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Citations for Millipore Sigma :
2501 - 2550 of 2658 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ScMIC10-FLAG was amplified by PCR using the oligonucleotides 40/41 and integrated into the EagI/XbaI restriction sites of pFLAG-CMV5.1 (Sigma Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... AID Full Length F 5’-CCCAAGCTTATGGGCAGTGTCGAGCTG-3’ AID 1-114 F 5’-CCCAAGCTTATGGCAGTGTCGAGCTGAATC-3’ AID 31-114 F 5’-CCCAAGCTTAGAGGGTTCTCAGAGACGGTTG-3’ AID 71-114 F 5’-CCCAAGCTTAAAGATCCAGCCAAACCTCC-3’ AID R 5’-AAGGAAAAAAGCGGCCGCTACCTTCACGAACG-3’ PCR products were cloned into p3xFLAG-CMV10-PP6c construct (Sigma) using restriction digests and sequence confirmed ...
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bioRxiv - Immunology 2023Quote: The serotyped PCR products visualised under UV light were cut from the agarose gel using x-tracta Tool (Sigma Aldrich). The excised DNA fragments were further refined using nucleospin gel and by using PCR clean-up kit (Macherey-Nagel ...
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bioRxiv - Plant Biology 2023Quote: ... This gene was amplified by PCR and inserted via In-Fusion system (TOYOBO) into the NdeI and XhoI sites of pET22b(+) (Novagen). Primers are listed in Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... The polymerase chain reaction (PCR) was run using 0.2 U/μL of KOD Hot Start DNA polymerase (Novagen, Cat# 71316), 0.3 μM each of primers ...
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bioRxiv - Neuroscience 2023Quote: ... all cultures were monthly tested for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma, cat. no MP0035-1KT).
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bioRxiv - Cell Biology 2023Quote: ... the stonewall coding sequence was amplified by PCR and cloned into the XhoI and NcoI sites of the pET28a vector (Novagen). The plasmid was transformed into E.coli BL21(DE3 ...
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bioRxiv - Microbiology 2023Quote: ... Regular testing for mycoplasma contamination was performed to confirm contamination free culture using a PCR-detection kit (Sigma-Aldrich/Merck). The details of cell lines are provided in key resources table ...
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bioRxiv - Microbiology 2024Quote: ... PCR reactions used to create DNA fragments for allelic exchange were performed using KOD Hot Start DNA Polymerase (EMD Millipore) and cloned into pLT0675 ...
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bioRxiv - Cancer Biology 2024Quote: ... profiling at Broad between January and December 2018 and were routinely tested for mycoplasma using LookOut Mycoplasma PCR Kit (Sigma)
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... DNA was extracted from the euthanized embryos using Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: The sequence for each nanobody was amplified by PCR from the yeast surface display vector and cloned into pET26b (Novagen) with a C-terminal hexahistidine tag by Gibson assembly ...
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bioRxiv - Molecular Biology 2024Quote: ... Mycoplasma contamination was tested to confirm contamination-free cell culture using a PCR-detection kit (Sigma-Aldrich, cat no# MP0035).
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bioRxiv - Biochemistry 2024Quote: ... coli including a polyhistidine tag and thrombin cleavage site at its N-terminus was amplified via PCR and subcloned into pET-30b (Novagen) between the NdeI/ BamHI sites (Vermot et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The cyclin D1-Flag plasmid was generated by PCR insertion of cyclinD1 into the p3XFLAG-CMV™-14 expression vector (Sigma). The XPack-GFP plasmid was generated by inserting GFP from pEGFP-N1 (BD Biosciences Clontech ...
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bioRxiv - Microbiology 2021Quote: ... Cell lines used in this study were negative for mycoplasma contamination as determined with Lookout Mycoplasma PCR Detection Kit (Millipore Sigma). During the course of this study ...
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bioRxiv - Biochemistry 2022Quote: ... and 3% hydrochloric acid followed by sample pooling via centrifugation at 1,500 rpm for 2 minutes to the proteoCHIP funnel part for direct injection or transferred to 0.2 mL PCR-tubes coated with 1e-3 % Poly(ethylene glycol) (95172-250G-F, Sigma-Aldrich, Germany).
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bioRxiv - Molecular Biology 2020Quote: ... Products from the initial PCR of these samples were then purified using the Ultrafree-DA centrifugal filter units (Millipore, Cork, Ireland); the purified products were cloned using the dual-colour selection Strataclone PCR cloning kit according to the manufacturer’s protocol (Stratagene ...
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bioRxiv - Molecular Biology 2020Quote: ... All cell lines tested negative for mycoplasma infection and were routinely tested afterwards using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich). A549 cells (ATCC CCL-185 ...
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... To construct pKD1 the NUP188 coding sequence was amplified by PCR and subcloned into p3XFLAG-CMV™-10 vector (Millipore Sigma) using the Gibson Assembly® Master Mix (NEB ...
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bioRxiv - Cell Biology 2019Quote: ... PCR products with the coding sequence were cloned into NdeI/XhoI or SalI/XhoI sites of pET30a (+) vector (Novagen, Darmstadt, Germany), respectively ...
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bioRxiv - Plant Biology 2020Quote: ... qRT–PCR was performed with the Bio-Rad CFX384 Real-Time PCR system using the SYBR Green Jumpstart Taq ReadyMix (S4438-100RXN; Sigma-Aldrich) with the following PCR conditions ...
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bioRxiv - Cell Biology 2021Quote: ... The gDNA extractions and PCR verifying integration of the fluorescent tags into the genomic loci was performed using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). PCR amplicons were run on 1 or 2% agarose gels in TBE and gels were imaged with Bio-Rad Gel ChemiDoc XRS system and Quantity One software.
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bioRxiv - Cell Biology 2021Quote: ... CDC50.3 and CDC50.4 were generated using a PCR fragment encoding the mAID–HA and the HXGPRT cassette produced using the KOD DNA polymerase (Novagen, Merck) with the vector pTUB1:YFP-mAID-3HA as template and the primers indicated in Table S2 ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Microbiology 2020Quote: ... Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich). Catalytically inactive Mt2 (Mt2 C78A ...
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bioRxiv - Microbiology 2020Quote: ... The 350 pb PCR product was digested and ligated into chloramphenicol-resistant pACD4K-C vector provided by the manufacturer (Sigma-Aldrich). The resulting recombinant pACD4K-C vector was transformed into Escherichia coli DH5a by heat shock ...
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bioRxiv - Microbiology 2020Quote: ... Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich) (Fig 4A) ...
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bioRxiv - Microbiology 2020Quote: ... Double-stranded DNA templates for each sgRNA were produced by template-free PCR with two partially overlapping oligos (PAGE-purified, Sigma-Aldrich). Where necessary ...
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bioRxiv - Neuroscience 2019Quote: cDNAs templates were synthesized from extracted total RNA using omniscript reverse transcriptase (Quiagen) and used to perform PCR amplification under the indicated conditions using Taq DNA polymerase (Sigma-Aldrich).
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bioRxiv - Plant Biology 2021Quote: Seeds were sown individually inside PCR tubes filled with 1X MS gel medium: 0.44% Murashige and Skoog (MS) Basal medium (Sigma-Aldrich, M5519), 0.5% sucrose ...
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bioRxiv - Cell Biology 2020Quote: ... All progenies of genetic crosses were verified by diagnostic PCR using KOD XTREME Hot Start DNA Polymerase (71975-3, EMD Millipore) or OneTaq® Hot Start Quick-Load® 2X Master Mix with Standard Buffer (M0488L ...
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bioRxiv - Systems Biology 2022Quote: ... and cDNA fragments were amplified by PCR according to the manufacturer’s protocol (GenomePlex Single Cell Whole Genome Amplification Kit, Sigma Aldrich WGA4). The amplified libraries were run on a bioanalyzer to assess quality (Agilent ...
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bioRxiv - Cell Biology 2022Quote: ... ear notches were collected from mouse pups to extract genomic DNA using the RED Extract-N-Amp Tissue PCR Kit (Sigma-Aldrich). Ear notches were incubated in the tissue preparation (25 μl/sample ...
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bioRxiv - Biochemistry 2022Quote: ... aeruginosa PAO1 served as a template for the amplification of the genes of interest via PCR using the KOD Xtreme polymerase (Novagen/Merck) and cloning primers containing the restriction sites (Eurofins Genomics) ...
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bioRxiv - Developmental Biology 2020Quote: The LINC00261 wild type cDNA was PCR-amplified from pENTR/D-TOPO-LINC00261 (gift from Leo Kurian) with KOD Xtreme™ DNA Hotstart Polymerase (Millipore). The resulting PCR product was inserted into pRRLSIN.cPPT.PGK-GFP.WPRE via its appended BshTI/SalI cloning sites ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were PCR-amplified in 40 μL reactions with 15-18 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and 500 nM of each primer P1.3 and P2.1 (synthesized by IDT ...
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bioRxiv - Molecular Biology 2019Quote: ... an EcoRI restriction site was removed by performing a PCR on AT1.03 and AT1.03R122KR126K pDRF-GW using KOD polymerase (Merck-Millipore, Burlington, Massachusetts, USA) with the forward primer 5’-ATACTAGTGCTAGCTCTAGACTCGAGTATGGTGAG-3’ and reverse primer 5’-ATAGCGGCCGCTGATCAGCGGTTTAAACTTAAGC-3’ ...
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bioRxiv - Microbiology 2021Quote: ... Expression of FLAG-tagged DNMT2 in fly cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich, 1:3000 dilution in 2% Milk in 1X TBS + 1% Tween-20) ...
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bioRxiv - Pathology 2022Quote: ... Pf2-HA_OE and pf2-HA_KO ChIP and input control samples) was recovered from Proteinase K treated samples using the GenElute PCR purification kit (Sigma-Aldrich).
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bioRxiv - Plant Biology 2019Quote: ... Full-length GUN1 cDNA and N-terminal truncated versions as EcoRI-SalI PCR fragments were ligated into pET48b (Novagen, Wisconsin, USA) and transformed into E ...
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bioRxiv - Developmental Biology 2019Quote: ... Yolk sacs from each of the dissected embryos were collected and genomic DNA preparation was done using Extract-N-Amp tissue PCR kit (Sigma-XNAT2). Placenta tissues were collected in RLT buffer and RNA was extracted using RNAeasy Mini Kit (Qiagen – 74104) ...
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bioRxiv - Cancer Biology 2019Quote: ... digital PCR was carried out using a Biomark HD (Fluidigm) with a 65bp TaqMan assay for the housekeeping gene RPP30 (Sigma Aldrich) (7) ...
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bioRxiv - Developmental Biology 2019Quote: Genomic DNA samples were prepared using tail tissues or embryonic tissues from the mice using the REDExtract-N-Amp Tissue PCR kit (Sigma-Aldrich). Genotyping was done using REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... was tagged with 6xHis at the C-terminus using PCR amplification and the modified Tau-His cassette was introduced into pET3a plasmid (Novagen, DE) linearized with NdeI enzyme succeeding in pET3a/htau40/6xHis plasmid for the protein expression in E.coli ...
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bioRxiv - Molecular Biology 2020Quote: The 3×Flag-CPEB3 plasmid was generated by subcloning the PCR-amplified human CPEB3 cDNA into the p3×FLAG-CMV 10 vector (Sigma-Aldrich). The human CPEB3 cDNA was synthesized using the indicated primers ...
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bioRxiv - Genomics 2020Quote: ... The numbers of amplification cycles needed for library preparation of each sample was then determined by quantitative PCR as follows: 25 µl SYBR Green Jumpstart Taq ReadyMic (Sigma-Aldrich), 5µl PCR product from previous reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... Gel-electrophoresis was used for the detection of PCR products (MYC 630 bp, Neu 386 bp, rtTA 380 bp) on 1,5% agarose (Sigma, A9539-500G) gel with Ethidium bromide solution (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... Expression of both FLAG-tagged AaDNMT2 in mosquito cells was confirmed using qRT-PCR and Western Blots using an anti-FLAG monoclonal antibody (SAB4301135 - Sigma-Aldrich, 1:3000 dilution in 2% Milk in 1X TBS + 1% Tween-20 ...
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bioRxiv - Microbiology 2019Quote: ... Primers (Table S1) used for PCR amplification of the beta-lactams genes (blaTEM, blaSHV, blaCXT-M and blaOXA) were acquired from Sigma Aldrich, USA ...