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Citations for Millipore Sigma :
2551 - 2600 of 2658 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Cultures reaching ≥ 1 × 105 cells·ml−1 had 35 ml of the 50 ml volume filtered for identification via 16S rRNA gene PCR onto 25 mm 0.22-μm polycarbonate filters (Millipore, Massachusetts, USA). DNA extractions were performed using the MoBio PowerWater DNA kit (QIAGEN ...
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bioRxiv - Molecular Biology 2020Quote: ... Only well separated bands corresponding to the main PCR products obtained were cut out from the gel and PCR products were extracted from gel slices using GenElute Agarose Spin Columns (Sigma-Aldrich). The PCR products were then ethanol precipitated ...
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bioRxiv - Cell Biology 2021Quote: ... the 5’ sequence of the native promoter and amino acids 1-443 of HEH1 were amplified via PCR (KOD polymerase, EMD Millipore) from genomic DNA using primers encoding the restriction sites BamHI and HindIII ...
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bioRxiv - Microbiology 2019Quote: ... All cell lines tested negative for mycoplasma infection and were routinely tested afterwards using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2020Quote: ... A 245-base fragment of the end of the mto2 coding sequence was amplified from cps1-191 (containing the mto2S338N mutation) with flanking homology to the pFA6a vector using the ExpandTM High Fidelity PCR system (Millipore Sigma). The vector backbone was amplified from pFA6a-GFP-KanMX6 using Phusion polymerase (New England BioLabs) ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines including stable clones were routinely evaluated for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma-Aldrich) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2022Quote: ... Ubi:caSMAD3 was cloned by PCR amplifying human constitutively active SMAD3 from Smad3 pCMV-SPORT6 (Harvard Plasmid Repository #HSCD00339271, Boston, MA, USA)(Sigma 11732641001), gel purifying (Qiagen #28604 ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids were then extracted and used as template in ~400 bp NGS amplicon generation PCR reactions using KOD Xtreme Hot Start DNA Polymerase (EMD Millipore). The amplicons were gel-extracted using the Monarch DNA Gel Extraction kits (NEB) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Indexing PCR reactions were pooled prior to magnetic bead clean-up with Sera-Mag SpeedBeads ™ carboxylate-modified hydrophilic (Sigma-Aldrich). An initial bead concentration of 0.5X was used to remove long fragments that are likely to represent contamination from fresh DNA and libraries were selected with a bead concentration of 1.8X to size select the expected library range of ~300 bp ...
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bioRxiv - Microbiology 2022Quote: ... Cells were verified using the Promega GenePrint STR kit (DNA Analysis Facility, Duke University) and as mycoplasma free by the LookOut Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... The genomic region surrounding the CRISPR target site for SOX21 was amplified by PCR (KOD Xtreme Hot Start DNA Polymerase; EMD Millipore), and products were treated with SURVEYOR nuclease (SURVEYOR Mutation Detection Kit ...
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bioRxiv - Neuroscience 2023Quote: ... and fed with StemFlex media (ThemoFisher Scientific) and undergo monthly mycoplasma testing to ensure cultures are mycoplasma-free with the LookOut Mycoplasma PCR detection kit (Millipore Sigma). For the differentiation of iPSC-derived microglia-like cells ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell lines tested negative for mycoplasma contamination using a PCR-based method using the VenorGeM Classic Mycoplasma Detection Kit (Sigma-Aldrich). STR-genotyping was performed to confirm the identity of HeLa-JVM ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR sex genotyping reactions were performed in a final volume of 25 μl with the following reagents and volumes per sample: 12.5 μl KAPA2G Fast HotStart DNA Polymerase (5 U/μL) from the KAPA2G Fast HotStart PCR Kit (Sigma-Aldrich, KK5601), 1 μl of 12.5 μM Ube forward primer and 1 μl of 12.5 μM Ube reserve primer ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR was performed following a standard procedure: The reaction tubes were prepared by adding 10 µL of RED Extract (Sigma-Aldrich), 4 µL MilliQ water ...
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bioRxiv - Neuroscience 2023Quote: DNA from larval tails and juvenile and adult tail fins was extracted using the Extract-N-Amp Tissue PCR Kit (Millipore Sigma). After verifying SNP location with Sanger sequencing at Azenta Life Sciences ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were confirmed to be free of mycoplasma contamination (VenorTMGeM Mycoplasma Detection Kit, PCR-based, Sigma Aldrich Cat #MP0025). Cells were grown in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Developmental Biology 2023Quote: ... Truncated FBF expression constructs were generated by PCR from full-length FBF-1 or FBF-2 and inserted into pETDuet-1 plasmid (EMD Millipore). Full-length C ...
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bioRxiv - Cancer Biology 2023Quote: ... All cells were regularly tested for mycoplasma contamination using the LookOut mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO, CA).
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bioRxiv - Developmental Biology 2023Quote: ... Library preparation was then performed using 200 ng of 4C-template DNA with the Expand Long Range PCR kit (Millipore, 4829042001) under specific PCR conditions (94 °C for 2 min ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T cells used for proteome-derived peptide library generation were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions (Fig ...
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bioRxiv - Cell Biology 2023Quote: ... Working stocks were screened to ensure the absence of mycoplasma every 3 months using LookOut Mycoplasma PCR Detection Kit (Cat. No. D9307 Sigma, UK). Cells were cultured in Modified Eagle’s Medium supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Genomics 2022Quote: ... Regions determined to be differential between the Illumina sequencing performed in 2017 and the PacBio sequencing performed in 2020 and 2021 were verified by polymerase chain reaction (PCR) using RedTaq (Sigma-Aldrich) according to standard protocols ...
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bioRxiv - Genetics 2022Quote: ... T4 ligations into multiple cloning site plasmids were performed by amplifying fragments via PCR with Roche Expand High Fidelity Polymerase (Sigma 11732641001) with primers containing 5’ restriction enzyme sequences for digestion following PCR purification ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial DNA was used to amplify the entire 16S region by PCR [5′-AGAGTTTGATCCTGGCTCAG-3′ (forward) and 5′-AAGGAGGTGATCCAGCCGCA-3′ (reverse)] using Expand High-Fidelity Polymerase (Sigma-Aldrich). The PCR products were purified using the QIAquick PCR Purification kit (Qiagen) ...
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bioRxiv - Cancer Biology 2023Quote: cDNA was diluted 10-50 times and quantified by real-time PCR with LightCycler® 480 SYBR Green I Master (SIGMA). The RPL11 ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR1 products for each tumor were individually column-purified with a GenElute™ PCR Clean-Up Kit (Sigma-Aldrich NA1020-1KT). For PCR2 ...
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bioRxiv - Biochemistry 2023Quote: ... 1 µL of diluted cDNA was mixed with 12,5 µL ReqTaq® Ready Mix™ PCR reaction mix (Sigma Aldrich, #R2523), 0,5 µL forward primer (0,2 µM) ...
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bioRxiv - Genomics 2023Quote: ... Homology sequences of 600-900bp flanking the cut site of C-terminal Yy1 were amplified by PCR from iXist-ChrX genomic DNA using FastStart High Fidelity enzyme (Sigma Aldrich). DNA sequence encoding the C-terminal 3xT7-2xStrepII-FKBP12F36V tag was amplified from a plasmid gifted by R ...
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bioRxiv - Cell Biology 2023Quote: ... elegans genomic DNA extracted from 8-10 adult worms using the Extract-N-Amp™ Tissue PCR Kit (Millipore Sigma, MA) as previously described38 then sequenced ...
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bioRxiv - Bioengineering 2023Quote: ... All the cell lines were regularly tested for mycoplasma contamination using the LookOut Mycoplasma PCR detection kit (Product no. #MP0035 Sigma-Aldrich) and maintained in humidified cell culture incubators at 37°C and 5% CO2.
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bioRxiv - Cell Biology 2023Quote: ... DNA was extracted from mice ear tissues (or tail for embryos) using Extraction « Extract-N-Amp PCR » kit (Sigma #XNAT2-1KT) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by neutralization with an equal volume of 40 mM Tris-HCl (pH 5.5).93 Multiplex polymerase chain reaction (PCR)-based genotyping was performed using REDTaq® polymerase per manufacturer recommendations (Millipore Sigma). Oligonucleotides were multiplexed as follows ...
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bioRxiv - Developmental Biology 2024Quote: ... or ear biopsies (3-week-old pups) was used for PCR based genotyping using the REDExtract-N-Amp kit (Sigma-Aldrich) with the primers listed in STable5 ...
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bioRxiv - Microbiology 2024Quote: ... The absence of mycoplasma contamination in the virus stocks was confirmed using the LookOut Mycoplasma PCR Detection Kit (Millipore Sigma; #MP0035).
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bioRxiv - Genomics 2020Quote: ... 1-4 PCR wells were combined in a tube and 40 μL of 97% 1H,1H,2H,2H-Perfluoro-1-octanol (Sigma-Aldrich #370533) was added ...
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bioRxiv - Molecular Biology 2019Quote: ... that amplify the coding sequence of the phytoene desaturase gene (tetur01g11270) (Table S1. PCRs were performed using the Expand Long Range dNTP Pack (Roche/Sigma-Aldrich, Belgium). Reaction mixtures were prepared according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: DIG-labeled riboprobes were generated using 2ul of PCR template with the Roche DIG RNA Labeling Kit (T7) (Sigma aldrich, SKU 11277073910)
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bioRxiv - Synthetic Biology 2020Quote: ... whilst AL-PHA assay plates were centrifuged 500 g for 10 minutes (MPS 1000 Mini PCR Plate Spinner, Sigma-Aldrich, MO, USA) in order to pellet AL-PHA beads ...
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bioRxiv - Cell Biology 2021Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was digested and with Nde1/Xho1 restriction enzymes and inserted into the bacterial expression vector pET-30a(+) (Novagen, Madison, WI) cut with the same enzymes ...
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bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
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bioRxiv - Neuroscience 2020Quote: Human cofilin1 was amplified by PCR using specific oligonucleotides (forward: 5’-CATATGGCCTCCGGTGTG-3’, reverse: 5’-GGATCCTCACAAAGGCTTGCCCTC-3’) and cloned into the pET-15b vector (Novagen, Millipore, UK). By using site-directed mutagenesis ...
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bioRxiv - Microbiology 2021Quote: ... A PCR master mix was prepared by combining the following reagent volumes per sample: 15 µl of Platinum PCR Supermix UDG (Sigma Aldrich, USA), 0.25 µl each of Forward and Reverse Primers (10 µM ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded on 96-well PCR plate and run in CFX real-time PCR System (Bio rad) using KiCStart SYBR Green qPCR ReadyMix with ROX as recommended by the manufacturer (Sigma-Aldrich: KCQS02). Three technical replicates were performed for each sample together with no-template control (NTC ...
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bioRxiv - Microbiology 2022Quote: ... All cell cultures and chlamydial stocks were routinely tested for Mycoplasma contamination using the Mycoplasma PCR detection kit (Sigma, St. Louis, MO). All chemicals and antibiotics were obtained from Sigma unless otherwise noted.
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bioRxiv - Microbiology 2022Quote: ... 500 base pairs of upstream and downstream sequence surrounding PBP-lipo (Rv2864c and MSMEG_2584c) were amplified by PCR KOD XtremeTM Hot Start DNA polymerase (EMD Millipore, Billerica, MA). These flanking regions were amplified with overlaps for the zeocin resistance cassette ...
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bioRxiv - Cell Biology 2022Quote: The determination of telomerase enzyme activity was done in HEKn cells by using TeloTAGGG Telomerase PCR Plus Kit (Sigma Aldrich, USA, 12013789001) according to the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2021Quote: ... The dried extracts were dissolved in 350 μl purified water and filtered through MultiScreen PCR-96 Filter Plate membranes (Merck Millipore, Darmstadt, Germany) to remove high-molecular-mass compounds ...
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bioRxiv - Cell Biology 2021Quote: HT29-18N2 cells (obtained from ATCC) (RRID:CVCL_5942) were tested for mycoplasma contamination with the Lookout mycoplasma PCR detection kit (Sigma-Aldrich, St. Louis, MO). Mycoplasma negative HT29-18N2 cells were used for the experiments presented here.