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Citations for Millipore Sigma :
2601 - 2650 of 2658 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... K986P and V987P (S-2P) (70–72) was generated by PCR mutagenesis using KOD Xtreme Hot Start DNA Polymerase (Sigma, 71975-M). pcDNA3-Membrane (M ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were obtained from ATCC and were tested negative for mycoplasma using LookOut® PCR-based kit (MP0035-1KT Sigma-Aldrich). HEK293-Klotho cells stably expressing Klotho were a gift from Dr ...
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bioRxiv - Plant Biology 2019Quote: ... were sourced from Nottingham Arabidopsis Stock Centre and homozygous mutants where confirmed through genotyping PCR on genomic DNA using REDTaq® ReadyMix™ (Sigma) following their protocol ...
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bioRxiv - Genomics 2019Quote: ... Real time PCR was carried out in a Stratagene MX3005P thermocycler using SYBR Green Jump Start Taq Ready Mix (Sigma Aldrich, USA) for quantifying amplification results ...
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bioRxiv - Microbiology 2019Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). For E ...
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bioRxiv - Genomics 2021Quote: ... we performed up to 8 nested PCR reactions in parallel with an input of 100-200ng per reaction using the KAPA2G Robust ready mix (Sigma-Aldrich, KK5702) in a volume of 50μL ...
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bioRxiv - Cancer Biology 2021Quote: ... Aforementioned single clone cultures were STR validated and screened for any mycoplasma contamination using PCR based Myco detection kit (Sigma, MP0035-1KT) before use ...
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bioRxiv - Cancer Biology 2021Quote: ... The real-time PCR analysis was also performed with the Complementary DNAs using JumpStart Taq DNA polymerase (Sigma-Aldrich, St. Louis, MO) and SYBR green nucleic acid stain (Molecular Probes ...
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bioRxiv - Cell Biology 2021Quote: DNA extraction from mouse tail clips obtained at 7-10 days of age was performed using REDExtract-N-Amp™ Tissue PCR Kit (Cat#R4775, Sigma Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomatobioRxiv - Molecular Biology 2019Quote: ... DNA amplicons and related donor template levels were evaluated by semiquantitative PCR and qPCR (using KAPA SYBR FAST qPCR Kits, Sigma-Aldrich, USA), respectively ...
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bioRxiv - Microbiology 2020Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). Chlamydia trachomatis strain L2/434 Bu was obtained from the laboratory of Dr ...
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bioRxiv - Microbiology 2022Quote: ... The amplified PCR products were visualized by 1.5% agarose gel electrophoresis and purified using a Millipore filter plate MSNU030 (Millipore SAS, Molsheim, France). The purified PCR products were then subjected to Sanger sequencing using the BigDye Terminator v3.1 sequencing kit and a 3730xl automated sequencer (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... PCR was performed using either KAPA HIFI 2X ready mix (KAPA Biosystem, cat# KK2602) or Expand Long Template PCR System (Sigma, cat# 11681842001). Primers used for each PCR reaction are listed in Table S4.
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bioRxiv - Immunology 2024Quote: ... Cell lines were kept at 37°C in 5% CO2 and routinely checked for mycoplasma contamination via LookOut Mycoplasma PCR detection kit (Sigma, Cat. MP0035).
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bioRxiv - Cancer Biology 2023Quote: ... a polymerase chain reaction (PCR)-based method was used to screen for mycoplasma once a month employing the LookOut® Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich) and JumpStart™ Taq DNA Polymerase (D9307 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Real-Time PCR reactions were performed in 20 µL mixtures containing 10 µL KAPA SYBR FAST qPCR Master Mix (2X) (Sigma-Aldrich, KK4605), 200 nM final concentration of species-specific rpoD forward and reverse primer and up to 3 µL of sample template ...
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bioRxiv - Microbiology 2023Quote: ... All cell lines were tested every two weeks for mycoplasma contamination either by in-house DAPI staining or LookOut® Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Genetics 2023Quote: ... blots were hybridized with Digoxigenin (DIG)-labeled DNA probes (Supplementary Table 2) amplified by the PCR DIG Probe Synthesis Kit (Sigma-Aldrich; 11636090910). The hybridized probe was immunodetected with anti-digoxigenin Fab fragments conjugated to alkaline phosphatase (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... through a 130 μm low-pressure deposition nozzle into 96 well PCR plates containing 10 µL 1x prepared Cell Lysis and Fragmentation Buffer Mix (Millipore Sigma, WGA4). Doublets were excluded from analysis via standard FSC/SSC gating procedures ...
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products from each targeted exon were purified and mixed with 0.5 units of recombinant spCas9-GFP (Sigma-Aldrich, Cat # CAS9GPRO), 10 μM gRNA in 2 μL of 10x Cas9 reaction buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... The locus with expected insertion was amplified by PCR using 5 μL KOD Hot Start 2× Master Mix (Sigma Aldrich 71842-3), 0.2 μL each primer at 10 μM ...
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bioRxiv - Neuroscience 2024Quote: All cell cultures were tested for mycoplasma contamination once per month using LookOut® Mycoplasma PCR Detection Kit (Sigma, cat.no. MP0035-1KT).
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bioRxiv - Microbiology 2024Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). For E ...
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bioRxiv - Molecular Biology 2021Quote: ... The genotyping of the DNA extracted from ears of transgenic mice by Extract-N-Amp™ Tissue PCR Kit (Sigma St. Louis, MO) was performed following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2019Quote: ... PCR reactions were analyzed by gel electrophoresis using a 1% agarose gel in TAE buffer (20 mM acetic acid (Sigma Aldrich, cat#, 695092), 2 mM EDTA (ACROS Organics ...
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bioRxiv - Immunology 2019Quote: ... were designed using the chop-chop website (55) and generated by PCR complementation and amplification of full length ssDNA oligonucleotides (Sigma-Aldrich, Table S3) as described (19) ...
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bioRxiv - Biochemistry 2020Quote: DNA encoding for Pry1 and Na-ASP-2 were PCR amplified and cloned into NcoI and XhoI restriction sites of pET22b vector (Novagen, Merck, Darmstadt, Germany), which contains a pelB signal sequence to direct the secretion of expressed protein into the periplasmic space ...
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bioRxiv - Cell Biology 2020Quote: Mouse genomic DNA was isolated from tail biopsies using a specific kit (Extract-N-Amp™ Tissue PCR Kit; Sigma-Aldrich, Darmstadt, Germany). PCR was performed using the primer pairs to amplify the DHX15 gene (primer forward ...
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bioRxiv - Biochemistry 2022Quote: ... were authenticated by short tandem repeat (STR) profiling and tested for mycoplasma contamination prior to experiments using the LookOut® Mycoplasma PCR Detection Kit (Sigma, MP0035-1KT). HEK293T (ATCC CRL-3216 ...
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bioRxiv - Microbiology 2020Quote: Full-length sequences of selected sdAbs were PCR amplified and cloned into the NcoI/XhoI sites of the pET28b (Novagen, Sacramento, CA, USA) and transformed into BL21(DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... An amount of 50 μg of Co-IP sample was transferred to a PCR tube and incubated with 1 μL of benzonase (Sigma, cat. no. E8263) at 37°C for 30 min to shear and digest DNA ...
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bioRxiv - Microbiology 2021Quote: Full-length sequences of selected nanobodies were PCR amplified and cloned into the NcoI/XhoI sites of the pET28b (Novagen, Sacramento, CA, USA) and transformed into BL21(DE3 ...
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bioRxiv - Microbiology 2024Quote: ... [22]) were used to amplify the 16S V4 variable region in PCR reactions using Kapa HiFi Hotstart ready mix (Sigma-Aldrich, Oakville, Canada) (Tables S2 ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR products were purified and used for one-step isothermal assembly with EcoRV-digested pFLAG-CMV-5.1 (Sigma, St. Louis, MO, USA)32.
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bioRxiv - Microbiology 2023Quote: ... All bacterial and eukaryotic cell stocks were confirmed to be Mycoplasma negative using the LookOut Mycoplasma PCR Detection Kit (Sigma, St. Louis, MO). Molecular biology reagents were purchased from ThermoFisher unless otherwise noted.
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bioRxiv - Cell Biology 2023Quote: ... were generated by PCR and cloned into the pFlag-CMV4 plasmid (for N-terminal Flag fusion in mammalian cells, cat#E7158, Merck Millipore, Burlington, MA, USA). CDS of ARVA ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 100,000 nuclei were sorted at a ratio of 70% NeuN+ and 30% NeuN-into a single well of a PCR plate (Sigma-Aldrich, EP0030129504-25EA) containing 50 µL FANS buffer (see Table S8) ...
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bioRxiv - Plant Biology 2020Quote: ... An equal concentration of cDNA from the samples comparing was used for qRT-PCR reaction with KAPA SYBR Fast qPCR Kit (Sigma-Aldrich, St. Louis, USA). For expression analysis of the genes responsive to nutrient starvation ...
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bioRxiv - Plant Biology 2020Quote: ... the ORF of CLEL6 lacking the predicted signal peptide was amplified by PCR from a previous construct and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). Correct orientation and translational fusion with the C–terminal His-tag was verified by sequencing ...
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bioRxiv - Plant Biology 2021Quote: ... was amplified by PCR as previously described (Rey et al., 2018b) and labelled using the DIG-nick translation mix (Sigma, St. Louis, MO, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... ORFs of PSK1 and RGF1 lacking the predicted signal peptides were amplified by PCR from cDNA and cloned into the NcoI restriction site of pETDuet1 (Novagen/Merck KGaA, Darmstadt, Germany). C-terminal His tags were added by including 6 His codons in the reverse PCR primers ...
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bioRxiv - Microbiology 2022Quote: ... The fractions containing the PCR product was pooled and concentrated by a minimum cut-off of 10 MWCO spin column (Amico Ultra, Millipore Sigma, Burlington, MA, USA) in 0.5X TE.
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bioRxiv - Microbiology 2021Quote: ... All specimens were quick frozen in triplicate over liquid nitrogen for qRT-PCR analysis or collected into RNAlater (cat# R0901, Sigma-Aldrich, St. Louis, MO). Multiple blocks of tissues were collected in histology cassettes fixed in 10% buffered formalin ...
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bioRxiv - Plant Biology 2020Quote: ... and DNA was extracted at the field using quick Extract-N-Amp™ Tissue PCR Kit (Sigma-Aldrich Co. LLC, MO, Louis. St; USA) with some modifications ...
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bioRxiv - Genetics 2020Quote: PCR genotyping of all mouse strains was performed on genomic DNA obtained from tail biopsies digested with Proteinase K (Sigma, St. Louis, MO, USA) using primers listed in Table S1.
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bioRxiv - Genomics 2020Quote: ... DNA was eluted in 17 μl of water and 16 μl was then transferred to a new PCR tube and denatured by addition of 4 μl of formamide (Sigma-Aldrich, St. Louis, MO) and incubation at 85°C for 10 min ...
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bioRxiv - Molecular Biology 2021Quote: ... The constructs were screened via colony PCR and confirmed by sequencing (Sequencing Core Facility, SUNY, Albany, and Eaton Bioscience Inc. sequencing service) using the ACYCDuetUP1 (Novagen Cat. No.71178-3) and DuetDOWN1 primers (Novagen Cat ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was carried out in 20 µL reactions using 20 ng cDNA and SYBR® Green JumpStart™ ReadyMix™ (S4438, Sigma-Aldrich). ACTIN2 and TIP41 were used as reference genes and relative gene expression performed using 2−ΔΔCt method (Livak and Schmittgen ...
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bioRxiv - Neuroscience 2022Quote: ... 2.5 μl of the resulting cDNA was used as template in PCR performed in duplicates using SYBR Green dye (Sigma, St Louis, MO, USA). The PCR reactions were performed on CFX-96 C1000 Touch Real-Time System (Bio-Rad Laboratories ...