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Citations for Millipore Sigma :
2351 - 2400 of 2658 citations for ssc mir 376c RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: The +2.0drl regulatory element was amplified from the zebrafish vector +2.0drl:EGFP by PCR using KOD Hot Start Master Mix (Novagen) (Table S1 ...
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bioRxiv - Developmental Biology 2019Quote: ... and this sequence was amplified by PCR from 3’ RACE cDNA using KOD Hot Start Master Mix (Novagen) with the following primers ...
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bioRxiv - Developmental Biology 2019Quote: ... marinus genomic DNA or from stl8-26 embryonic cDNA by PCR using KOD Hot Start Master Mix (Novagen). 3’ rapid amplification of cDNA ends (RACE ...
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bioRxiv - Biochemistry 2019Quote: ... the corresponding genes were amplified by PCR from S.cerevisiae genome and cloned into the plasmid pETDute-1 (Novagen). To construct N-terminal 6×His tagged Ups1-Mdm35 fusion protein ...
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bioRxiv - Biophysics 2019Quote: ... Mutagenesis was a carried out by a single PCR reaction using the KOD Hot Start DNA polymerase (Novagen) and appropriate mutagenic oligonucleotides (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... BamHI- and HindIII-digested FUBI or ubiquitin PCR fragments were cloned into a modified pET-28b(+) vector (Novagen) allowing for expression of a His6-tagged fusion protein with EGFP that was cloned into the HindIII and XhoI sites ...
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bioRxiv - Cell Biology 2020Quote: ... All cell lines were tested to be free of mycoplasma contamination using a mycoplasma PCR detection kit (Sigma). Cell lines were authenticated using short tandem repeat (STR ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR products of control and sort2 were concentrated by using Amicon Ultra-0.5 10K (UFC501096, Merck Millipore), and analyzed by pair-end sequencing using NovaSeq 6000 system (Illumina) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell cultures were routinely checked for mycoplasma using the LookOut® Mycoplasma PCR Detection Kit (Sigma Aldrich, #D9307).
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bioRxiv - Evolutionary Biology 2021Quote: ... We used a high fidelity Taq DNA polymerase for DNA amplification (Expand High Fidelity PCR system from Sigma). After that ...
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bioRxiv - Cell Biology 2021Quote: ... cDNA was diluted 30-fold and 2.5µl was used to perform Quantitative PCR using Kapa SYBR fast Low-Rox (Sigma) in a final reaction volume of 7.5µl on a QuantStudio 5 384 PCR machine (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... pH 8.0 to a final concentration of 0.2nM and PCR amplified for cloning into T7 phage vector arms (Novagen/EMD Millipore Inc ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR fragments with appropriate restriction sites was cloned into the BamHI and SalI sites of pET28a (Novagen) and pGST4CS ...
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bioRxiv - Biochemistry 2024Quote: ... The cell lines were tested for mycoplasma contamination using the LookOut® Mycoplasma PCR Detection Kit (Sigma Millipore) and were found to be negative for mycoplasma.
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bioRxiv - Biochemistry 2024Quote: ... The cell lines were tested for mycoplasma contamination using the LookOut® Mycoplasma PCR Detection Kit (Sigma Millipore) and were found to be negative for mycoplasma.
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bioRxiv - Microbiology 2024Quote: ... Each cell line was tested monthly for mycoplasma using the Lookout Mycoplasma PCR detection kit (Sigma-Aldrich, # MP0035) and confirmed mycoplasma free ...
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bioRxiv - Developmental Biology 2024Quote: ... Mouse genotypes were determined by PCR using genomic DNA extracted using the REDExtract-N-Amp kit (Sigma XNAT) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... mice DNA was extracted from ear notches or tails using the RED ExtractN-Amp Tissue PCR Kit (SIGMA). For Lrp1flox ...
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bioRxiv - Microbiology 2024Quote: ... Cells were inspected for mycoplasma contamination using a VenorTM GeM Mycoplasma Detection kit PCR-based (Sigma, MP0025-1KT).
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bioRxiv - Plant Biology 2022Quote: The full-length coding sequence of GGP1 was amplified by PCR and cloned into the pET28a vector (Novagen). The GGP1 enzyme was expressed in E ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were confirmed to be negative for mycoplasma using LookOut® Mycoplasma PCR Detection Kit (Sigma-Aldrich, MP0035).
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bioRxiv - Cell Biology 2023Quote: ... qRT-PCR was performed in triplicates with the KAPA SYBR® Fast qPCR Master mix (Sigma-Aldrich, KK4600) using a Rotor-Gene Q (Qiagen ...
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bioRxiv - Microbiology 2023Quote: Viral barcodes were amplified in 50 µl PCR reactions using KOD Hot-Start Master Mix (Sigma-Aldrich, 71842). For the viral supernatant samples ...
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bioRxiv - Pathology 2023Quote: ... Then 2.4 µL of multiplexed PCR products were mixed with 9.35 µL of Hi-Di formamide (Sigma-Aldrich) and 0.15 µL of Genescan 500 Liz internal line size standard (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... The PCR fragment of each miRNA was cloned into a pT7Blue T-Vector (Novagen, Inc, Madison, WI, USA). The recombinant plasmids were introduced into competent cells of Escherichia coli JM 109 ...
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bioRxiv - Cancer Biology 2023Quote: ... Mycoplasma testing was routinely performed using the LookOut® Mycoplasma PCR Detection Kit (Sigma, MA, USA, MP0035-1KT) to ensure the absence of contamination before cells were used in experiments.
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bioRxiv - Biochemistry 2023Quote: ... Jurkat were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions (Fig ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Indexing PCR reactions were pooled prior to purification with Sera-Mag SpeedBeads™ carboxylate-modified hydrophilic (Sigma-Aldrich). An initial bead concentration of 0.5X was used to remove long fragments that were likely contaminants of fresh DNA ...
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bioRxiv - Microbiology 2023Quote: ... Figure modified from D’Andreano & Francino27.Presence of PCR products was assessed using 0.5% agarose (Sigma-Aldrich, USA, A9539) gel electrophoresis with SYBR Safe DNA Gel Stain (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... Cells were screened negative for mycoplasma contamination by monthly scheduled testing (LookOut® Mycoplasma PCR Detection Kit, Sigma). Stable cell lines expressing Sm.TRPMPZQ or Fh.TRPMPZQ were generated using the Flp-In T-REX core kit (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... Offspring were screened for Taf7l mutations using REDExtract-N-AmpTM Tissue PCR kit (XNAT, Millipore Sigma, Burlington, MA) extracted genomic DNA from tail-tip biopsies ...
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bioRxiv - Microbiology 2024Quote: ... We confirmed the absence of mycoplasma contamination in cells using the LookOut Mycoplasma PCR Detection Kit (Millipore Sigma).
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bioRxiv - Biochemistry 2024Quote: ... Cells were tested every six months for mycoplasma contamination using the LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and oriT from pUC18T-mini-Tn7T-Tp-dsRedExpress were PCR amplified and introduced into a linearized pYES1L plasmid (Novagen) using the Gibson assembly method 46 (Supplementary Fig ...
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bioRxiv - Immunology 2021Quote: ... The gene sequence was amplified without the signal sequence and then PCR amplicons were ligated into the E.coli expression vector pET28a (Novagen) at the restriction sites shown in Table 1 ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative real-time PCR using cDNA was performed using KAPA SYBR fast qPCR master mix kit (Sigma Aldrich, USA) and Applied Biosystems StepOne Plus Real-Time PCR system ...
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bioRxiv - Microbiology 2019Quote: ... Size of the PCR products was verified by gel electrophoresis and purified using an Amicon Ultra 0.5 ml kit (Millipore).
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bioRxiv - Cell Biology 2020Quote: ... and 2μL of purified gDNA was added to 5μL of the 2X REDExtract-N-Amp PCR ReadyMix (Sigma-Aldrich), 0.2μL 10μM forward/reverse primer mix ...
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bioRxiv - Neuroscience 2020Quote: ... A 96-well PCR plate was prepared with one 3 mm diameter borosilicate solid-glass bead (Millipore Sigma #Z143928) and 100 µl PBS in each well ...
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bioRxiv - Cancer Biology 2021Quote: ... Coding region of H3.1 along with Flag-Tag was amplified by PCR using KOD Hot Start DNA Polymerase (Millipore) and cloned into gateway entry vector pENTR/D-TOPO vector (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR was performed with KiCqStart™SYBR®Green qPCR ReadyMix™(Sigma-Aldrich, St. Louis, MO, USA) using QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... while PCRs to generate specific knock-in constructs (mAID fusions and epitope tagging) were performed with KOD polymerase (Novagen). Specific gRNA were generated using the Q5 site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Immunology 2020Quote: ... PCRs for genotyping of bacterial colonies after transformation were performed using the KAPA2G Fast ReadyMix kit (Sigma Aldrich, #KK5102) with custom designed primers and the following cycling conditions ...
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bioRxiv - Cell Biology 2021Quote: ... Single endosteal and central marrow stromal cells were directly sorted into individual wells of a 96-well PCR plate in 2.3 μl of lysis buffer containing 0.2% Triton X-100 (Sigma-Aldrich) and 1U of Superase-In RNase Inhibitor (Ambion) ...
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bioRxiv - Neuroscience 2022Quote: ... The membrane was hybridized with a digoxigenin-labeled DNA probe generated with a PCR DIG Probe Synthesis Kit (Sigma). Hybridization with a 5’ probe produced an 8.1 Kb band from the WT and a 5.1 Kb band from the targeted locus ...
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bioRxiv - Microbiology 2022Quote: The PCR products were inserted into the pET-30 Ek/LIC vector according to manufacturer’s instructions (LIC Kit, Novagen) (Table S2) ...
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bioRxiv - Microbiology 2022Quote: ... the TAR vectors were PCR amplified from pCC1BAC-his3 with KOD Xtreme Hot Start DNA polymerase (Millipore, Burlington, MA) using the construction primers (labeled “Con” ...
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bioRxiv - Plant Biology 2021Quote: ... Half of the MET3 PCR product was digested for 1h30 at 37°C with XbaI restriction enzyme (Sigma-Aldrich). ACT2 amplification was used as a control.
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bioRxiv - Plant Biology 2021Quote: ... RNA was crosslinked using UV light and hybridized with a DIG labelled probe (PCR DIG probe synthesis kit, Sigma). For detection of LbuCas13a the membrane was stripped and probed with DIG labelled Cas13a specific probe and signal detected on a Licor Odyssey imaging system (LI-COR Bioscience ...
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bioRxiv - Plant Biology 2021Quote: ... a 2.25-kb fragment containing the PHOT1 CDS was amplified by PCR with KOD hot start DNA polymerase (Novagen) using PHOT1 FW and PHOT1 RV primers (Table 1) ...