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Citations for Millipore Sigma :
2201 - 2250 of 2519 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Classical PCRs were performed using the RedTaq (WVR) and amplicons were visualized by SYBR Green stained (Sigma) agarose gel electrophoresis (1 to 2% agarose) ...
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bioRxiv - Developmental Biology 2023Quote: DNA was extracted from mice tail samples using Extract-N-Amp™ Tissue PCR Kit (Sigma, USA). PCR primers used for genotyping are listed below ...
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bioRxiv - Microbiology 2023Quote: ... All the cell types were confirmed free of mycoplasma using Lookout Mycoplasma PCR detection kit (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were PCR-amplified with 12-13 cycles using KOD Hot Start DNA polymerase (Millipore, 71086-3) and cleaned up using 1X KAPA Pure Beads.
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bioRxiv - Neuroscience 2023Quote: Cells in culture were routinely tested for mycoplasma infection with Lookout Mycoplasma PCR detection kit (Sigma, MP0035), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... All cell lines were verified as mycoplasma free using the Lookout Mycoplasma PCR Detection Kit (Sigma-Aldrich) and genetic identities were validated by PCR ...
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bioRxiv - Genetics 2023Quote: ... we subjected the amplified PCR products to electrophoresis using gels consisting of 2-3% agarose (Sigma Aldrich) in 1 x Tris-acetate-EDTA (1 x TAE ...
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bioRxiv - Genomics 2023Quote: ... Cells were routinely tested for free of mycoplasma by using LookOut Mycoplasma PCR detection kit (Sigma, #MP0035).
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... Blood DNA sample was prepared using Extract-N-Amp Blood PCR Kit (XNAB2R, Sigma, St. Louis, MO) and diluted 100 times ...
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bioRxiv - Immunology 2024Quote: ... Cell lines were confirmed to be mycoplasma-free with the Lookout Mycoplasma PCR Detection Kit (Sigma-Aldrich). Genotyping of various mice used in this work was accomplished with the APExBIO direct mouse genotyping kit and primers described in Table S1 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were routinely tested for mycoplasma using the LookOut Mycoplasma PCR Detection Kit (cat#MP0035, Sigma-Aldrich).
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bioRxiv - Biochemistry 2024Quote: ... The coding sequence of the DDX1 protein was PCR amplified and inserted into the pET28 vector (Novagen). A His-tag was placed in the N-terminal of DDX1 and a stop codon was inserted at the coding sequence of amino acid 708 ...
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bioRxiv - Microbiology 2019Quote: ... and all cells were tested and found to be Mycoplasma-free using the LookOut PCR Detection Kit (Sigma).
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bioRxiv - Biochemistry 2020Quote: ... Venor GeM PCR-based mycoplasma detection kit was used as stated in the manual and was from Sigma. ECL substrate and ECL-Plus substrate were from Pierce and were used as directed ...
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bioRxiv - Plant Biology 2019Quote: ... 200µl supernatants transferred to a 96 well PCR plate and treated with 4U of almond beta-glucosidase (Sigma) at 37°C for 1.5 h ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA was extracted using the GenElute™ Mammalian Total RNA Miniprep Kit Q-PCR (Sigma, Cat#RTN350), and reverse transcription was performed using an oligoDT primer using superscript II (Thermofisher ...
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bioRxiv - Microbiology 2019Quote: All PCR reactions used for cloning were performed with high fidelity KOD Hot Start DNA Polymerase (EMD Millipore). Approximately 100 bp of upstream flanking DNA and the coding regions of OG1RF_10820 ...
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bioRxiv - Cell Biology 2021Quote: ... The two tensin fragments were amplified individually by PCR using KOD Xtreme™ Hot Start DNA Polymerase (Sigma) and were run on 1% agarose gel along with the linearized vector ...
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bioRxiv - Cell Biology 2021Quote: ... and Sox2 cDNA were amplified by real-time qPCR using a SYBR PCR Master Mix Kit (Sigma-Aldrich) and a 7500 Real-Time PCR Detection System (Applied Biosystems ...
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bioRxiv - Cell Biology 2021Quote: ... A plasmid encoding for TrxHis-Cobl-like1-411 was generated by PCR and subcloning into pET-32 (Novagen) (primers ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were monitored for mycoplasma using the LookOut mycoplasma PCR detection kit (Sigma-Aldrich, cat. MP0035). THP-1 positive controls for phospho-STING (S366 ...
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bioRxiv - Cancer Biology 2019Quote: Genotyping to determine p53-/- mice was performed with the REDExtract-N-AMP Tissue PCR Kit (Sigma XNAT-100RXN). Ear punches were digested according to the kit protocol ...
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bioRxiv - Zoology 2021Quote: ... Amplified PCR products were gel-extracted and purified with the Illustra Exo ProStar kit (Sigma Aldrich, Madrid, Spain) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: The +2.0drl regulatory element was amplified from the zebrafish vector +2.0drl:EGFP by PCR using KOD Hot Start Master Mix (Novagen) (Table S1 ...
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bioRxiv - Developmental Biology 2019Quote: ... and this sequence was amplified by PCR from 3’ RACE cDNA using KOD Hot Start Master Mix (Novagen) with the following primers ...
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bioRxiv - Developmental Biology 2019Quote: ... marinus genomic DNA or from stl8-26 embryonic cDNA by PCR using KOD Hot Start Master Mix (Novagen). 3’ rapid amplification of cDNA ends (RACE ...
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bioRxiv - Biochemistry 2019Quote: ... the corresponding genes were amplified by PCR from S.cerevisiae genome and cloned into the plasmid pETDute-1 (Novagen). To construct N-terminal 6×His tagged Ups1-Mdm35 fusion protein ...
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bioRxiv - Biophysics 2019Quote: ... Mutagenesis was a carried out by a single PCR reaction using the KOD Hot Start DNA polymerase (Novagen) and appropriate mutagenic oligonucleotides (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... BamHI- and HindIII-digested FUBI or ubiquitin PCR fragments were cloned into a modified pET-28b(+) vector (Novagen) allowing for expression of a His6-tagged fusion protein with EGFP that was cloned into the HindIII and XhoI sites ...
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were tested to be free of mycoplasma contamination using a mycoplasma PCR detection kit (Sigma). Cell lines were authenticated using short tandem repeat (STR ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR products of control and sort2 were concentrated by using Amicon Ultra-0.5 10K (UFC501096, Merck Millipore), and analyzed by pair-end sequencing using NovaSeq 6000 system (Illumina) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell cultures were routinely checked for mycoplasma using the LookOut® Mycoplasma PCR Detection Kit (Sigma Aldrich, #D9307).
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bioRxiv - Evolutionary Biology 2021Quote: ... We used a high fidelity Taq DNA polymerase for DNA amplification (Expand High Fidelity PCR system from Sigma). After that ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was diluted 30-fold and 2.5µl was used to perform Quantitative PCR using Kapa SYBR fast Low-Rox (Sigma) in a final reaction volume of 7.5µl on a QuantStudio 5 384 PCR machine (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... pH 8.0 to a final concentration of 0.2nM and PCR amplified for cloning into T7 phage vector arms (Novagen/EMD Millipore Inc ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR fragments with appropriate restriction sites was cloned into the BamHI and SalI sites of pET28a (Novagen) and pGST4CS ...
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bioRxiv - Plant Biology 2022Quote: The full-length coding sequence of GGP1 was amplified by PCR and cloned into the pET28a vector (Novagen). The GGP1 enzyme was expressed in E ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were confirmed to be negative for mycoplasma using LookOut® Mycoplasma PCR Detection Kit (Sigma-Aldrich, MP0035).
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR was performed in triplicates with the KAPA SYBR® Fast qPCR Master mix (Sigma-Aldrich, KK4600) using a Rotor-Gene Q (Qiagen ...
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bioRxiv - Microbiology 2023Quote: Viral barcodes were amplified in 50 µl PCR reactions using KOD Hot-Start Master Mix (Sigma-Aldrich, 71842). For the viral supernatant samples ...
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bioRxiv - Pathology 2023Quote: ... Then 2.4 µL of multiplexed PCR products were mixed with 9.35 µL of Hi-Di formamide (Sigma-Aldrich) and 0.15 µL of Genescan 500 Liz internal line size standard (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... The PCR fragment of each miRNA was cloned into a pT7Blue T-Vector (Novagen, Inc, Madison, WI, USA). The recombinant plasmids were introduced into competent cells of Escherichia coli JM 109 ...
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bioRxiv - Biochemistry 2023Quote: ... Jurkat were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions (Fig ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Indexing PCR reactions were pooled prior to purification with Sera-Mag SpeedBeads™ carboxylate-modified hydrophilic (Sigma-Aldrich). An initial bead concentration of 0.5X was used to remove long fragments that were likely contaminants of fresh DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Mycoplasma testing was routinely performed using the LookOut® Mycoplasma PCR Detection Kit (Sigma, MA, USA, MP0035-1KT) to ensure the absence of contamination before cells were used in experiments.
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bioRxiv - Microbiology 2023Quote: ... Figure modified from D’Andreano & Francino27.Presence of PCR products was assessed using 0.5% agarose (Sigma-Aldrich, USA, A9539) gel electrophoresis with SYBR Safe DNA Gel Stain (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Cells were screened negative for mycoplasma contamination by monthly scheduled testing (LookOut® Mycoplasma PCR Detection Kit, Sigma). Stable cell lines expressing Sm.TRPMPZQ or Fh.TRPMPZQ were generated using the Flp-In T-REX core kit (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Offspring were screened for Taf7l mutations using REDExtract-N-AmpTM Tissue PCR kit (XNAT, Millipore Sigma, Burlington, MA) extracted genomic DNA from tail-tip biopsies ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse genotypes were determined by PCR using genomic DNA extracted using the REDExtract-N-Amp kit (Sigma XNAT) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Cells were inspected for mycoplasma contamination using a VenorTM GeM Mycoplasma Detection kit PCR-based (Sigma, MP0025-1KT).