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Citations for Millipore Sigma :
2151 - 2200 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Cell lines were tested for mycoplasma using a PCR assay (Venor GeM Mycoplasma Detection Kit, Sigma).
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bioRxiv - Cell Biology 2023Quote: ... Cells were regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma, MP0035), using JumpStart Taq DNA Polymerase (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Each 20 µl PCR reaction contained 10 µl of JumpStart RedTaq DNA Polymerase Master Mix (Sigma), 8 µl dH2O ...
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bioRxiv - Synthetic Biology 2023Quote: ... Splicing by overlap extension PCR was performed with the KOD Xtreme Hot Start DNA Polymerase (Novagen). Generally ...
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bioRxiv - Genomics 2023Quote: ... The amplified cDNA was purified using the HighPrep PCR Clean-up System (Sigma Aldrich AC-60050) adding a volume of magnetic beads equal to 0.8x the sample volume ...
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were tested every 6-12 months either by PCR (LookOut kit, Sigma/Merck) or Mycostrips (Invivogen ...
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bioRxiv - Microbiology 2024Quote: ... The PCR reaction mixture consisted of Kappa Probe Fast qPCR Master Mix (2X) Kit (Sigma-Aldrich), 0.75 μL of primers and 2.5 μL of RNA in a final volume of 10 μL reaction ...
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bioRxiv - Microbiology 2023Quote: ... PCRs were performed in 25 µl with JumpStart RED Accutaq LA polymerase (Sigma-Aldrich, cat# D8045) in the supplied buffer ...
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bioRxiv - Immunology 2023Quote: ... Cells were continuously verified as mycoplasma free using the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Plant Biology 2024Quote: ... PCR-grade H2O and RedTaq Ready Mix according to the manufacturer’s instructions (Sigma-Aldrich, Rehovot, Israel). The amplified products were subjected to electrophoresis on 1.2% agarose gel containing agarose in 0.5 X TBE buffer and were stained with ethidium bromide ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments encoding human Kid and mNeonGreen were amplified by PCR and assembled into pFastbac1 (Novagen) by Gibson assembly as described (42) ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR product was then mixed with 1/3 volume 10 M ammonium acetate (Sigma, A1542) and 2 volume ethanol (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... The second PCR products were cleaned up using SpeedBeads™ magnetic carboxylate (obtained from Sigma Aldrich). Cleaned PCR2 products were quantified using Qubit® dsDNA HS Assay Kit (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... All cell lines were confirmed mycoplasma free using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Genomics 2021Quote: ... The total number of cells in each field was determined by counterstaining cell nuclei with 4,6-diamidine-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich; 50 mg/ml in PBS for 15 min at RT). Immunostained cells were mounted in Aqua-Poly/Mount (Polysciences ...
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bioRxiv - Genomics 2022Quote: ... The total number of cells in each field was determined by counterstaining cell nuclei with 4,6-diamidine-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich; 50 mg/ml in PBS for 15 min at RT). To improve the efficiency of Tbr2 detection ...
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bioRxiv - Developmental Biology 2024Quote: ... The total number of cells in each field was determined by counterstaining cell nuclei with 4,6-diamidine-2-phenylindole dihydrochloride (DAPI; Sigma-Aldrich; 50 mg/ml in PBS for 15 min at RT). Immunostained cells were analyzed via confocal microscopy using a Nikon A1R+ ...
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bioRxiv - Biophysics 2019Quote: ... DBNL(1-256) and DBNL(1-374) were amplified by PCR and cloned into pET28a vector (Novagen) between BamHI and NotI sites to obtain constructs for expression of his-tagged proteins in E ...
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bioRxiv - Genetics 2021Quote: ... cells tested for several strains of mycoplasma contamination using LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich #D9307).
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bioRxiv - Microbiology 2019Quote: ... the plasmid containing the insert was linearized and purified with Gene-Elute PCR Clean-Up Kit (Sigma). DNA concentration was measured with Qubit (ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative PCR mixtures (10 μL) contained 5 μL Kapa SYBR FAST qPCR Master Mix (2X) (Sigma-Aldrich), 300 nM of the primer pair ...
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bioRxiv - Evolutionary Biology 2019Quote: ... cDNA was subjected to qRT-PCR using SYBR® Green JumpStart™ Taq ReadyMix™ (Sigma-Aldrich) in a CFX96 Touch™ Real-Time PCR Detection System (Biorad ...
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bioRxiv - Microbiology 2020Quote: ... at 100 V for 25 min and purified with either GenElute PCR Clean-Up Kit (Sigma Aldrich) or with Zymoclean Gel DNA Recovery Kit (Zymo Research ...
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bioRxiv - Neuroscience 2021Quote: DIG-labeled DNA probes were synthesized using Roche PCR DIG Probe Synthesis Kit (Millipore Sigma ca#11636090910). Probes were designed to target either the LexA coding sequence (Probe 1 ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines tested and confirmed to be mycoplasma free using the Mycoplasma PCR ELISA kit (Sigma). CENP-A overexpression was induced by the addition of Dox to typical growth media at 10ng/ml (considered 1X) ...
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bioRxiv - Biophysics 2020Quote: ... The resulting 5 kbp PCR fragments (Bio-DNA-DIG) (1.7 µm in length) reacted with neutravidin (Sigma) at a ratio of 100 neutravidin/DNA for 24 h at 4°C and were subsequently coupled to the anti-DIG beads at a reaction ratio of 150 neutravidin-DNA/bead for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... purified with the Illustra™ GFX™ PCR DNA and Gel Band Purification Kit (Sigma-Aldrich, USA) and sent for Sanger sequencing (Source Bioscience ...
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bioRxiv - Plant Biology 2021Quote: ... Open reading frames were PCR amplified (Phusion Hotstart II polymerase, ThermoFisher Scientific or KOD DNA polymerase, Millipore) from genomic DNA or BAC ...
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bioRxiv - Microbiology 2020Quote: ... All PCR used for cloning were performed with high fidelity KOD Hot Start DNA Polymerase (EMD Millipore). E ...
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bioRxiv - Neuroscience 2020Quote: ... DNA extraction was performed using the REDExtract-N-Amp Tissue PCR Kit (Sigma, St Louis, MO, USA).
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were PCR-amplified with 10-12 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and cleaned up using 1X KAPA Pure Beads ...
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bioRxiv - Plant Biology 2020Quote: ... Quantitative reverse transcription PCR reactions used the 2xKiCqStart® SYBR® Green qPCR ReadyMix™ (Sigma-Aldrich). PCR consisted of an initial activation step at 95°C for 3 min ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were purified using the kit Gene eluteTM Gel extraction kit (Sigma Aldrich, MA, USA), re-surveyed on Nanodrop® and delivered for Sanger DNA sequenced at Eurofins Genomics (Ebersberg ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were digested with NdeI and BamHI and inserted into the pET-15b plasmid (Novagen) digested with the same enzymes ...
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bioRxiv - Molecular Biology 2019Quote: Telomerase activity was detected using the PCR-based Telomeric Repeat Amplification Protocol (TRAP) assay kit (Millipore, S7700). Briefly ...
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bioRxiv - Plant Biology 2019Quote: ... The synthesized sequences were amplified via PCR and cloned into the pET-15b expression vector (Novagen, www.merckmillipore.com) using NdeI and BamHI restriction sites to create expression constructs with an N-terminal 6-His-Tag followed by a thrombin cleavage site (pET-15b-SAP54 ...
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bioRxiv - Cancer Biology 2019Quote: ... Genotyping PCR was performed from genomic DNA from tails using standard protocol of N-Extract kit (Sigma).
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bioRxiv - Microbiology 2020Quote: Total genomic DNA was extracted using the REDExtract-N-Amp™ Tissue PCR Kit (Sigma- Aldrich, USA), which yields high quantities of DNA ...
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bioRxiv - Synthetic Biology 2021Quote: ... The PCR product was subcloned between the NdeI and KpnI sites of the pCOLADuet-1 (Novagen, USA) MCS-2 using the In-Fusion HD cloning kit.
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bioRxiv - Synthetic Biology 2021Quote: ... The PCR product was subcloned between the BamHI and NotI sites of the pCOLADuet-1 (Novagen, USA) multiple cloning site (MCS)-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were routinely tested for mycoplasma contamination using the Lookout Mycoplasma PCR Detection Kit (#MP0035; Sigma-Aldrich). Cells were treated with the proteasome inhibitor MG132 (ApexBio ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were tested periodically for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit from Millipore Sigma.
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bioRxiv - Molecular Biology 2021Quote: ... Genomic DNA extraction was performed with a REDExtract-N-Amp Tissue PCR Kit (Sigma, St. Louis, MO), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... PCR reactions were carried out using FastStart™ High Fidelity DNA polymerase (Sigma-Aldrich Cat no. 3553400001) in a reaction volume of 250 μl using 10 μl of the cDNA product as a template ...
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bioRxiv - Molecular Biology 2020Quote: ... An open-reading frame of the truncated Pch2 was PCR-amplified and inserted into pET15b plasmid (Novagen) in which an N-terminus of the PCH2 gene was tagged with hexa-histidine ...
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bioRxiv - Microbiology 2022Quote: ... the native encoding sequence was amplified by PCR and inserted into NdeI- and XhoI-digested pET36b(+) (Novagen). The fusion construct was verified by DNA sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... genotype was assessed with tail DNA extracted using RED Extract-N-Amp PCR Ready Mix (Sigma-Aldrich) followed by a polymerase chain reaction (PCR ...
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bioRxiv - Physiology 2022Quote: ... Each real-time PCR reaction mix was prepared with SYBR Green JumpStart Taq ReadyMix (Sigma, Oakville, Canada) and forward and reverse primer mix (3 µM ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... Blood DNA sample was prepared using Extract-N-Amp Blood PCR Kit (XNAB2R, Sigma, St. Louis, MO) and diluted 100 times ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were routinely tested for the mycoplasma absence using the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).