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Citations for Millipore Sigma :
2301 - 2350 of 2658 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... The resulting 5 kbp PCR fragments (Bio-DNA-DIG) (1.7 µm in length) reacted with neutravidin (Sigma) at a ratio of 100 neutravidin/DNA for 24 h at 4°C and were subsequently coupled to the anti-DIG beads at a reaction ratio of 150 neutravidin-DNA/bead for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (Sigma-Aldrich, USA) and sent for Sanger sequencing (Source Bioscience ...
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bioRxiv - Plant Biology 2021Quote: ... Open reading frames were PCR amplified (Phusion Hotstart II polymerase, ThermoFisher Scientific or KOD DNA polymerase, Millipore) from genomic DNA or BAC ...
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bioRxiv - Microbiology 2020Quote: ... All PCR used for cloning were performed with high fidelity KOD Hot Start DNA Polymerase (EMD Millipore). E ...
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bioRxiv - Neuroscience 2020Quote: ... DNA extraction was performed using the REDExtract-N-Amp Tissue PCR Kit (Sigma, St Louis, MO, USA).
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were PCR-amplified with 10-12 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and cleaned up using 1X KAPA Pure Beads ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative reverse transcription PCR reactions used the 2xKiCqStart® SYBR® Green qPCR ReadyMix™ (Sigma-Aldrich). PCR consisted of an initial activation step at 95°C for 3 min ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich, MA, USA), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were digested with NdeI and BamHI and inserted into the pET-15b plasmid (Novagen) digested with the same enzymes ...
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bioRxiv - Molecular Biology 2019Quote: Telomerase activity was detected using the PCR-based Telomeric Repeat Amplification Protocol (TRAP) assay kit (Millipore, S7700). Briefly ...
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bioRxiv - Plant Biology 2019Quote: ... The synthesized sequences were amplified via PCR and cloned into the pET-15b expression vector (Novagen, www.merckmillipore.com) using NdeI and BamHI restriction sites to create expression constructs with an N-terminal 6-His-Tag followed by a thrombin cleavage site (pET-15b-SAP54 ...
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bioRxiv - Cancer Biology 2019Quote: ... Genotyping PCR was performed from genomic DNA from tails using standard protocol of N-Extract kit (Sigma).
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bioRxiv - Microbiology 2020Quote: Total genomic DNA was extracted using the REDExtract-N-Amp™ Tissue PCR Kit (Sigma- Aldrich, USA), which yields high quantities of DNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... The PCR product was subcloned between the NdeI and KpnI sites of the pCOLADuet-1 (Novagen, USA) MCS-2 using the In-Fusion HD cloning kit.
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bioRxiv - Synthetic Biology 2021Quote: ... The PCR product was subcloned between the BamHI and NotI sites of the pCOLADuet-1 (Novagen, USA) multiple cloning site (MCS)-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were routinely tested for mycoplasma contamination using the Lookout Mycoplasma PCR Detection Kit (#MP0035; Sigma-Aldrich). Cells were treated with the proteasome inhibitor MG132 (ApexBio ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were tested periodically for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit from Millipore Sigma.
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA extraction was performed with a REDExtract-N-Amp Tissue PCR Kit (Sigma, St. Louis, MO), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were carried out using FastStart™ High Fidelity DNA polymerase (Sigma-Aldrich Cat no. 3553400001) in a reaction volume of 250 μl using 10 μl of the cDNA product as a template ...
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bioRxiv - Molecular Biology 2020Quote: ... An open-reading frame of the truncated Pch2 was PCR-amplified and inserted into pET15b plasmid (Novagen) in which an N-terminus of the PCH2 gene was tagged with hexa-histidine ...
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bioRxiv - Microbiology 2022Quote: ... the native encoding sequence was amplified by PCR and inserted into NdeI- and XhoI-digested pET36b(+) (Novagen). The fusion construct was verified by DNA sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... genotype was assessed with tail DNA extracted using RED Extract-N-Amp PCR Ready Mix (Sigma-Aldrich) followed by a polymerase chain reaction (PCR ...
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bioRxiv - Physiology 2022Quote: ... Each real-time PCR reaction mix was prepared with SYBR Green JumpStart Taq ReadyMix (Sigma, Oakville, Canada) and forward and reverse primer mix (3 µM ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were routinely tested for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (cat#MP0035, Sigma-Aldrich).
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bioRxiv - Immunology 2024Quote: ... Cell lines were confirmed to be mycoplasma-free with the Lookout Mycoplasma PCR Detection Kit (Sigma-Aldrich). Genotyping of various mice used in this work was accomplished with the APExBIO direct mouse genotyping kit and primers described in Table S1 ...
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bioRxiv - Biochemistry 2024Quote: ... The coding sequence of the DDX1 protein was PCR amplified and inserted into the pET28 vector (Novagen). A His-tag was placed in the N-terminal of DDX1 and a stop codon was inserted at the coding sequence of amino acid 708 ...
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bioRxiv - Biochemistry 2023Quote: ... All cells were examined negative for mycoplasma infection using PCR-based Mycoplasma Detection Kit (Sigma, MP0035-1KT). Mouse embryonic fibroblast cell (MEF ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were routinely tested for the mycoplasma absence using the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: Classical PCRs were performed using the RedTaq (WVR) and amplicons were visualized by SYBR Green stained (Sigma) agarose gel electrophoresis (1 to 2% agarose) ...
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bioRxiv - Developmental Biology 2023Quote: DNA was extracted from mice tail samples using Extract-N-Amp™ Tissue PCR Kit (Sigma, USA). PCR primers used for genotyping are listed below ...
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bioRxiv - Microbiology 2023Quote: ... All the cell types were confirmed free of mycoplasma using Lookout Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Neuroscience 2023Quote: Cells in culture were routinely tested for mycoplasma infection with Lookout Mycoplasma PCR detection kit (Sigma, MP0035), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... we subjected the amplified PCR products to electrophoresis using gels consisting of 2-3% agarose (Sigma Aldrich) in 1 x Tris-acetate-EDTA (1 x TAE ...
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bioRxiv - Genomics 2023Quote: ... Cells were routinely tested for free of mycoplasma by using LookOut Mycoplasma PCR detection kit (Sigma, #MP0035).
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... Blood DNA sample was prepared using Extract-N-Amp Blood PCR Kit (XNAB2R, Sigma, St. Louis, MO) and diluted 100 times ...
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bioRxiv - Molecular Biology 2024Quote: ... All cell lines were verified as mycoplasma free using the Lookout Mycoplasma PCR Detection Kit (Sigma-Aldrich) and genetic identities were validated by PCR ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were PCR-amplified with 12-13 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and cleaned up using 1X KAPA Pure Beads ...
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bioRxiv - Neuroscience 2024Quote: DNA was extracted from mice tail samples using Extract-N-Amp™ Tissue PCR Kit (Sigma, USA). PCR primers used for genotyping are listed below ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of mRNA was quantitatively assessed by real-time PCR using KAPA SYBR FAST (Sigma-Aldrich, KK4608) in a Biorad iCycler IQ (Biorad ...
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bioRxiv - Microbiology 2019Quote: ... and all cells were tested and found to be Mycoplasma-free using the LookOut PCR Detection Kit (Sigma).
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bioRxiv - Biochemistry 2020Quote: ... Venor GeM PCR-based mycoplasma detection kit was used as stated in the manual and was from Sigma. ECL substrate and ECL-Plus substrate were from Pierce and were used as directed ...
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bioRxiv - Plant Biology 2019Quote: ... 200µl supernatants transferred to a 96 well PCR plate and treated with 4U of almond beta-glucosidase (Sigma) at 37°C for 1.5 h ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted using the GenElute™ Mammalian Total RNA Miniprep Kit Q-PCR (Sigma, Cat#RTN350), and reverse transcription was performed using an oligoDT primer using superscript II (Thermofisher ...
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bioRxiv - Microbiology 2019Quote: All PCR reactions used for cloning were performed with high fidelity KOD Hot Start DNA Polymerase (EMD Millipore). Approximately 100 bp of upstream flanking DNA and the coding regions of OG1RF_10820 ...
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bioRxiv - Cell Biology 2021Quote: ... The two tensin fragments were amplified individually by PCR using KOD Xtreme™ Hot Start DNA Polymerase (Sigma) and were run on 1% agarose gel along with the linearized vector ...
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bioRxiv - Cell Biology 2021Quote: ... and Sox2 cDNA were amplified by real-time qPCR using a SYBR PCR Master Mix Kit (Sigma-Aldrich) and a 7500 Real-Time PCR Detection System (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... A plasmid encoding for TrxHis-Cobl-like1-411 was generated by PCR and subcloning into pET-32 (Novagen) (primers ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were monitored for mycoplasma using the LookOut mycoplasma PCR detection kit (Sigma-Aldrich, cat. MP0035). THP-1 positive controls for phospho-STING (S366 ...
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bioRxiv - Cancer Biology 2019Quote: Genotyping to determine p53-/- mice was performed with the REDExtract-N-AMP Tissue PCR Kit (Sigma XNAT-100RXN). Ear punches were digested according to the kit protocol ...
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bioRxiv - Zoology 2021Quote: ... Amplified PCR products were gel-extracted and purified with the Illustra Exo ProStar kit (Sigma Aldrich, Madrid, Spain) according to the manufacturer’s instructions ...