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Citations for Millipore Sigma :
2301 - 2340 of 2340 citations for Clostridium Difficile Toxin B Ribotype 078 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... LC mobile phase solvents and sample dilutions used 0.1% formic acid in water (Buffer A) and 0.1% formic acid in acetonitrile (Buffer B) (Chromasolv LC–MS grade; Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Neuroscience 2024Quote: ... was kept as “soluble fraction.” The pellet (P1) was resuspended in Buffer B (10mM Tris-HCl, 850mM NaCl, 1Mm EGTA, 10% sucrose, (Sigma Aldrich, MO, USA) and centrifuged at 14,000 x g for 10min at 4°C to remove debris ...
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bioRxiv - Developmental Biology 2024Quote: 20-hydroxyecdysone (20-E, CAS-number: 5289-74-7) and cucurbitacin B (CucB, CAS-number: 6199-67-3) were obtained from Sigma-Aldrich (MA, USA). Based on our preliminary tests to check for lethal or critical effects on development ...
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bioRxiv - Neuroscience 2024Quote: ... supplied in four separate parts (Sigma-Aldrich, Ducupan Resin A/M, cat. no. 44611; Sigma-Aldrich, B hardener, cat. no. 44612; Sigma-Aldrich, D hardener cat ...
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bioRxiv - Cell Biology 2024Quote: ... The synthetic auxin α-Naphthaleneacetic acid (NAA) and dexamethasone (DEX) were obtained from Duchefa and latrunculin B (LatB) from Sigma-Aldrich (MO, USA). Except PI ...
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bioRxiv - Pathology 2022Quote: ... whereby the chromogen reagent solution was prepared fresh using 1 volume of the chromogen stock solution (1.86mM bathophenanthroline sulfonate-Sigma, #B-1375, 143mM thioglycolic acid-Sigma, #T-6750 in milliQ water), 5 volumes of saturated sodium acetate (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... harvested whole brains were cut and minced into small pieces and incubated in 10 mL Hanks’ Balanced Salt Solution (HBSS) with calcium and magnesium supplemented with 1 mg/mL collagenase B (Sigma-Aldrich, St. Louis, MO) at 37°C for 30 min with intermittent shaking ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL of filtered kanamycin sulfate (100 mg/mL, SERVA Electrophoresis #26899) and antifungal drug (50 μL of amphotericin B solution Sigma-Aldrich (250 μg/mL) #A2942 ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins in the bound (B) and unbound (UB)/flow through were analyzed by Western blotting using anti-GST rabbit polyclonal antibody (Sigma #G7781, 1:5000 dilution).
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bioRxiv - Neuroscience 2022Quote: ... where A was 0.1% formic acid (v/v) in water and B was 0.1% formic acid (v/v) in acetonitrile (all compounds were purchased at Sigma-Aldrich, St. Louis, MO, US). Elution gradient program for the analysis included a linear gradient of 5% to 95% solvent B at 7 min kept 95% B for 1 min ...
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bioRxiv - Immunology 2020Quote: ... Wapl-degron v-Abl pro-B lines were treated with 150 μM Indole-3-acetic acid (auxin analog, IAA, Sigma-Aldrich, #I3750-25G-A) and 2 mg/mL doxycycline (Dox ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were then transferred on a 125 x 4.0 mm phase 60RP-Select B LiChroCART 250-4 (Sigma-Aldrich St. Louis, MO, USA) and acetic acid was used as running buffer A ...
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bioRxiv - Neuroscience 2022Quote: ... with 690 μl Buffer B (50 mM Tris,pH 8, 10 mM NaCl) that contained 20% w/v glucose (Sigma Aldrich, cat. no. D9559). The buffer was prepared on ice.
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bioRxiv - Neuroscience 2022Quote: ... The thawing and culturing medium (NB Plus +) consisted of Neurobasal Plus Medium containing 2% B-27 Plus Supplement and 1% penicillin–streptomycin (PS; Sigma−Aldrich, cat. no. P0781). After thawing ...
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bioRxiv - Microbiology 2019Quote: B. burgdorferi strain B31 was cultured in BSK-H medium (HiMedia Laboratories Pvt. Ltd.) with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA). All culture medium was filter-sterilized by 0.2 µm filter ...
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bioRxiv - Immunology 2019Quote: ... 1.35 µg/ml Amphotericin B (all purchased from PAN-Biotech) by sedimentation and incubated overnight in RPMI-1640 supplemented with 1% Glucose (Sigma-Aldrich, St. Louis, US), 100 U/ml Penicillin ...
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bioRxiv - Plant Biology 2020Quote: Cells derived from roots of Arabidopsis thaliana (ecotype Columbia-0) were grown in 100 mL of Gamborg’s B-5 (Gamborg and Eveleigh, 1968) basal salt mixture (Sigma-Aldrich, St Louis, MO, USA) with 2,4-dichlorophenoxyacetic acid (0.5 µg mL-1 ...
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bioRxiv - Microbiology 2021Quote: ... strains were collected and isolated onto Humic Acid Vitamin Agar or Kings B Medium using a SASS-180 single-stage impact sampler (Bioscience International, Rockville, MD, USA) or an MAS-EC0100 (EMD Millipore, St. Louis MO) using published methods (94) ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit anti-BUBR1-p620 (custom raised by Moravian, 1:1000) and mouse anti-α-Tubulin (clone B-5-1-2, T5168, Sigma-Aldrich, 1:10000). Secondary antibodies used were goat anti-mouse IgG HRP conjugate (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... PS + 1% (v/v) B-27 + 1 μM ALK5 inhibitor (Stemgent, cat. no. 04-0015) + 10 μg/ml Heparin (Sigma-Aldrich, cat. no. H3149) + 25 ng/ml FGF7 + 10 μM Y-27632 ...
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bioRxiv - Cell Biology 2020Quote: ... after which cells were spheroplasted in 1ml of freshly prepared spheroplast buffer [Buffer B supplemented with 20mM ribonucleoside vanadyl complexes (Sigma-Aldrich, St. Louis, MO), 20mM β-mercaptoethanol ...
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bioRxiv - Immunology 2021Quote: ... To avoid bacterial infections mice received antibiotics-containing drinking water (2 g/l neomycin sulfate, 100 mg/l polymyxin B sulfate; Sigma-Aldrich, St. Louis, MO) for the first 5 days after infection ...
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bioRxiv - Microbiology 2020Quote: ... FFPE-tissues sections underwent deparaffinization with xylene and a series of ethanol washes and treatment with 0.1% Sudan Black B (Sigma-Aldrich, St. Louis, MO, USA) to reduce autofluorescence ...
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bioRxiv - Genomics 2022Quote: ... Cells were plated at 1 x 105 per well in a 6-well plate (FALCON, Cat.no. 353046) clonal density in 2i culture medium (15% FBS (Millipore, Cat. No. ES-009-B), 1X Glutamine (Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were washed three times for 10 min each at room temperature with 1x Duolink In situ Wash Buffer B (Sigma Aldrich, cat. no. DUO82049) followed by two washes of 2 minutes with 0.01x Duolink In Situ Wash Buffer B ...
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bioRxiv - Developmental Biology 2023Quote: ... Slides were dehydrated in 70% ethanol for two minutes and incubated in Sudan Black B (SBB) to block endogenous autofluorescent lipofuscin accumulation in the ovary (Sigma-Aldrich, 50395, 1:100) for five minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with primary antibody to stain tubulin (mouse anti-α-tubulin B-5-1-2 mAb, Sigma-Aldrich, T5168, dilution 1:250) for 4 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... fetal bovine serum and 0.05% (v/v) of an antibiotic and antimycotic mixture (penicillin, amphotericin B, and streptomycin; Sigma–Aldrich, St. Louis, MO, USA), designated hereafter as complete IPL-41 medium ...
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bioRxiv - Systems Biology 2024Quote: ... 1% pen/strep, 1% Glutamax, 50 μg/μL ascorbic acid [Sigma, St. Louis, MO, USA], 10 nM B-glycerophosphate [Sigma], 10 nM dexamethasome [Sigma]). After differentiation ...
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bioRxiv - Physiology 2020Quote: ... Silanized glass microelectrodes were then back-filled with 100 mmol L-1 KCl and front-filled with K+ ionophore (K+ ionophore I, cocktail B, Sigma Aldrich, St. Louis, MO, USA). A thinly pulled glass electrode (IB200F-4 ...
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bioRxiv - Physiology 2019Quote: ... Potassium-sensitive electrodes were backfilled with 100 mM of KCl and front-filled with K+ ionophore (K+ ionophore I, cocktail B, Sigma Aldrich, St. Louis, MO, USA). Sodium sensitive electrodes were backfilled with 100 mM NaCl and front-filled with Na+ ionophore (Na+ Ionophore II Cocktail A ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were treated with BCNU (125 μM) for 24 h before treating with cytochalasin-B (2 μg/mL) (Sigma Aldrich; Cat# 250233, St. Louis, MO) for an additional 24 h ...
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bioRxiv - Biochemistry 2020Quote: ... bovine pancreatic ribonuclease B (RNaseB), Fetuin, Dextran (Leuconostoc spp., Cat# 31388) and all other chemical reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fly powder ...
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bioRxiv - Microbiology 2020Quote: ... They were then resuspended in 250 µL of freshly prepared lysis solution (CelLytic B Cell Lysis Reagent 1x – Sigma -, Tris-HCl pH 7.5 (50 mM), PMSF (0.5 mM) ...
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bioRxiv - Bioengineering 2020Quote: ... extraction of the soluble fraction of the whole-cell lysate was done by chemical lysis using Cellytic™ B (Sigma-Aldrich, Saint Louis, MO, USA). Every ODU of cells was lysed using 16 µl of Cellytic™ B in 50 mM tris pH 8 ...
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bioRxiv - Genomics 2022Quote: ... In total 37 blastocysts were placed onto feeder (Applied Stem Cell, Cat. No. ASF-1014) monolayer cultures (2i medium - 15% FBS (Millipore, Cat. No. ES-009-B), 1X Glutamine (Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... The B-free nutrient solution (B0) was prepared by removing B from water using an ion exchange resin (Amberlite IRA743 free base, Sigma-Aldrich, St. Louis, MO, USA) over 3 d ...
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bioRxiv - Neuroscience 2023Quote: ... Enzymatic activity of beta-hexosaminidase (combined A and B isoforms) was determined by hydrolysis of 4-methylumbelliferyl-N-acetyl-β-glucosaminide (EMD Millipore Chemicals, Darmstadt, Germany) using 1.25 mM substrate in the incubation mixture ...
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bioRxiv - Physiology 2022Quote: ... Rhenen, The Netherlands). GM tissue and whole EDL muscles were lysed (Potter S 8533024, B. BRAUN) in RIPA buffer (Sigma-Aldrich, R0278, Saint Louis, MO, USA) containing 1 tablet of protease inhibitor (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... blocking was performed in 3% BSA in PEM80 for 45 minutes and incubated overnight with a primary antibody against αTub (mouse IgG1, Sigma Aldrich, B-5-1-2, dilution 1:500). The cells were again washed with PBS (3×5 min ...