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Citations for Millipore Sigma :
2201 - 2250 of 2340 citations for Clostridium Difficile Toxin B Ribotype 078 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... The bone sections were immediately sandwiched individually between two type B gold-coated copper freezer hats (BALTIC preparation, Wetter, Germany) with the addition of 10 wt% dextran (Sigma, 31390) as cryo-protectant ...
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bioRxiv - Biophysics 2023Quote: ... The remaining solution was removed from the wells and the cells were washed once with 1XTBS followed by incubation with 200μL of 0.2% (w/v) Janus green B (Sigma, Cat. no. 201677), a mitochondrial stain ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated in a primary rabbit α-human A3A/B/G antibody 1:1,000 (Brown et al.) and mouse α-tubulin 1:10,000 (Sigma Aldrich), or rabbit α-actin 1:5,000 (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... a stock solution [50 mg/ml JQ1 in dimethyl sulfoxide (DMSO)] was diluted to a working concentration of 5 mg/ml in an aqueous carrier (10% hydroxypropyl b-cyclodextrin; Sigma C0926) using vigorous vortexing ...
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bioRxiv - Neuroscience 2023Quote: ... then with primary antibodies (anti-LC3 A/B-12741, Cell Signaling, 1:1500 and anti-alpha tubulin-T6199, Sigma, 1:10000), followed by fluorescent secondary antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... Toxin was pressure injected using an inline Openspritzer47 based on initial calibration to 45 psi and 10 msec pulse duration by visualizing the injection volume of 128 μM sulfrhodamine B (Sigma-Aldrich). Following toxin injection ...
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bioRxiv - Synthetic Biology 2023Quote: ... HEK293T-LP cells with and without integrated libraries were maintained in DMEM supplemented with 50 µg/mL Hygromycin B (Sigma, H3274) and 1 µg/mL Puromycin (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacture’s protocol with the following modifications: a) RNA isolation was performed in the dark and b) DTT (Sigma Aldrich #43816) was added to the aqueous phase (final concentration 0.1 mM ...
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bioRxiv - Biochemistry 2023Quote: ... TREX complex was concentrated and subsequently cross-linked with either bis(sulfosuccinimidyl)suberate (BS3-d0/d12, Creative Molecules) in buffer B or with adipic dihydrazide (ADH-d0/d8, Sigma-Aldrich) and 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium (DMTMM ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-rabbit secondary antibodies, Ivermectin (I8898, diluted in 95% ethanol), and Leptomycin B (L2913, diluted in 100% ethanol) were purchased from Sigma Aldrich.
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore, H4 #07-108 Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore, H4 #07-108 Millipore, H3S28P #07-145 Millipore, VRK1 #HPA000660 Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with primary antibody (VRK3 #HPA056489 Sigma, B-actine #51255 Cell signaling, Lamin # ab16048 Abcam, H3S10P #05-806 Millipore, H4 #07-108 Millipore, H3S28P #07-145 Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... The insulin-secreting cell line INS-1 (a gift from Professor C. B. Wollheim) was cultured in RPMI-1640 (Sigma Aldrich), supplemented with 10% fetal bovine serum (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... The inlet reservoirs were filled with ECG medium. For microfluidic chip culture, Penicillin-Streptomycin Solution (Cat. #: 168-23191, Wako, Japan) and Amphotericin B (Cat. #: A2942, Sigma, USA) were added to the culture medium at a 1:100 dilution each ...
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bioRxiv - Neuroscience 2023Quote: ... washed with PBS/ Tween20 (buffer B) and then blocked with 1% bovine serum albumin in PBS/Tween20 (buffer C) (Sigma-Aldrich). 50 µL of mouse monoclonal V5B2 or rabbit polyclonal sPrPY226 ...
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bioRxiv - Physiology 2023Quote: ... Vein explants were plated on the dry surface of a cell culture plate coated with 1% Gelatine type B (Sigma-Aldrich). Explants were maintained in RPMI ...
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bioRxiv - Microbiology 2023Quote: Biofilms were rinsed vigorously with water and then treated with 1 ml of 100 μg/ml dispersin B (Kane Biotech) or 100 μg/ml bovine DNase I (Sigma-Aldrich) in saline ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of cell culture medium were incubated at 37°C for 3 hours with 20 µL reaction mix containing sodium citrate (10 mM; pH 4.2) and 4-methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (2 mM; #474502, Sigma-Aldrich/Merck). The reaction was stopped by 5 volumes of glycine and Na2CO3 ...
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bioRxiv - Genetics 2023Quote: ... or with a 1:4000 dilution of a mouse monoclonal anti-α-tubulin antibody (clone B 5-1-2) (Sigma Aldrich). The anti-Crym antibody is specific for an epitope that is located between amino acids 45 and 85 of the Crym protein ...
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... Dextran 70 kDa was used as a negative control and was also coupled to a rhodamine (Rhodamine B-isothiocyanate-labeled Dextran; R9379, Sigma- Aldrich).
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bioRxiv - Plant Biology 2024Quote: ... Transformants were selected with appropriate antibiotic(s) (hygromycin B, 20 µg mL-1; paromomycin, 10 µg mL-1; spectinomycin, 100 µg mL-1 Sigma-Millipore) for a week before screening them for fluorescence in 96 well plates ...
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bioRxiv - Microbiology 2024Quote: ... Sterile stocks of polymyxin B sulphate (PMB; Cat# 86-40302, Betapharma, Shanghai, China) were prepared in Milli-Q water (Millipore, USA) with filter sterilization using a 0.22-µm syringe filter (Sartorius ...
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bioRxiv - Developmental Biology 2024Quote: ... On the 7th day 1 µM Senexin B (SenB, Senex Biotechnology, Columbia, SC) or 1 µM 4-hydroxytamoxifen (4-OHT, Sigma-Aldrich) was added to the cells ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were washed twice with PBS and homogenized in PBS by polyethylene pestle (PES- 15-B-SI, Axygen) with purified water (18.2 MΩ; Millipore, Burlington, MA) treated with Chelex 100 (sigma ...
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bioRxiv - Neuroscience 2024Quote: ... The tip of the K+-sensitive barrel was filled with a valinomycin-based K+ ionophore (K+ ionophore 1, cocktail B, Sigma-Aldrich). The K+-sensitive microelectrodes were calibrated before each experiment with solutions containing 154 mM NaCl and 3 or 30 mM KCl ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 0-7h old females were acclimatised in the cups for 1h and sugar spiked with Rhodamine B (1mg/25ml of sugar; Sigma R6626) was presented for 2.5-3h at dusk ...
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bioRxiv - Microbiology 2024Quote: ... THP1-b monocytes were previously induced to differentiate into macrophages using 150 nM PMA (Phorbol 12-myristate 13-acetate; Sigma, P8139). Samples were fixed using Pierce 4% PFA (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... The chlorine concentrate solution was prepared by addition of a NaOCl tablet (B-Care Chemicals, Belgium) to 1 L ultrapure water (Milli-Q, Merck Millipore, Germany). The amount of free chlorine was quantified with the Pocket Colorimeter II (Hach,Belgium).
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bioRxiv - Biophysics 2021Quote: ... Fluorescently labeled actin was prepared by polymerizing G-actin at 5 mM in a 1:1 ratio with phalloidin–tetramethylrhodamine B isothiocyanate (phalloidin–TRITC – Sigma-Aldrich Co.). Polymerization of actin samples was induced by adding 1/10 volume fraction of 10 times concentrated F-buffer (20 mM sodium phosphate buffer pH 7.3 ...
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bioRxiv - Cancer Biology 2021Quote: Proliferation was assessed by either manual counting with trypan blue in triplicate or with Janus Green B (Sigma Aldrich #2869-83-2) with four technical replicates read on an Epoch2 (Biotek ...
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bioRxiv - Neuroscience 2021Quote: ... briefly dehydrated in 70% ethanol and then incubated in 0.1% Sudan Black B solution (Sigma Aldrich, ref. 199664, Saint Louis, MO, USA) at room temperature for 10 min ...
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bioRxiv - Cell Biology 2019Quote: ... For primary antibodies we used hybridoma supernatant of monoclonal antibody 6E3 Rat anti-CLASP2 (Maffini et al., 2009) at 1:150 and Mouse anti-α-tubulin clone B-512 (Sigma Aldrich) at 1:10000 ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed with 80% cold acetone and incubated with murine monoclonal anti-influenza A or B nucleoprotein (Millipore Billerica, MA, USA) followed by peroxidase-conjugated goat anti-mouse IgG (Kirkegaard and Perry Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies], 2 µg/mL heparin [Sigma-Aldrich] ...
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bioRxiv - Cancer Biology 2021Quote: Patupilone (epothilone B, Novartis, France), etoposide and cisplatin (Mylan, PA, USA), oxaliplatin (Sanofi-Aventis, France) and FX11 (cat. no. 427218; Merck Millipore, Lyon, France) were prepared in dimethylsulfoxide (DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse mAbs anti-Vinculin (hVIN-1) and anti-α Tubulin (clone B-5-1-2) and rabbit polyclonal anti-Paxillin (HPA051309) were from Sigma-Aldrich and were diluted 1:400 ...
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bioRxiv - Microbiology 2021Quote: ... and trimethylsilylated shortly before analysis through addition of 20 μl N,O-bis(trimethylsilyl)trifluoroacetamide with 1 % trimethylchlorosilane (BSTFA-TMCS, Cerilliant, Sigma B-023). The GC-MS instrumentation and settings (electron ionization mode ...
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bioRxiv - Microbiology 2019Quote: ... DT40 cells (immortalised chicken B cell line [16]) were maintained in Roswell Park Memorial Institute (RPMI) media supplemented with L-glutamine (Sigma-Aldrich, Merck), sodium bicarbonate (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... were cultured in T25 or T75 tissue culture flasks (TPP, Switzerland) that were coated with 0.1% gelatin solution (Millipore, ES-006-B). ES cells were passaged every 2 days with accutase (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... The primers used for the same were a) A350P forward 5’-gctgcatccggccgcatctcggc-3’ and b) A350P reverse 5’-gccgagatgcggccaaggatgcagc-3’ (Sigma Aldrich, India). Full length A350P was thereafter cloned into an empty pEGFP (Clontech ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... HEK293T cells were seeded in a 6-well plate so that they were 70-90% confluent the next day (∼0.8x10^6 - 1x10^6 cells/well in 2 mL of standard growth media containing 10% FBS (Millipore, TMS-013-B) and 1x Penicillin-Streptomycin (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... 1:100; anti-calbindin, CB38, Swant, 1:5000; anti-CT-B, #703, List Biological, 1:4000; anti-VGLUT1, AB5905, Millipore, 1:5000) were diluted in PBS-TX with 3% normal serum and sections were incubated in this cocktail for 48-60 hours at 4 ºC ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were maintained at a density of 0.2-1.5×105 cells / cm2 by passaging using TrypLE (12563011) every 24-48h on 0.1% gelatin-coated dishes (Millipore cat ES-006-B) at 37°C and 7% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... TMB was removed and cells were washed twice with 1XTBS followed by incubation with 0.2% Janus green B (Sigma, Cat. no. 201677) (w/v ...
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bioRxiv - Cancer Biology 2022Quote: Cell proliferation was assessed by the colorimetric assay of sulforhodamine B 2-(3-diethylamino-6-diethylazaniumylidene-xanthen-9-yl)-5-sulfo-benzenesulfonate (SRB) (Sigma Aldrich, USA)[25] ...
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bioRxiv - Cancer Biology 2022Quote: ... growth media was changed to media supplemented with 250 µg/ml Hygromycin B (Thermo Cat #10687010) or 10 µg/ml blastocidin S (Sigma, Cat #SBR00022) to select for stable pools as appropriate ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... SB was suspended in 10% 2-hydroxypropyl-b-cyclodextrin in sterile water and 2% dimethyl sulfoxide (Sigma-Aldrich, St. Louis, MO, USA). Three different group of rats were treated with SB as follows ...
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bioRxiv - Genomics 2021Quote: ... mESCs (J1) were initially cultured in presence of Mitomycin C inactivated mouse embryonic fibroblasts on 0.1% gelatin-coated (Millipore, ES-006-B) 6-well plates in Dulbecco’s Modified Eagle’s Medium (DMEM ...