons were plated at a density of ∼3 × 105 cells on 12-mm glass coverslips coated with 0.01% poly-L-lysine (Sigma, P4832). Half of the medium was changed every 3-4 days and experiments were performed at 12–13 days in vitro.
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bioRxiv - Cell Biology 2020Quote: ... Western blotting was performed on 12% SDS-PAGE gels using 1-hour semi-dry transfer with 3% bovine serum albumin (BSA, Sigma) in TBS + 0.5% Tween-20 as a blocking agent.
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bioRxiv - Cancer Biology 2020Quote: ... BT-12 coverslips and Transwell membranes containing both bEND3 and HIFko cells were cut and mounted on Mowiol 4-88 (Sigma) and imaged on a Zeiss LMS880 confocal microscope.
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bioRxiv - Neuroscience 2022Quote: The monolayer tightness in 12-well inserts was also assessed by measuring LY (Lucifer Yellow CH dilithium salt, Sigma-Aldrich) permeability as previously described (Molino et al. ...
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bioRxiv - Neuroscience 2022Quote: ... the mice were exposed to 12 μl of 2,4,5-Trimethylthiazole (TMT; synthetic extract of fox urine; ≥98% purity; Sigma-Aldrich, UK) pipetted on a small circular piece of filter paper in a petri dish placed in the centre of the cage for 20 min ...
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bioRxiv - Cancer Biology 2022Quote: Leukemia cells were seeded at a density of 2.5 x 105 cells/well of a non TC-treated 12-well plate in complete medium containing polybrene (10µg/mL, EMD Millipore), and then transduced with lentivirus by centrifugation at 2,500rpm for 90 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... THP-1 monocytes were differentiated into macrophages in RPMI + 10 mM HEPES + 10% FBS + 10 ng/mL phorbol 12-myristate 13-acetate (PMA; Sigma) and seeded at 1×105 cells per well of a 96-well tissue culture dish and further treated with 66.8 μM concentration of bifidococcin664 separately ...
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bioRxiv - Cell Biology 2022Quote: Fat bodies were dissected from larvae at 5 or 12 days AEL fixed in 4% PFA and counterstained with Nile Red (Sigma), Phalloidin-488 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham (DMEM/F12) and bovine serum albumin (BSA) from Sigma (Dorset, UK); recombinant human HMGB1 (C-terminal His tag ...
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bioRxiv - Biochemistry 2022Quote: ... Membrane translocation in live cells was induced by the addition of 500 nM phorbol 12-myristate 13-acetate (PMA; Sigma) and monitored by imaging in 20–30 s intervals ...
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bioRxiv - Bioengineering 2022Quote: THP-1 cells plated at a seeding density of 35000 cells/well in a 96-well plate were activated with 20 ng/mL phorbol 12-myristate 13-acetate (Sigma) and incubated ...
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bioRxiv - Biophysics 2022Quote: ... 1-oleoyl-2-(12-biotinyl(aminododecanoyl))-sn-glycero-3-phosphoethanolamine (biotin-PE) and Tween-20 were purchased from Sigma Aldrich. Tween-20 was suspended in 50 mM Tris buffer (pH 8 ...
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bioRxiv - Cancer Biology 2022Quote: Freshly isolated tissue-specimens were washed in Dulbecco’s Phosphate Buffer Solution (DPBS) and fixed overnight (12-18 hours) in a 10% formalin solution (Sigma, HT501320). Formalin-fixed ...
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bioRxiv - Molecular Biology 2022Quote: ... Ten micrograms of sample were separated on 10-12% SDS-PAGE gels prior to transfer onto Immobilon-P PVDF membranes (Millipore). Membranes were blocked in 5% milk and incubated overnight with primary antibodies diluted in 5% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were cleared by centrifugation at 17,000 g 12 min and input aliquot was incubated with 6 mM MgCl2 and Benzonase (1:75, EMD Millipore). The remaining lysate was diluted with 300 μl of dilution buffer + supplements and incubated with 25 μl of GFP- or Myc-Trap magnetic agarose beads for 2 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and megakaryocyte-like differentiation was induced with 10 nM or 50 nM 12-O-Tetradodecanoyl-phorbol-13 acetate (TPA, Sigma) for 24 hrs ...
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we fed animals after 0 h and 12 h with dextran beads (Sephadex ‘Small’ by Sigma Aldrich: 50 mm diameter) at a concentration of 0.5 g/100 mL [55] ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were analyzed by separation on SDS-PAGE gels (8-12%) and transferred to polyvinylidene difluoride (PVDF) membrane (EMD Millipore). Immunoblots were imaged using an anti-Ssa1/2 polyclonal antibody at a 1:10,000 dilution ...
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bioRxiv - Cancer Biology 2022Quote: ... and 50μg of protein was resolved on 10–12% SDS-PAGE followed by transferring to nitrocellulose or PVDF membranes (EMD-Millipore). For immunoprecipitation ...
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bioRxiv - Neuroscience 2020Quote: ... the hippocampal cells were seeded on a coverslip (12 mm diameter) coated with poly-L-lysine in DMEM (Sigma-Aldrich) and incubated for 4 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... boiled at 95°C for 5 min and subjected to 12% SDS-polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride (PVDF) (Millipore) at 20 V/cm for 1h ...
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bioRxiv - Neuroscience 2020Quote: ... 50 −100 μg of protein were loaded on a 7%-12% polyacrylamide gel and then transferred on a methanol activated PVDF membrane (Millipore). Blot membranes were blocked for one hr with 5% milk or 5% BSA in TBS with 0.1% Tween 20 (TBST) ...
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bioRxiv - Immunology 2020Quote: ... IELs were plated in 48-well plates and incubated at 37°C with 100ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma) and 200ng/mL ionomycin (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Maturation of THP-1 cells into macrophages was induced by adding 200 nM PMA (phorbol 12-myristate 13-acetate; Sigma) in cell culture medium for 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots of cell lysate were resolved on 10% to 12% SDS-polyacrylamide gels and subsequently transferred to a polyvinylidene difluoride membrane (Millipore). The membrane was incubated with the following primary antibodies ...
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bioRxiv - Developmental Biology 2021Quote: ... dispase was inactivated by adding 80µL of cold DMEM/12 supplemented with 5% bovine serum albumin (BSA; Sigma-Aldrich, A8577) into each well ...
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bioRxiv - Biochemistry 2021Quote: ... and untreated Ycf1 protein (12 μg) sample were incubated for 30 min on ice with trypsin from bovine pancrease (Sigma) at varying concentrations (0 ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellet was resuspended in buffer A supplemented with 200 μg/mL of heparin and lysed by 10–12 cycles of 30 sec vortexing followed by 30 sec incubation on ice in the presence of glass beads (Sigma). Cell lysates were collected by centrifugation at 21,000 × g for 15 min in a tabletop centrifuge at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells were seeded on 12-well plates with glass coverslips (Marienfeld Superior) coated with poly-L-lysine (Sigma-Aldrich) one day prior to transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... MDA-MB-231 and SUM1315MO2 cells were cultured in 10% FBS/DMEM with added Ham’s F-12 nutrient mixture (Sigma-Aldrich) and supplemented with 1x MEM Non-Essential Amino Acids Solution (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... 1mL of HEK 293F cells from each batch was mixed together in a 12-well Corning Costar Not Treated Plate (Millipore) and incubated at 37°C with shaking on Orbi-Shaker (Benchmark ...
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bioRxiv - Neuroscience 2021Quote: ... plated at a density of 60,000 cells on 12 mm cover glasses pre-coated with 1 mg/ml poly-l-lysine (Sigma, P1274), and treated at 2 div for 24 with Ara-C (2 μM ...
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bioRxiv - Neuroscience 2021Quote: ... cells were approximately 80% confluent and passaged at 1:4 from 12-well plates to 15µg/mL Poly-L-Ornithine (Sigma-Aldrich) + 10µg/mL Laminin (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The cell pellet was collected for SDS-PAGE analysis (4% stacking gel, 12% running gel) and the gel was stained by colloidal Coomassie brilliant blue (Sigma).
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bioRxiv - Microbiology 2020Quote: ... Samples containing equal amounts of protein were separated by SDS-PAGE (4% stacking and 12% resolving gel) and transferred onto a PVDF membrane (Millipore). The membrane was blocked with 5% skim milk (for detection of E ...
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bioRxiv - Neuroscience 2021Quote: ... Days 12 through 15 received N3 without additional supplements and beginning on day 16 0.05uM Retinoic acid (Millipore Sigma #R2625) was added to the basal N3 media ...
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bioRxiv - Neuroscience 2021Quote: ... was induced by a single intra-articular injection of 12 μl of Complete Freund Adjuvant (CFA, heat-killed Mycobacterium butyricum 2 mg/ml, Sigma) under isoflurane (2.5% ...
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bioRxiv - Immunology 2021Quote: ... equal amounts of cell lysates and immunoprecipitants were resolved by 10-12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and were then transferred to a polyvinylidene difluoride (PVDF) membrane (Millipore). After incubation with primary and secondary antibodies ...
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bioRxiv - Biophysics 2021Quote: ... The eluted protein fractions were concentrated to ∼12 mL using an Amicon Ultra-50 centrifugal filtration device (100-kDa MW cutoff, Millipore) and loaded onto a HiPrep Desalting column 26/10 (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... THP1 monocytes were differentiated into M0 macrophages by exposure to phorbol 12-myristate 13-acetate (100 ng/ml; Sigma Aldrich) for 48h ...
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bioRxiv - Bioengineering 2020Quote: ... were seeded into 96-well plates and incubated for 24 h in the presence of 15 ng mL−1 of phorbol 12-myristate 13-acetate (PMA, Sigma). The medium was removed and cells were washed with PBS ...
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bioRxiv - Pathology 2022Quote: ... splenocytes (1 million cells from each animal) was stimulated with PMA (phorbol 12-myristate13-acetate; 50 ng/ml; Sigma-Aldrich), ionomycin (1.0 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were seeded at a concentration of 110,000/well in 12-well plates on ø 18mm glass coverslips coated with 0.1 mg/ml poly-ornithine (Sigma-Aldrich) and 1 μg/ml laminin (Corning) ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was run on 12% acrylamide SDS-PAGE gel at a final concentration of 1 µM and transferred to polyvinylidene difluoride (PVDF) membrane (Millipore) using a semi-dry transfer apparatus ...
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bioRxiv - Cancer Biology 2022Quote: ... tissues from patients prior to chemotherapy were provided for processing (laboratory of HNL) and collected in chilled DMEM/F-12 media (Dulbecco’s Modified Eagle Medium; Millipore-Sigma). Tumor cells were either isolated on the day of collection or the next day (stored in media at 4°C until isolation) ...
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bioRxiv - Molecular Biology 2022Quote: ... For Huh7-dCas9 knockdown in 12-well dishes 30 µL of each gRNA was combined with 7 µL TransIT-CRISPR transfection reagent (Sigma) in 363 µL OPTI-MEM (Life Technologies ...